Labconco Purifier 3951400 User manual

Labconco Corporation
8811 Prospect Avenue
Kansas City, MO 64132-2696
800-821-5525, 816-333-8811
FAX [phone]
E-MAIL [e-mail]
HOME PAGE www.labconco.com
To receive important product updates,
complete your product registration card
online at register.labconco.com
User’s Manual
Purifier®Non-Ventilated
PCR & Tissue Culture Enclosures
Models
3951400
3951420
3952400
3952420
Please read the User’s Manual before operating the equipment.

Copyright © 2004, 2007, 2013, 2014 Labconco Corporation. All rights reserved.
The information contained in this manual and the accompanying products are copyrighted and all rights
reserved by Labconco Corporation. Labconco Corporation reserves the right to make periodic design
changes without obligation to notify any person or entity of such change.
Returned or Damaged Goods
Do not return goods without the prior authorization from Labconco. Unauthorized returns will not be
accepted. If your shipment was damaged in transit, you must file a claim directly with the freight carrier.
Labconco Corporation and its dealers are not responsible for shipping damages.
The United States Interstate Commerce Commission rules require that claims be filed with the delivery
carrier within fifteen (15) days of delivery.
Limitation of Liability
The disposal and/or emission of substances used in connection with this equipment may be governed by
various federal, state, or local regulations. All users of this equipment are required to become familiar with
any regulations that apply in the user’s area concerning the dumping of waste materials in or upon water,
land, or air and to comply with such regulations. Labconco Corporation is held harmless with respect to
user’s compliance with such regulations.
Contacting Labconco Corporation
If you have questions that are not addressed in this manual, or if you need technical assistance, contact
Labconco’s Customer Service Department or Labconco’s Product Service Department at 1-800-821-5525
or 1-816-333-8811, between the hours of 7:00 a.m. and 6:00 p.m., Central Standard Time.
Part #3939201, Rev. A
ECO J105
Warranty
Labconco provides a warranty on all parts and factory workmanship. The warranty includes areas of
defective material and workmanship, provided such defect results from normal and proper use of the
equipment. Glassware is not warranted from breakage when dropped or mishandled.
The warranty for Purifier®Non-Ventilated PCR & Tissue Culture Enclosures will expire one year
from date of installation or two years from date of shipment from Labconco, whichever is sooner.
This limited warranty covers parts and labor, but not transportation and insurance charges. In the
event of a warranty claim, contact Labconco Corporation or the dealer who sold you the product. If
the cause is determined to be a manufacturing fault, the dealer or Labconco Corporation will repair or
replace all defective parts to restore the unit to operation. Under no circumstances shall Labconco
Corporation be liable for indirect, consequential, or special damages of any kind. This statement may
be altered by a specific published amendment. No individual has authorization to alter the provisions
of this warranty policy or its amendments. Lamps and filters are not covered by this warranty.
Damage due to corrosion or accidental breakage is not covered.

ORIGINAL INSTRUCTIONS
TABLE OF CONTENTS
CHAPTER 1: INTRODUCTION 1
Polymerase Chain Reaction (PCR) Definition 1
About This Manual 2
Typographical Conventions 3
CHAPTER 2: PREREQUISITES 4
Support Requirements 4
Location and Air Current Requirements 4
Electrical Requirements 5
Space Requirements 5
CHAPTER 3: GETTING STARTED 6
Unpacking Your Enclosure 7
Installing the Enclosure on a Supporting Structure & Work Surface 7
Connecting the Electrical Supply 9
Sealing the Enclosure to the Work Surface 9
CHAPTER 4: FEATURES AND SAFETY PRECAUTIONS 10
Features 10
Safety Precautions 12
CHAPTER 5: USING YOUR ENCLOSURE 13
CHAPTER 6: MAINTAINING YOUR ENCLOSURE 14
Routine Maintenance Schedule 14
UV Light Replacement 14
Fluorescent Light Replacement 15
CHAPTER 7: ACCESSORIZING YOUR ENCLOSURE 16
Work Surfaces 16
Storage Cabinets 17
Base Stands & Mobile Stands 17

CHAPTER 8: TROUBLESHOOTING 18
APPENDIX A: REPLACEMENT PARTS 19
APPENDIX B: DIMENSIONS 22
APPENDIX C: SPECIFICATIONS 23
DECLARATION OF CONFORMITY 24

