Kendro HERAcell 150 User manual

Sicherheitswerkbank HERAsafe HS 9/12/15/18
Valid: 07.2004 / 50075549 B
CO2incubator HERAcell® 150
with decontamination routine
Operating Instructions

CO2 Incubator HERAcell® 150 Operating Instructions
2
Copyright©
These operating instructions are protected by copyright. Rights resulting the-
reof, particularly reprint, photomechanical or digital postprocessing or reproduc-
tion, even in part, are only allowed with the written consent of Kendro Laboratory
Products GmbH.
This regulation does not apply to reproductions for in-plant use.
The contents of the operating instructions are subject to change without further
notice. For translations into foreign languages, the German version of these ope-
rating instructions is binding.
Trademarks
HERAcell® and Heraeus are registered trademarks of Kendro Laboratory Pro-
ducts GmbH. All other trademarks mentioned in the operating instructions are
the exclusive property of the respective manufacturers.
Kendro Laboratory Products GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Germany

CO2 Incubator HERAcell® 150 Operating Instructions
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Contens
1. General notes ........................................................................................ 6
1.1 General safety instructions .......................................................... 7
1.2 Warranty ..................................................................................... 7
1.3 Explanation of symbols ............................................................... 8
1.3.1 Symbols used in the operating instructions:.............................. 8
1.3.2 Symbols used in the quick referenc: ......................................... 9
1.3.3 Symbols on the device: .......................................................... 10
1.4 Intended purpose of the device ..................................................11
1.4.1 Correct use .............................................................................11
1.4.2 Incorrect use: ..........................................................................11
1.5 Standards and directives ........................................................... 12
1.6 Safety notes on gases ............................................................... 12
2.Delivery.................................................................................................... 15
2.1 Packaging ................................................................................. 15
2.2 Components standard equipment .............................................. 15
2.3 Acceptance inspection .............................................................. 16
3. Installation of the device ..................................................................... 17
3.1 Ambient conditions .................................................................... 17
3.2 Room ventilation ....................................................................... 17
3.3 Space requirements .................................................................. 18
3.4 Transport ................................................................................... 18
3.5 Stacking .................................................................................... 19
3.6 Retrofitting/Modifications ........................................................... 19
3.7 Tray........................................................................................... 19
4. Description of the device .................................................................... 20
4.1 Front view ................................................................................. 20
4.2 Rear view .................................................................................. 21
4.3 Safety devices........................................................................... 22
4.4 Work space atmosphere ............................................................ 22
4.5 ContraCon decontamination routine .......................................... 23
4.6 Sensor system .......................................................................... 24
4.7 Door switch ............................................................................... 24
4.8 Switchbox with supply interface ................................................. 25
4.9 Work space components ........................................................... 26
5. Start-up ................................................................................................ 29
5.1 Preparing the work space .......................................................... 29
5.2 Installing the shelf system ......................................................... 29
5.3 Gas connection ......................................................................... 31
5.3.1 Installing gas pressure hoses ................................................. 32
5.3.2 Installing devices without gas guard ....................................... 32
5.3.3 Connecting devices with gas guard ........................................ 33
5.4 Power supply connection........................................................... 35
5.5 RS 232 interface connection ..................................................... 36
5.6 Connecting the alarm contact .................................................... 38
6. Handling and control .......................................................................... 40
6.1 Power switch ............................................................................. 40
6.2 Operating panel ......................................................................... 40
6.3 Control self-test ......................................................................... 41

