Labnet BioPette A Series User manual

Labnet BioPette A
Single-channel, 8-channel,
and 12-channel Pipettors
Instruction Manual
This manual is available in additional languages
at www.labnetlink.com.

Table of Contents
1. Product Description ............................................................. 1
2. Packing........................................................................................ 2
3. Pipettor Design........................................................................ 2
4. Safety Recommendations .................................................. 3
 5. Specications........................................................................... 4
6. Pipettor Operation................................................................. 6
7. Operating Instructions ......................................................10
8. Aspiration and Dispensing Instructions.....................11
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration................................................13
10. Pipettor Maintenance .......................................................16
11. Troubleshooting ...................................................................18
12. Spare Parts ..............................................................................20
13. Limited Warranty..................................................................23

Instruction Manual – 1
1. Product Description
The Labnet BioPette A single-channel pipettor is a volumetric
instrument designed to measure and transfer liquids
precisely and safely. It is available in volumes from 0.1 μL
to 10,000 μL.
The Labnet BioPette A 8-channel and 12-channel pipettors
aredesignedformicroplatelling.Thepipettorsenable
precise and simultaneous delivery of 8 or 12 preset
volumes of liquid. These pipettors are available in four
volume ranges: 0.5-10 μL, 5-50 μL, 20-200 μL, and 50-300 μL.
Nominal
Volume (µL) Cat. No.
Range of Pipettor
Volumes (µL)
Single-channel Pipettors
2 P3960-2A 0.1 - 2
10 P3960-10A 0.5 - 10
20 P3960-20A 2 - 20
100 P3960-100A 10 - 100
200 P3960-200A 20 - 200
1,000 P3960-1000A 100 - 1,000
5,000 P3960-5000A 1,000 - 5,000
10,000 P3960-10000A 1,000 - 10,000
8-channel and 12-channel Pipettors
8-10
12-10
P4608-10A
P4612-10A 0.5 - 10
8-50
12-50
P4608-50A
P4612-50A 5 - 50
8-200
12-200
P4608-200A
P4612-200A 20 - 200
8-300
12-300
P4608-300A
P4612-300A 50 - 300
Labnet pipettors operate using an air-cushion (i.e., the
aspirated liquid does not come into contact with the
shaft or plunger of the pipettor). The liquid is drawn into
the disposable tip attached to the pipettor.

2 – LABNET BIOPETTE A PIPETTORS
2. Packing
The pipettors are delivered with the following:
Description Qty/Pk
Instruction manual 1
QualityControlcerticate 1
Calibration key 1
Identicationlabels 8
Lubricant (for single-channel pipettor) 1
Ejector spacers (for models up to P3960-1000A) 2
Ejector cap (for P3960-5000A and P3960-10000A models) 1
Filters (for P3960-5000A and P3960-10000A models) 3
Filters
The 5,000 and 10,000 μL pipettors include a replaceable
lter,ttedatthebottomoftheshaftwhichprevents
aspirated liquid from entering the shaft and contaminating
theinnersurfaceandplunger.Useofthelterisparticularly
important when aspirating and dispensing large volumes
ofliquid.Theltershouldbereplacedifitbecomeswet.
3. Pipettor Design
Single- and Multi-channel Pipettors
N
A1
A2
B
C
D
R
F

Instruction Manual – 3
A. Pipetting pushbutton: Consists of 2 parts:
the pushbutton (A1) and the knob (A2).
B. Volume adjustment knob: Used to set the volume.
C. Shaft: Manufactured using high quality plastic,
ensuring high chemical and mechanical strength.
D. Tip ejector: For multi-channel pipettors, the tips are
ejected sequentially, reducing the required force.
F. Shaft nut
N. Ejector pushbutton
R. Multi-channel manifold: Contains a set of plungers
andasetofexible-suspensionshafts,whichfunction
to reduce the force required to attach the tip.
Model Identication
The volume range of the pipettor is shown on the
pipetting pushbutton.
4. Safety Recommendations
Long-term use of the pipettor relies on correct method of use.
Please read and follow the instructions for use carefully.
Symbols used:
Danger, risk of injury.
NOTE Risk of damage to the pipettor or errors in pipetting.
NOTE:
• The pipettor is designed for the transfer of liquids only
using the tip. Do not aspirate liquids without the tip
attached. The aspirated liquid should not enter the
pipettor, as it may cause damage.
• Single-use tips reduce the risk of contamination of
samples.
• Keep the pipettor clean, avoiding the use of abrasive or
corrosive cleaning agents (e.g., acetone).
• Keep the pipettor upright when there is liquid in the tip.
• Only using the pipettor in accordance with the
manufacturer’s instructions ensures the correct pipettor
parameters are maintained.

4 – LABNET BIOPETTE A PIPETTORS
• After replacing the plunger or the shaft, the pipettor
should be calibrated.
• In the case of incorrect operation, the device should
be cleaned in accordance with the Instruction Manual
or transferred to a service point.
• Ambient operating temperature is +5°C to 45°C.
• Ambient storage conditions (in the original packaging
during transport and short storage) is -25°C to 55°C.
When working with the pipettor:
• Follow general work safety regulations regarding
hazards related to work in the laboratory.
• Take special care when pipetting aggressive substances.
• Use appropriate protective attire (e.g., clothing, goggles,
and gloves).
• Avoid pointing the pipettor at yourself or others during use.
• Only use parts and accessories recommended by the
manufacturer.
5. Specications
The pipettor is a high quality instrument which offers
excellentaccuracyandprecision.Theaccuracyandprecision
(repeatability) of the liquid volume depends on the quality
of pipet tips used. The values for accuracy and precision
shown in the table below were obtained using manufacturer
non-lterpipettips.Thosetipsarerecommendedforuseto
ensure compatibility, accuracy and precision when pipetting.

