Leica DM750 M User manual

Leica DM750 M
Manual

Leica DM750 M Manual 2
Table of Contents
Safety instructions 3
Safety Notes 4
Instructions on use 5
Instructions on use (continued) 6
Assembling the Leica DM750 M 7
Assembling the Incident Light Axis 8
Assembling the Viewing Tubes 9
Leica EZ Viewing Tube with Integrated Eyepieces 10
Leica EZ Viewing Tube – integrated eyepieces (cont.) 11
Leica Standard Tube with Separate Eyepieces 12
Eyecups 13
Installing Objectives 14
Conversion to Incident Light Polarization 15
Use 16
Switching on the Microscope 17
Modifying the Aperture Diaphragm 18
Illumination Scenarios 19
Illumination Scenarios (continued) 20
Using Specimen Holders 21
Automatic Shutoff of Illumination 22
Conversion and Use with Transmitted Light 23
Optional Koehler Illumination 24
Installing the Condenser 25
Installing the condenser (continued) 26
Switching on the Microscope 27
Complete Condenser Centering 28
Complete Condenser Centering (cont.) 29
Using the Condenser 30
Preparation for Viewing a Specimen Slide 31
Focusing 32
Viewing Tube Adjustment 33
Viewing Tube Adjustment (cont.) 34
Viewing Tube Adjustment (cont.) 35
Koehler Configuration 36
Koehler Configuration (cont.) 37
Automatic Shutoff of Illumination 38
Care and Repair Instructions 39
Care Instructions 40
Accessories, Maintenance and Repair 41
Specications and Dimensions 42
Electrical Data and Ambient Conditions 43
Dimensions (in mm) 44

Leica DM750 M Manual 3
Safety instructions

Leica DM750 M Manual 4
Before installing, operating or using the instrument, it is mandatory to read this
user manual. In particular, please observe all
safety instructions.
The "Safety Concept" brochure contains additi-
onal safety information regarding service work,
requirements and the handling of the micro-
scope, the electrical and other accessories as
well as general safety instructions.
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety instructions of the
supplier.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Warning of a danger
This symbol indicates especially impor- tant information that is mandatory to
read and observe. Failure to follow these inst-
ructions may pose hazards to personnel, cause
instrument malfunctions or damage the instru-
ment.
Hazardous electrical voltage
This symbol indicates especially impor- tant information that is mandatory to
read and observe. Failure to follow these inst-
ructions may pose hazards to personnel, cause
instrument malfunctions or damage the instru-
ment.
Important information
This symbol indicates additional infor- mation or explanations that intend to
provide clarity.
EC Declaration of Conformity
Electrically operated accessories are construc-
ted based on the state of the art of technology
and are provided with an EC Declaration of
Conformity.
Safety Notes
Contact address
Leica Microsystems (Schweiz) AG
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)

Leica DM750 M Manual 5
Intended use
The DM series microscopes by Leica are opti-
cal instruments for improving the visibility of
objects through magnification and illumina-
tion. They are used for observing and docu-
menting.
Place of use
Use the instrument in enclosed,
dust-free rooms at +10 °C to
+40 °C only. Protect it from oil, chemicals and
extreme humidity. It must be at least 10 cm
from the wall and away from flammable subs-
tances.
Interference
Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
images.
In warm and warm-damp climatic zones, the individual components require
special care in order to prevent the build-up of
fungus.
Use in clean rooms
Leica DM series microscopes can be used in
clean rooms without any problems.
Handling electrical components
Never install any other plug (NEMA 5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
The microscope must be connected to a grounded socket.
Always position the microscope so that you can disconnect it from the power
supply at any time. The power cable is provided
as the power disconnect device.
Instructions on use

Leica DM750 M Manual 6
Intervention from the user
The microscopes of the DM series contain no components that can be maintained
or repaired by the user. Exceptions are menti-
oned specifically in this manual.
Unless otherwise specified in this manual, repair and service work may
be carried out by authorized Leica technicians
only.
Unauthorized alterations to the instru- ment or noncompliant use shall void all
rights to any warranty claims.
Maintenance
Leica DM series microscopes are basi- cally maintenance-free. To ensure that it
always operates safely and reliably, we recom-
mend that you take the precaution of contac-
ting the responsible service organization. Here
you can arrange for periodic inspections and
conclude a maintenance contract (recommen-
ded).
Accessories
Only those accessories may be used that are
listed in this user manual or for which safe use
has been confirmed by Leica Microsystems.
Danger of infection
Direct contact with eyepieces is a poten- tial transmission method for bacterial
and viral infections of the eye. The risk can be
kept to a minimum by using personal eyepieces
for each individual or detachable eyecups.
Instructions on use (continued)

Leica DM750 M Manual 7
Assembling the Leica DM750 M

Leica DM750 M Manual 8
Assembly
1. Place the incident light axis with the heat
sink facing backwards onto the tripod
mount.
2. Align the incident light axis according to
the tripod geometry.
3. Fasten the incident light axis into place by
tightening the setscrew.
Assembling the Incident Light Axis

Leica DM750 M Manual 9
There are two types of viewing tube. Identify which viewing tube you have:
Type 1: Leica EZ Viewing Tube with integrated
eyepieces
Type 2: Standard tube with separate eyepieces
Assembly
1. Loosen the setscrew (on top of the stand)
using the Allen key provided.
2. Set the dovetail into the tripod mount and
tighten the setscrew carefully. This way,
the tube automatically shifts to the correct
position on the optical axis of the micro-
scope.
Assembling the Viewing Tubes

