Leica ES2 User manual

Leica ES2
Leica EZ4
Leica EZ4 W
User Manual

Leica E Series User Manual 2
Congratulations!
You have just purchased a Leica E Series stereo-
microscope – an excellent choice! Viewing
entire objects under magnification imparts
perspectives and discoveries that would not be
possible with the naked eye.
In developing our stereomicroscopes, we have
placed great emphasis on simple, self-explan-
atory operation. However, please take the time
to read the instruction manual and the notes on
operating safety, to learn about all the features
and capabilities of your instrument so that
you can use it safely and to its best advantage.
Should you have any questions, please consult
your local Leica representative. We are gladly at
your service!

Leica E Series User Manual 3
Chapter Overview
Safety and Use 6
Leica ES2 14
Leica EZ4 and Leica EZ4 W 22
Eyepieces (only for Leica EZ4) 33
Photography Using the Leica EZ4 W 41
Get Set! 47
The Camera Remote Control (Optional) 55
Care, Transport, Contact Persons 68
Specifications 70
Dimensions 72

Leica E Series User Manual 4
Safety and Use
Symbols Used 7
Safety Instructions 8
Regulatory Compliance Information 10
Instructions for Use 12
Leica ES2
Overview: Leica ES2 15
Transport, Placement and Storage 16
The Correct Interpupillary Distance 17
Using the LED Illuminator 18
Focusing 19
Magnification Display 20
Adjusting the Resistance of the Focus Drive 21
Leica EZ4 and Leica EZ4 W
Overview: Leica EZ4 23
Instrument Variant: Leica EZ4 W 24
Transport, Placement and Storage 25
The Correct Interpupillary Distance 26
Switching on the LED Illuminator 27
Controlling the Automatic Shutoff 28
Illumination Types 29
Focusing 30
Contents
Magnification Display 31
Adjusting the Resistance of the Focus Drive 32
Eyepieces (Only for Leica EZ4)
Changing Eyepieces (Only for Leica EZ4, Open Version) 34
Eyepieces and Eyecups (Only for Leica EZ4, Open Version) 35
Dioptric Correction (Only for Leica EZ4, Open Version) 36
Graticules (Only for Leica EZ4, Open Version) 38
Graticules: Preparation 39
Inserting the Graticules 40
Photography using the Leica EZ4 W
Introduction 42
The Basics 43
Leica EZ4 W: Overview 44
Unpacking 46
Get Set!
USB Mode – Capturing and Adjusting Images Using a Computer 48
SD Mode – Capturing Images Without a Computer or
Without a Wireless Device 50
Adjusting Settings While in SD Mode 52
WiFi Mode – Capturing and Adjusting Images Using a Wireless Device 53
Ethernet Mode – Capturing and Adjusting Images Using a Network 54

Leica E Series User Manual 5
Contents (Continued)
The Camera Remote Control (Optional)
Optional Remote Control 56
Viewing Images and Movies With the Optional Remote Control 57
Calling up the Camera Menu 58
COLOR (Automatic White Balance) 59
COLOR (Manual White Balance) 60
EXPOSURE 61
RESOLUTION 62
SETUP CAMERA (Camera Settings) 63
SETUP ETHERNET 64
SETUP WiFi 65
Pairing Cameras With Remotes 67
Care, Transport, Contact Persons
Care, Transport, Contact Persons 69
Specifications
Specifications 71
Dimensions
Leica ES2 73
Leica EZ4 W 75
Leica EZ4, 10× Eyepieces 77
Leica EZ4 without Eyepieces 79

Leica E Series User Manual Safety and Use 6
Safety and Use

Leica E Series User Manual Safety and Use 7
Symbols Used
Read and understand user manual before using this device.
Warning of a danger
•This symbol indicates especially impor- tant information that must be read and
complied with. Failure to comply can cause the
following:
OHazards to personnel
OFunctional disturbances or damaged
instruments
Warning of hazardous electrical voltage
This symbol indicates especially impor- tant information that, if not observed,
can cause the following:
OHazards to personnel
OFunctional disturbances or damaged
instruments
Danger due to hot surface
This symbol warns against touching hot surfaces, e.g. those of light bulbs.
Important information
This symbol indicates additional infor- mation or explanations that intend to
provide clarity.