Instruction Manual – 5
Nominal
Volume
(µL)
Volume
(µL)
Accuracy
(%)
Precision
(%)
Non-lter
Tips (µL)
Single-channel Pipettors
2
Min. 0.1 ±40.0 ≤12.0
10
0.2 ±12.0 ≤6.0
1 ±2.7 ≤1.3
Max. 2 ±1.5 ≤0.7
10
Min. 0.5 ±4.0 ≤2.8
10
1.0 ±2.5 ≤1.8
5.0 ±1.0 ≤0.6
Max. 10.0 ±0.5 ≤0.4
20
Min. 2 ±3.0 ≤1.5
20010 ±1.0 ≤0.5
Max. 20 ±0.8 ≤0.3
100
Min. 10 ±1.6 ≤0.80
20050 ±0.8 ≤0.24
Max. 100 ±0.8 ≤0.20
200
Min. 20 ±1.2 ≤0.60
200100 ±0.8 ≤0.25
Max. 200 ±0.6 ≤0.20
1,000
Min. 100 ±1.6 ≤0.40
1,000500 ±0.7 ≤0.20
Max. 1,000 ±0.6 ≤0.15
5,000
Min. 1,000 ±0.6 ≤0.25
5,0002,500 ±0.6 ≤0.20
Max. 5,000 ±0.5 ≤0.15
10,000
Min. 1,000 ±2.5 ≤0.6
10,0005,000 ±0.8 ≤0.3
Max. 10,000 ±0.5 ≤0.2
8-channel and 12-channel Pipettors
8 - 10
12 - 10
Min. 0.5 ±10.0 ≤8.0
10
1 ±8.0 ≤6.0
5 ±4.0 ≤2.0
Max. 10 ±2.0 ≤1.2
8 - 50
12 - 50
Min. 5 ±4.0 ≤2.5
20025 ±3.0 ≤1.2
Max. 50 ±1.6 ≤0.6
8 - 200
12 - 200
Min. 20 ±3.0 ≤1.5
200100 ±1.5 ≤0.8
Max. 200 ±1.0 ≤0.6
8 - 300
12 - 300
Min. 50 ±1.6 ≤1.5
300150 ±1.2 ≤1.0
Max. 300 ±1.0 ≤0.6

6 – LABNET BIOPETTE A PIPETTORS
The accuracy and precision were obtained gravimetrically
using manufacturer tips performing at least 10 measurements
of distilled water at a temperature of 20°C ± 1°C according to
EN ISO 8655 standards.
Theuseoftipsfromothermanufacturersorltertipsmay
result in incorrect liquid aspiration and require pipettor
recalibration.
The design of the pipettor enables the user to recalibrate it
according to the information presented in Section 9.
6. Pipettor Operation
Attaching the Tips
• Attach the correct tip corresponding to the model number
displayed below counter window (Section 5).
• Position the pipettor vertically when attaching tips.
• Single-channelpipettors:Pushthepipettiponrmly
using a slight twisting motion to ensure an airtight seal.
• Multi-channel pipettors: Press the pipettor against
thetipspositionedintherackboxuntiltheshaft
retractsapproximately1.5mmintothemanifold.The
suspension system ensures even and airtight sealing of
the pipet tips. The rocking motion does not have to be
performed to seal the pipet tips tightly.
✓✕ ✓✕
NOTE:
• Do not attach the tips with a rocking movement,
as this may damage the shaft or plunger. Observe this
rule particularly with single-channel pipettors of low
volume range.
• Never draw liquids directly into the pipettor without
the tip attached.

Instruction Manual – 7
Adjusting the tip ejector
The tip ejector can be adjusted by the user and can
accommodate most types of tips available on the market.
When using narrow tubes, it may be necessary to remove
the tip ejector.
2-1,000 µL pipettors
1. Tip ejector disassembly
• Press the tip ejector
pushbutton.
• Slide the ejector from the
arbor.
2. Tip ejector assembly
• Press the tip ejector
pushbutton.
• Line the metal tip ejector up
with the arbor.
• Slide the ejector on the
arbor.
3. Tip ejector adjustment
• Slide the ejector from the arbor.
• Remove “H0” spacer that is inserted on the ejector
arbor. To increase the ejector length use ”H” spacers,
which allow for regulating the length of tip ejector
by +1 mm (H1) or +2 mm (H2). The outside shape
identiesthespacers.
4

8 – LABNET BIOPETTE A PIPETTORS
5,000 and 10,000 µL pipettors
1. Tip ejector disassembly
• Remove the tip ejector pushbutton.
• Using a screwdriver, turn the metal arbor counter-
clockwise to unscrew the ejector from the arbor.
• Release the ejector.
2. Tip ejector assembly
• Remove the tip ejector pushbutton.
• Using a screwdriver, turn the metal arbor clockwise
to secure the ejector and adjust its length.
NOTE: Leave a space of at least 1 mm between the
shaft and tip ejector collar.
3. Tip ejector adjustment
• Remove the ejector pushbutton.
• Use the screwdriver to increase or decrease the
ejector length.
• Following ejector assembly or adjustment, ensure
the ejector pushbutton is relocated.
Ifthetipejectorcannotbeadjustedsufciently,orifthe
diameteroftheejectorisinsufcienttoejectthetip,it
may be necessary to attach the ejector cap “M”to the
ejector.