Labnet Excel E10-1 User manual

LABNET Excel
Single and Multi-channel Electronic Pipettors
Operation Manual
Version: 2.9
www.Labnetlink.com

Labnet International
31 Mayfield Ave
Edison, NJ 08837
USA
Declaration of Conformity
Product Name: Labnet Excel Single/Multi-channel Electronic
Pipettors
All models comply with the following European standards:
EMC: EN 55011, Class A
EN 60601-1-2
To the best of my knowledge and belief, these units conform to these
standards.
Name: Mike Revesz
Position: Director of Quality Assurance
Issue Date: 2005/1/1

Table of Contents
1. Safety Precautions...................................................................................1
1.1. Operation Safety Precautions 1
1.2. Battery Safety 1
2. General Description................................................................................3
2.1. Features 3
2.2. Ranges and Specifications 4
3. Getting Started ........................................................................................4
3.1. Unpacking 4
3.2. Inserting the Battery 5
3.3. Charging the Battery 6
3.4. Start Pipetting 8
3.5. Manual Blow-out Operation 10
3.6. Hanger Installation 10
4. LABNET Excel electronic pipettor Overview.....................................11
4.1. Keypad Function 12
4.2. Audible Signals 13
5. Function Map ........................................................................................14
9. SYS (System) Mode...............................................................................15
6.1. Aspirating Speed 15
6.2. Dispensing Speed16
6.3. Audible Signals 16
6.4. Blow-out 16
6.5. Reverse Pipetting17
6.6. Setting PROG (Programs) 18
7. AUTO Mode ........................................................................................ 19

8. MD (Multiple Dispensing) Mode .........................................................21
9. MIX Mode.... 22
10. SE (Sequential Aspirating/Dispensing) Mode.....................................22
10.1. SA (Sequential Aspirating) Mode 23
10.2. SD (Sequential Dispensing) Mode 24
11. Sterilization.. 26
11.1. Removal of the Lower Piston Assembly 27
11.2. Reattach the lower piston assembly 28
12. Maintenance 30
13. Troubleshooting.....................................................................................31
Appendix A: Technical Specifications...........................................................33
Appendix B : Tip Ejector Height Adjustment..............................................33
Appendix C: Warranty...................................................................................34

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1. Safety Precautions
1.1. Operation Safety Precautions
When using infectious, radioactive, toxic and other solutions which
may pose health risks, please observe appropriate safety precautions.
1. Do not use the LABNET Excel electronic pipettor in a potentially
explosive environment or with potentially explosive chemicals.
2. When using organic solvents or corrosive chemicals, please check their
compatibility with the pipette tips and the LABNET electronic pipettor.
3. Repair should be carried out by LABNET’s authorized service
personnel only. Contact LABNET International, Inc. for information
on authorized service options.
4. Use original spare parts and accessories only.
1.2. Battery Safety
The LABNET Excel electronic pipettors operate on a small but
powerful Lithium-ion battery. Misuse or abuse of the Lithium-ion
battery may cause damage or injury through fire, electric shock, or
chemical leakage. Please read and understand all warnings before using
the battery.
1. When storing the battery, do not allow it to come into contact with any
metallic surfaces.
Before using the LABNET Excel electronic pipettor for the first time,
please read this entire operating manual carefully. To guarantee problem
free, safe operation, it is essential to observe the following points:

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2. Never use the LABNET Excel electronic pipettors while using the
DC-in jack to charge the battery.
3. Do not incinerate the Lithium battery or expose it to excessive heat.
4. Do not short-circuit, puncture, crush, disassemble, damage, force
over-discharge (reversal) or modify the battery.
5. Do not expose the battery to water or moisture.
6. Do not drop or subject the battery to strong impacts.
7. Only use the battery specified in this manual.
8. Only use the specified, battery charging unit or DC-in jack to charge
the battery.
9. Do not use a leaking battery.
10. If charging is not completed within the specified time period, unplug
the charger and discontinue charging immediately.
11. The charger and battery temperature rises with extended periods of use.
Care should be taken to avoid burns.
12. Burns may result if the battery is removed immediately after extended
periods of use.
13. If fluid from the battery enters your eye, immediately rinse the eye with
plenty of fresh water and contact a doctor. If fluid from the battery
makes contact with your skin or clothing, wash the area thoroughly
with water.

