Bruker SamplePro hr-MAS User manual

Version
Innovation with Integrity
NMR Spectroscopy
SamplePro hr-MAS
001
Sample Changer
User Manual

Copyright © by Bruker Corporation
All rights reserved. No part of this publication may be
reproduced, stored in a retrieval system, or transmitted, in any
form, or by any means without the prior consent of the publisher.
Product names used are trademarks or registered trademarks of
their respective holders.
This manual was written by
Gilbert Cyppel and Stanley J. Niles
© November 8, 2010: Bruker Biospin GmbH
Rheinstetten, Germany
P/N: Z31914
DWG-Nr.: Z4D11177
For further technical assistance on the SamplePro hr-MAS unit,
please do not hesitate to contact your nearest BRUKER dealer
or contact us directly at:
BRUKER BioSpin GMBH
am Silberstreifen
D-76287 Rheinstetten
Germany
Phone: + 49 721 5161 0
FAX: + 49 721 5171 01
E-mail: [e-mail]
Internet: www.bruker.com

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Table of Contents
Contents
1 About ..............................................................................................................7
1.1 This Manual.............................................................................................................. 7
1.2 Policy Statement ...................................................................................................... 7
1.3 Symbols and Conventions........................................................................................ 7
2 Introduction....................................................................................................9
2.1 Concept.................................................................................................................... 9
2.2 Limitations of Liability............................................................................................. 10
2.3 Copyright................................................................................................................ 10
2.4 Warranty Terms...................................................................................................... 10
2.5 Customer Service................................................................................................... 10
3 Safety............................................................................................................11
3.1 Intended Use..........................................................................................................11
3.2 Owner‘s Responsibility........................................................................................... 11
3.3 Personnel Requirements........................................................................................ 13
3.3.1 Qualifications.......................................................................................................... 13
3.3.2 Unauthorized Persons............................................................................................ 14
3.3.3 Instruction...............................................................................................................14
3.4 Personal Protective Equipment.............................................................................. 14
3.5 Basic Dangers........................................................................................................ 15
3.5.1 Location of the Robot ON/OFF Switch................................................................... 15
3.5.2 General Workplace Dangers.................................................................................. 16
3.6 Environmental Protection ....................................................................................... 19
3.7 Signage .................................................................................................................. 20
3.7.1 Warning Signs........................................................................................................ 20
4 Technical Data .............................................................................................21
4.1 General................................................................................................................... 21
4.2 Electrical................................................................................................................. 22
4.3 Operating Conditions.............................................................................................. 23
4.4 Rating Plate System............................................................................................... 23
4.5 Rating Plate Robot................................................................................................. 24
4.6 Rating Plate Exchange Unit ................................................................................... 24
5 Design and Function...................................................................................25
5.1 Overview ................................................................................................................ 25
5.2 Brief Description..................................................................................................... 26
5.3 System Description ................................................................................................ 26
5.4 Description of The SamplePro Robotic System ..................................................... 27
5.4.1 The Robot Back Panel ........................................................................................... 28
5.4.2 The SamplePro LED Status Light .......................................................................... 28

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5.4.3 The Door Lock........................................................................................................29
5.5 The SamplePro Exchange Unit..............................................................................29
5.5.1 Exchange Unit Back Panel.....................................................................................29
5.6 Description of the MAS Pneumatic Control Unit.....................................................31
5.6.1 COM Mode.............................................................................................................31
5.7 Description of the hr-MAS Probe............................................................................32
6 Transport, Packaging and Storage............................................................33
6.1 Symbols on the Packaging.....................................................................................33
6.2 Inspection at Delivery.............................................................................................35
6.3 Packaging...............................................................................................................36
6.4 Storage...................................................................................................................36
7 Installation and Initial Commissioning......................................................37
8 Operation Overview.....................................................................................39
8.1 Safety .....................................................................................................................39
8.1.1 Emergency Shutdown ............................................................................................39
8.2 General Operating Guidelines................................................................................40
8.2.1 Switching On the Device ........................................................................................40
8.2.2 Switching Off the Device ........................................................................................40
9 Assembling the Tray with Rotors ..............................................................41
10 Operation......................................................................................................45
10.1 SamplePro hr-MAS Main Display...........................................................................45
10.1.1 System View...........................................................................................................46
10.1.2 Holder View............................................................................................................47
10.1.3 Sample Holder List.................................................................................................48
10.1.4 Right Window Panel...............................................................................................48
10.1.4.1 Container Information.............................................................................................48
10.1.4.2 Robot Information...................................................................................................50
10.1.4.3 Exchange Unit Information.....................................................................................50
10.1.4.4 Nitrogen/Air Flow Information.................................................................................51
10.1.4.5 Cooling Temperature Information...........................................................................51
10.1.5 Menu and Button Navigation Overview..................................................................52
10.2 Basic Commands ...................................................................................................53
10.2.1 Insert Sample .........................................................................................................54
10.2.2 Eject Sample ..........................................................................................................55
10.3 Exchanging Rotors and rotor containers................................................................55
10.4 Activity History........................................................................................................56
11 Preventative Maintenance ..........................................................................59

