InnovaPrep ACD-200 User manual

Copyright 2012-2018, InnovaPrep LLC www.innovaprep.com 1

Copyright 2012-2018, InnovaPrep LLC www.innovaprep.com 2
Table of Contents
1 Product Overview ................................................................................................................................. 3
1.1 Product Description .....................................................................................................................................3
1.2 Applications ................................................................................................................................................3 1.3
Materials Provided ......................................................................................................................................3
1.4 Materials Required But Not Provided...........................................................................................................4
1.5 Battery Power .............................................................................................................................................4
2 ACD-200 Bobcat Method of Operation .................................................................................................. 4
2.1 Sample Collection on an Electret Filter .........................................................................................................4
2.2 Wet Foam Elution........................................................................................................................................4 2.3
Foam Generation ........................................................................................................................................5
3 Components of the ACD-200 Bobcat ..................................................................................................... 6
3.1 Description of Components .........................................................................................................................6
3.2 Check Status Button and Associated LEDs ....................................................................................................7
4 Procedures ........................................................................................................................................... 8
4.1 Setting Up the ACD-200 Bobcat for Collection ..............................................................................................8
4.2 Optional Battery Operation .........................................................................................................................9
4.3 Installing the Filter .................................................................................................................................... 10
4.4 Collecting a Sample ................................................................................................................................... 10
4.5 Recovery of the Sample ............................................................................................................................. 11
4.6 Eluting a Filter Sample ............................................................................................................................... 11
4.7 Cleaning the System .................................................................................................................................. 11
4.8 Battery Charging ....................................................................................................................................... 12

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1 PRODUCT OVERVIEW
This section contains a product description of the InnovaPrep ACD-200 Bobcat™ Dry Filter Air Sampler and a brief look at
its applications.
1.1 PRODUCT DESCRIPTION
The InnovaPrep ACD-200 Bobcat, shown in Figure 1.1, is a lightweight, portable, dry
filter air sampler with a unique patented rapid filter elution system. The Bobcat
aerosol collector takes up little more than one-quarter of a cubic foot, has an internal
battery, built-in tripod, and can run continuously at 200 liters per minute for Up to 14
hours on continuous mode or 6 days on extended run when using the lithium ion
battery. The system has a built-in, omni-directional aerosol inlet, easy to read status
display, and a built-in handle. The system is equipped with a mass flow sensor that
allows for consistent sampling rates throughout the collection cycle. It can be
operated in four programmable run modes that allow the user to balance collection
rate with battery life. The system is easy to use and operators can be trained in a
little as 10 minutes. Installation of the filter cassettes and the controls can be
manipulated with gloved hands, while wearing personal protective equipment such as military-style
nuclear/biological/chemical (NBC) protective suits.
Following aerosol collection, the user snaps the Sample Cup onto the Filter to remove it from the Collector, then snaps the
Elutor onto the Filter. To extract the captured particles from the Filter, a canister containing the Elution fluid is pressed
into the Elutor. The Elution Fluid is released from the canister and turns to foam as it flushes the Filter. The wet foam
passes through the interstitial spaces of the Filter to efficiently extract captured particles. Sample elution takes
approximately 5 seconds and produces 6 to 7 milliliters of liquid sample. The foam quickly collapses back to a liquid in the
Sample Cup, making it available for sample processing and analysis.
1.2 APPLICATIONS
The InnovaPrep ACD-200 Bobcat is ideally suited for the collection of bioaerosols and particulate matter; including
submicron sized particles, airborne molecular contamination, and particulates. This system has been developed to address
a broad range of air sampling requirements. The Filter and Elution Kit are single-use, therefore there is no requirement for
decontamination between samples. Since no liquids are used in the collector, the system can be operated at temperatures
ranging from -20° C to +60° C in non-condensing environments.
1.3 MATERIALS PROVIDED
Inspect each component when unpacking. If damage is observed, do not use the instrument, and contact InnovaPrep
immediately. Remove any protective packaging that may be present around the instrument. Examine the packaging
material to be sure that materials that have been provided (see the list below) are removed. Please keep the custom foam
packaging insert for use in the event that a return shipment is necessary.
Component InnovaPrep P/N Quantity
InnovaPrep ACD-200 Bobcat
ACD-200
1
External Power Supply
AC01057
1
Power Cord
HC03019
1
FIGURE
1.1
ACD
-
200
BOBCAT

