Kendro Heraeus Multifuge 1 S User manual

Multifuge®1 S / 1 S-R Instruction Manual

How to use this manual
Use this manual to get acquainted with your centri-
fuge and its accessories.
This manual helps you to avoid inappropriate han-
dling. Make sure to keep it close to the centrifuge.
A manual that is not kept handy cannot provide
protection against improper handling and thus
against damage to persons and objects.
The manual comprises chapters on
• Safety regulations
• Instrument description
• Transportation and hook-up
• Rotor program and accessories
• Use of the centrifuge
• Maintenance and care
• Troubleshooting
• Technical data
• Index
Please fold out
Overleaf you will find a graphic
representation of the control panel
with a survey of the most important
functions

speed/RCF
Quick run
run time
open lid
stop
start
The mains switch is located on the right-hand
side panel.
Pressed up = ON I
Pressed down = OFF 0
(only for instruments with refrigeration unit)
temperature
program selection
Bucket selection
rpm/RCF
switch key
display "set" keys
"set" keys
pre-temperature-regulation
Before switching on the centrifuge
please read this manual
deceleration
profiles
acceleration
profiles

Control panel of the Multifuge®1 S / 1 S-R
Display panels
Program selection
key 1 - 4 : store or recall programs.
acceleration profiles (1= slowest ...... 9= fastest)
permanent display: acceleration profile last set 1- 9
Deceleration profiles (1= slowest ...... 9= fastest)
permanent display: deceleration profile last set 1- 9
Speed / RCF
run: current value of speed or RCF after activation of
switch key
end: "End"
lid open: "OPEN"
"Lift Lid" (if lid is not automatically lifted off)
(before start)
lid closed: "0" with flashing point
(rotor not yet identified)
error code: will flash in display
Run time
time selection: - remaining run time to 0
continuous operation (hld):
- run time passed (in hours, minutes)
quick run: - run time passed (as long as button is held;
in minutes and seconds)
Temperature*
run: current sample temperature in °C
(in temperature equilibrium)
* only for instruments with refrigeration unit
Keys
start : normal start of the centrifuge
stop : manual stop of a run
open lid: open lid
(possible only with the instrument switched on)
quick run: short-term operation of the centrifuge as long as
key remains pressed
rpm/RCF
switch: switching between rpm and RCF display
bucket set.: Setting of the bucket number
pretemp : Pre-temp-function*
"set" keys: stepwise increase/decrease of setpoint values
Short pressing of any of the "set" keys: switch from current to preset
value, signaled by flashing display.
Error codes (troubleshooting see chapter "Troubleshooting"):
E-19: Unpermissible rotor
E-20: Rotor not identified
E-29: Motor or rotor blocked
E-31: Overtemperature in motor
E-32: Overtemperature in electronic
E-33: Excessive pressure in the refrigeration unit
E-34: Overvoltage
E-35: Overcurrent
Lift Lid : Smoothly lift the lid
rotor: set speed higher than permissible speed of
the rotor
bAL : imbalance

Contents
1
Contents
For your safety............................................ 3
Proper use................................................................ 3
Improper use ............................................................ 3
Centrifuging hazardous materials ............................ 3
Handling the centrifuge ............................................ 4
Conformity to current standards............................... 5
Safety instructions in this manual ............................ 5
The Multifuge®1 S / 1 S-R.......................... 7
Description ............................................................... 7
Safety systems......................................................... 8
Parts supplied .......................................................... 8
Function and features .............................................. 9
Before use................................................. 13
Centrifuge transport and installation ...................... 13
Proper location ....................................................... 13
Main connection ..................................................... 14
Rotors and accessories ........................... 15
Rotors for the Multifuge®1 S.................................. 16
Rotors for the Multifuge®1 S-R.............................. 20
Handling the rotor................................................... 29
Aerosol-tight operation ....................................... 32
Checking of aerosol-tight bio-containment......... 36
Operation .................................................. 37
Switching on the centrifuge .................................... 37
Actuating the lid...................................................... 37
Opening the lid ................................................... 37
Closing the lid ..................................................... 37
Installing the rotor................................................... 38
Loading the rotor .................................................... 39
Maximum loading ............................................... 39
Filling the centrifuge tubes ................................. 40
Maximum permissible load difference ................ 40
Inserting the centrifuge tubes ............................. 41
Entering parameters............................................... 43
Deceleration curves ............................................... 43
Switching from speed to RCF display .................... 43
Bucket selection for swinging bucket rotors........... 43
Selecting speed...................................................... 44
Entering the RCF value.......................................... 44
More about the RCF value ................................. 45
Selecting run time .................................................. 45
Run time selection .............................................. 45
Continuous operation ......................................... 46
Extended time mode........................................... 46
Selecting the temperature ...................................... 47
Pretemp function .................................................... 47
Starting the centrifuge ............................................ 48