Product Service 1-800-522-7658
1
CHAPTER 1
INTRODUCTION
Congratulations on your purchase of a Labconco Purifier®Passive PCR Enclosure
or Purifier Tissue Culture Enclosure. Your non-ventilated enclosure is designed
to reduce cross-contamination in laboratory applications.
The Purifier Passive PCR Enclosure and Purifier Tissue Culture Enclosure offer a
UV light for disinfecting the interior and an interior switched electrical outlet. The
Purifier Passive PCR Enclosure UV light has the added feature of a digital timer to
accommodate PCR protocol. To take full advantage of all the features, please
acquaint yourself with this manual and keep it handy for future reference.
Polymerase Chain Reaction (PCR)
Definition
Polymerase Chain Reaction (PCR), is a laboratory process in which a particular
DNA segment from a mixture of DNA chains is rapidly replicated, producing a
large, readily analyzed sample of a piece of DNA. In PCR, DNA is immersed in a
solution containing the enzyme DNA polymerase, unattached nucleotide bases (the
subunits that DNA is composed of), and “primers”, short sequences of nucleotides
designed to bind with an end of the desired DNA segment. Two primers are used:
one primer binds at one end of the desired segment on one of the two paired DNA
strands and the other primer binds at the other end but on the other strand. The
solution is heated to break the bonds between the strands of the DNA. When the
solution cools, the primers bind to the separated strands, and DNA polymerase
quickly builds a new strand by joining the free nucleotide bases to the primers.
When this process is repeated, a strand that was formed with one primer binds to
the other primer, resulting in a new strand that is restricted solely to the desired
segment. Thus, the region of DNA between the primers is selectively replicated.
Further repetitions of the process can produce billions of copies of a small piece of
DNA in several hours. PCR was developed in 1985 by Kary B. Mullis, who was
awarded the 1993 Nobel Prize in chemistry for his work. It is used in a broad

Chapter 1: Introduction
Product Service 1-800-522-7658
2
2
range of applications from DNA fingerprinting to medical tests to identify diseases
from the infectious agent’s DNA. See also nucleic acid. The Concise Columbia
Encyclopedia is licensed from Columbia University Press. Copyright © 1995 by
Columbia University Press. All rights reserved.
About This Manual
This manual is designed to help you learn how to install, use, and maintain your
non-ventilated enclosure. Instructions for installing optional equipment on your
enclosure are also included.
If the unit is not operated as specified in this manual it may
impair the protection provided by the unit.
Si l'unité n'est pas utilisée comme spécifié dans ce manuel il
peut diminuer la protection fournie par l'unité.
Chapter 1: Introduction provides a brief overview of the non-ventilated enclosure,
explains the organization of the manual, and defines the typographical conventions
used in the manual.
Chapter 2: Prerequisites explains what you need to do to prepare your site before
you install the enclosure. Electrical and service requirements are discussed.
Chapter 3: Getting Started contains the information you need to properly unpack,
inspect and install the enclosure.
Chapter 4: Features and Safety Precautions explains how the enclosure operates
and the appropriate precautions you should take when using it.
Chapter 5: Using Your Enclosure discusses the basic operation of how to prepare,
use and turn off your non-ventilated enclosure is included.
Chapter 6: Maintaining Your Enclosure explains how to perform routine
maintenance.
Chapter 7: Accessorizing Your Enclosure explains acceptable modifications or
how to add accessories.
Chapter 8: Troubleshooting contains a table of problems you may encounter while
using the product including the probable causes of the problems and suggested
corrective actions.
Appendix A: Components contains labeled diagrams of all of the components of
the enclosures.
!

Chapter 1: Introduction
Product Service 1-800-522-7658
3
Appendix B: Dimensions show all of the dimensions for the enclosures.
Appendix C: Specifications contains the electrical requirements. Wiring diagrams
are also included in this section.
Typographical Conventions
Recognizing the following typographical conventions will help you understand and
use this manual:
Book, chapter, and section titles are shown in italic type (e.g., Chapter 3:
Getting Started).
Steps required to perform a task are presented in a numbered format.
Comments located in the margins provide suggestions, reminders, and
references.
Critical information is presented in boldface type in paragraphs that are
preceded by the exclamation icon. Failure to comply with the information
following an exclamation icon may result in injury to the user or permanent
damage to the enclosure.
Critical information is presented in boldface type in paragraphs that are
preceded by the wrench icon. A trained certifier or contractor should only
perform these operations. Failure to comply with the information following a
wrench icon may result in injury to the user or permanent damage to your
hood.
Important information is presented in capitalized type in paragraphs that are
preceded by the pointer icon. It is imperative that the information contained in
these paragraphs be thoroughly read and understood by the user.
The PCR icon indicates the text is specific to the Purifier PCR Enclosure.
The TC icon indicates the text is specific to the Purifier Tissue Culture
Enclosure.
CAUTION –See Manual. When this symbol is on the unit it indicates a
caution that is detailed in this manual.
ATTENTION - Voir manuel. Lorsque ce symbole est sur l'unité, il indique une
mise en garde qui est indiqué dans ce manuel.

