Leica DMi1 User manual

Leica DMi1
Instructions for use
Gebrauchsanweisung
Mode d’emploi
MICROSYSTEMS
Leica Microsystems CMS GmbH. Handbuch, 11934049, Revision 1.0, 2014-04-15

Revision 1.0, published 2014-04-15 by:
Revision 1.0, veröffentlicht 2014-04-15 von:
Édition révisée 1.1, Édition révisée 1.0 2014-04-15 par:
Leica Microsystems CMS GmbH
Ernst-Leitz-Straße 17-37
D-35578 Wetzlar
http://www.leica-microsystems.com
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Marketing CMS

Leica DMi1
Instructions for use
MICROSYSTEMS
Leica Microsystems CMS GmbH. Instructions for use, 11934049, Revision 1.0, 2014-04-15

4
Copyrights
Copyrights
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduc-
tion of text or illustrations (in whole or in part)
by print, photocopy, microfilm or other method
(including electronic systems) is not allowed
without express written permission from Leica
Microsystems CMS GmbH.
The term Windows may appear in the following
text without further identification. It is, however,
a registered trademark of Microsoft Corpora-
tion. The names of companies and products used
herein may be trademarks of their respective
owners.
The instructions contained in the following docu-
mentation reflect state-of-the-art technology. We
have compiled the texts and illustrations as ac-
curately as possible. Still, we are always grateful
for comments and suggestions regarding poten-
tial mistakes within this documentation.
The information in this instructions for use is sub-
ject to modification at any time and without noti-
fication.

5
Contents
Contents
1. Important Notes about this
Instructions for use ..................................... 6
2. Intended Purpose of Microscopes........... 8
3. Safety Notes ............................................... 10
3.1 General Safety Notes................................ 10
3.2 Electrical Safety......................................... 11
3.3 Type Labels ................................................. 12
3.4 Notes on Handling Light Sources............ 13
3.5 Notes on handling immersion oil............. 13
3.6 Notes on handling acids and bases........ 13
3.7 Disposal....................................................... 13
4. Overview of the Instrument ..................... 14
5. Unpacking................................................... 21
6. Assembling the Microscope ................... 23
6.1 Attaching the condensers........................ 23
6.2 Adjusting the transmitted light
illumination column.................................... 23
6.3 Eyepieces and graticules* ...................... 24
6.4 Objectives.................................................... 25
6.5 Inserting the filter....................................... 25
6.6 Object guide................................................ 25
6.7 Light source for the transmitted
light axis....................................................... 26
6.8 Electrical connection of the microscope26
7. Assembling the Options ........................... 27
7.1 Inserting the phase contrast slider on the
transmitted light illumination carrier* .... 27
7.2 Mount the camera system........................ 27
7.3 Mount the camera IR reflection shield... 28
8. Operation..................................................... 29
8.1 Basic settings for transmitted light......... 29
8.2 Objectives.................................................... 31
8.3 Transmitted light......................................... 32
8.4 Phase contrast ........................................... 34
8.5 Camera ........................................................ 34
9. Trouble Shooting........................................ 35
10. Care of the Microscope............................ 37
10.1 Dust Cover................................................... 37
10.2 Cleaning....................................................... 37
12.3 Handling Acids and Bases ....................... 38
11. Technical Description .............................. 39
12. EU Declaration of Conformity.................. 44

6
1. Important Notes about this Instructions for use
Numbers in parentheses such as “(1.2)”, corre-
spond to illustrations (in the example, Figure 1,
Item 2).
Numbers with an arrow, e.g. →p. 20, refer to a
specific page of the manual.
WARNING indicates a hazard with a medium
degree of risk that, if not avoided, can result in
death or serious injury.
CAUTION indicates a hazard with a low degree
of risk that, if not avoided, can result in minor or
moderate injury.
Caution!
Special safety instructions within this In-
structions for use are indicated with the tri-
angle symbol shown here, and have a gray
background.
Notice! The macroscope and accessories can be
damaged when operated incorrectly.
Caution!
This Instructions for use is an essential com-
ponent of the product; it must be read care-
fully before the product is assembled, put into
operation or used, and must be kept for later
reference.
1. Important Notes about this
Instructions for use
This instructions for use contains important in-
structions and information for the operational
safety and maintenance of the microscope and
accessories.
Text symbols, pictograms and their meanings:
(1.2)
→p.20
!
WARNING
CAUTION

7
1. Important Notes about this Instructions for use
Warning of hazardous electrical voltage! Risk of
electrical shock!
Warning of optical radiation! Never look directly
into the light beam! Wear safety goggles!
Warning of electromagnetic field
Instructions for disposing of the instrument,
its accessories and consumables.
Connection to ground!
Explanatory note.
Item not contained in all configurations.
Device for in vitro diagnostics (IVD).
IVD manufacturing date, example 11 / 2011 for
November 2011.
China RoHS 50 years EFUP
(Environmentally friendly use period)
*
MM/YYYY
IVD