Leica E Series User Manual Safety and Use 8
Before installing, operating or using the instrument, it is mandatory to read this
user manual. In particular, please observe all
safety instructions.
The "Safety Concept" brochure contains addi-
tional safety information regarding service
work, requirements and the handling of the
microscope, accessories as well as general
safety instructions.
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Warning of a danger
This symbol indicates especially impor- tant information that is mandatory
to read and observe. Failure to follow these
instructions may pose hazards to personnel,
cause instrument malfunctions or damage the
instrument.
Hazardous electrical voltage
This symbol indicates especially impor- tant information that is mandatory
to read and observe. Failure to follow these
instructions may pose hazards to personnel,
cause instrument malfunctions or damage the
instrument.
Important information
This symbol indicates additional infor- mation or explanations that intend to
provide clarity.
EC Declaration of Conformity
Electrically operated accessories are
constructed based on the state of the art of
technology and are provided with an EC Decla-
ration of Conformity.
Technical Safety
To connect the Leica E Series microscopes to
power, use a power cable that meets the power
cable specifications in your country. The power
socket used has to be equipped with a 16 A or
10 A fuse. Only connect the instrument to a
grounded power socket that has been installed
according to regulations. The power supply
voltage has to correspond to the voltage speci-
fied on the identification label. Make sure that
the ground connection is neither defective
nor interrupted, since otherwise a malfunction
poses a risk of fatal injury. Never unplug the
power plug with moist or wet hands. There is
danger of an electric shock.
Safety Instructions

Leica E Series User Manual Safety and Use 9
Safety Instructions (Continued)
Instrument safety and EMC
Our device has been designed, produced and
tested in compliance with
OIEC EN 61010-1: Electrical equipment for
measurement, control and laboratory use
– Safety – Part one: general requirements
OEN 60950-1: Information technology equip-
ment – Safety – Part one: general require-
ments
ORadio interference suppression in compli-
ance with EN 55011 class B
ONoise immunity in compliance with
DIN EN 61326-1.
The device meets the requirements of EU
directives
O2006/95/EG Low voltage directive
O2014/30/EU EMC directive
O2011/65/EU RoHS directive
and carries the CE mark.
The device must be disposed of in accordance with 2012/19/EU WEEE
directive.
Intended for indoor use only in all EU member
states, EFTA states and Switzerland.
Leica EZ4 W
OEN 300328: Electromagnetic compatibility
and Radio spectrum Matters (ERM); Wide-
band transmission systems; Data transmis-
sion equipment operating in the 2,4 GHz
ISM band and using wide band modulation
techniques; Harmonized EN covering essen-
tial requirements under article 3.2 of the
R&TTE directive.

Leica E Series User Manual Safety and Use 10
Regulatory Compliance Information
FCC Requirements for Operation in the United States
FCC radio frequency interference warnings
and instructions
This equipment has been tested and found to
comply with the limits for a Class B digital
device, pursuant to Part 15 of the FCC Rules.
These limits are designed to provide reason-
able protection against harmful interference
in a residential installation. This equipment
generates, uses and can radiate radio frequency
energy and, if not installed and used in accor-
dance with the instructions, may cause harm-
ful interference to radio communications.
However, there is no guarantee that interfer-
ence will not occur in a particular installation.
If this equipment does cause harmful interfer-
ence to radio or television reception, which can
be determined by turning the equipment off
and on, the user is encouraged to try to correct
the interference by one or more of the follow-
ing measures:
OReorient or relocate the receiving antenna.
OIncrease the separation between the equip-
ment and receiver.
OConnect the equipment into an outlet on
a circuit different from that to which the
receiver is connected.
OConsult the dealer or an experienced radio/
TV technician for help.
FCC information to user
This product does not contain any user service-
able components and is to be used with
approved antennas only.
Any product changes or modifications will
invalidate all applicable regulatory certifica-
tions and approvals.
FCC guidelines for human exposure
This equipment complies with FCC radiation
exposure limits set forth for an uncontrolled
environment. This equipment should be
installed and operated with minimum distance
of 20 cm between the radiator and your body.
This transmitter must not be co-located or oper-
ating in conjunction with any other antenna or
transmitter.
FCC caution
OAny changes or modifications not expressly
approved by the party responsible for
compliance could void the user's authority
to operate this equipment.
OThis device complies with Part 15 of the
FCC Rules. Operation is subject to the
following two conditions: (1) This device
may not cause harmful interference, and
(2) this device must accept any interference
received, including interference that may
cause undesired operation.
OThis device and its antenna(s) must not be
co-located or operation in conjunction with
any other antenna or transmitter.

Leica E Series User Manual Safety and Use 11
Canada (IC)
This device complies with Industry Canada
licence-exempt RSS standard(s). Operation is
subject to the following two conditions:
(1) this device may not cause interference, and
(2) this device must accept any interference,
including interference that may cause unde-
sired operation of the device.
Canada (IC)
Le présent appareil est conforme aux CNR
d'Industrie Canada applicables aux appareils
radio exempts de licence. L'exploitation est
autorisée aux deux conditions suivantes:
(1) l'appareil ne doit pas produire de brouillage,
et
(2) l'utilisateur de l'appareil doit accepter tout
brouillage radioélectrique subi, même si le
brouillage est susceptible d'en compromettre
le fonctionnement.
Australia and New Zealand Notices
This device equipment complies with the
Australian and New Zealand regulatory approv-
als requirements.
Regulatory Compliance Information (Continued)