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11.1 Safety..................................................................................................................... 59
11.1.1 Cleaning and Decontamination.............................................................................. 60
11.1.2 Weekly Maintenance.............................................................................................. 60
11.1.3 Cleaning and Lubrication........................................................................................ 60
11.1.3.1 Pickup Adapter and Object Sensor Z-racks ........................................................... 60
11.2 Cleaning the Outside of the SamplePro hr-MAS and Units.................................... 61
11.2.1 After Cleaning......................................................................................................... 61
11.2.2 Other Cleaning Operations..................................................................................... 61
11.3 Cleaning the Robot Barcode Reader ..................................................................... 61
12 Dismantling and Disposal...........................................................................63
12.1 Repair Declaration Form........................................................................................ 63
13 EC-Declaration of Conformity ....................................................................65
14 Contact .........................................................................................................67
Appendix A...........................................................................................................69
A.1 Warning Signs........................................................................................................ 69
A.2 Figures ................................................................................................................... 71
A.3 Tables..................................................................................................................... 73
A.4 Glossary ................................................................................................................. 75
A.5 Index.......................................................................................................................77

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Table of Contents

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1 About
1.1 This Manual
This manual enables safe and efficient handling of the device.
This manual is an integral part of the device, and must be kept in close proximity to the
device where it is permanently accessible to personnel.
Before starting any work, personnel must have read the manual thoroughly and under-
stood its contents. Compliance with all specified safety instructions and operating
instructions is vital to ensure safe operation.
In addition, local accident prevention regulations and general safety instructions must be
observed for the operational area of the device.
Illustrations in this manual are intended to facilitate basic understanding, and may differ
from the actual design.
This user manual must be kept with the device. In addition to the user manual, instruc-
tions concerning labor protection laws, operator regulations tools and supplies must be
available and adhered to.
1.2 Policy Statement
It is the policy of Bruker to improve products as new techniques and components
become available. Bruker reserves the right to change specifications at any time.
Every effort has been made to avoid errors in text and figure presentation in this publica-
tion. In order to produce useful and appropriate documentation, we welcome your com-
ments on this publication. Support engineers are advised to regularly check with Bruker
for updated information.
Bruker is committed to providing customers with inventive, high quality products and ser-
vices that are environmentally sound.
1.3 Symbols and Conventions
Safety instructions in this manual are marked with symbols. The safety instructions are
introduced using indicative words which express the extent of the hazard.
In order to avoid accidents, personal injury or damage to property, always observe safety
instructions and proceed with care.
DANGER
This combination of symbol and signal word indicates an immediately hazardous sit-
uation which could result in death or serious injury unless avoided.

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About
iThis symbol highlights useful tips and recommendations as well as information designed
to ensure efficient and smooth operation.
Special Safety Instructions
The following symbols are used in the safety instructions to draw attention to specific
danger:
WARNING
This combination of symbol and signal word indicates a potentially hazardous situa-
tion which could result in death or serious injury unless avoided.
CAUTION
This combination of symbol and signal word indicates a possibly hazardous situation
which could result in minor or slight injury unless avoided.
NOTICE
This combination of symbol and signal word indicates a possibly hazardous situation
which could result in damage to property or the environment unless avoided.
DANGER
This combination of symbol and signal word indicates dangers posed by electric
power. If the safety instructions are not observed, there is a danger of serious or fatal
injuries.