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2. General Description
2.1. Features
zHigh accuracy and precision
zLow forces
zUseful, practical operation protocols, such as Automatic Pipetting
(AUTO) with Mixing (MIX) option, Multiple Dispensing (MD),
Mixing (MIX) and Sequential Aspirating/Dispensing (SE), etc.
z9 user-defined program sets
z5 speeds for aspirating and dispensing
zLi-ion battery provides extended use on a single battery charge
zErgonomic design
zAutomatic calibration
zUser-friendly graphic operation interface.
zAdjustable tip ejector.
zAutomatically remembers the last-used pipetting protocol and settings
zComplies with CE, ISO-8655, GLP
LABNET Excel electronic pipettors feature an accurate and efficient, as
well as ergonomic and light-weight design. Researchers can program these
state-of-the-art products to conduct different pipetting tasks. More accurate
and comfortable pipetting can be easily achieved without any concern of
finger stress or injury.

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2.2. Ranges and Specifications
Product No. Channels Volume
Range (µl)
Increment
(µl)
Accuracy
(Rel.±)
Precision
( Rel. CV )
E10-1 1 0.5 -10 0.1
4.0-1.0% 2.5-0.4%
E20-1 1 2 -20 0.1
5.0-1.0% 2.0-0.3%
E200-1 1 10 -200 1 2.0-0.6% 1.0-0.15%
E1200-1 1 100 -1,200 1 1.5-0.5% 0.6-0.15%
E10-8 8 0.5-10 0.1 4.0-1.0% 2.5-0.4%
E20-8 8 2-20 0.1 5.0-1.0% 2.0-0.3%
E200-8 8 10-200 1 2.0-0.6% 1.0-0.15%
E1200-8 8 100 -1,200 1 1.5-0.5% 0.6-0.15%
Note:
LABNET Excel electronic pipette Accuracy and Precision Specifications
have been attained using LABNET Excel pipette tips. LABNET
guarantees Accuracy and Precision only if LABNET Excel tips or other
approved tips are used.
3. Getting Started
3.1. Unpacking
zSingle/8-channel LABNET
electronic pipette unit
zOperation manual
Open the LABNET Excel electronic pipettor package and confirm that all
items are included:

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zQuality assurance card zAC-DC power adaptor
zLABNET electronic pipette
hanger and accessories
zPipette tips
3.2. Inserting the Battery
Figure 1: Open the battery cover. Figure 2: Insert the battery.
If there are any items missing, damaged, or not according to your order,
please contact your distributor or sales representatives for replacement
immediately.
Please charge the battery before first-time operation.
Remove the battery cover by pressing the battery cover latch (see Figure 1)
and move the cover outward. Insert the battery (see Figure 2). Metallic
contacts must be face down. Close the cover of the battery compartment.
Press the RESET button to power on and calibrate the LABNET electronic
pipette.

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Note: After a charged battery is inserted, the LABNET electronic pipette
will automatically carry out a calibration routine.
3.3. Charging the Battery
Included AC-DC Power Adapter
Optional Charging Kit
There are two ways to charge the battery: (1) Attach the included AC-DC
Power Adapter to the pipettor (2) Use the optional battery charging kit.
Insert the battery into the battery compartment. Insert the DC-in plug of the
power adaptor into the DC-in jack (see Figure 3). Connect the power plug
of the power adaptor with the external power source. Connecting the power
plug before inserting the battery will inactivate the charging process. The
indication bar inside battery symbol will blink during the charging
process. When the battery is fully charged, the blinking will stop and the
LABNET electronic pipette will beep once to remind the user.
Connect the power plug of the charging unit to the proper external power
source. The indication light will turn green. Insert the battery (see Figure 4).
Once the battery is correctly inserted and charging, the indicator light will
turn red. When the battery capacity is full, the indicator light will turn green
again.