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Power/Trigger Cable Assembly
AC01129
1
1.4 MATERIALS REQUIRED BUT NOT PROVIDED
A Rapid Filter Elution Kit is required for sampling. Users have a choice of two
kits with different fluid types.
• Rapid Filter Elution Kit with TRIS - Item AC00201-T
• Rapid Filter Elution Kit with PBS - Item AC00201-P
Each Filter Elution Kit includes:
1. Filter
2. Sample Cup
3. Sample Cup Lid
4. Elutor
5. Elution Fluid Canister
These are available for purchase on the InnovaPrep website.
To operate the unit in “Trigger” mode you will need to purchase the Trigger Accessory.
1.5 BATTERY POWER
Battery is not included. Two rechargeable battery options are available for the ACD-200 Bobcat.
• 7.5 Ah Lithium Ion – Recommended for holding the longest charge (up to 12 hours on continuous mode). Item
AC01054
• 4.8 Ah Nickel Metal Hydride – Holds charge up to 7 hours on continuous mode. Item AC09006
2 ACD-200 BOBCAT METHOD OF OPERATION
The InnovaPrep ACD-200 Bobcat uses an electret filter to capture particles onto the surface of polymer fibers in the filter.
A novel Wet Foam Elution™ process is then employed to wash the particles off of the fiber surfaces into very small liquid
volumes.
2.1 SAMPLE COLLECTION ON AN ELECTRET FILTER
The system uses a dry 52 mm electret filter as the collection media. Electret filters are made with a combination of
positively charged and negatively charged fibers. This substantially increases the collection efficiency of the filter and
allows for the use of lower pressure drop filters, which, in turn, allows for higher sampling rates for extended periods using
battery power. Additional advantages include low consumable costs, ease of use, and high durability. It has the ability to
operate below 0°C — a limitation for most collectors.
2.2 WET FOAM ELUTION
The single-use kit comes with everything you need to recover a fluid sample, including the Filter, a Sample Cup with Lid,
an Elutor, and a canister of Elution Fluid. The patented Wet Foam Elution process (US Patent 8,100,112; 8,958,623;
9,738,918) is much more efficient than liquid rinsing for the following reasons:
2
3
1
4
5

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• Volume Expansion
When rinsing a filter with liquid, most of the liquid volume is used to fill the dead space inside the filter housing;
only a small portion of the fluid is actually in contact with the filter surface. This can be minimized to an extent by
reducing the cross-sectional area of the fluid path across the filter, but a large portion of the liquid is still
underutilized. However, foam is 80-90% gas, which fills the empty space without contributing to the final sample
volume.
• Increased Viscosity
Liquid has a tendency toward “channeling” when flowing through a filter, that is, there is an area of high flow in
the center of the fluid path while the portion of flow in contact with the filter surface is much slower. The higher
viscosity of foam prevents channeling and creates a more uniform flow through the interstitial spaces of the filter.
• Bubble Dynamics
The micro-bubbles in the foam behave as deformable solids. As they travel through the filter they move as a rigid
body with a narrow lubricating layer, effectively squeegeeing the particles off of the surfaces of the filter fibers.
• Exfoliating Action
As the micro-bubbles in the foam impact against each other and burst, the turbulence and energy produced helps
to lift particles that are adhering to the filter fibers.
• Buffered Elution
The buffered elution fluid is conductive and breaks down the electrostatic charges holding the particles to the
electret filter, effectively releasing them in the liquid sample.
2.3 FOAM GENERATION
The Wet Foam Elution process uses a very specific high-quality foam to elute the filter. The elution fluid is composed of
water, a low concentration surfactant (0.075% Tween 20), and a pH buffer (Tris or PBS). This solution is carbonated at 130
psi, which dissolves significant amount of CO2 into the fluid. During the elution process, the valve opens as the elution
canister is pushed into the Elutor that is fitted onto the Filter, forcing the elution fluid through the pressure orifice, through
the filter and into the Sample Cup. As the fluid passes from the high-pressure environment on one side of the orifice, to
the low-pressure environment on the other side, the dissolved CO2 expands, comes out of the solution to form micro-
bubbles. These micro-bubbles increase the volume of the fluid six-fold or more as it passes through the filter. The foam
immediately collapses back into a liquid and is ready for sample processing and analysis.
3 COMPONENTS OF THE ACD-200 BOBCAT
The following section describes the components of the InnovaPrep ACD-200 Bobcat air sampler.

