Kendro Sorvall Legend T User manual

SORVAL
L
®
Legend™ T / RT Instruction Manual
QUIKset®

How to use this manual
Use this manual to get acquainted with your centri-
fuge and its accessories.
The manual helps you to avoid inappropriate han-
dling. Make sure to keep it always close to the centri-
fuge.
A manual that is not kept handy cannot provide
protection against improper handling and thus
against damage to persons and objects.
The manual comprises chapters on
• Safety regulations
• Instrument description
• Rotor program and accessories
• Transportation and hook-up
• Use of the centrifuge
• Maintenance and care
• Troubleshooting
• Technical data
• Index
Please fold out
Overleaf you will find a graphic
representation of the control panel
with a survey of the most important
functions

speed/RCF
Quick run
run time
open lid
stop
start
The mains switch is located on the right-hand
side panel.
Pressed up = ON I
Pressed down = OFF 0
(only for instruments with refrigeration unit)
temperature
Bucket selection rpm/RCF
switch key
display
Before switching on the centrifuge
please read this manual
deceleration
profiles
Set value input

Control panel of the Legend™ T / RT
Display panels
Deceleration profiles (1= slowest ...... 3= fastest)
permanent
display: deceleration profile last set 1 – 3
Speed / RCF
run: current value of speed or RCF after activation of
switch key
end: "End"
lid open: "OPEN"
"Lift Lid" (if lid is not automatically lifted off)
(before start)
lid closed: "0" with flashing point
(rotor not yet identified)
error code: will flash in display
Run time
time selection: - remaining run time to 0
continuous - run time passed (HLD)
operation (hld) (in hours, minutes)
"Quick Run": - run time passed (as long as button is held;
in minutes and seconds)
Temperature*
run: current sample temperature in °C
(in temperature equilibrium)
* only for instruments with refrigeration unit
Keys
start : normal start of the centrifuge
stop : manual stop of a run
open lid: open lid (possible only with the instrument
switched on)
Quick run: short-term operation of the centrifuge as long as
key remains pressed
rpm/RCF switch: switching between rpm and RCF display
Bucket set.: Setting of the bucket number
"set" keys: stepwise increase/decrease of setpoint values
deceleration profiles : switching the deceleration profiles
Adjusting knobs
Set value input: setting the respective set values.
Short pressing of one of the adjusting knobs ef-
fects switching from present to set value on the
set value display, signalised by flashing display.
Error codes (troubleshooting see chapter "Troubleshooting"):
E-19: Unpermissible rotor
E-20: Rotor not identified
E-29: Motor or rotor blocked
E-31: Overtemperature in motor
E-32: Overtemperature in electronic
E-33: Excessive pressure in the refrigeration unit
E-34: Overvoltage
E-35: Overcurrent
Lift Lid : Smoothly lift the lid
rotor: set speed higher than permissible speed of
the rotor
bAL : imbalance

Contents
1
Contents
For your safety............................................ 3
Proper use................................................................ 3
Improper use ............................................................ 3
Centrifuging hazardous materials ............................ 3
Handling the centrifuge ............................................ 4
Conformity to current standards............................... 5
Safety instructions in this manual ............................ 5
The Legend™ T / RT................................... 7
Description ............................................................... 7
Safety systems......................................................... 8
Parts supplied .......................................................... 8
Function and features .............................................. 9
Before use................................................. 13
Centrifuge transport and installation ...................... 13
Proper location ....................................................... 13
Main connection ..................................................... 14
Rotors and accessories ........................... 15
Rotors for the Legend™ T...................................... 16
Rotors for the Legend™ RT................................... 21
Handling the rotor................................................... 31
Aerosol-tight operation........................................... 36
Checking of aerosol-tight bio-containment......... 39
Operation .................................................. 41
Switching on the centrifuge .................................... 41
Actuating the lid...................................................... 41
Opening the lid ................................................... 41
Closing the lid ..................................................... 41
Installing the rotor................................................... 42
Loading the rotor .................................................... 43
Maximum loading ............................................... 43
Filling the centrifuge tubes ................................. 44
Maximum permissible load difference................ 44
Inserting the centrifuge tubes ............................. 45
Entering parameters............................................... 47
Deceleration curves ............................................... 47
Switching from speed to RCF display .................... 47
Bucket selection for swinging bucket rotors........... 47
Selecting speed...................................................... 48
Entering the RCF value.......................................... 48
More about the RCF value ................................. 49
Selecting run time .................................................. 49
Run time selection.............................................. 49
Continuous operation ......................................... 50
Extended time mode........................................... 50
Selecting the temperature ...................................... 51
Starting the centrifuge ............................................ 51
Imbalance display .................................................. 52