Contents
2
Imbalance display................................................... 48
Changing the settings during the run ..................... 48
Stopping the centrifuge .......................................... 49
Stopping with preset run time............................. 49
Stopping with continuous operation ................... 49
Temperature control during standby ...................... 49
Working with programs........................................... 50
Program display.................................................. 50
Entering/changing a program ............................. 50
Centrifuging with a program ............................... 50
”Quick Run” ............................................................ 51
Removing the rotor................................................. 51
Audible alarm ......................................................... 52
Turning off the centrifuge ....................................... 52
Maintenance and care .............................. 53
Maintenance to be performed by the customer ..... 53
Cleaning.............................................................. 53
Disinfection ......................................................... 54
Decontamination................................................. 56
Autoclaving ......................................................... 56
The KENDRO service offer .................................... 57
Warranty conditions................................................ 57
Troubleshooting ....................................... 59
Emergency lid release............................................ 59
Error troubleshooting.............................................. 61
Contacting Kendro Service..................................... 70
Technical Data .......................................... 71
Electrical connections / fuses ................................. 73
Appendix ................................................... 75
Acceleration and deceleration profiles ................... 75
Speed/RCF diagrams............................................. 87
Index .......................................................... 93

For your safety
3
For your safety
Heraeus centrifuges are manufactured according to
current technical standards and regulations. Nonethe-
less, centrifuges may pose danger to individuals and
surrounding if
• they are not used as designed
• they are operated by untrained personnel
• their design is improperly changed
• the safety instructions are not followed
Therefore, personnel involved with operation and
maintenance of the centrifuge must read and follow
the safety instructions.
In addition, the pertinent regulations for prevention of
accidents must be strictly followed.
This manual is an integral part of the centri-
fuge assembly and must be kept close at
hand at all times.
When damages to the power cord or at
casing are noticed the centrifuge must
to be set out of operation!
Proper use
The centrifuge is designed to separate liquid-
suspended materials having different densities and
particle size, respectively (maximum sample density is
1.2 g/cm³ {ml} at maximum speed).
Improper use
During a run, a safety zone of 30 cm around the centri-
fuge must be maintained where neither persons nor
hazardous materials may be present.
The centrifuge may cause harm to its user or other
persons or may damage goods if safety measures are
not followed:
Centrifuging hazardous materials
• The centrifuge is neither made inert, nor is it explo-
sion-proof. Therefore never use the centrifuge in an
explosion-prone environment.
• Do not centrifuge explosive or flammable sub-
stances. The same holds for substances prone to
react violently with each other.

For your safety
4
• Do not centrifuge toxic or radioactive substances or
pathogenic microorganisms without suitable safety
systems.
If microbiological samples of risk group II (according
to "Laboratory Bio-safety Manual" of WHO) are be-
ing centrifuged, aerosol-tight bio-seals have to be
used.
For materials in a higher risk group, more than one
precaution is required.
• Should toxins or pathogenic substances enter the
centrifuge or its parts, you must perform appropriate
procedures for disinfection (see "Maintenance and
care – Disinfection").
• Strongly corrosive substances that may cause
damage to materials and reduce the mechanical
strength of the rotor may be centrifuged only inside
protective tubes.
Handling the centrifuge
• Use only original accessories for the centrifuge. The
only exceptions are common glass or plastic centri-
fuge tubes if they are approved for the rotor speed
and RCF values.
• Never use the centrifuge unless the rotor is properly
installed.
• You may use the centrifuge only with a properly
loaded rotor. You must not overload the rotor.
• Strictly follow the rules and regulations for cleaning
and disinfection
• If the rotor or the rotor lid shows signs of corrosion
or wear, you must stop using it.
• Never open the lid manually if the rotor is still turn-
ing.
• You may use the emergency lid release only in
case of emergency, e.g. during an interruption of
power supply
(see chapter "Troubleshooting").
• Never use the centrifuge with an opened lid.
• Never use the centrifuge if the front panel has been
partially or totally removed.
• Changes in mechanical or electrical components of
the centrifuge may only be carried out by individuals
authorized by Kendro Laboratory Products.

