Leica DM1000 LED User manual

Leica DM1000
Operating manual
Bedienungsanleitung
Mode d’emploi
MICROSYSTEMS

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Published September 2004 by/
Publiée en septembre 2004 par/
Herausgegeben September 2004 von:
Leica Microsystems CMS GmbH
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Verantwortlich für den Inhalt:
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(Clinical Microscopy, Product Management)
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(Safety Officer according to MPG §30/
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3
Leica DM1000
Operating Manual

4
Copyrights
Copyrights
All rights to this documentation are held by
Leica Microsystems CMS GmbH. Reproduction
of text or illustrations (in whole or in part) by
print, photocopy, microfilm or other method (in-
cluding electronic systems) is not allowed with-
out express written permission from Leica
Microsystems CMS GmbH.
The instructions contained in the following
documentation reflect state-of-the-art technol-
ogy and knowledge standards. We have com-
piled the texts and illustrations as accurately as
possible. Nevertheless, no liability of any kind
may be assumed for the accuracy of this manu-
al’s contents. Still, we are always grateful for
comments and suggestions regarding potential
mistakes within this documentation.
The information in this manual is subject to modifi-
cation at any time and without notification.

5
Contents
8.4 Tubes........................................................37
8.5 Eyepieces .................................................... 38
8.6 Objectives ................................................... 39
8.7 Light Sources ............................................. 40
8.8 Aperture Diaphragm ................................. 40
8.9 Field Diaphragm ......................................... 41
9. Contrast Methods ...................................... 42
9.1 Transmitted Light ....................................... 42
9.1.1 Brightfield......................................... 43
9.1.2 Phase Contrast................................ 44
9.1.3 Darkfield ........................................... 44
9.1.4 Oblique Illumination ....................... 45
9.1.5 Polarization ...................................... 45
9.2 Fluorescence.............................................. 46
10. Measurements with the Microscope ... 47
10.1 Linear Measurements .............................. 47
10.2 Thickness Measurements ....................... 48
10.3 Differentiation of Gout / Pseudo Gout ... 49
11. Troubleshooting......................................... 51
12. Care of the Microscope ........................... 54
12.1 Dust Cover .................................................. 54
12.2 Cleaning....................................................... 54
12.3 Handling Acids and Bases ...................... 55
12.4 Changing Fuses.......................................... 55
13. Essential Wear and Spare Parts ............ 56
14. Retrofitting Components .......................... 57
14.1 Equipping the Condenser Disk ................ 57
15. Index ............................................................ 59
16. EU Declaration of Conformity ................. 60
Contents
1. Important Notes about this Manual ...... 6
2. Intended Purpose of the Microscope ... 7
3. Safety Notes ............................................... 8
3.1 General Safety Notes ............................... 8
3.2 Electrical Safety ........................................ 8
4. Overview of the Instrument .................... 10
5. Unpacking the Microscope .................... 14
6. Assembling the Microscope .................. 16
6.1 Stage ............................................................ 16
6.2 Condenser................................................... 18
6.3 Tube and Eyepieces .................................. 19
6.4 Objectives ................................................... 19
6.5 Light Source - Transmitted Light Axis ... 20
6.6 Components for
Fluorescence Applications...................... 21
6.6.1 Fluorescence Illuminator .............. 21
6.6.2 106 z Lamp Housing ........................ 21
6.7 Analyzer and Polarizer* .......................... 24
6.8 Lambda Plate Compensator .................... 24
6.9 Optional Accessories ............................... 25
6.10 Connection to the Power Supply ............ 27
7. Startup ......................................................... 28
7.1 Switching on the Microscope................. 28
7.2 Köhler Illumination .................................... 28
7.3 Checking Phase Contrast Rings ............. 29
7.4 Adjusting the Light Sources .................... 31
8. Operation .................................................... 35
8.1 Switching on............................................... 35
8.2 Stages and Object Displacement ........... 35
8.3 Focusing ...................................................... 36

6
1. Important Notes about this Manual
(1.2)
→ p.20
!
*
Numbers in parentheses, such as "(1.2)", corre-
spond to illustrations (in the example, Figure 1,
Item 2).
Numbers with pointer arrows (for example
→p.20), point to a certain page of this manual.
Caution!
Special safety instructions within this
manual are indicated with the triangle
symbol shown here, and have a gray
background.
Caution! The microscope and accessories can
be damaged when operated incorrectly.
Explanatory note.
Item not contained in all configurations.
Text symbols, pictograms and their meanings:
Caution!
This operating manual is an essential com-
ponent of the microscope, and must be read
carefully before the microscope is
assembled, put into operation or used.
1. Important Notes about this Manual
This operating manual contains important in-
structions and information for the operational
safety and maintenance of the microscope and
accessories. Therefore, it must be kept and
taken care of.