Contents
2
Changing the settings during the run ..................... 52
Stopping the centrifuge .......................................... 52
Stopping with preset run time............................. 52
Stopping with continuous operation ................... 52
Temperature control during standby ...................... 53
”Quick Run” ............................................................ 53
Removing the rotor................................................. 54
Audible alarm ......................................................... 54
Turning the centrifuge off ....................................... 55
Maintenance and care .............................. 57
Maintenance to be performed by the customer ..... 57
Cleaning.............................................................. 57
Disinfection ......................................................... 58
Decontamination................................................. 60
Autoclaving ......................................................... 60
The KENDRO service offer .................................... 61
Warranty conditions................................................ 61
Troubleshooting ....................................... 63
Emergency lid release............................................ 63
Error troubleshooting.............................................. 65
Contacting Kendro Service..................................... 74
Technical Data .......................................... 75
Electrical connections / fuses................................. 77
Appendix ................................................... 79
Deceleration profiles............................................... 79
Speed/RCF diagrams............................................. 85
Index .......................................................... 91

For your safety
3
For your safety
The SORVALL®centrifuges are manufactured accord-
ing to current technical standards and regulations.
Nonetheless, centrifuges may pose danger to individu-
als and surrounding if
• they are not used as designed
• they are operated by untrained personnel
• their design is improperly changed
• the safety instructions are not followed
Therefore, personnel involved with operation and
maintenance of the centrifuge must read and follow
the safety instructions.
In addition, the pertinent regulations for prevention of
accidents must be strictly followed
This manual is an integral part of the centri-
fuge assembly and must be kept close at
hand at all times.
When damages at the power cord or at
casing were noticed the centrifuge has
to be set out of operation!
Proper use
The centrifuge is designed to separate liquid-
suspended materials having different densities and
particle size, respectively (maximum sample density is
1.2 g/cm³ {ml} at maximum speed).
Improper use
During a run, a safety zone of 30 cm around the centri-
fuge must be maintained where neither persons nor
hazardous materials may be present.
The centrifuge may cause harm to user or other per-
sons or may damage goods if safety measures are not
followed:
Centrifuging hazardous materials
• The centrifuge is neither made inert, nor is it explo-
sion-proof. Therefore never use the centrifuge in an
explosion-prone environment.
• Do not centrifuge explosive or flammable sub-
stances must not be centrifuged. The same holds
for substances prone to react violently with each
other.

For your safety
4
• Do not centrifuge toxic or radioactive substances or
pathogenic microorganisms without suitable safety
systems.
If microbiological samples of risk group II (according
to "Laboratory Bio-safety Manual" of WHO) are be-
ing centrifuged, aerosol-tight bio-seals have to be
used.
For materials with a higher risk group, more than
one precaution is required.
• Should toxins or pathogenic substances enter the
centrifuge or its parts, you must perform appropriate
procedures for disinfection (see "Maintenance and
care – Disinfection").
• Strongly corrosive substances that may cause
damage to materials and reduce the mechanical
strength of the rotor, may be centrifuged only inside
protective tubes.
Handling the centrifuge
• Use only original accessories for the centrifuge. The
only exception are common glass or plastic centri-
fuge tubes, if they are approved for the rotor speed
and RCF values.
• Never use the centrifuge unless the rotor is properly
installed.
• You may use the centrifuge only with a properly
loaded rotor. You must not overload the rotor.
• Strictly follow the rules and regulations for cleaning
and disinfection
• If the rotor or the rotor lid shows signs of corrosion
or wear, you must stop using it.
• Never open the lid manually if the rotor still turns.
• You may use the emergency lid release only in
case of emergency, e.g. during an interruption of
power supply
(see chapter "Troubleshooting").
• Never use the centrifuge with the lid open.
• Never use the centrifuge if the front panel has been
partially or totally removed.
• Changes in mechanical or electrical components of
the centrifuge may be carried out only by individuals
authorized by Kendro Laboratory Products.