CO2 Incubator HERAcell® 150 Operating Instructions
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Contens
6.4 Setting the nominal temperature value ...................................... 42
6.5 Setting the O2 nominal value ..................................................... 42
6.6 Disabling the O2 control ............................................................. 43
6.7 Enabling the O2 control .............................................................. 44
6.8 Setting the CO2 nominal value ................................................... 45
6.9 Setting the high/low humidity ..................................................... 45
6.10 Activating the auto-start routine ................................................. 47
6.11 Reading failure codes................................................................ 49
6.12 Failure code list ......................................................................... 52
6.13 Gas guard failure display ........................................................... 53
6.14 Resetting the thermal protection ................................................ 54
7. Operation ............................................................................................. 55
7.1 Preparing the device ................................................................. 55
7.2 Starting operation ...................................................................... 56
8. Shut-down ........................................................................................... 57
8.1 Shutting the device down .......................................................... 57
9. Cleaning and disinfection ................................................................... 58
9.1 Decontamimation procedures .................................................... 58
9.2 Wipe/Spray disinfection ............................................................. 58
9.3 ContraCon decontamination routine .......................................... 62
9.4 Activating the ContraCon decontamination routine .................... 65
9.5 Cancelling the ContraCon decontamination routine ................... 65
10. Maintenance ........................................................................................ 66
10.1 Inspections and checks ............................................................. 66
10.2 Service intervals ........................................................................ 66
10.3 Preparing the temperature calibration ........................................ 67
10.4 Temperature calibration procedure ............................................ 68
10.5 Preparing the CO2 calibration .................................................... 69
10.6 CO2 calibration procedure ......................................................... 70
10.7 Replacing the sterile filters ........................................................ 71
10.8 Replacing the device fuses........................................................ 71
10.9 Replacing the door seal ............................................................. 72
11. Spare parts and accessories .............................................................. 73
11.1 List of spare parts and accessories............................................ 73
12. Technical data ..................................................................................... 75
13. Disposal ............................................................................................... 78
14. Principles of good microbiological proceedings1 ............................ 79
15. Device log ............................................................................................ 81
16. Certificate of decontamination ........................................................... 82

CO2 Incubator HERAcell® 150 Operating Instructions
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Figures
Fig. 1 Device dimensions and minimal distances................................. 18
Fig. 2 Lift points ................................................................................... 18
Fig. 3 Stacking .................................................................................... 19
Fig. 4 Tray installation ......................................................................... 19
Fig. 5 Front view ................................................................................. 20
Fig. 6 Rear view .................................................................................. 21
Fig. 7 CO2 and O2sensors................................................................... 24
Fig. 8 Door switch ............................................................................... 24
Fig. 9 Supply interfaces....................................................................... 25
Fig.10 Work space layout ..................................................................... 26
Fig.11 Water reservoir, gas humidification ............................................ 27
Fig.12 Rear panel openings ................................................................. 28
Fig.13 Support rail installation/removal ................................................. 29
Fig.14 Support hook/Insertion sheet installation ................................... 30
Fig.15 Center strut/gas humidification installation ................................. 30
Fig.16 Gas pressure hose installation .................................................. 32
Fig.17 CO2 connection, combined CO2-/O2-/N2- connection .................. 32
Fig.18 Switchbox with gas guard .......................................................... 33
Fig.19 CO2 connection with gas guard ................................................. 33
Fig.20 Combined CO2-/O2-/N2-connection gas guard ............................ 34
Fig.21 Power supply connection........................................................... 35
Fig.22 RS 232 interface connection ..................................................... 36
Fig.23 Example of a connection scheme for an external alarm system . 39
Fig.24 Power switch ............................................................................. 40
Fig.25 Operating panel functions .......................................................... 40
Fig.26 Blower wheel/Cover removal ..................................................... 61
Fig.27 ContraCon decontamination routine procedure.......................... 63
Fig.28 Temperature calibration ............................................................. 67
Fig.29 CO2 calibration .......................................................................... 69
Fig.30 Sterile filter replacement ............................................................ 71
Fig.31 Device fuse replacement ........................................................... 71
Fig.32 Door seal replacement .............................................................. 72
Fig.33 Gas consumption ...................................................................... 77