For your safety
5
Conformity to current standards
Heraeus centrifuges are manufactured and tested
according to the following standards and regulations:
- For all voltages
• IEC 61010
- For 120 V only
•
- For 230 V only
•
Please get acquainted with the details of the test stan-
dards from the technical data.
Safety instructions in this manual
This symbol denotes potential hazards to
persons.
This symbol denotes potential damage to
the centrifuge or parts in its immediate
surroundings.
General comments are marked with this
symbol.
In addition, you are asked to adhere to the pertinent
regulations, in Germany
• Regulations for prevention of accidents BGV A2
• Regulations for prevention of accidents VBG 5
• Regulations for prevention of accidents VBG 7z
- with cooled devices additionally
• Regulations for prevention of accidents BGV D4

For your safety
6
Notes

Description of the Multifuge®
7
The Multifuge®1 S / 1 S-R
The figure below shows a Multifuge®1 S-R with an
opened lid and a swinging bucket rotor installed.
Description
The Multifuge®1 S-R is a general-purpose tabletop
centrifuge for biotechnological and pharmaceutical
research that moves high capacity centrifugation onto
the fast track. It spins more tubes at higher RCFs more
rapidly than competitive instruments and can process
nominally 1.6 liters of sample in a single run. There are
various rotors available that can achieve high RCFs
and accommodate a wide range of accessories for all
common tube types, micro titer and deep well plates.
The user-friendly "EASYset" control panel permits easy
selection of speed, RCF value, run time and run profile
(acceleration and deceleration), as well as temperature
of the Multifuge®1 S-R. You can switch from speed to
RCF display and vice versa, with a touch of a button
and even during a run.

Description of the Multifuge®
8
Safety systems
The Multifuge®is equipped with a number of safety
systems:
• Housing and lid are constructed of 6 mm steel.
• Lid with window
• Lid lock with safety check
You can only open the centrifuge lid when the
power is turned on and the rotor has come to a
stop. You can only start the centrifuge if the lid is
properly locked.
• Automatic rotor identification
• Electronic imbalance detection as a function of rotor
(SMARTspinTM)
Do not tamper with the safety
systems!
Parts supplied
Accessories supplied with the centrifuge are:
− power cord
− a special wrench for securing the rotor
(As seen in the picture)
− condensed operating instructions
− corrosion protective oil
The printed documents consist of the delivery notes
and this Manual.

Description of the Multifuge®
9
Function and features
Basic unit/ function Description / feature
Cabinet / frame Galvanized steel
Chamber Stainless steel * (Multifuge 1S: coated steel)
Drive Brushless induction drive
Key pad and display Key pad and display elements covered by an easy-care continuous surface
Control Microprocessor driven by "EASYset"
Main memory Recalls last run parameters
Program memory Data are stored until new values are entered.
Advanced features RCF-programming, quick run, pre-temp *, temperature control during standby
Acceleration and deceleration pro-
files
9 acceleration and 9 deceleration profiles
Rotor identification Automatic
SMARTspinTM
imbalance detection system
Electronic, effective as a function of rotor and speed
Soft touch lid lock Motor assisted lid locking
(* only with refrigeration unit)

Description of the Multifuge®
10
The EASYset user interface
Function Feature
Program memory keys Freely programmable
Acceleration / deceleration profile 1 = slowest, ... 9 = fastest acceleration / deceleration curve
Setting speed by rpm Adjustable from 300 rpm to 15 000 rpm, in 10 rpm increments
RCF selection Upon actuation of RCF switch , the RCF value can then be entered
Time selection Adjustable in minutes from 1 min to 9 h 59 min,
or extended time mode from 1 min to 99 h,
”hld"-mode: continuous operation
Run time display in "quick run" mode Between 1 s and 60 s in seconds´ steps, above in minutes´ steps
Setting temperature * Adjustable from -9°C to +40°C, in one degree increments
End of centrifugation The speed display will read "end"
(* only with refrigeration unit)