For your safety
5
Conformity to current standards
SORVALL®centrifuges are manufactured and tested
according to the following standards and regulations:
- for all voltages
• IEC 61010
- for 110 V only
•
- for 230 V only
•
Details of the test standards take you please from the
technical data.
Safety instructions in this manual
This symbol denotes potential hazards to
persons.
This symbol denotes potential damage to
the centrifuge or parts in its immediate
surroundings.
General hints are marked with this
symbol.
In addition, you are asked to adhere to the pertinent
regulations, in Germany
• Regulations for prevention of accidents BGV A2
• Regulations for prevention of accidents VBG 5
• Regulations for prevention of accidents VBG 7z
- with cooled devices additionally
• Regulations for prevention of accidents BGV D4

For your safety
6
Notes

Description of the Legend™
7
The Legend™ T / RT
The figure below shows a Legend™ RT with the lid
open and a swinging bucket rotor installed.
Description
The Legend™ RT is a general-purpose tabletop centri-
fuge for biotechnological and pharmaceutic research
that moves high capacity centrifugation onto the fast
track. It spins more tubes, at higher RCFs, more rapidly
than competitive instruments, and can process nomi-
nally three liters of sample in a single run. There are
various rotors available that can achieve high RCFs
and accommodate a wide range of accessories for all
common tube types, micro titer and deep well plates.
The user-friendly "QUIKset®" control panel permits
easy selection of speed, RCF value, run time and
deceleration profile, as well as temperature of the
Legend™ RT. You can switch from speed to RCF
display and vice versa, with a touch of a button and
even during a run.

Description of the
8
Safety systems
The Legend™ is equipped with a number of safety
systems:
• Housing and lid is constructed of 8 mm steel.
• Lid with window
• Lid lock with safety check
You can open the centrifuge lid only when the
power is turned on and the rotor has come to a
stop. You can start the centrifuge only if the lid is
properly locked.
• Automatic rotor identification
• Electronic imbalance detection as a function of rotor
(SMARTspinTM)
Do not tamper with the safety
system!
Parts supplied
Accessories supplied with the centrifuge are:
− power cord
− a special wrench for securing the rotor
(as seen in picture)
− condensed operating instructions
− corrosion protective oil
The printed documents consist of the delivery notes
and this Manual.

Description of the Legend™
9
Function and features
Basic unit/ function Description / feature
Cabinet / frame galvanized steel
Chamber stainless steel
Drive Brushless induction drive
Key pad and display key pad and display elements covered by an easy-care continuous surface
Control Microprocessor driven by "QUIKset®"
Main memory Recalls last run parameters
Advanced features RCF-programming, quick run, pretemp *, temperature control during standby
Deceleration profiles 3 deceleration profiles
Rotor identification automatic
SMARTspinTM
imbalance detection system
electronic, effective as a function of rotor and speed
Soft touch lid lock motor assisted lid locking
( * only with refrigeration unit )

Description of the
10
The QUIKset®user interface
Function Feature
Deceleration profile 1 = slowest, ... 3 = fastest deceleration curve
Setting speed by rpm adjustable from 300 rpm to 15 000 rpm, in 10 rpm increments
RCF selection upon activation of RCF switch , the RCF value can then be entered
Time selection adjustable in minutes from 1 min to 9 h 59 min,
or extended time mode from 1 min to 99 h,
”hld“-mode: continuous operation
Run time display in "quick run“ mode between 1 s and 60 s in seconds´ steps, above in minutes´ steps
Setting temperature * adjustable from -9°C to +40°C, in one degree increments
End of centrifugation the speed display will read "End“
( * only with refrigeration unit)

Description of the Legend™
11
Function Feature
Lid opening automatic unlocking via ”Open lid“ key ( )
(unlocking in case of power failure: see chapter ”Troubleshooting“)
Start start key ( )
Stop stop key ( )
"Quick Run“ mode pressing the "Quick Run“ key ( ) activates maximum acceleration up to the
maximum permissible speed of rotor; upon key release centrifuge stops with
maximum deceleration power.
Diagnostic messages
• alternating display ”Rotor“/maximum speed or RCF
(speed selected exceeds max. speed of the rotor)
• lid has not been lifted off the lock during opening:
display ”Lift lid“ (manual lifting of lid required)
• general instrument malfunction
(error messages with ERROR codes, see “Troubleshooting“)

