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1) To"acquire"an"image,"select"the"Acquire,tab"in"the"middle"portion"of"the"screen"and"the"Acquire,
tab"selected"in"the"left"portion"of"the"screen."
2) To"perform"a"sequential"scan,"hit"the"seq"button.""
3) Format-"refers"to"how"many"pixels"will"be"captured"when"you"acquire"an"image."Use"a"smaller"
pixel"format"when"trying"to"find"an"area"of"interest"and"a"larger"pixel"format"when"taking"a"
picture."
4) Speed-"refers"to"how"quickly"the"laser"passes"over"each"pixel."Slower"speeds"can"cause"photo"
bleaching,"but"will"give"better"signal-to-noise"ratio."""
5) Line-,average"reduces"the"background."A"higher"average"will"increase"the"time"to"acquire"an"
image,"but"it"should"increase"the"quality"of"your"image.""
6) Frame,averaging-,is"similar"to"line"averaging,"but"it"averages"noise"in"each"frame"instead"of"per"
line."
7) Pinhole-,the"size"of"the"opening"which"the"laser(s)"pass"through."Leave"at"Airy"1,"unless"there"is"
'&(&<C"#(E3["to"increase"the"signal."
8) Z-stack-,select"the"begin"and"end"limits"of"your"z-stack"(more"on"this"later)."
9) Start"live"scan"to"acquire"image."
10) Once"all"the"settings"are"correct"hit"the"capture"image"button."All"images"are"can"be"found"in"the"
“Projects”"tab."
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