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Figure 3: Using the included AC-DC
adapter to charge the battery Figure 4: Using the optional charging kit to charge the battery
Note:
1. For safety reasons, the LABNET Excel electronic pipettors cannot be
used during charging.
2. Please do not over-charge the battery for a long period of time.

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3.4. Start Pipetting
There is no power On/Off button on the LABNET Excel electronic
pipettors. Once users insert the fully-charged battery, the pipettor will be
switched on. It will perform a calibration routine and switch on the LCD
display as in Figure 8. Press any keypad button or the PLUNGER button to
enter the function mode selection. The LABNET Excel electronic pipettor
will be automatically powered off if it’s not in use for more than 10
minutes.
Select the desired function mode and settings (refer to the following
chapters : 6 ~ 10 ) before pipetting. After the initial set-up, attach the
correct-sized tips to the cone(s) of the pipette before operation.
Product No. Channels Volume
Range (µl)
Correct
Pipette Tip
Size
SE10-1 1 0.5 -10 10µl
SE20-1 1 2 -20 10µl
SE200-1 1 10 -200 200µl
SE1200-1 1 100 -1,200 1000 µl
SE10-8 8 0.5 -10 10µl
SE20-8 8 2-20 10µl
SE200-8 8 10-200 200 µl
SE1200-8 8 100 -1,200 1000 µl

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Figure 5: Correct hand position
The cones of the LABNET Excel electronic pipettor are designed to fit a
wide variety of manufacturers pipette tips. However, the published
accuracy and precision specifications for the LABNET Excel electronic
pipettors are guaranteed only when using LABNET Excel tips. Contact
LABNET International for pipette tip ordering information.
The LABNET Excel electronic pipettor is an ergonomic instrument
designed to reduce hand fatigue and repetitive stress injury. In order to
maximize the benefits of the LABNET Excel electronic pipettor’s design,
the instrument should be held as shown in figure 5. Use your index finger
to press the PLUNGER button and use your thumb to press the TIP ejector.

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3.5. Manual Blow-out Operation
3.6. Hanger Installation
Figure 6: LABNET Excel electronic pipettor hanger installation
The automatic blow-out function, in different pipetting modes, simulates
the blow-out function in manual pipettes. With the Blow-out function
selected, the user can press the PLUNGER button once to dispense the
liquid and the piston will travel an extra distance to blow out any remaining
liquid. The piston will then pause and return to its starting position.
Alernatively the user can press and hold the PLUNGER button to dispense.
The piston will travel the extra distance to blow out remaining liquid and
remain in that position until the plunger is released. Only when the
PLUNGER button is released, the piston will return back to its starting
position.
The LABNET Excel electronic pipettor hanger can be attached to a shelf or
counter using the sticker as shown in Figure 6. The finger rest of the
LABNET Excel electronic pipettor locks into the hanger and keeps the
pipettor in a vertical position.

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4. LABNET Excel electronic pipettor Overview
Lower Part
(Front View)
Tip Fitting
Bushing
(Side View)
Connector Nut
Finger Rest
"RESET"Button
Tip Ejector
Keypad
LCD Display
"PLUNGER" Button
DC In
BatteryCover
Figure 7: 1-channel LABNET Excel electronic pipettor overview
This section presents an overview of the LABNET Excel electronic
pipettor’s various components and buttons (see Figure 7) as well as the
symbols and indicators on the LCD display (see Figure 8).