Description of the
12
Notes

Before use
13
Before use
Centrifuge transport and installation
After opening the box remove the protective materials.
When transporting the centrifuge,
consider its weight (see „Technical
Data“); always grab it on both sides
taking care that enough helpers are
around.
Do not lift on the front panel!
The centrifuge can be damaged by
jolting during the transport!
Transport the centrifuge only in the
upright position using proper con-
tainment and secure it properly.
Handle the centrifuge carefully.
Proper location
The centrifuge may only be used indoors. Its location
must meet the following criteria:
• A safety zone of at least 30 cm (12 inches) around
the centrifuge must be maintained. Hazardous ma-
terials must not be stored beside unit while
centrifugation.
• The laboratory bench or centrifuge trolley must be
stable and resonance-free. A good support is pro-
vided by a laboratory bench or a centrifuge cart with
lockable casters.
• To ensure sufficient air circulation, a minimum dis-
tance from the wall of 10 cm (4 inches) at the back
and of 15 cm (6 inches) on each side must be kept.

Before use
14
• The centrifuge must be protected from heat and
direct sunlight.
• The location must be well ventilated at all times.
UV rays reduce the durability of
plastics.
Protect the centrifuge, rotors and
plastic accessories from direct
sunlight.
Main connection
Connect the centrifuge only to an grounded main
power supply. Make sure that the power cord is com-
patible with the safety regulations valid, and that your
main voltage and frequency correspond to the specifi-
cations on the instrument label.
Make sure that the centrifuge is switched off (on the
right-hand side of front panel) before connecting
electrical wire to main power supply.
RS232 interface
(optional)
specifications Main connection

Rotors and accessories
15
Rotors and accessories
A rotor is not included as part of a Legend™ T or RT
centrifuge.
A large variety of rotors are available as accessories.
In addition, there are adapters and reduction sleeves
for a variety of commercially available tubes and
bottles.
For a complete list of available tubes, bottles, adapt-
ers, and accessories refer to our current sales
documentation.
For more information you can visit our web site at
http://www.Kendro.com
Legend™ T (air cooled)
During centrifugation, heat is generated by air friction
between the rapidly spinning rotor and the air inside
the rotor chamber. The continuous air flow in the air
cooled centrifuge is restricting the temperature rise of
the samples. The equilibrium temperature rise de-
pends on the ambient temperature, the type of rotor,
the speed, the duration and the number of runs.
Use caution when touching rotors as
they may be hot after long runs at
high speed.

Rotors and accessories
16
Rotors for the Legend™ T
Table 1: Rotors for Legend™ T 100V / 120V (differences of 230V instruments are shown in parentheses)
Rotor designation Swinging Bucket Rotor 7500 6445
(7500 6434 with integrated locking mechanism)
With bucket
Order no.
Round bucket
750 ml
7500 6441
Rectangular bucket
250 ml
7500 6446
Carrier for
microplates
7500 6449
Maximum permissible load [ g ] 4 x 800 4 x 750 4 x 500
Maximum speed nmax [ rpm ] 4150 ( 4350 ) 3750 ( 4350 ) 4150 ( 4350 )
Maximum RCF value at nmax 3696 ( 4083 ) 2986 ( 4020 ) 3061 ( 3364 )
Radius (max.) [ cm ] 19.2 19.0 15.9
Acceleration / deceleration time [ s ] 60 / 30 ( 40 / 40 ) 60 / 30 ( 45 / 40 ) 50 / 25 ( 40 / 35 )
Heating of samples at nmax [ °C ]
relative to room temperature 23°C,
run time 1 hour
7 ( 9 ) 7 ( 10 ) 4 ( 5 )
Aerosol-tight * yes (with cap 75006421) yes (with cap 75006448) no
Autoclavable 121°C 121°C 121°C
* Checked by TÜV (Technical Supervision Association) Product Service GmbH – Hamburg