CO2 Incubator HERAcell® 150 Operating Instructions
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1. General notes
The following are the addresses of the international Kendro
Sales Organisations.
Postal address Germany
Kendro Laboratory Products GmbH
Robert-Bosch-Straße 1
D - 63505 Langenselbold
Anfragen aus Deutschland
Telefon
Vertrieb 0800 1 536376
Service 0800 1 112110
Fax
Vertrieb/Service 0800 1 112114
E-Mail [email protected]
Enquiries from Europe, Middle East
and Africa
Tel. + 49 (0) 6184 / 90-6940
Fax + 49 (0) 6184 / 90-7474
E-Mail [e-mail]
Postal address USA
Kendro Laboratory Products Inc.
275 Aiken Road
Asheville, NC 28804
USA
Enquiries from North America
Phone [phone]
Fax [phone]
E-Mail [e-mail]
Enquiries from Latin America
Phone [phone]
Fax [phone]
E-Mail [e-mail]
Enquiries from Asia Pacific
Phone [phone]
Fax [phone]
E-Mail [e-mail]

CO2 Incubator HERAcell® 150 Operating Instructions
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1. General notes
1.1 General safety instructions
These operating instructions describe the CO2 incubator HERAcell® 150.
The CO2 incubator has been manufactured in keeping with the latest techno-
logical developments and is operationally safe. However, the device may pre-
sent potential hazards, particularly if it is operated by inadequately trained
personnel or if it is not used in accordance with the intended purpose. There-
fore, the following must be observed to prevent accidents:
• The CO2 incubator must only be operated by trained and authorized personnel.
• For personnel operating this device, the operator must prepare written instruc-
tions in a reasonable form based on these operating instructions, the safe-
ty data sheets, the hygiene regulations and the applicable Technical
Guidelines, in particular:
• which decontamination measures are to be taken for the device and
for the accessories used,
• which safety measures are to be taken when gases and pressurized
gas containers are used,
• which measures are to be taken in case of an accident.
• Any repairs to the device must only be performed by adequately trained and
authorized expert personnel.
• The contents of the operating instructions are subject to change without further
notice.
• Concerning translations into foreign languages, the German version of these
operating instructions is binding.
• Keep these operating instructions in the vicinity of the device so that safe-
ty instructions and important information are always accessible.
• Should you encounter problems that are not mentioned in these operating
instructions, please contact Kendro Laboratory Products GmbH immediately
for your own safety.
1.2 Warranty
Kendro Laboratory Products warrant the operational safety and the operativeness
of the CO2 incubator HERAcell®150 only under the condition that:
• the device is operated and serviced exclusively in accordance with its intended
purpose and as described in these operating instructions,
• the device is not modified,
• only original spare parts and accessories that have been approved by Kendro
Laboratory Products are used,
• inspections and maintenance works are carried out at the specified intervals.
The warranty is valid from the date of delivery of the device to the operator.

CO2 Incubator HERAcell® 150 Operating Instructions
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1. General notes
1.3 Explanation of symbols
1.3.1 Symbols used in the operating instructions:
WARNING!
is used if non-observance may cause serious
or even lethal injuries.
CAUTION!
is used if non-observance may cause medium
to minor injuries or damage.
NOTE
is used for applicational hints and useful
information.
Wear safety gloves!
Wear safety goggles!
Harmful liquids!
Electric shock!
Hot surfaces!
Fire hazard!

CO2 Incubator HERAcell® 150 Operating Instructions
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1. General notes
1.3.2 Symbols used in the quick referenc:
Operational steps to be performed at the device
Display state of device indicators
Turn device on
Turn device off
Open device doors
Leave device doors open for at least 30 seconds
Check humidification system water level, refill as necessary,
level between 1.2 l (min.) and 3.0 l (max.)
Refill 300 ml water for ContraCon disinfection routine
Initiate auto-start, keep key depressed for at least 5 seconds
Initiate ContraCon decontamination routine, keep key
depressed for approx 5 seconds
Set nominal values for temperature, CO2and O2.

