Kendro SORVALL Biofuge primo User manual

SORVALL®
Biofuge primo
OPERATING
INSTRUCTIONS
PN 00000-0

How to use this manual
Use this manual to get acquainted with your centri-
fuge and its accessories.
The manual helps you to avoid inappropriate han-
dling. Make sure to keep it always close to the centri-
fuge.
A manual that is not kept handy cannot provide
protection against improper handling and thus
against damage to persons and objects.
The manual comprises chapters on
• Safety regulations
• Instrument description
• Rotor program and accessories
• Transportation and hook-up
• Use of the centrifuge
• Maintenance and care
• Troubleshooting
• Technical data
• Index
Please fold out
Overleaf you will find a graphic
representation of the control panel
with a survey of the most important
functions

back panel:
socket for mains cable
mains switch
rpm/RCF
display
switch
"set" keys
quick run
open lid
stop
start
speed/RCF run time
braking profiles

Control panel of the Biofuge primo
Display
Braking profiles
Continuous
display: braking profile last used, 1 - 9.
2 - 9 = max. acceleration and various
braking profiles
( 2 [weak] to 9 [strong] )
1 = slow acceleration and
braking curve 2
Speed/RCF
During run: current rpm or RCF (after actuation of
display switch)
End of run: "End"
Lid open: "OPEN"
Error message: alternating display (if relevant)
Run time
During run: - remaining run time to 0
Continuous: - current run time
operation (hLd) (in hours and minutes)
"Quick run" : - run time passed
(in minutes and seconds)
Keys
Start : normal start
Stop: manual stop
Open lid: open lid (possible only with the instru-
ment switched on)
Quick run: short-term operation of the centrifuge as
long as key remains pressed
rpm/RCF
switch: Switching between rpm and RCF dis-
play.
"Set" keys: stepwise increase/decrease of preset
values, accelerated change when
pressed permanently
Short pressing of any of the "set" keys: switch from
current to preset value, signalled by flashing display.
Error codes (troubleshooting see chapter
"Troubleshooting"):
E-00: motor blockage
E-03 speed measurement
E-08: overvoltage
E-14: no rotor or rotor identification impossible
E-17: lid does not open
rotor: set speed higher than permissible speed
of the rotor
bAL: imbalance
Lid: lid turned loose or opened during run;
drive overheated
OPEN:with lid closed: safety circuit triggered (drive
overheated)

Contents
1
Contents
For your safety............................................ 3
Proper use................................................................ 3
Improper use ............................................................ 3
Centrifuging hazardous materials ............................ 3
Handling the centrifuge ............................................ 4
Conformity to current standards............................... 5
Safety instructions in this manual ............................ 5
The Biofuge primo...................................... 7
Safety systems......................................................... 7
Properties................................................................. 9
Items delivered......................................................... 9
Functions and features........................................... 10
The "Easycontrol" user interface............................ 11
Before use................................................. 13
Transport and installation....................................... 13
Proper location ....................................................... 13
Mains connection ................................................... 14
Accessories .............................................. 15
Rotor program ........................................................ 16
Adapters................................................................. 18
Handling rotors....................................................... 21
Swinging Bucket Rotors ..................................... 21
Lifetime of the rotor ............................................ 21
Permissible rotor temperature ............................ 21
Highconic®- rotor ................................................ 22
Drum rotor .......................................................... 22
Aerosol-tight operation ........................................... 23
Mikroliter rotor 75007587 and PCR-rotor
75007569............................................................ 24
Loading for aerosol-tight operation..................... 24
Checking of aerosol-tight bio-containment ......... 26
Operation .................................................. 27
Switching on the centrifuge .................................... 27
Opening the............................................................ 27
Closing the lid ..................................................... 27
Inserting the rotor ................................................... 28
Loading the rotor .................................................... 30
Maximum loading ............................................... 30
Filling the centrifuge tubes ................................. 30
Placing the tubes in the rotor.............................. 31
Entering parameters............................................... 32
Braking curves........................................................ 32
Switching from speed to RCF display and vice versa
............................................................................... 32
Selecting the speed................................................ 32
Entering the RCF value.......................................... 33
Concerning the RCF value ................................. 33

For your safety
2
Selecting the run time............................................. 34
Preselected run time........................................... 34
Continuous operation ......................................... 34
Starting the centrifuge ............................................ 35
Unbalance detection............................................... 35
Changing the settings during the run ..................... 35
Stopping the centrifuge .......................................... 36
Stopping with preset time ................................... 36
Stopping with continuous operation ................... 36
Short-time centrifugation ........................................ 36
Removing the rotor................................................. 37
Maintenance and care .............................. 39
Maintenance to be performed by the customer ..... 39
Cleaning.............................................................. 39
Disinfection ......................................................... 40
Decontamination................................................. 42
Autoclaving ......................................................... 43
The KENDRO service offer .................................... 44
Warranty conditions................................................ 44
Troubleshooting ....................................... 45
Emergency lid release............................................ 45
Problems you can handle yourself ......................... 47
In case you must call the Service........................... 54
Technical data........................................... 55
Electrical connections/fuses ................................... 56
Appendix ................................................... 57
Braking and acceleration curves ............................ 58
Speed / RCF diagrams........................................... 61
Autoclaving protocol ............................................... 67
Index .......................................................... 69