!
PCR
TC
!

Product Service 1-800-522-7658
4
4
CHAPTER 2
PREREQUISITES
Before you install the enclosure, carefully examine the location. You must be
certain that the area is level and of solid construction. In addition, a dedicated
source of electrical power should be located near the installation site to power the
enclosure.
Carefully read this chapter to learn the requirements for your installation site:
The support requirements.
The location and air current requirements.
The electrical power requirements.
The space requirements.
Support Requirements
At a minimum, the supporting structure usually consists of a base cabinet and
chemically resistant work surface. A mobile stand or cart is allowable. See
Chapter 7 for accessory work surfaces, stands, mobile stands and cabinets.
Location and Air Current Requirements
The Purifier Passive PCR and Purifier Tissue Culture Enclosures have been
designed to reduce cross drafts and air circulation with the sash and UV sash
closure panel installed. However, as a precautionary measure of safety and a
higher level of quality management, it is recommended that the enclosure be
placed in such an area away from:
High traffic areas where walking might cause an air disturbance.
Overhead or wall HVAC diffusers, fans, radiators or other lab equipment
producing air currents.
Next to doorways or windows that may be opened.

Chapter 2: Prerequisites
Product Service 1-800-522-7658
5
Do not position the unit so that it is difficult to operate the main
disconnect device.
Ne placez pas l'appareil de sorte qu'il est difficile de faire
fonctionner le dispositif principal de déconnexion.
Electrical Requirements
Standard duplex electrical receptacles should be nearby for connecting the non-
ventilated Purifier Passive PCR Enclosure or Purifier Tissue Culture Enclosure.
For your convenience the enclosures have a switched auxiliary outlet receptacle.
The auxiliary switched outlet is rated for either 115V, 8 amps or 230V, 4 amps.
Space Requirements
The dimensions for the non-ventilated enclosures are shown in Appendix B:
Enclosure Dimensions.
!

Product Service 1-800-522-7658
6
6
CHAPTER 3
GETTING STARTED
Now that the site for your non-ventilated Purifier PCR or Purifier Tissue Culture
Enclosures is properly prepared, you are ready to unpack, inspect and install your
non-ventilated enclosure. Read this chapter to learn how to:
Unpack and move the enclosure.
Set up the enclosure with the proper supporting structure and work surface.
Connect the electrical supply.
Seal the enclosure to the work surface.
Depending upon which model you are installing, you may need wrenches, ratchets,
sockets, a nut driver set, a flat-blade screwdriver, a Phillips screwdriver, and a
carpenter level to complete the instructions in the chapter.
The enclosures weigh 100 lbs. (45 kg). The shipping container
allows for lifting with a mechanical lift truck or floor jack. If
you must lift the enclosure manually, follow safe-lifting
guidelines. Do not lift by the front lower support.
!

Chapter 3: Getting Started
Product Service 1-800-522-7658
7
Unpacking the Enclosure
Carefully remove the shrink-wrap or carton on the enclosure and inspect it for
damage that may have occurred in transit. If damaged, notify the delivery carrier
immediately and retain the entire shipment intact for inspection by the carrier.
DO NOT RETURN GOODS WITHOUT THE PRIOR
AUTHORIZATION OF LABCONCO. UNAUTHORIZED
RETURNS WILL NOT BE ACCEPTED.
IF ENCLOSURE WAS DAMAGED IN TRANSIT, YOU MUST
FILE A CLAIM DIRECTLY WITH THE FREIGHT CARRIER.
LABCONCO CORPORATION AND ITS DEALERS ARE NOT
RESPONSIBLE FOR SHIPPING DAMAGES.
Do not discard the packing material until you have checked all of the components
and tested the enclosure. We recommend that you do not remove the enclosure
from its shipping container until it is ready to be placed into its final location.
Move the unit by using a floor jack.
Do not move the enclosure by tilting it onto a hand truck.
Installing the Enclosure on a Supporting
Structure and Work Surface
When installing the enclosure onto a chemically-resistant work surface or
benchtop, ensure that the structure can safely support the combined weight of the
enclosure and any related equipment. The work surface should be at least as wide
and deep as the non-ventilated enclosure to properly support it. The front of the
non-ventilated enclosure should be aligned within 1/8" of the front of the work
surface. Mounting holes are provided in the Labconco accessory work surfaces to
secure the enclosure.
Work Surface Specifications
The work surface should be smooth, rigid, and durable, such as a chemically-
resistant epoxy resin. The surface should be
non-porous and resistant to the materials used in conjunction with the Purifier PCR
or Purifier Tissue Culture Enclosures. The work surface should also contain a
dished recessed area for containing primary spills.
The United
States
Interstate
Commerce
Commission
rules require
that claims be
filed with the
delivery
carrier within
fifteen (15)
days of
delivery.
!