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2 Introduction
The SamplePro hr-MAS is a sample changer that was developed for high throughput,
high resolution, magic angle spinning (hr-MAS) applications, such as the measurement
of biological samples.
The new sample changer is part of a Bruker NMR system for standard NMR MAS appli-
cations and is used to transfer prepared MAS samples to and from Bruker NMR instru-
ments to ensure they stay in continuous operation.
2.1 Concept
The sample changer consists of a laboratory robot (SamplePro) and a transfer system
connecting the laboratory robot to the probe inside the NMR magnet.
The sample changer provides a Bruker standard interface, through which the changer
can be controlled from TopSpin and IconNMR software.
Refer to "Design and Function" on page 25 for a complete description of the design and
function of SamplePro hr-MAS.
Figure 2.1 SamplePro hr-MAS

10 Z31914_00_01
Introduction
2.2 Limitations of Liability
All specifications and instructions in this manual have been compiled taking account of
applicable standards and regulations, the current state of technology and the experience
and insights we have gained over the years.
The manufacturer accepts no liability for damage due to:
• Failure to observe this manual.
• Improper use.
• Deployment of untrained personnel.
• Unauthorized modifications.
• Technical modifications.
• Use of unauthorized spare parts.
The actual scope of supply may differ from the explanations and depictions in this man-
ual in the case of special designs, take-up of additional ordering options, or as a result of
the latest technical modifications.
The undertakings agreed in the supply contract, as well as the manufacturer's Terms
and Conditions and Terms of Delivery, and the legal regulations applicable at the time of
the conclusion of the contract shall apply.
2.3 Copyright
This manual is protected by copyright and intended solely for internal use.
This manual must not be made available to third parties, duplicated in any manner or
form – whether in whole or in part – and the content must not be used and/or communi-
cated, except for internal purposes, without the written consent of the manufacturer.
Violation of the copyright will result in legal action for damages. We reserve the right to
assert further claims.
2.4 Warranty Terms
The warranty terms are included in the manufacturer's Terms and Conditions.
2.5 Customer Service
Our customer service division is available to provide technical information. See "Con-
tact" on page 67 for contact details.
In addition, our employees are always interested in acquiring new information and expe-
rience gained from practical application; such information and experience may help
improve our products.

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3 Safety
This section provides an overview of all the main safety aspects involved in ensuring
optimal personnel protection and safe and smooth operation.
Non-compliance with the action guidelines and safety instructions contained in this man-
ual may result in serious hazards.
3.1 Intended Use
The SamplePro hr-MAS sample changer has been designed and constructed solely for
the intended use described here.
The sample changer must only be used for keeping NMR samples in a specially
designed rotor container, inserting them into the NMR spectroscopy magnet and ejecting
them after measurement back into the rotor container.
Intended use also includes compliance with all specifications in this manual.
Any use which exceeds or differs from the intended use shall be considered improper
use.
No claims of any kind for damage will be entertained if such claims result from improper
use.
3.2 Owner‘s Responsibility
Owner
The term „owner“ refers to the person who themselves operates the unit for trade or
commercial purposes, or who surrenders the unit to a third party for use/application, and
who bears the legal product liability for protecting the user, the personnel or third parties
during the operation.

12 Z31914_00_01
Safety
Owner‘s Obligations
The device is used in the industrial sector, universities and research laboratories. The
owner of the device must therefore comply with statutory occupational safety require-
ments.
In addition to the safety instructions in this manual, the safety, accident prevention and
environmental protection regulations governing the operating area of the device must be
observed.
In this regard, the following requirements should be particularly observed:
• The owner must obtain information about the applicable occupational safety regula-
tions, and - in the context of a risk assessment - must determine any additional dan-
gers resulting from the specific working conditions at the usage location of the
device. The owner must then implement this information in a set of operating
instructions governing operation of the device.
• During the complete operating time of the device, the owner must assess whether
the operating instructions issued comply with the current status of regulations, and
must update the operating instructions if necessary.
• The owner must clearly lay down and specify responsibilities with respect to installa-
tion, operation, troubleshooting, maintenance and cleaning.
• The owner must ensure that all personnel dealing with the device have read and
understood this manual. In addition, the owner must provide personnel with training
and hazards information at regular intervals.
• The owner must provide the personnel with the necessary protective equipment.
• The owner must warrant that the device is operated by trained and authorised per-
sonnel as well as all other work, such as transportation, mounting, start-up, the
installation, maintenance, cleaning, service, repair and shutdown, that is carried out
on the device.
• All personnel who work with, or in the close proximity of the device, need to be
informed of all safety issues and emergency procedures as outlined in this user
manual.
• The owner must document the information about all safety issues and emergency
procedures in a laboratory SOP (Standard Operating Procedure). Routine briefings
and briefings for new personnel must take place.
• The owner must ensure that new personnel are supervised by experienced person-
nel. It is highly recommended to implement a company training program for new
personnel on all aspects of product safety and operation.
• The owner must ensure that personnel are regularly informed of the potential haz-
ards within the laboratory. This is all personnel that work in the area, but in particular
laboratory personnel and external personnel such as cleaning and service person-
nel.
• The owner is responsible for taking measures to avoid inherent risks in the handling
of dangerous substances, preventing industrial disease, and providing medical first
aid in emergencies.
• The owner is responsible for providing facilities according to the local regulations for
the prevention of industrial accidents and generally accepted safety regulations
according to the rules of occupational medicine.