Leica DM750 M Manual 10
3. Reinstall the Leica EZ Viewing Tube onto
the stand.
4. Tighten the wingscrew using the wrench
included in the delivery package.
Leica EZ Viewing Tube with Integrated Eyepieces
Loosen the setscrew at the stand or replace the setscrew with the optional
wingscrew to rotate the Leica EZ Viewing Tube.
1. In order to use the wingscrew, remove the
setscrew that was delivered with the stand.
2. Detach the wing screw part completely
before the screw is used on the stand.
Leica EZ Viewing Tube with integrated eyepieces

Leica DM750 M Manual 11
Leica EZ Viewing Tube – integrated eyepieces (cont.)
Now, you can rotate the Leica EZ Viewing Tube by loosening the wingscrew, brin-
ging the tube to the desired position and tigh-
tening the wingscrew again.
The eyepieces are integrated into the Leica EZ Viewing Tube and preset; there-
fore, there is no need to adjust or install the
eyepieces.
Continue with the "Eyecups" section on page
13.

Leica DM750 M Manual 12
Leica Standard Tube with Separate Eyepieces
2. Secure the eyepieces in the tubes by tighte-
ning the silver screws at the bottom.
The eyepieces can still be rotated, but no longer detached from the tubes.
The standard tube includes a rotatable dovetail. Therefore, you can now rotate
the standard viewing tube freely in any orien-
tation.
1. Insert the eyepieces into the tubes.
Standard tubes; tubes do not include eyepieces
yet

Leica DM750 M Manual 13
If you wear eyeglasses for microscope viewing, keep the rubber eyeguards
folded down. If you do not wear eyeglasses, you
may find it useful to unfold the rubber eyegu-
ards in order to help block out ambient room
light.
Eyecups
If you have purchased a standard micro- scope configuration, the objectives are
pre-installed on the objective nosepiece and
the specimen stage condenser is installed
onto the stand. In this case, continue with the
"Use" section starting on page 17. If you have
purchased your Leica DM750 M in individual
components and not according to the standard
configuration, continue with the "Installing
Objectives" section on page 14.

Leica DM750 M Manual 14
Installing Objectives
Installing Objectives
When rotating the objective nosepi- ece, always use the knurled ring on the
objective nosepiece.
While rotating the objective nosepiece clock-
wise, screw the objectives into the objective
nosepiece. Start with the lowest magnification.

Leica DM750 M Manual 15
Installing the polarizing adapter
1. Unscrew the screw and remove the cover.
The opening for the polarizing adapter is
now accessible.
2. Insert the polarizing adapter into the
opening and tighten the screw.
3. Insert the polarizer into the polarizing
adapter.
4. Remove the cover of the analyzer.
5. Push the analyzer into the opening as far as
it will go.
Conversion to Incident Light Polarization

Leica DM750 M Manual 16
Use

Leica DM750 M Manual 17
Switching on the Microscope
Precautionary measures
The light of the Leica DM750 M can be very bright. Therefore, only look into
the eyepieces after you have switched on and
reduced the illumination (see item 3)!
Connecting and switching on the micro-
scope
1. Connect the power supply unit and the
power cable with the incident light axis of
the Leica DM750 M and secure the plug
using the retaining ring on the incident
light axis!
2. Switch on the illumination by briefly pres-
sing the power switch.
3. Look through the eyepieces and adjust the
brightness to the desired level.
The brightness can be adjusted in 15 increments.

Leica DM750 M Manual 18
Aperture diaphragm
The aperture diaphragm of the incident light axis can be opened or closed. An
open aperture diaphragm generates a higher
resolution with a decreased depth of field. A
closed aperture diaphragm, on the other hand,
reduces the quantity of light and resolution
capacity, but the depth of field is increased.
The aperture diaphragm must be completely open for all oblique light illu-
mination scenarios.
Opening / closing the aperture diaphragm
1. Close the aperture diaphragm by moving
the lever upwards.
2. Open the aperture diaphragm by moving
the lever downwards.
Modifying the Aperture Diaphragm

Leica DM750 M Manual 19
Illumination Scenarios
You can adjust the incident light axis illumination to your needs and require-
ments using the membrane control panel.
The aperture diaphragm must be completely open for all oblique light illu-
mination scenarios.
Changing the illumination mode
Press the button in the middle once to switch
between bright-field illumination and oblique
illumination (oblique incident illumination).
Individual light field (oblique light)
Hold a button pressed longer than two seconds
to activate only one of the four light fields for
oblique illumination. Combinations with other
light fields are also possible.
Illumination Scenarios
Bright field illumination
Oblique illumination Individual light field
HOLD

Leica DM750 M Manual 20
Adding light fields
Press a button only briefly to switch an additio-
nal light field on or off.
Switching to bright field
Press the middle button briefly to toggle
between bright-field illumination and the
previously selected oblique light configuration.
Automatically calling up
oblique light illumination scenarios
Hold the middle button pressed longer than
two seconds; as long as you do not release the
button, the next light field is activated every
two seconds.
Illumination Scenarios (continued)
Any arrangement Switching between bright field illumination
and the most recent setting
Switching automatically
between the light fields
HOLD