7
2. Intended Purpose of the Microscope
2. Intended Purpose of the Microscope
The Leica DM1000 microscope, to which this
user manual belongs, is designed for biological
routine and research applications. This includes
the examination of samples taken from the hu-
man body with a view to provide information on
physiological or pathological states or
congenital abnormalities, or to determine the
safety and compatibility with potential recipients,
or to monitor therapeutic measures.
The above-named microscope complies with
the Council Directive 98/79/EEC concerning in
vitro diagnostics. It also conforms to the Council
Directives 73/23/EEC concerning electrical
apparatus and 89/336/EEC concerning
electromagnetic compatibility for use in an
industrial environment.
Caution!
The manufacturer assumes no liability for
damage caused by, or any risks arising from
using the microscope for other purposes
than those for which they are intended or
not using them within the specifications of
Leica Microsystems CMS GmbH.
In such cases the conformity declaration
shall cease to be valid.
Caution!
These (IVD) devices are not intended for use
in the patient environment defined by DIN
VDE 0100-710. Neither are they intended for
combining with medical devices according
to EN 60601-1. If a microscope is electrically
connected to a medical device according to
EN 60601-1, the requirements defined in
EN 60601-1-1 shall apply.

8
3. Safety Notes
3. Safety Notes
3.2 Electrical Safety
General Specifications
Microscope
For indoor use only.
Supply voltage:
Frequency:
Power input:
Fuses:
Ambient temperature:
Relative humidity:
Over voltage category:
Pollution degree:
Caution!
The power plug may only be plugged into an
outlet equipped with a grounding contact.
Do not interfere with the grounding function
by using an extension cord without a ground
wire. Any interruption of the ground wire in-
side or outside of the device, or release of
the ground wire connection, can cause the
device to become hazardous. Intentional
ground interruption is not permitted!
90-250 V~
50-60 Hz
90 W
F 3,15 A 250 V
15-35°C
max. 80% to 30°C
II
2
3.1 General Safety Notes
This safety class 1 device is constructed and
tested in accordance with
EN 61010-2-101:2002,
EN 61010-1:2001,
IEC 1010-1:2001,
safety regulations for electrical measuring, con-
trol, and laboratory devices.
Caution!
In order to maintain this condition and to en-
sure safe operation, the user must follow the
instructions and warnings contained in this
operating manual.
Caution!
The devices and accessories described in
this operating manual have been tested for
safety and potential hazards.
The responsible Leica affiliate or the main
plant in Wetzlar, Germany must be consulted
whenever the device is altered, modified or
used in conjunction with non-Leica
components that are outside of the scope of
this manual.
Unauthorized alterations to the device or
noncompliant use shall void all rights to any
warranty claims!

9
3. Safety Notes
Caution!
Protect the microscope from excessive tem-
perature fluctuations. Such fluctuations can
lead to the accumulation of condensation,
which can damage the electrical and optical
components.
Ambient temperature: 15-35°C.
Caution!
Before exchanging the fuses or lamps, be
absolutely certain to switch off the main
power switch and remove the power cable.
Caution!
Never use any fuses as replacements other
than those of the types and the current rat-
ings listed here. Using patched fuses or
bridging the fuse holder is not permitted.
The use of incorrect fuses may result in a
fire hazard.
Caution!
The microscope’s electrical accessory com-
ponents are not protected against water.
Water can cause electric shock.

10
4. Overview of the Instrument
4. Overview of the Instrument
Specification
Contrast Method
Transmitted Light Axis
Incident Light Axis
Tube
Magnification Changer
(optional)
Objective Turret
X/Y Stage
Leica DM1000
• Transmitted Light: Brightfield, Darkfield,
Phase Contrast, Polarization
• Incident Light: Fluorescence
Integrated halogen illumination
manual adjustment of
• Light Intensity
• Aperture Diaphragm
• Field Diaphragm (only with Koehler kit)
Incident light fluorescence illuminator for up to eyepiece
field number 20 with
• Interchangeable slide with mount for 3 filter systems
• Adjusting lens for lamp
• Light trap for the suppression of extraneous light
• BG38 blue filter and shutter, switchable
optionally with
• Fixed or variable viewing angle
• Up to 3 switching positions
• one or two camera ports
• Ergotube with height-adjustable eye level and camera port
• Manual
• Magnification steps: 1x; 1.5x; 2x
• Manual
• 5-fold for objectives with M25 thread
• With condenser holder
• Coaxial pinion optional telescopable
• Controls mountable on left or right