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Safety
• All substances needed for operating and cleaning the device samples, solvents,
cleaning agents, gases, etc. have to be handled with care and disposed of appropri-
ately. All hints and warnings on storage containers must be read and adhered to.
• The owner must ensure that the work area is sufficiently illuminated to avoid reading
errors and faulty operation.
• The owner must ensure that the laboratory is equipped with an oxygen warning
device, in case the device is operated with nitrogen.
Furthermore, the owner is responsible for ensuring that the device is always in a techni-
cally faultless condition. Therefore, the following applies:
• The owner must ensure that the maintenance intervals described in this manual are
observed.
• The owner must ensure that all safety devices are regularly checked to ensure full
functionality and completeness.
3.3 Personnel Requirements
3.3.1 Qualifications
iNote: Only trained Bruker personnel are allowed to mount, retrofit, repair, adjust and dis-
mantle the unit!
This manual specifies the personnel qualifications required for the different areas of
work, listed below:
Laboratory Personnel
Laboratory personnel are health care professionals, technicians, and assistants staffing
a research or health care facility where specimens are grown, tested, or evaluated and
the results of such measures are recorded. Laboratory personnel are able to carry out
assigned work and to recognize and prevent possible dangers self-reliant due to their
professional training, knowledge and experience as well as profound knowledge of
applicable regulations.
The workforce must only consist of persons who can be expected to carry out their work
reliably. Persons with impaired reactions due to, for example, the consumption of drugs,
alcohol, or medication are prohibited from carrying out work on the device.
When selecting personnel, the age-related and occupation-related regulations governing
the usage location must be observed.

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Safety
3.3.2 Unauthorized Persons
3.3.3 Instruction
Personnel must receive regular instruction from the owner. The instruction must be doc-
umented to facilitate improved verification.
3.4 Personal Protective Equipment
Personal protective equipment is used to protect the personnel from dangers which
could affect their safety or health while working.
Personnel must wear personal protective equipment while carrying out the different
operations at and with the device.
This equipment will be defined by the head of the laboratory. Always comply with the
instructions governing personal protective equipment posted in the work area.
WARNING
Risk to life for unauthorized persons due to hazards in the danger and
working areas.
Unauthorized persons who do not meet the requirements described here will not be
familiar with the dangers in the working zone. Therefore, unauthorized persons face
the risk of serious injury or death.
fUnauthorized persons must be kept away from the danger and working zone.
fIf in doubt, address the persons in question and ask them to leave the danger and
working zone.
fCease work while unauthorized persons are in the danger and working zone.
Date Name Type of
Instruction Instruction Provided By Signature

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Safety
3.5 Basic Dangers
The following section specifies residual risks which may result from using the device and
have been established by means of a risk assessment.
In order to minimize health hazards and avoid dangerous situations, follow the safety
instructions specified here as well as in the following chapters of this manual.
3.5.1 Location of the Robot ON/OFF Switch
The robot ON/OFF switch is located on the right side of the deck tray:
Figure 3.1 The Robot ON/OFF Switch

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Safety
3.5.2 General Workplace Dangers
NOTICE
Material damage hazard from overflow of cryogens.
Material damage may result from the overflow of cryogens.
fTurn off the unit during magnet servicing.
fCover the unit with a protective cover to avoid contact with cold gases.
fBe sure to use sufficient transfer line and Teflon evacuation hose for nitrogen and
helium refills based on recommendations in the magnet manual.
fAfter refilling cryogens some parts of the magnet may be icy. Be sure to remove
the ice to avoid its melting onto the unit.
CAUTION
Accident hazard from contact with hot or cold surfaces on the unit.
Contact with the hot or cold surfaces of the unit may result in serious burns.
fDo not touch unit parts of cooled or heated units.
fDo not use damaged units.
fAfter removing any part of the unit, allow it to cool or thaw before coming in con-
tact.
WARNING
Accident hazard from asphyxiation.
A break in a tube or hose may result in the uncontrolled exit of nitrogen into the labo-
ratory.
fAn oxygen warning device must be present in the laboratory if the device is oper-
ated with nitrogen.
fProper air ventilation must be planned and implemented in the laboratory.