8
2. Intended Purpose of Microscopes
2. Intended Purpose of Microscopes
The Leica DMi1 microscope is an inverted light
microscope and is intended for use as a general
laboratory microscope suitable for routine exam-
inations of biological samples.
It is also designed and approved for applications
in industrial laboratory environment.
Indications for use
Inspection, counting, classifying, identifying and
controlling cell and tissue cultures, Reviewing
biological samples, liquids and sediments result-
ing in information regarding the physiological
and/or pathological conditions inborn anomalies,
monitoring therapeutic measures.
Any resulting actions are based on the interpre-
tation by the clinician.
Contra indications
Not suitable for examining potentially infectious
specimens.
Three basic microscope stands are available
for biological applications. One is the standard
bright field version with fixed eyepieces us-
ing the bright field (BF), phase contrast (Phaco)
methods. One is the standard bright field version
with exchangeable eyepieces using the bright
field (BF), phase contrast (Phaco) methods. The
other is the camera stand with exchangeable
eyepieces, which also offers camera module
in addition to the two transmitted light contrast
methods.
All microscopy methods and the necessary ac-
cessories for the Leica DMi1 are described and
explained in the operation section of this instruc-
tions for use, including their function and opera-
tion.
Directives of European Community (EC-Direc-
tives)
The named microscope complies with and is de-
clared according to the Council Directive 98/79/
EC concerning in vitro diagnostics.
It also meets the requirements of the Council
Directives 2006/95/EC concerning electrical ap-
paratus and 2004/108/EC concerning electromag-
netic compatibility.

9
2. Intended Purpose of Microscopes
Reasonably foreseeable misuse
The following are prohibited:
• To use the microscope for any purpose not in
accordance with the declaration of conformity.
• To use combinations with non-Leica compo-
nents that go beyond the scope of this manual.
• For unauthorized personnel to open the instru-
ments.
• To use cables that Leica has not provided or
permitted.
• To clean the microscope in a way other than
specified in the manual.
• To operate the microscope in a slope position.
• To use the illumination arm as handle to carry
the microscope.
• To use the camera with wrong size camera
chip
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscopes for other purposes
than those for which they are intended or not
using them within the specifications of Leica
Microsystems CMS GmbH.
In such cases the conformity declaration
shall cease to be valid.
Caution!
This (IVD) instrument is not intended for use
in a patient environment and not intended to
be combined with medical devices as defined
by EN 60601-1. If a microscope is electrically
connected to a medical instrument in accor-
dance with EN 60601-1, the requirements de-
fined in EN 60601-1-1 shall apply.
Not suitable for examining potentially infec-
tious specimens.
WARNING
Notes on handling laser devices
This microscope is not suitable for coupling
laser radiation (such as to the camera ports),
since this radiation poses a hazard for the
user (eye injury in particular).
Laser couplings require corresponding safety
devices that have to be inspected and in-
stalled by trained personnel.
For further information, please contact your
authorized Leica Microsystems representa-
tive.

10
3. Safety Notes
3. Safety Notes
3.1 General Safety Notes
This product of protection class 1 is built and
inspected in accordance with IEC / EN61010-1 -
Safety requirements for electrical equipment for
measurement, control and laboratory use - and
IEC / EN 61010-2-101
It also meets the EMC-standards IEC / EN 61326-1
and IEC / EN 61326-2-6 for electrical equipment
for measurement, control and laboratory use and
particular requirements for IVD-Medical Devices
and corresponds to the CE Directives.
It fulfills IEC / EN 62471, Photobiological safety of
lamps and lamp systems. This product is classi-
fied according to this into the exempt level (no
risk).
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
instructions for use.
Caution!
The devices and accessories described in
this instructions for use have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar must be consulted whenever
the device is altered, modified or used in con-
junction with non-Leica components that are
outside of the scope of this instructions for
use .
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims and product liability!

11
3. Safety Notes
3.2 Electrical Safety
General specifications
Microscope
For indoor use only.
Input:
Supply voltage: 100-240V AC
Max. Power 18VA
Frequency: 50/60Hz
Fuses: 5x20, T1.6A H 250 VAC
Output:
Output voltage: 5V DC
Max. Power: 10W
Ambient temperature: 15-35°C
Relative humidity: max 80% to 30°C
Overvoltage category: II
Pollution degree: 2
WARNING
Use only original power cables or alterna-
tive cables with a VDE / HAR logo, which at
least fulfill the requirement of 3x0.75 mm2and
10A/250V.
Caution!
Plugging or unplugging of cables is to take
place in the de-energized state!
WARNING
By definition, the main circuit breaker of this
instrument is the connection between the
power cable and device port. The user must
ensure unobstructed access to the main cir-
cuit breaker at all times.
WARNING
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
Caution!
Do not use this instrument near sources of
high electromagnetic radiation (for example,
unshielded, intentionally operated ultra-high
frequency sources), because these can dis-
rupt proper operation.
We recommend assessing the electromag-
netic environment before operation of this
instrument and then giving corresponding
instructions.