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3.1 DESCRIPTION OF COMPONENTS
Description of the InnovaPrep ACD-200 Bobcat components listed in Figure 3.1, clockwise from top:
• Lid Release – Used to open the ACD-200 for loading or recovering a filter cartridge.
• Power and Trigger Port - Inlet for the included Power and for the optional Trigger Connector.
• Leg Hinges with Locking Push Pins – Releasing the push pins allows the user to fold the legs up against the unit for
compact storage and convenient transportation.
• Telescoping Tripod Legs – Each of the three legs consist of three segments that allow the user to raise and lower
the unit to the desired height.
• Leg Latches – Each leg has three leg latches that allow the user to telescope the legs to the desired length.
• Battery Cover – Located on the bottom of the unit, the battery cover holds the battery in place during operation.
 Check Status Button – Used to verify the status of the system.
FIGURE
3.1
COMPONENTS OF THE AC
D
-
200
BOBCAT
Lid Release
Carrying Handle
Leg Hinges with Locking
Push Pins
Telescoping
Tripod
Legs
Power
and Trigger
Port
Mode Selection Knob
Check Status Button
Status Lights
Inlet Cap
Battery Cover
Leg Latches

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• Status Lights – Green LED indicates the system is okay, red LEDs indicate if the lid is open, a filter is needed, the
flow rate is below desired level, or the battery power is low.
• Mode Selection Knob – Rotary knob for selecting the operating mode and turning the unit off.
• Inlet Cap – Protect from water ingress and is designed as part of the omni-directional inlet.
3.2 CHECK STATUS BUTTON AND ASSOCIATED LEDS
The ACD-200 is equipped with five LEDs that communicate the status of the unit to
the user. The appropriate LEDs are illuminated when the Check Status button is
pushed. The red Check Lid LED is illuminated when the lid is open. The red Check
Filter LED is illuminated if there is not a filter cassette present in the sampler. The
Flowrate LED is illuminated when the internal mass flow sensor is measuring a flow
rate less than what has been selected. This may be due to an overloaded filter or a
blockage at the exhaust vents located on the bottom of the unit. The Battery LED is
illuminated when the battery needs to be recharged.
FIGURE
3.2
CHECK STATUS BUTTON
AND LED
s