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"Back" Bu tton
Sequential Aspirating/ Dispensing
Multiple Dispensing
"RESET" Button
Battery Capacity
Direction
Speed
Mixing
Auto Pipetting
System Setting
"Up / D own " Button
"En ter" Button
Reverse Pipetting
Volume ( )
Blow Out
Au d ible S in ga ls
Count No. / Prog No.
Figure 8: LCD display Panel
4.1. Keypad Function
Button Symbol Function Description
Set/Enter Confirms your choice
Exit/Esc Exits to previous mode/setting
Up Increases volume/ changes parameter
Down Decreases volume/ changes parameter
Reset RESET Piston automatically determines the
home position
PLUNGER PLUNGER Activates aspirating/dispensing/
same as button
Tip Ejector TIP Ejects the tips

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Tips for Efficient Operation: The PLUNGER button can be used to increase
volumes and to change modes and settings same as the “Up” button during
the function mode selection and volume settings. This operation feature can
speed up the setting processes.
4.2. Audible Signals
Audible Sound Operation
Low-tone beep Aspirating
High-tone beep Dispensing/ Tip Ejection/ Error codes
2 beeps MD/MIX/SE mode cycle or RESET routine is
completed
The audible signals are beneficial when you are familiarizing yourself with
the operating procedure of the LABNET Excel electronic pipettor. They can
also be switched off if desired (see Section 6.3.)

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5. Function Map
Figure 9: Function map
The main menu of the LABNET Excel electronic pipettor includes five
function modes (SYS, AUTO, MD, MIX and SE). Each mode includes
various function settings (see Figure 9).
Function Map
SYS AUTO MD MIX SE
Function
Mode
Aspirating
Speed Dispensing
Number
(COUNT)
Basic
Aspirating/
Dispensing
Mix
Number
(
COUNT
)
Dispensing
Speed
Audible
Signals
Program
Recall
Reverse
Pipetting
Tip Ejection
Alarm
Blow-out
Dispensing
Volume Mix Volume
SDSA
Aspirating
Number
(1,2,3,…)
Dispensing
Number
(1,2,3,…)
Aspirating
Volume
Dispensing
Volume
Function
Settings
MIX
Mix
Number
(
COUNT
)
Mix
Volume

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9. SYS (System) Mode
Note :
1. These 6 function settings are sequential procedures. To store these
settings in memory, users have to complete the whole setting procedure
and press «SET» when the LCD displays .
2. Users can use the «Up/Down» button to move back and forth to the
previous function settings.
6.1. Aspirating Speed
1. Press «EXIT» to enter the function mode selection. Then press
«Up/Down» to select the symbol (flashing).
2. Press «SET» to confirm the selection of mode and the
aspirating speed symbol will start flashing.
3. Press «Up/Down» to change the aspirating speed ( : Slowest, :
Fastest).
4. Press «SET» to confirm the speed selection and move to the
“Dispensing Speed” function setting.
SYS mode is used to set up the function settings before pipetting. Once
users select and store these settings, they will no longer need to set up the
settings before every pipetting. There are 6 function settings (Aspirating
Speed, Dispensing Speed, Audible Signals, Blow-out, Reverse Pipetting
and Program Recall) in SYS mode.
There are 5 speeds available for both aspirating and dispensing:

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6.2. Dispensing Speed
1. The “Dispensing Speed” symbol should now be flashing.
2. Press «Up/Down» to change the aspirating speed ( : Slowest, :
Fastest).
3. Press «SET» to confirm the speed selection and move to the
“Audible Signals” function setting.
6.3. Audible Signals
1. The “Audible Signals” symbol should now be flashing.
2. Press «Up/Down» to select the preference ( : On, : Off).
3. Press «SET» to confirm the selection and move to the “Tip
Ejection Alarm” setting.
6.4. Blow-out
1. The “Blow-out” symbol should now be flashing.
2. Press «Up/Down» to select the preference ( : On, :
Off).
Please refer to Section 4.2 for the definition of “Audible Signals”.
The automatic blow-out function, in different pipetting modes, simulates
the blow-out function in manual pipettes. To avoid the leftover final droplet
in tips, the blow-out setting is recommended in all protocols. The blow-out
setting will not be performed in “Reverse Pipetting” setting.