CO2 Incubator HERAcell® 150 Operating Instructions
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1. General notes
Clean device work space
Close device doors
Remove samples and water from device
Charge device
Note in operating instructions, page ...
1.3.3 Symbols on the device:
CE symbol
VDE - safety-tested
Test certificate for USA/Canada
Observe operating instructions!

CO2 Incubator HERAcell® 150 Operating Instructions
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1. General notes
1.4 Intended purpose of the device
1.4.1 Correct use
The CO2 incubator HERAcell® 150 is a laboratory device for preparing and cul-
tivating cell and tissue cultures. The device allows the simulation of the speci-
al physiological ambient conditions for these cultures due to the exact control
of:
• temperature,
• CO2content,
• O2 content,
• and the setting of an increased relative humidity.
The HERAcell® 150 has been designed for installation and operation in the fol-
lowing fields of application:
• Laboratories for cytobiological and biotechnological experiments of safety
levels L1, L2, and L3.
• Medical-microbiological laboratories in accordance with DIN 58 956.
• Laboratories in the central area of clinics and hospitals.
The gases required for the incubator (CO2 and/or N2/ O2) are supplied to the
device from a separate gas supply system, either from gas cylinders or from a
central pressurized gas container.
The layout of the gas supply system must ensure that the operating pressure
of the gas supply lines can be set to a range between 0.8 bar (min.) to 1 bar (max.)
and that the pressure cannot be changed.
Depending on the capability of the gas supply system, several devices may be
connected in series.
The CO2 incubator is suited for continuous operation.
1.4.2 Incorrect use:
Do not use cell or tissue cultures in the device that are not in accordance with
the regulations of safety levels L1, L2, and L3.
Do not use tissues, substances or liquids that:
• are easily ignitible or explosive,
• release vapors that form combustible or explosive mixtures when exposed
to air,
• release poisons.

CO2 Incubator HERAcell® 150 Operating Instructions
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1. General notes
1.5 Standards and directives
The device is in accordance with the following standards and guidelines:
• DIN EN 61010
• Low Voltage Guideline 73/23 EWG
• EMC Guideline 89/336 EWG
• UVV VBG 20
• DIN 12880 Part 1/11.78
The following safety regulations must be observed if the device is operated within
the territory of the Federal Republic of Germany:
• ZH 1/10
• ZH 1/119
• ZH 1/342
• ZH 1/343
• ZH 1/598
• TRG 280
• EC Official Gazette, L 374
• Safety data sheets of the gas supplier relevant to the particular characteri-
stics of CO2, O2, and N2.
• Principles of good microbiological proceedings, notice of the trade association
of the German chemical industry.
For other countries, the applicable national regulations are binding.
1.6 Safety notes on gases
Carbon dioxide (CO2):
As CO2 is rated as a harmful gas, certain safety instructions must be observed
when the CO2 incubator is started up and when the device is operated.
NOTE – Personnel instruction
Personnel operating devices with CO2 supp-
ly must be instructed about the particularities
in the handling of CO2 before starting their
work:
• Correct operation of pressurized gas con-
tainers and gas supply systems
(e.g. TRG 280),
• Obligation to report damages and shortco-
mings in CO2 supply lines,
• Measures to be taken in case of accidents
or failures.
These instructions must be repeated in appropriate intervals and must comprise
the particular operating instructions of the gas supplier.

CO2 Incubator HERAcell® 150 Operating Instructions
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1. General notes
WARNING - Suffocation hazard!
CO2 released in large amounts into the room
atmosphere may cause suffocation.
If CO2 is released, initiate safety measures
immediately!
• Leave the room immediately and do not al-
low others to enter the room!
• Inform security service or fire department!
Oxygen (O2):
O2 is a gas that promotes combustion and may explode in combination with
grease-containing materials.
WARNING – Oxygen explosion!
O2 may explode in combination with oils, grea-
ses, and lubricants. If highly compressed oxy-
gen comes in contact with grease- or oilcon-
taining substances, the mixture may explode!
• For cleaning these device components, use
only oil- and grease-free lubricants.
• Keep all connections and components of
the oxygen system free from substances
that contain oil, grease, or lubricant!
CAUTION – Fire hazard!
Released oxygen promotes combustion. Do
not use open flames in the vicinity of oxygen-
operated systems!
• Do not smoke in the vicinity of oxygen
systems.
• Do not expose the components of an oxy-
gen system to excessive heat.