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4 PROCEDURES
This chapter includes some of the basic procedures used while operating the Bobcat.
4.1 SETTING UP THE ACD-200 BOBCAT FOR COLLECTION
Select the desired area for sample collection. For each leg, push the locking push pin located on the leg hinge at the base
of the Bobcat as shown in Figure 4.1 and 4.2. It should be noted that the Bobcat cannot be operated with the legs in the
upright position since the exhaust vents are located on the bottom of the unit and the Inlet can’t be opened.
Unfold the leg until the push pin pops out and locks the leg into place as shown in Figure 4.3. Open the leg latches, as
shown in Figure 4.4, and telescope the legs out to the desired height.
Supporting the back of the unit with one hand, push the 10-Pin Connector (see Figure 4.5) on the Power and Trigger
Connector into the Power and Trigger Port on the front of the unit. It will automatically lock into place. Push the barrel
plug on the external power supply (see Figure 4.6) into the barrel jack on the Power and Trigger Connector. Plug one end
of the AC Power Cord (Figure 4.7) into the External Power Supply and plug the other end into an appropriate wall outlet.
FIGURE
4.1
LOCKING PUSH PIN LOC
ATION AT LEG HINGE
FIGURE
4.2
UNLOCKING AND UNFOLD
ING LEG
FIGURE
4.3
LEG PUSH PIN LOCKED
IN PLACE
FIGURE
4.4
OPENING LEG LATCH FO
R ADJUSTMENT

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4.2 OPTIONAL BATTERY OPERATION
The ACD-200 has a rechargeable battery for
operating the unit in the field. Instructions for
using the battery are as follows. The battery
door is located on the bottom of the unit.
The Battery Cover is removed by pinching the
retaining clips on both sides, as shown in
Figure 4.9, and pulling the cover straight out of
the bottom of the unit, as shown in Figure 4.10.
The battery is installed into the unit with the
battery socket towards the front of the unit as
shown in Figure 4.11. Reinstall the Battery Cover by pushing it into the
bottom of the unit until both clips click into place.
FIGURE
4.5
POWER
AND TRIGGER
CONNECTOR
FIGURE
4.6
EXTERNAL POWER SUPPL
Y
WITH BARREL PLUG
FIGURE
4.7
AC POWER CORD
FIGURE
4.8
BATTERY COVER ON THE
BOTTOM OF THE UNIT
FIGURE
4.10
REMOVING
BATTERY COVER
FIGURE
4.11
INSTALLING
BATTERY
Battery Socket
FIGURE
4.9
PINCHING CLIPS ON
BATTERY COVER

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4.3 INSTALLING THE FILTER
Open the ACD-200 by putting one hand on top of the Inlet and while grasping it, press the Release Button. The top will
open on its hinge. Insert the Filter into the top of the Collector as shown in Figure 4.12. The Filter housing is designed to
only fit in one way. Close the Inlet lid and hold it down to lock it in place, as shown in Figures 4.13 and 4.14.
A Trigger accessory is required to operate the Bobcat in the “Triggered” mode. The Trigger (Item # AC08078) is a 22ft
cabled switch available for models purchased after February 2018. For models purchased prior to February 2018, please
contact InnovaPrep for more information.
4.4
C
OLLECTING A
S
AMPLE
The unit is controlled with a single rotary switch
as shown in Figure
4
.15
.
The selections are Power OFF,
Triggered, Continuous, and three run modes:

Continuous mode

minute on, 1 minute off
1
—
sample collection at
Lpm
200
.

minutes on,
5
1
minutes off
5
—
sample collection at
Lpm
200
.

Extended Run
—
this selection samples for 1 minute on and
5
minute
s
off
with
sample
collec
tion
at 100 Lpm.
Five status lights are used to signal the system
’
s status and to alert the user
to errors. A check status push
-
button switch can be used to tu
rn the status
lights on
momentarily
so the
device is not readily seen in dark conditions
i.e. under “light discipline” conditions).
(
FIGURE
4.15
MODE SELECTION KNOB
FIGURE
4.12
INSTALLING
FILTER CASSETTE
FIGURE
4.13
CLOSING LID
FIGURE
4.14
RELEASE BUTTON