Description of the Multifuge®
11
Function Feature
Lid opening Automatic unlocking via ”open lid" key ( )
(unlocking in case of power failure: see chapter ”Troubleshooting")
Start Start key ( )
Stop Stop key ( )
"Quick Run" mode Pressing the "quick run" key ( ) activates maximum acceleration up to the
maximum permissible speed of rotor; upon key release the centrifuge stops with
maximum deceleration power.
Diagnostic messages
• Alternating display ”Rotor"/maximum speed or RCF
(speed selected exceeds max. speed of the rotor)
• Lid has not been lifted off the lock during opening:
display ”lift lid" (manual lifting of lid is required)
• General instrument malfunction
(error messages with ERROR codes, see "Troubleshooting")

Description of the Multifuge®
12
Notes

Before use
13
Before use
Centrifuge transport and installation
After opening the box remove the protective materials.
When transporting the centrifuge,
consider its weight (see "Technical
Data"); always grab it on both sides
ensuring that enough helpers are
around.
Do not lift on the front panel!
The centrifuge can be damaged by
jolting during the transport!
Transport the centrifuge only in the
upright position using proper con-
tainment and secure it properly.
Handle the centrifuge carefully.
Proper location
The centrifuge may only be used indoors. Its location
must meet the following criteria:
• A safety zone of at least 30 cm (12 inches) around
the centrifuge must be maintained. Hazardous ma-
terials must not be stored beside the centrifuge dur-
ing its use.
• The laboratory bench or centrifuge trolley must be
stable and resonance-free. A good support is pro-
vided by a laboratory bench or a centrifuge cart with
lockable casters.
• To ensure sufficient air circulation, a minimum dis-
tance from the wall of 10 cm (4 inches) at the back
and of 15 cm (6 inches) on each side must be kept.

Before use
14
• The centrifuge must be protected from heat and
direct sunlight.
• The location must be well ventilated at all times.
UV rays reduce the durability of
plastics.
Protect the centrifuge, rotors and
plastic accessories from direct
sunlight.
Main connection
Connect the centrifuge only to a grounded main power
supply. Make sure that the power cord is compatible
with the valid safety regulations and that your main
voltage and frequency correspond to the specifications
on the instrument label.
Make sure that the centrifuge is switched off (on the
right-hand side of front panel) before connecting
electrical wire to main power supply.
RS232 interface
specifications
Main connection

Rotors and accessories
15
Rotors and accessories
A rotor is not included as part of a Multifuge®centri-
fuge.
A large variety of rotors are available as accessories.
In addition, there are adapters and reduction sleeves
for a variety of commercially available tubes and
bottles.
For a complete list of available tubes, bottles, adapt-
ers and accessories, please refer to our current sales
documentation.
For more information you can visit our web site at
http://www.Kendro.com
Multifuge®1 S (air cooled)
During centrifugation, heat is generated by air friction
between the rapidly spinning rotor and the air inside
the rotor chamber. The continuous air flow in the air
cooled centrifuge is restricting the temperature rise of
the samples. The equilibrium temperature rise de-
pends on the ambient temperature, the type of rotor,
the speed, the duration and the number of runs.
Use caution when touching rotors as
they may be hot after long runs at
high speed.

Rotors and accessories
16
Rotors for the Multifuge®1 S
Table 1: Rotors for Multifuge®1 S Differences of 120V instruments are shown in parentheses
Rotor designation Swinging Bucket Rotor TTH 400
75002000
With bucket
Order no.
Rectangular bucket
150 ml
75002001
Round bucket
400 ml
75002002
Maximum permissible load [ g ] 4 x 540 4 x 570
Maximum speed nmax [ rpm ] 4700 ( 4500 ) 4700
Maximum RCF value at nmax 4618 ( 4234 ) 4618
Radius (max.) [ cm ] 18.7 18.7
Acceleration / deceleration time [ s ] 40 / 35 ( 50 / 40 ) 40 / 35 ( 55 / 40 )
Heating of samples at nmax [ °C ]
relative to room temperature 23°C,
run time 1 hour
12.0 ( 14.0 ) 13.0
Aerosol-tight * Yes (with cap 75002003) Yes (with cap 75002004)
Autoclavable 121°C 121°C
* Tested and approved by CAMR, Porton-Down, UK