Leica E Series User Manual Safety and Use 12
Intended use
The E Series microscopes are optical instru-
ments for improving the visibility of objects
through magnification and illumination. It is
used for observing and documenting.
Place of use
Use the instrument in enclosed, dust-free
rooms at +10°C to +40 °C only. Protect it from
oil, chemicals and extreme humidity. It must
be at least 10 cm from the wall and away from
flammable substances.
Interference
Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
images.
In warm and warm-damp climatic zones, the individual components require
special care in order to prevent the build-up of
fungus.
Use in clean rooms
Leica E Series microscopes can be used in clean
rooms without any problems.
Handling electrical components
Never install any other plug (NEMA 5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
The microscope must be connected to a grounded outlet.
Always position the microscope so that you can disconnect it from the power
supply at any time. The power cable is provided
as the power disconnect device.
Instructions for Use

Leica E Series User Manual Safety and Use 13
Intervention from the user
The microscopes of the E Series contain no components that can be main-
tained or repaired by the user. Exceptions are
mentioned specifically in this manual.
Unless otherwise specified in this manual, repair and service work may
be carried out by authorized Leica technicians
only.
Unauthorized alterations to the instru- ment or noncompliant use shall void all
rights to any warranty claims.
Maintenance
Leica E Series microscopes are basically maintenance-free. To ensure that it
always operates safely and reliably, we recom-
mend that you take the precaution of contact-
ing the responsible service organization.
Here you can arrange for periodic inspections
and conclude a maintenance contract (recom-
mended).
Accessories
Only those accessories may be used that are
listed in this user manual or for which safe use
has been confirmed by Leica Microsystems.
Danger of infection
Direct contact with eyepieces is a poten- tial transmission method for bacterial
and viral infections of the eye. The risk can be
kept to a minimum by using personal eyepieces
for each individual or detachable eyecups.
Dangers during use
The microscope illumination is in the exempt
group (risk group 0) according to EN 62471:2008
when used according to its intended use.
•Never look directly into the LED beam of the illumination equipment – either
with or without optical instruments – as this
increases the risk class. Failure to observe this
notice poses a risk of eye damage.
Instructions for Use (Continued)

Leica E Series User Manual Leica ES2 14
Leica ES2

Leica E Series User Manual Leica ES2 15
Overview: Leica ES2
1
2
3
5
7
6
4
1. 10×fixed eyepieces for eyeglass wearers
2. Carrying handle
3. Two-level magnification changer 10×and 30×
4. Focusing drive
5. Integrated LED incident light illumination
6. Integrated LED transmitted illumination
7. Switch for incident and transmitted light

Leica E Series User Manual Leica ES2 16
Built-in carrying device
All Leica E Series microscopes are equipped with a carrying handle for
convenient and safe transport.
Setting up the microscope
1. Place the microscope on a flat tabletop.
2. Connect the power supply to a grounded
socket.
Transport, Placement and Storage
Packing the device away
1. After use, wind up the cable as shown
in the illustration.
2. Store the instrument so that it is protected
against dust.

Leica E Series User Manual Leica ES2 17
Setting the eye distance
1. Look into the eyepieces.
2. Hold the eyepieces with both hands. Push
the eyepieces together or separate them
until you see a circular image.
Note for eyeglass wearers
Ifyouwearglasses,foldtheeyecupsback– otherwise, fold the eyecups forwards.
The Correct Interpupillary Distance
The ideal image
The interpupillary distance is correctly set if you see a single circular image field
when looking at a specimen.
You may need a short time to become familiar
with using the instrument. Not to worry – after
a little while, it will become automatic.



Leica E Series User Manual Leica ES2 18
Iluminating the specimen
1. Position a specimen in the middle of the
baseplate.
2. Switch the two LED illuminators on or off as
desired.
After switching off, the LEDs remain lit for about another 4 seconds.
Tips on use
OUse incident light for plastic, opaque
objects.
OUse transmitted light for transmitted light
specimens or transparent objects.
OUse the combination of both LED illumina-
tors for partially transparent objects.
Using the LED Illuminator

Leica E Series User Manual Leica ES2 19
Focusing raises and lowers the stereo- microscope using the focusing drive. The
specimen is brought into sharp focus as soon as
it is in the focal point of the objective.
The focusing drive can be operated either left-
or right-handed.
Focusing
1. Set the magnification changer to the lowest
level (10).
2. Set the working distance to approx.
100mm for coarse focusing.
3. Set the magnification changer to the
second level (30).
4. Use the focusing drive once again for fine
focus.
After you have adjusted the image sharpness at high magnification, it
is maintained even if you switch to a lower
magnification (parfocal).
Focusing
Focusing drive torque

Leica E Series User Manual Leica ES2 20
Magnification Display
You can read the configured magnifica- tion on the right-hand rotary knob of
the Leica ES2. The additional magnification
provided by the eyepieces is already incorpo-
rated in the scale.