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USER’S GUIDE
EN
PIPETMAN M
USER’S GUIDE
EN
PIPETMAN M
9GUIDELINES FOR GOOD PIPETTING
Aspirate and dispense
1) Fit new PIPETMAN DIAMOND Tip for the best results (refer
to chapter 18).
For single channel models, push the tip-holder into the tip
using a slight twisting motion to ensure a firm and airtight
seal.
Formultichannelmodels,PIPETMAN DIAMONDTipsarebest
fitted from the patented ROCKY RACK available only in our
TIPACKsandTOWERPACKs. ROCKYRACKisthedome-shaped
part of the pack that contains the tips. ROCKY RACK makes
it easy to securely fit the tips to a multichannel pipette,
ensuring an airtight seal on all channels without the need
to use undue pressure or to touch the tips.
2) Pre-rinse the tip. Pre-rinsing consists of aspirating the first
volume of liquid and then dispensing it back into the same
vessel (or to waste). Subsequent volumes that you pipette
will have levels of accuracy and precision within specifica-
tions. Some liquids (e.g. protein-containing solutions and
organic solvents) can leave a film of liquid on the inside the
wall of the tip; pre-rinsing the tip minimizes any errors that may be related to this phenomenon.
3) Hold the pipette vertically and immerse the tip in the
liquid (see page 13). Press the push-button to aspirate the
set volume of liquid. Wait a few seconds (time depends on
model, see page 13); then withdraw the pipette-tip from the
liquid. You may wipe any droplets away from the outside of
the tip using a medical wipe, however if you do so take care
to avoid touching the tip’s orifice.
4) Placethe endofthetip againsttheinside walloftherecipient
vessel (at an angle of 10° to 40°). Press the push-button. Wait
for at least a few seconds before releasing the push-button
to expel any residual liquid from the tip. While removing the
pipette draw the tip along the inside surface of the vessel.
5) Eject the tip by pressing firmly on the tip-ejector button.
General guidelines for good pipetting
a. Make sure that you fit new tips.
b. Eachnewtipshouldbepre-rinsedwith theliquid tobepipetted.
Immersion depth and wait time
Model Immersion depth Wait time
(mm) (seconds)
P10M 1 1
P20M 2-3 1
P100M 2-4 1
P200M 2-4 1
P300M 2-4 1
P1200M 2-4 2-3
P5000M 3-6 4-5
P10 mLM 5-7 4-5
P8x10M, P12x10M 1 1
P8x20M, P12x20M 2-3 1
P8x100M, P12x100M 2-4 1
P8x200M, P12x200M 2-3 1
P8x300M, P12x300M 2-3 1
P8x1200M, P12x1200M 2-4 2-3
c. When aspirating, keep the tip at a constant depth below the surface of the liquid (refer to table).
d. Change the tip before aspirating a different liquid, sample, or reagent.
e. Change the tip if a droplet remains at the end of the tip from the previous pipetting operation.
f. Liquid should never enter the tip holder. To prevent this:
• Never turn the pipette upside down,
•Never lay the pipette on its side when there is liquid in the tip(s),
g. The Gilson Stand Adapter (refer to chapter 2) is recommended for use with the CARROUSEL and
SINGLE Pipette Holder to store your PIPETMAN M pipette in the vertical position. Alternatively the
POWER CARROUSEL can be used to store and charge up to 5 PIPETMAN M pipettes.
h. When pipetting liquids with temperatures different to the ambient temperature, pre-rinse the tip
several times before use in order to reach equilibrium between the temperatures of the liquid and
the pipette’s dead-volume.
i. For volatile liquids you should saturate the dead-volume by aspirating and dispensing the liquid
repeatedly before aspirating the sample.
j. After pipetting acids or other corrosive liquids that emit vapors, clean the pipette, as described in
chapter 15.
k. The pipette can be used between + 4 °C and + 40 °C, but the
specifications may vary (refer to chapter 18).
l. Do not pipette liquids having temperatures above 50 °C or
below 4 °C.
10 PERSONALIZE YOUR PIPETMAN M
Tip-ejector extension for use with P10M
In order to eject D10 tips, tip-ejector extensions are supplied with P10M
pipettes.
The tip-ejector extension which is made of PVDF (polyvinylidene Fluoride),
is autoclavable.
To t a tip-ejector extension:
• Hold the pipette with the push-button upright.
• Hold the extension with the slot upright.
• Slide the extension over the tip-holder.
• Push the extension firmly onto the end of the tip-ejector until it clicks
into place (see opposite).
To remove a tip-ejector extension (see opposite):
• Hold the pipette in one hand and grip the extension with the other.
• Gentlytwistthe extension(eitherdirection) andpullitawayfromthe pipette.
Short tips Long tips
To fit To remove
Slot
Extreme temperatures
can aect accuracy and
precision!