For your safety
3
For your safety
The SORVALL®centrifuges are manufactured accord-
ing to current technical standards and regulations.
Nonetheless, centrifuges may pose danger to individu-
als and surrounding if
• they are not used as designed
• they are operated by untrained personnel
• their design is improperly changed
• the safety instructions are not followed
Therefore, personnel involved with operation and
maintenance of the centrifuge must read and fol-
low the safety instructions.
In addition, the pertinent regulations for prevention of
accidents must be strictly followed.
This manual is an integral part of the centri-
fuge assembly and must be kept close at
hand at all times.
When damages at the power cord or at
casing were noticed the centrifuge has
to be set out of operation!
Proper use
The centrifuge is designed to separate liquid-
suspended materials having different densities and
particle size, respectively (maximum sample density is
1.2 g/cm³ {ml} at maximum speed).
Improper use
During a run, a safety zone of 30 cm around the centri-
fuge must be maintained where neither persons nor
hazardous materials may be present.
The centrifuge may cause harm to its user or other
persons or may damage goods if safety measures are
not followed:
Centrifuging hazardous materials
• The centrifuge is neither made inert, nor is it explo-
sion-proof. Therefore never use the centrifuge in an
explosion-prone environment.
• Do not centrifuge explosive or flammable sub-
stances. The same holds for substances prone to
react violently with each other.

For your safety
4
• Do not centrifuge toxic or radioactive substances or
pathogenic microorganisms without suitable safety
systems.
If microbiological samples of risk group II (accord-
ing to "Laboratory Bio-safety Manual" of WHO) are
being centrifuged, aerosol-tight bio-seals have to be
used.
For materials in a higher risk group, more than one
precaution is required.
• Should toxins or pathogenic substances enter the
centrifuge or its parts, you must perform appropri-
ate procedures for disinfection (see "Maintenance
and care – Disinfection").
• Strongly corrosive substances that may cause
damage to materials and reduce the mechanical
strength of the rotor may be centrifuged only inside
protective tubes.
Handling the centrifuge
• Use only original accessories for the centrifuge.
The only exceptions are common glass or plastic
centrifuge tubes if they are approved for the rotor
speed and RCF values.
• Never use the centrifuge unless the rotor is properly
installed.
• You may use the centrifuge only with a properly
loaded rotor. You must not overload the rotor.
• Strictly follow the rules and regulations for cleaning
and disinfection
• If the rotor or the rotor lid shows signs of corrosion
or wear, you must stop using it.
• Never open the lid manually if the rotor still turns.
• You may use the emergency lid release only in
case of emergency, e.g. during an interruption of
power supply
(see chapter "Troubleshooting").
• Never use the centrifuge with an opened lid.
• Never use the centrifuge if the front panel has been
partially or totally removed.
• Changes in mechanical or electrical components of
the centrifuge may only be carried out by individu-
als authorized by Kendro Laboratory Products.

For your safety
5
Conformity to current standards
SORVALLcentrifuges are manufactured and tested
according to the following standards and regulations:
- for all voltages
• IEC 61010
- for 120 V only
•
- for 230 V only
•
Details of the test standards take you please from the
technical data.
Safety instructions in this manual
This symbol denotes potential hazards to
persons.
This symbol denotes potential damage
to the centrifuge or parts in its immediate
surroundings.
General hints are marked with this
symbol.
In addition, you are asked to adhere to the pertinent
regulations, in Germany
• Regulations for prevention of accidents BGV A2
• Regulations for prevention of accidents VBG 5
• Regulations for prevention of accidents VBG 7z

For your safety
6
Notes

The Biofuge primo
7
The Biofuge primo
The figure below shows a general view of the Biofuge
primo with open lid and the rotor put into place.
Safety systems
The Biofuge primo is equipped with a number of safety
systems:
• Housing and rotor chamber manufactured from
impact-resistant plastic; inner armouring made of
steel
• Lid with window and lid lock
You can open the centrifuge lid only when the
power is turned on and the rotor has come to a halt.
You can start the centrifuge only if the lid is properly
locked.
• Rotor identification for power adjustment
• Electronic unbalance detection
• Warning if instrument is manually opened during a
run
If the lid is manually opened during a run, or if the
temperature of the drive exceeds a critical value, a
corresponding message appears in the display
("Lid" and "OPEN", respectively).
• Emergency lid release: only in case of emergency,
e.g. during power failure (see chapter "Trouble-
shooting"
Do not tamper with the safety
systems!