12
3. Safety Notes
Caution!
Do not use the microscope in altitudes ex-
ceeding 2000 m ASL/NL.
Caution!
Transport and storageinarange of-20° – +85°C
and at a humidity not exceeding 90%.
Caution!
The microscope’s electrical accessory com-
ponents are not protected against water. Wa-
ter can cause electric shock.
Caution!
Protect the microscope from excessive tem-
perature fluctuations. Such fluctuations can
lead to the accumulation of condensation,
which can damage the electrical and optical
components.
Ambient temperature: 15-35°C.
3.3 Type Labels
Fig. 1 Type label – 11526200
Leica DMi1 with fixed eyepieces
Fig. 2 Type label – 11526201
Leica DMi1 without eyepieces
Fig. 3 Type label – 11526203
Leica DMi1 without eyepieces with MC170 HD
Fig. 4 Type label – 11526206
Leica DMi1 without eyepieces with MC120 HD

13
3. Safety Notes
3.4 Notes on Handling Light Sources
CAUTION
Light sources pose a potential irradiation risk,
therefore never look directly into the light
beam!
3.5 Notes on handling immersion oil
Caution!
When using immersion oil, take care to avoid
skin contact! Ask the supplier for a safety
data sheet!
3.6 Notes on handling acids and bases
For examinations using acids or other aggressive
chemicals, particular caution must be taken.
Caution!
Be absolutely certain to avoid direct contact
with these chemicals.
3.7 Disposal
After the end of the product’s life, please contact
Leica Service or Leica Sales on how to dispose
of it.
Please observe the national laws and ordinances
which, for example, implement and ensure com-
pliance with EU directive WEEE.
Note!
Like all electronic instruments, the micro-
scope, its components and expendables may
not be disposed of as general household
waste!

14
4. Overview of the Instrument
4. Overview of the Instrument
Specification Leica DMi1
Contrast methods • Transmitted light: Bright field, phase contrast
Optics • Infinity corrected (HCS)
Transmitted light axis Integrated LED Illumination manual adjustment of
• Brightness
• Aperture diaphragm
• Automatic shutoff (adjustable)
Tube optionally with
• Fixed eyepieces FOV 18 tube (ordered together with stand)
• Exchangeable eyepieces FOV 20 tube (ordered together with stand)
Objective nosepiece • Manual
• 4-fold for objectives with M25 thread
Stage • Fixed stage (L*W) 262 x 212 mm
• Option: Attachable object guide for fixed stage
Condensers optionally with
• S80/0.30 condenser
• S40/0.45 condenser
Phase contrast slider optionally with
• 2 position slider (BF, Ph1) for S40
• 2 position slider (BF, Ph1) for S80
• 4 position slider (BF, Ph0,Ph1,Ph2) for S40
• 4 position slider (BF, Ph0,Ph1,Ph2) for S80
Focus • Focus dial for coarse and fine focusing
• Height adjustment
• Stroke distance is 9mm
• Min. Adjustment 2 µm
Camera optionally with
• Leica MC120 HD (2.5 M pixel) or MC170 HD (5 M pixel) camera
• HDMI port
• SD card
• Independent On/off button for Camera

15
4. Overview of the Instrument
Fig. 5 Right side of the Leica DMi1 stand
1 Binocular tube upper part
2 Eyepieces
3 Eyepiece tube
4 Tube Neck
5 Main power switch
6 Phase contrast slider insert slot
7 Integrated LED lamp housing
Important assembly groups
The following overall views illustrate and list im-
portant assembly groups of the microscopes and
its accessory components.
8 Insert for filter, Ø 43 mm
9 Condenser
10 Transmitted light illumination arm
11 Stop screw for condenser height adjustment
12 Coarse and fine focusing
13 Objective nosepiece and objectives
14 Camera protection shield
15 Camera
1
2
3
4
12
13
14
15
6
9
8
7
10
11
5

16
4. Overview of the Instrument
Fig. 6 Left side of the Leica DMi1 stand
1 Brightness control
2 Integrated LED lamp housing
3 Aperture diaphragm
4 Condenser
5 Coarse and fine focusing
6 Fixed stage
7 Stage insert
8 Object guide
1
5
6
87
2
3
4