CO2 Incubator HERAcell® 150 Operating Instructions
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1. General notes
Nitrogen (N2):
Nitrogen mixes easily with air. High concentrations of nitrogen reduce the oxygen
content in the air.
CAUTION – Suffocation hazard!
N2 released in large amounts into the room
atmosphere may cause suffocation due to
oxygen deficiency.
If N2 is released, initiate safety measures im-
mediately!!
• Leave the room immediately and do not
allow others to enter the room!
• Inform security service or fire department!
NOTE – Installation work
Any work to supply lines and pressurized gas
containers, cylinders or containers used for
storing N2, CO2 or O2 must only be carried out
by expert personnel using the appropriate
tools.

CO2 Incubator HERAcell® 150 Operating Instructions
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2. Delivery
2.1 Packaging
The CO2 incubator HERAcell® 150 is delivered in a stable packaging box. All
packaging materials can be separated and are reusable:
Packaging materials
• Packaging carton Recycled paper
• Foam elements Styrofoam (CFC-free)
• Pallet Untreated wood
• Packaging film Polyethylene
• Packaging ribbons Polypropylene
2.2 Components standard equipment
Quantity of the delivered
components
CO2 or CO2/O2 incubator with
solid glass door and with
continuous shelves
(standard version)
CO2- or CO2/O2-Incubator with
3-door gas tight screen and with
continuous shelves
(optional)
Shelf 3 3
Support rail for shelf 4 4
Shelf supports for shelf 6 6
Tray 1 1
Insert for pressure compensation
opening 1 1
Plug for pipe channel 1 1
Power supply cable 1 1
Connector, potential-free contact 1 1
Spare caps, set 1 1
CO2 connecting hose set 1 1
Immersion water pump 1 1
Open-end wrench,
24 mm 1 1
Allen wrench 2 mm for blower
wheel 1 1
Allen wrench 3 mm for blower
wheel cover 1 1
Operating instructions 1 1
Quick reference 2 2
O2connecting hose set 1 1
O2sensor with set for gas
humidification11 1
Additional equipment
1 The gas humidification set is packed in a separate carton and placed in the sample chamber during transport.

CO2 Incubator HERAcell® 150 Operating Instructions
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2. Delivery
2.3 Acceptance inspection
After the device has been delivered, check the delivery immediately for:
• completeness,
• possible damages.
If damages are detected or if components are missing, please contact the
carrier and Kendro Laboratory Products immediately.

CO2 Incubator HERAcell® 150 Operating Instructions
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3. Installation of the device
3.1 Ambient conditions
The device must only be operated at locations that meet the particular ambient
conditions listed below.
Requirements:
• Draft-free and dry location.
• The minimal distance to adjacent surfaces must be observed on all sides (see
Section 3.3.).
• The operating room must be equipped with an appropriate room ventilation.
• Solid, level, fire-proof surface.
• Vibration-proof substructure (floor stand, lab table) capable of bearing the
weight of the device and of accessories (particularly if several devices are
stacked).
• To ensure a constant incubation temperature of 37° C, the ambient tempe-
rature must be within a range of +18° C to +33° C.
• Relative humidity up to 80 % (max.).
• No direct exposure to sunlight.
• Devices that produce excessive heat are not allowed near the location of the
HERAcell® 150.
3.2 Room ventilation
When CO2/O2/N2 is supplied, the work space of the incubator is slightly pres-
surized. The pressure is released through the pressure compensation opening
into the operating room.
As the pressure compensation and any opening of the glass door/gas tight screen
during the operation of the device will release very small quantities of CO2/
O2/N2 into the operating room, the room ventilation must be capable of carrying
the released gas safely off into the open.
In addition, heat dissipating from the device during permanent operation may
cause a change in the room climate.
• Therefore, the HERAcell® 150 must only be installed in rooms with sufficient
ventilation.
• Do not install the device in room recesses without ventilation.
• The room ventilation should be a technical ventilation that is in accordance
with the requirements of ZH 1/119 (Guidelines for laboritories) or some other
suited ventilation system with appropriate capacity.