11
4. Overview of the Instrument
Specification
Condenser
Focusing
Leica DM1000
• CL/PH 0.90/1.25 OIL condenser with color coding
• CLP/PH 0.85 condenser for polarization
• Achr.apl. A 0.9 (P) condenser with swivelable condenser head
• UCL 0.90/1.25 OIL universal condenser UCLP 0.85 for
polarization with 5-position light ring disk)
• UCL/P pol. universal condenser with interchangeable
condenser head and condenser disk with 6 positions
• Focus wheel for coarse and fine focusing
• Height adjustment

12
4. Overview of the Instrument
Fig. 1 Left side of the Leica DM1000 stand
1Coarse and fine focusing
2Condenser height adjustment
3Brightness control
4Field diaphragm
5Aperture diaphragm
6Condenser
6
5
4
12 3

13
4. Overview of the Instrument
Fig. 2 Right side of the Leica DM1000 stand
1Eyepiece
2Eyepiece tube
3Tube
4Objective turret with objectives
5Specimen stage with specimen holder
6 Integrated illumination
7On/Off switch
8Condenser height adjustment
9Coarse and fine focusing
10 Coaxial pinion for x/y stage movement
1
2
3
4
5
678910

14
5. Unpacking the Microscope
Installation Location
Work with the microscope should be performed
in a dust-free room, which is free of oil vapors
and other chemical vapors, as well as extreme
humidity. At the workplace, large temperature
fluctuations, direct sunlight and vibrations
should be avoided. These conditions can distort
measurements and micrographic images.
Allowable ambient conditions
Temperature 15-35°C
Relative humidity maximum 80% up to 30°C
Microscopes in warm and warm-damp climatic
zones require special care in order to prevent
the build up of fungus.
See the chapter "Care of the Microscope" →
p. 54,
for additional instructions.
Caution!
Electrical components must be placed at least
10 cm away from the wall and away from
flammable substances.
First, carefully remove all components from the
transportation and packaging materials.
Note:
If at all possible, avoid touching the lens sur-
faces of the objectives. If fingerprints do appear
on the glass surfaces, remove them with a soft
leather or linen cloth. Even small traces of finger
perspiration can damage the surfaces in a short
time. See the chapter "Care of the Microscope"
→
p. 54, for additional instructions.
Caution!
Do not yet connect the microscope and pe-
ripherals to the power supply at this point!
5. Unpacking the Microscope

15
5. Unpacking the Microscope
Transport
For shipping or transporting the microscope
and its accessory components, the original
packaging should be used.
As a precaution to prevent damage from vibra-
tions, the following components should be dis-
assembled and packaged separately:
• Unscrew the objectives.
• Remove the condenser.
• Remove the coaxial pinion.
• Remove the lamp housings.
• Disassemble the burner of 106 z lamp housing.
• Remove all moving or loose parts.

16
6. Assembly
!
Fig. 3 Specimen stage with specimen holder
1Lock screws for specimen holder
The microscope components are logically as-
sembled in this order:
• Stage
• Condenser
• Fluorescence*
• Intermediate systems*
• Tube
• Eyepieces
• Objectives
• Lamp housings with light sources
• Polarization*
Only one commonly used screwdriver is
necessary for assembly, which is included in the
delivery package.
The tool can be stored on a magnetic retainer on
the underside of the stage at the right.
When using intermediate systems and optical
accessories, the sequence may vary.
In this case, read Chapter
"6.9 Optional Accessories" →p. 25.
6.1 Stage
Caution:
Before completing the stage, make sure no ob-
jectives are installed!
Remove the srew located under the stage in the
front.
Specimen Holder
• Place the specimen holder on the stage and
fasten it with the two screws (3.1).
Coaxial Pinion
Note:
The coaxial pinion can be mounted on the left-
or right-hand side.
1
6. Assembling the Microscope