The Biofuge primo
8
for your notes

The Biofuge primo
9
Properties
The Biofuge primo is a laboratory centrifuge for use
with a variety of rotors and a large number of commer-
cially available centrifuge tubes.
The preset speed is reached in seconds. The mainte-
nance-free induction motor provides quiet and vibra-
tion-free operation even at high speeds and warrants
an extremely long lifetime.
The user-friendly "Easycontrol" control panel permits
easy preselection of speed, RCF value, run time and
run profile (acceleration and braking behavior). You
can switch from speed to RCF display or entry and
vice versa.
You can change the set values even during a run.
With the "quick run" key ( ) you can centrifuge a
sample for only a few seconds if that is required for
your particular task.
Items delivered
Items delivered with the centrifuge comprise:
− a special cap nut for fixing the rotor
− 10-mm tubular socket wrench for fastening
the cap nut
− power cord
The printed documents consist of the delivery notes
and this Manual.
cap nut
order no. 70056208
tubular socket wrench
order no. 2036 0072

The Biofuge primo
10
Functions and features
Part / function Description / feature
design / housing galvanized sheet chassis with armored shell and plastic housing placed on top
tank plastic
drive induction drive without carbon brushes
key and display board key and display elements covered by an easy-care protective foil
control microprocessor-driven by Easycontrol II
program memory the data last entered remain in memory
functions RCF preselection, quick run
acceleration profiles 2 acceleration and 8 braking curves
rotor identification automatic
unbalance detection electronic, optimally adjusted for each rotor as a function of speed
lid lock automatic locking upon pressing the lid shut

The Biofuge primo
11
The "Easycontrol" user interface
Function Feature
lid opening electromagnetic release via "open lid“ key when mains supply OK
(unlocking in case of power failure: see chapter "Troubleshooting")
start start key ( )
stop stop key ( )
short-term acceleration "quick run" key ( ): short-term run when permanently pressed; stop upon key
release
acceleration/braking profiles 1 = slow acceleration and slowest braking curve (2)
2 - 9 = fast acceleration and 8 different braking profiles
speed selection adjustable in steps of 10 min-1 within the range of 300 min-1 to 15000 min-1
RCF display upon actuating the switchover key, the current RCF value is displayed
run time selection adjustable in minutes from 1 min to 9 h 59 min; "hLd" mode: permanent operation
run time display in "quick run" mode between 1 s and 60 s in seconds steps, above in minutes
end of run speed display reads "End"

The Biofuge primo
12
Function Feature
diagnostic messages
• incorrectly closed lid: display "OPEN"
• general malfunction
(error messages ERROR codes, see chapter "Troubleshooting"

Before use
13
Before use
Transport and installation
The centrifuge is delivered in an special box. Cut it
open and remove the protective material.
Damage to the centrifuge by jolting!
Transport the centrifuge only in the
upright position using the special box
provided with the instrument and se-
cure it properly. Place the centrifuge
carefully.
Proper location
The location for the centrifuge must meet the following
criteria:
• A safety zone of at least 30 cm around the centri-
fuge must be maintained where neither persons nor
hazardous materials may be stationed during cen-
trifugation.
• The substructure must be stable and resonance-
free. A good support is provided by a plane labora-
tory bench or a large laboratory carriage with lock-
able casters.
• To ensure sufficient air circulation, a minimum dis-
tance from the wall of 10 cm at the back and of 15
cm on each side must be kept.
• The centrifuge must be protected from heat and
direct sunshine.
• The location must be well ventilated at all times.

Before use
14
Mains connection
Make sure that voltage and frequency correspond to
the specifications on the instrument label.
Turn the mains switch on the back panel off (press
"0"); only then connect the centrifuge with the mains
supply via the power cord.
mains switch
type plate with specifications on
mains voltage and frequency
socket for mains cable

Accessories
15
Accessories
The Biofuge primo is delivered without rotor!
You may choose from among a large variety of rotors
available as accessories (see Rotor program, Table 1).
In addition, there are sets of adapters and reduction
sleeves for diverse commercially available vessels
(see Adapters, Table 2).
Please consult our sales documentation for a complete
collection of accessories including technical data and
order numbers.
For more information you can visit our web site at
http://www.Kendro.com

Accessories
16
Rotor program
Table 1: Rotor program (1)
Rotor designation
Highconic®rotor
6 x 50 ml
swinging bucket rotor
4 x 100 ml
drum rotor
order no. 75007588 75007591 75007595
buckets and caps see Table 2
maximum permissible load [ g ] 6 x 130 4 x 200 8 x 80
maximum speed nmax [ min-1 ] 8500 4000 12 000
maximum RCF value at nmax 10 016 2522 14 005
maximum radius [ cm ] 12.4 14.1 8.7
minimum radius [ cm ] 6.0 5.0 3.8
angle [ ° ] 45 90 90 / 60
acceleration time [ s ] 55 25 40
braking time [ s ] 35 20 30
aerosol-tight 1) yes (reduced filling) yes no
permissible temperature range
autoclavable (number of cycles)
–
121 °C; (unlimited)
–
121 °C; (unlimited)
–
no
1) Checked by CAMR, Porton-Down, UK