17
4. Overview of the Instrument
Fig. 7 Front view of the Leica DMi1 stand
1 Binocular tube upper part
2 Eyepieces
Fig. 8 Rear view of the Leica DMi1 stand
1 Integrated C-Mount
2 Power supply connection
3 Connection to earth/ground!
4 Camera USB power supply port
5 Identification label
6 Camera protection shield fix points
1
2 2
2
3
1
4
5 6

18
4. Overview of the Instrument
Stand
The Leica DMi1 stand offers high stability due to
the low center of gravity. It offers solid design
and simple ergo controls
Tube Neck
The tube neck is the interface between the
microscope stand and the tube.
Tube
The tube contains a 1x tube lens which creates
the primary image in conjunction with the objec-
tive.
The tube for the Leica DMi1 comes together with
the stand. Tubes cannot be changeable.
Eyepieces
The eyepiece creates a magnified, virtual image
of the real intermediate image that is projected
by the objective. In this process, the eyepiece
works as a magnifying lens.
Brightness controller
The stand has built-in electronics for adjusting
the brightness via the brightness controller.
Integrated C-mount*
The integrated C-mount is consist in the stand,
with 0.5x magnification, only suitable for ½" size
camera chip, if mount with other size camera will
cause the problem.
Main power switch
The illuminated main power switch is used to
switch the power supply of the microscope on
and off. The illumination allows you to tell imme-
diately whether the microscope is switched on,
even in darkened rooms.
Transmitted light illumination unit
The transmitted light illumination unit consists
of the transmitted light illumination arm and the
transmitted light illumination column. The trans-
mitted light illumination column includes a pre-
centered, high-intensity LED illuminator, a fixture
for a diaphragm slide, a fixture for a light filter, a
condenser and an aperture diaphragm.
Filters
The green and yellow filter are generally used to
improve the contrast of the specimen. They are
firmly mounted in a spoon-shaped holder (Ø 43
mm).
Different filters can be inserted into the filter
holder of the transmitted light illumination unit.
The day light filter will change the color of the im-
age.
Addtionally, yellow filter Ø 43mm with round
mounting can be put in the BF position of slider,
only for change the color for BF image.

19
4. Overview of the Instrument
Aperture diaphragm
The aperture diaphragm defines the resolution,
focus depth and contrast of the microscopic im-
age. The best resolution is obtained when the
apertures of the objective and the condenser are
roughly the same.
Caution!
The aperture diaphragm in the illumination
light path is not for setting the image bright-
ness. Only the rotary brightness adjustment
knob or the neutral density filter should be
used for this purpose.
Condenser
The condenser is a lens system that collects the
light and directs it to the specimen from the top.
The condenser serves to utilize the numerical ap-
erture in the objective.
Stop screw for condenser height adjustment
The stop screw is used for fixing condenser
height adjustment by moving the transmitted light
illumination column. The markings on the trans-
mitted light column indicate the proper height to
be set for the condenser used.
For S40 condenser, the working distance can
be changed between 40mm and 50 mm. The
S80 condenser, has a fixed working distance of
80mm.
Specimen stages and accessories
The specimen stage is used to support the speci-
mens to be subjected to microscopic examina-
tion. For microscopy with the different speci-
mens, multiple options are available, such as the
attachable object guide and inserts.
Fig. 9 Insert of transmitted light filter
Fig. 10 Transmitted light illumination system

20
4. Overview of the Instrument
Objective nosepiece and objectives
The objective nosepiece is designed to hold the
objectives. Especially the L-type objectives with
long working distance are able to compensate
for the different thicknesses of flask bottoms.
All microscope objectives with magnifications
2.5x to 100x can be used. All objectives with a 25
mm thread are compatible. For additional infor-
mation about objectives, refer to the "Technical
Description" chapter or the respective objective
lists.
(www.leica-microsystems.com ->Products->
Light Microscopes->Accessories->Objectives)
Coarse and fine focus adjustment
The coarse and fine focus adjustment enables
you to adjust the microscopic image quickly and
accurately. The focus is adjusted by moving the
objective nosepiece vertically. The stroke length
9mm, minimum adjustment is 2 µm.
Object guide and inserts
Object guide is the accessory mounted on the
fixed stage, with the handle it can carries the
sample move in the X-Y direction. It offers differ-
ent inserts (holding frame) to hold different size
petri dishes, slides, multiwell plate or flasks etc.
Camera device*
The camera stand version with camera device
contains the integrated lens (Similar function as
C-mount, 0.5x).
Phase contrast slider**
The phase contrast slider is part of a contrast
method.
For the S40/0.45 and S80/0.30 condensers, four
different sliders are used for the phase contrast,
each condenser can use 2 different sliders. In
each case, there are one or three phase rings
are integrated on the sliders.
2 position slider contains one Brightfield (BF) and
one Ph1 phase ring, 4 position slider contains
one Brightfield (BF), one Ph0, one Ph1 and one
Ph2 phase ring
*Camera stands