Chapter 3: Getting Started
Product Service 1-800-522-7658
8
8
Work Surface and Non-Ventilated Enclosure Installation
1. Level the base cabinets and the work surface. Work surface should be
placed flush with the front of the base cabinet as shown in Figure 3-1.
2. Position the work surface in its intended location and with the front of the
work surface, (wide flange), towards you.
3. Secure the work surface to the base cabinet with a structural adhesive or
silicone sealant.
4. Insert the supplied mounting screws in the four holes. Allow a minimum
of 1/8" clearance under the head of the screw for positioning the enclosure.
5. Place the enclosure on the work surface and slide the rear flange and lower
front bracket flanges under the mounting screw heads.
6. Tighten the four screws to complete the installation.
Figure 3-1
Side View of Typical Installation

Chapter 3: Getting Started
Product Service 1-800-522-7658
9
Connecting the Electrical Supply
A grounded receptacle should be nearby for connecting the Purifier PCR or
Purifier Tissue Culture Enclosures. Please review Chapter 2 for electrical
prerequisites.
All wiring for the building electrical outlets SHOULD be
performed by a licensed electrician and conform to all local
codes.
Do not use any detachable power cord that is not adequately
rated for the unit.
Ne pas utliser un fil électrique amovible qui n’est pas du tension
nominale de l’appareil.
Sealing the Enclosure to the Work
Surface
When the enclosure has been set in place, it may be sealed at the work surface to
prevent spilled materials from collecting under the walls. A bead of silicone
sealant is recommended to seal it to the work surface.
!

Product Service 1-800-522-7658
10
10
CHAPTER 4
FEATURES AND SAFETY
PRECAUTIONS
Features
Labconco engineered the Purifier Passive PCR Enclosure and Purifier Tissue
Culture Enclosure to provide a non-ventilated, circulation-free work area to
reduce cross-contamination in laboratory applications. A spacious interior and
convenience outlet give ample room for media preparation and equipment used for
procedures. A UV light with digital timer, included on the Purifier PCR
Enclosure, provides decontamination control after PCR protocols are conducted.
The Purifier Tissue Culture Enclosure may be used for techniques such as tissue
staining, culture growth and virus harvesting. A built-in UV light on the Purifier
Tissue Culture Enclosure provides secondary surface decontamination while the
enclosure is not in use.

Chapter 4: Features and Safety Precautions
Product Service 1-800-522-7658
11
1. Ergonomic Slope and Front of 20° provides maximum visibility, and
comfort, reduces glare, thereby minimizing operator fatigue.
2. Internal Depth of 23" and interior height of 22" provides necessary
depth and height to support procedures and other auxiliary equipment
without extending outside the non-ventilated enclosure.
3. Flush Sash has a wiping seal and features a spring-loaded latch for loading
auxiliary equipment. The sash pivots down for normal operation.
4. Electrical Pass-Through Iris allows electrical cords and data cords to pass
through the back of enclosure. The unit ships with solid plugs and the iris
plugs are included with the User’s Manual for your convenience.
5. Fluorescent Light provides illumination for the interior of the enclosure.
6. Ultraviolet Germicidal Light provides surface decontamination and
maintains sterility inside the enclosure. It is located above the work area.
7. Variable PCR Digital UV Light Timer (Purifier PCR Enclosure only)
allows the operator to time the surface disinfection of the work area for
PCR protocols. The UV light timer has eight settings of 5, 10, 15, 30, 60,
Figure 4-1
Features
5
7
3
8
9, Internal
6
4
2
1
10, Not
Shown
PCR