17
6. Assembly
•First, place the fine focus wheel on the side to
which you intend to mount the coaxial pinion.
The wheel is held in place magnetically (4.1).
Ensure that the button snaps into place.
Attach the other focus knob on the opposite
side.
•Loosen the lock screw (5.1) at the front left-
hand side of the stage.
•Slide the stage as far back as possible.
•Attach the coaxial pinion with the srew (6.1).
•Return the stage to the starting position and
retighten the lock screw. After installation of
the stage control, move object guide all the
way to the left side of the instrument. Keep
turning when guide has reached the end of
travel until a click noise is heard.
Adjusting the focus stop
The focus stop is preadjusted by the factory to
prevent collision with objectives. The focus stop
should be set ca.0,3 mm higher as the focal pla-
ne to enable focussing of the samples of diffe-
rent thickness.
Fig. 6 Coaxial pinion installation
1Mounting screw for coaxial pinion
1
1
Fig. 4 Focus wheel
1Magnetic retainer for fine focus wheel
Fig. 5 Underside of stage
1Lock screw
1
If a readjustment is necessary, adjust as
following:
•Lower the stage by rotating 1/2 turn of the
coarse adjustment knob
•Loosen the focus stop screw on left hand side
of microscope
•Move stage to desired focal plane (preferably
ca. 0.3 mm higher)
•Tighten focus stop screw

18
6. Assembly
6.2 Condenser
• If present, screw the condenser head into the
condenser.
• Using the condenser height adjuster (9.3), turn
the condenser holder (fig. 8) completely
downward.
• Unscrew the clamping screw for the con-
denser (9.2) far enough so that the condenser
can be inserted from the front.
• From the front, insert the condenser into the
condenser holder as far as it will go. On the
underside of the condenser, there is an orien-
tation pin (7.1), which must be located in the
guiding notch (8.1).
• Pull the condenser’s clamping screw (9.2) so
that the condenser is locked in place.
Fig. 9 Condenser holder
1Condenser centering
2Clamping screw for condenser
3Condenser height adjuster
Fig. 8 Condenser holder
1Guiding notch
1
Note:
The condenser must be centered before using
the microscope.
→
Köhler illumination p. 28.
Fig. 7 Underside of condenser(example CL/PH)
1Orientation pin
2Auxiliary condenser lens LS
2
11
3
2
1
3
1

19
6. Assembly
6.3 Tube and Eyepieces
Note:
For fluorescence applications, install the
fluorescence illuminator first →p. 21.
If available, the analyzer* (10.1) must be inserted
into the stand. This requires that the guide key
engages in the guide pin (10.2).
To mount the analyzer, the analyzer mount TL*
20 mm or 60 mm can also be placed between
stand and tube.
An intermediate tube pole* with a switchable
analyzer (on/off) and Bertrand lens is also
available as an option.
The tube is mounted to the stand either directly or
with the use of intermediate modules.
Fig. 11 Fastening the tube
1Clamping screw
1
!
Fig. 10 Analyzer mounting
1Analyzer
2Orientation pin and guiding notch
3Clamping screw
• Loosen the clamping screw (11.1) on the
stand.
• Insert the tube in the circular receptacle
(dovetail ring).
• Retighten the clamping screw (11.1).
• The eyepieces are inserted into the eyepiece
tubes on the tube.
6.4 Objectives
Always only use Leica objectives of tube length
× (infinity)! The standard thread is M25. The
objectives should be arranged so that the
magnification increases when the objective
nosepiece is rotated counter clockwise.
Attention:
Lower the specimen stage as far as possible
before assembling the objectives. Close vacant
threads in the nosepiece with dust protection
caps!

20
6. Assembly
6.5 Light Source for the Transmitted Light Axis
Caution!
Be sure that the lamp housing is discon-
nected from the power supply. Unplug the
power plug and the power supply during as-
sembly.
Caution!
Light sources pose a potential irradiation
risk (glare, UV-radiation, IR-radiation).
Therefore, lamps have to be operated in
closed housings.
Replacing the Lamp of the Integrated
Illumination
The transmitted light illumination with a low-
voltage tungsten halogen lamp (Fig. 12) is
integrated in the base of the microscope and is
accessible from the right-hand side.
• Remove the insert (12.2).
Caution!
The lamp may still be hot!
• Remove the lamp.
Caution!
Do not remove the new lamp’s dust cover
until you have installed the lamp. Avoid
fingerprints on the lamp.
• Insert the new lamp with the dust cover
straight into the socket until it stops. Be sure
that the lamp is inserted straight.
• Remove the lamp’s dust cover.
• Replace the insert (12.2).
Fig. 12 Transmitted-light illumination in microscope base
1Tungsten halogen lamp
2Insert
12