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Safety
CAUTION
Accident hazard from breaking or spilled samples.
Samples may break or be spilled due to improper adjustment or cap loss. This may
result in personal injury or equipment contamination.
fFollow the mounting, installation and adjustment instructions in the manual.
fConfirm the correct installation at the customer site through use of acceptance
protocol.
fStandard operating procedures for sample preparation and usage must be imple-
mented.
fDuring installation and maintenance use only rotors with dummy samples.
Remove all MAS rotors (also from the magnet) before the installation and mainte-
nance!
WARNING
Biological, chemical hazard.
Infection, contamination, or other health endangerment as a result of contact with
biological or chemical substances, e.g. from broken samples.
fClean the unit before maintenance work and/or returning to Bruker for repair.
fPrepare a list of materials in which the unit came into contact with or measured.
fA signed confirmation of correctly carrying out cleaning/disinfection is required
from the customer. Without this confirmation the parts delivered for repair will be
rejected and returned to the customer.
NOTICE
Material damage hazard due to improper restart.
Device deadlock may occur as a result of restarting the unit when an equipment fail-
ure has occurred.
fWhen an error has occurred, do not try to restart the unit until the cause of the
error has been corrected.
fOnly trained personnel should carry out maintenance work.

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Safety
iNote: Reference measurements should be made at regular intervals to help detect pos-
sible vibration and/or electromagnetic interference.
DANGER
Accident and/or material damage hazard from sample breakage.
Contact with hazardous substances contained in sample rotors that break may result
in a life threatening situation or material damage. The contents of the sample rotors
are the responsibility of the laboratory supervisor. The laboratory supervisor is
responsible for:
fDefining disinfectant and cleaning procedures.
fEstablishing and enforcing the use of protective clothing (gloves, eye protection,
mask etc.).
fPreparing laboratory instructions for handling this type of accident.
fPreparing an emergency plan.
fEnforcing standard sample handling procedures, e.g. removing all samples from
the rotor container and using sample rotors with dummy contents before mainte-
nance work etc.
fTraining laboratory personnel.
NOTICE
Material damage hazard from icing due to lack of nitrogen.
Material damage may result from icing caused by a lack of nitrogen flow.
fThe nitrogen fill level must be controlled regularly.
fWhen icing occurs, the rotors must be defrosted according to established labora-
tory procedures.
NOTICE
Material damage hazard software error.
Samples or the unit may be damaged due to an software error causing malfunction of
the control system. Users may also be shocked by abrupt malfunction or unexpected
system start.
fSample rotors with dummy contents must be used during installation and service.
fPersonal should be alerted to unexpected malfunctions.

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Safety
iTo avoid mix-up of the contents in rotors, proper standard laboratory operating proce-
dures must be followed.
3.6 Environmental Protection
Helium Inert Gas
Helium inert gas may cause suffocation at high concentrations. Disposal of the empty
gas cylinders must be performed by a specialist disposal company.
Coolants
When released, coolants develop decomposition products which are hazardous to the
environment. Maximum care and caution are required when handling coolants. Always
observe the safety data sheet issued by the manufacturer. Ensure that personnel han-
dling coolants are regularly informed about potential dangers and are instructed in the
safe handling of coolants.
Cleaning Liquids
Cleaning liquids incorporating solvents contain toxic substances. They must not be
allowed to escape into the environment. Disposal must be carried out by a specialist dis-
posal company.
CAUTION
Danger to the environment from incorrect handling of pollutants!
Incorrect handling of pollutants, particularly incorrect waste disposal, may cause seri-
ous damage to the environment.
fAlways observe the instructions below regarding handling and disposal of pollut-
ants.
fTake the appropriate actions immediately if pollutants escape accidentally into the
environment. If in doubt, inform the responsible municipal authorities about the
damage and ask about the appropriate actions to be taken.

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Safety
3.7 Signage
3.7.1 Warning Signs
Warning indicating a danger zone in work rooms.
Warning indicating a biological hazard.
Warning indicating severe hand injury.