5
Contents
Contents
1. Important Notes about this Manual ......... 7
1.1 Text Symbols, Pictograms and their
Meanings....................................................... 7
2. Intended Purpose of the Microscope....... 9
3. Safety Notes ............................................... 10
3.1 General Safety Notes................................ 10
3.2 Electrical Safety ......................................... 11
3.3 Transport and Storage............................... 12
3.4 Notes on Handling Light Sources............ 12
3.5 Notes on Handling Immersion Oil............ 12
3.6 Notes on Handling Acids and Bases...... 13
3.7 Disposal ...................................................... 13
3.8 Type Labels ................................................. 13
4. Overview of the Instrument ..................... 14
5. Unpacking the Microscope ..................... 19
6. Assembling the Microscope ................... 21
6.1 Stage .......................................................... 21
6.2 Condenser................................................... 23
6.3 Incident Light Axis ..................................... 24
6.4 Tube and Eyepieces................................... 24
6.5 Objectives.................................................... 24
6.6 Illumination.................................................. 25
6.6.1 LED Lamp Housing LH113................ 25
6.6.2 Lamp Housing 107/2*........................ 26
6.6.3 Lamp Housing 106z*......................... 27
6.7 Equipping the Incident Light Turret Disk 30
6.8 Analyzer and Polarizer.............................. 31
6.9 Lambda Plate Compensator*.................. 31
6.10 ICR/ICT Prisms............................................ 31
6.11 Optional Accessories ................................ 32
6.12 Connection to the Power Supply............. 34
7. Startup ......................................................... 35
7.1 Switching On............................................... 35
7.2 Köhler Illumination..................................... 35
7.2.1 Transmitted Light........................................ 35
7.2.2 Incident Light .............................................. 37
7.3 Checking Phase Contrast Rings .............. 38
7.4 Adjustment of Condenser Prisms............ 39
7.5 Adjusting the Light Sources
(106z Lamp Housing only) ......................... 41
8. Operation..................................................... 45
8.1 Switching On............................................... 45
8.2 Stages and Object Displacement............ 45
8.3 Focusing ...................................................... 46
8.4 Tubes48
8.5 Eyepieces .................................................... 49
8.6 Objectives.................................................... 50
8.7 Light Sources.............................................. 51
8.8 Aperture Diaphragm.................................. 51
8.9 Field Diaphragm (Transmitted Light)....... 53
9. Contrast Methods ...................................... 54
9.1 Incident Light .............................................. 54
9.1.1 Bright Field (RL)................................. 54
9.1.2 Oblique Illumination (RL).................. 54
9.1.3 Polarization (RL) ................................ 55
9.1.4 Interference Contrast ICR ............... 55
9.1.5 Dark Field (RL) ................................... 56
9.1.6 Fluorescence..................................... 56
9.2 Transmitted Light........................................ 57
9.2.1 Bright Field (TL)................................. 58
9.2.2 Phase Contrast (TL).......................... 59
9.2.3 Dark Field (TL).................................... 59
9.2.4 Oblique Illumination (TL).................. 60
9.2.5 Polarization (TL) ................................ 60
9.2.6 Differential Interference
Contrast ICT ....................................... 61