Chapter 4: Features and Safety Precautions
Product Service 1-800-522-7658
12
12
120, 240 minutes and infinitely ON. It is easily changed by selecting the
time necessary on the control panel.
8. UV Sash Closure Panel provides added safety to prevent access and air
currents inside the non-ventilated enclosure when the UV light is activated.
The panel must be in place for the UV light to operate.
9. Switched Outlet is located inside the non-ventilated enclosure for
powering electrical equipment.
10. Accessory Work Surface is dished to contain spills and is contoured to fit
the dimensions of the non-ventilated enclosures.
Safety Precautions
1. Use good housekeeping in the enclosure at all times. Clean up spills
immediately. Periodically clean all interior surfaces.
2. Do not work with hazardous volatile chemicals in this enclosure.
3. All acid use in this enclosure is prohibited.
4. Radioisotope materials are prohibited in this enclosure.
5. The use of safety goggles, protective clothing, gloves and any other
personal protective equipment recommended by your safety officer should
be used.
6. The sash should remain in the down position while using the enclosure.
7. Ensure the enclosure is connected to an electrical service in accordance
with local and national electrical codes. Failure to do so may create a fire
or electrical hazard. Do not remove or service any electrical components
without first disconnecting from electrical service.
8. Ensure only trained operators use the non-ventilated enclosure. All users
should review the User’s Manual and become familiar with the operation.
9. Avoid direct exposure to ultraviolet (UV) radiation; NEVER work in the
non-ventilated enclosure with the UV light on. Always use the UV sash
closure panel in the closed position when using the UV light to provide
added protection.

Product Service 1-800-522-7658
13
13
CHAPTER 5
USING YOUR ENCLOSURE
Planning
Thoroughly understand procedures and equipment required before
beginning work.
Arrange for minimal disruptions, such as room traffic or entry into the
room while the enclosure is in use.
Start-up
Clean and surface disinfect the inside of the enclosure. Use UV light as
required for protocol.
Turn on the fluorescent light.
Only raise the sash for loading.
Wear a long sleeved lab coat and rubber gloves. Use protective eyewear.
Wear a protective mask if appropriate. Consult your Safety Officer for
additional personal protective equipment recommendations.
Work Techniques
Keep all materials inside and perform all operations inside the work area.
Unloading Materials and Equipment
All open trays, weigh vessels and containers should be covered before
being removed from the enclosure.
Shutdown
Turn off the fluorescent light and install the UV sash closure panel.
Clean and surface disinfect the interior surfaces after completion.
Turn on the UV light to decontaminate the work area as determined by
your protocol.

Product Service 1-800-522-7658
14
14
CHAPTER 6
MAINTAINING YOUR
ENCLOSURE
Now that you have an understanding of how to work in the non-ventilated
enclosure, we will review the suggested maintenance schedule and common
service operations.
Routine Maintenance Schedule
Weekly
Clean the enclosure interior appropriate for the application.
Monthly (or more often as required)
Using a cloth and glass cleaner, clean the exterior surfaces of the non-
ventilated enclosure, particularly the front to remove any accumulated dust.
All weekly activities.
Annually
Replace the UV lamp.
Verify proper operation of the UV panel safety switch
All monthly activities.
UV Light Replacement
1. Disconnect power.
2. Locate the UV lamp inside.
3. Rotate and remove the old UV lamp.
4. Reinstall the new UV lamp.
5. Switch unit on and try the new UV lamp (UV Closure Panel must be
installed.

Chapter 6: Maintaining Your Enclosure
Product Service 1-800-522-7658
15
Fluorescent Light Replacement
1. Disconnect power.
2. Locate the two Phillips machine screws on top of the light and remove.
Lift up the light assembly.
3. Rotate and remove the old fluorescent lamp.
4. Reinstall the new fluorescent lamp, light and screws in reverse order.
5. Switch unit on and try the new fluorescent lamp.

Product Service 1-800-522-7658
16
16
CHAPTER 7
ACCESSORIZING YOUR
ENCLOSURE
There are several ways to accessorize the enclosure for your individual
requirements. These include the addition of accessory work surfaces, storage
cabinets, base stands and mobile stands.
1. Work Surfaces
An optional dished work surface is available.
Dished work surfaces are contoured to fit the enclosure to contain spills.
Epoxy is chemical resistant.
Catalog #
Description
Dimensions (W x D x H)
3908402
Black, 4-foot wide
48" x 26.66" x 1"
3908405
Gray, 4-foot wide
48" x 26.66" x 1"