CO2 Incubator HERAcell® 150 Operating Instructions
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3. Installation of the device
3.3 Space requirements
Fig. 1: When installing the device, make sure that the instal-
lation and supply connections are freely accessible.
The control box at the rear panel of the device may serve as
a spacer to adjacent objects. The side distances given are mi-
nimal distances.
To protect the CO2 incubator against contamination, use a floor
stand even if the device is installed near the floor. The height
of the floor stand should not fall below 200 mm.
Several floor stands and carriers are available as options from
Kendro (part numbers see Section 11.1, “Spare parts and
accessories”).
NOTE – Accessibility of the devices
To ensure the accessibility for care and mainte-
nance works, keep larger side and rear
distances.
3.4 Transport
Fig. 2: For the transport do not lift the device using the doors
or components attached to the device (e.g. control box on rear
panel) as lift points.
NOTE – Lift points
Lift the device only using the lift points shown
in Fig. 2.
Fig. 1
Device dimensions and
minimal distances in mm,
F = Front
Fig. 2
Lift points

CO2 Incubator HERAcell® 150 Operating Instructions
19
Fig. 3
Stacking
Fig. 4
Tray installation
3. Installation of the device
3.5 Stacking
Fig. 3: Two HERAcell® 150 devices can be stacked on top of
each other. The upper device is secured to the lower device
by inserting the device stands [1] into the stacking elements
[2] on the device ceiling.
If the devices are placed onto mobile racks, ensure that the
rollers [3] are secured by an arresting device during operati-
on. For reasons of stability, the rollers should be oriented to
the front.
NOTE – Transporting stacked devices
The stacking elements are anot connecting
elements. Therefore, the transport of stacked
devices on sloped surfaces is not allowed.
3.6 Retrofitting/Modifications
The outer door and the glass door can be equipped with left
or right side fasteners. The door fastening can also be reversed
later.
The standard version can also be retrofitted with a three-ele-
ment split gas bezel by replacing the glass door with the gas
bezel.
NOTE – Modifications
Retrofittings and modifications must only be
performed by the Technical Service of Kendro
Laboratory Products.
3.7 Tray
Fig. 4: After the device has been installed, the tray is inserted
between the two guide straps below the bottom of the device.
1. Open the outer door of the device all the way to the stop.
2. Fold the guide straps [1] all the way down.
3. Position tray [4] with vertical edge [2] below cable guide [3].
The quick reference [5] faces the front of the device.
4. Lift tray slightly and tilt it downward.
5. Push tray in until resistance is observed, then push tray in
all the way to the stop by pushing onto guide straps.
4321
5
4

CO2 Incubator HERAcell® 150 Operating Instructions
20
Fig. 5
Front view
4.1 Front view
[1] Stacking elements
[2] Plug caps
[3] Glass door
[4] Measuring cell with blower wheel and sensors
[5] Door switch
[6] Oxygen sensor (optional)
[7] Pressure compensation opening with insert
[8] Measuring opening
[9] Outer door
[10] Outer door seal, replaceable
[11] Foot, height-adjustable
[12] Gas humidification (optional)
[13] Water level sensor
[14] Nameplate
[15] Tray, extractable
[16] Power switch
[17] Support rail
[18] Shelf
[19] Latch, glass door
[20] Support hook for shelf
[21] Access port with plug
[22] Glass door seal, replaceable
4. Description of the device