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4.5 RECOVERY OF THE SAMPLE
When the run is completed, recover the Filter sample by unlatching the Inlet, and pressing the Sample Cup into the top of
the Filter as shown in Figure 4.16 and pressing firmly until it clicks all the way around the cup. The Filter and Sample Cup
are then lifted out of the collector, flipped over, and capped with the Elutor as shown in Figure 4.17 (make sure the Filter
and Elutor are fully seated) then seal in a zip-type bag for transporting dry as shown in Figure 4.18. Alternatively, the Filter
can be eluted into the Sample Cup, as shown in Figure 4.19, while in the field then capped with the Sample Cup Lid.
4.6 ELUTING A FILTER SAMPLE
To extract the captured particles from the filter contained, follow the procedures below:
1. Place the Sample Cup, Filter, and Elutor assembly, as shown in Figure 4.19, on a
stable surface.
2. Firmly press the Elution Canister into the Elutor, also shown in Figure 4.19. The
elution foam is released from the Elution Canister through the Filter when the
canister valve is pressed down. Hold the canister down until all the foam is
released. The foam quickly collapses back to a liquid in the sample cup, making it
available for sample processing and analysis.
4.7 CLEANING THE SYSTEM
These procedures should be used when the instrument is placed in or removed from storage and for periodic cleaning.
This procedure is performed using the Bobcat Cleaning and Maintenance Kit (Part # AC00203) that is available for purchase
from InnovaPrep. Remove the sampler from the work area, hot zone, or contaminated area to an area where the cleaning
can be safely performed, if applicable, and disconnect the AC power converter and/or remove the battery.
Open one of the cleaning wipe packets contained in the Bobcat Cleaning and Maintenance Kit and use the enclosed
towelette to wipe down the exterior surface of the inlet as shown in Figure 4.20. Wet the foam tipped applicator with the
cleaning wipe and clean the inlet under the inlet cap as shown in Figure 4.21. Open the second cleaning wipe packet
contained in the Bobcat Cleaning and Maintenance Kit and use the enclosed towelette to wipe down the interior surfaces
of the sampler as shown in Figure 4.22.
FIGURE
4.16
SAMPLE CUP SNAPPED I
NTO
THE FILTER
FIGURE
4.17
ELUTOR
SNAPPED O
NTO
THE FILTER
FIGURE
4.18
ASSEMBLY IS BAGGED A
ND
READY
FOR TRANSPORT TO THE
LABORATORY
FIGURE
4.19
ELUTION OF FILTER

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4.8 BATTERY CHARGING
The battery charger consists of two parts, the battery cap and a “wall wart” style AC power adapter, permanently
connected by a flexible cable. The AC power adapter is supplied with a standard North American AC plug installed. Other
AC plugs are available for International use; contact InnovaPrep for more information. Plug the AC power adapter into any
available AC mains socket. The LED on the power supply will illuminate, and the battery cap indicator will indicate the
Stand-by state (see below). Make sure that the connector and contact pads on the battery are clean and free of foreign
matter, and push the battery cap all the way into the battery connector. The charge cycle for the attached battery will
start automatically.
Indicator Light State of Charging Process
Slow Blinking Yellow Stand-by – Prequalification
Fast Blinking Yellow Precharge
Steady Yellow Normal Charge
Steady Green Full Charge
Red Battery or Charger Fault
Note: Maximum storage temperature for the battery is 40°C and charging temperature maximum is 37°C
Please contact InnovaPrep or an official InnovaPrep distributor for technical assistance and troubleshooting.
132 East Main Street, Box 68, Drexel, MO 64742 Phone:
816-619-3375
[email protected] and www.innovaprep.com
Revision
Date
Author
1
November 12, 2013
P. Murowchick
2
February 24, 2015
P. Murowchick
3
August 8, 2017
A. Packingham
4
March 8, 2018
A. Packingham
5
April 20, 2018
D. Alburty
6
August 28, 2018
A. Packingham
7
September 11, 2018
A. Packingham
8
February 18, 2020
A. Packingham
FIGURE
4.20
CLEANING EXTERIOR
SU
RFACES
FIGURE
4.21
CLEANING INLET WITH
FOAM TIPPED APPLICAT
OR
FIGURE
4.22
CLEANING INTERIOR
SURFACES

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