logos biosystems LUNA FX7 Series User manual

User Manual
www.logosbio.com
LBSM-SM-ML-LU7-001
Rev. 6

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DISCLAIMER
The contents of this document are subject to change without notice.
The LUNA-FX7™ Automated Cell Counter is an electrical laboratory instrument for scientific research use only.
It is not a medical, therapeutic, or in vitro diagnostics device.
Do not disassemble the device on any occasion as this will invalidate your warranty.
TRADEMARKS
The trademarks used in this document are the property of Aligned Genetics, Inc.
©2021 Aligned Genetics, Inc. All rights reserved.
Logos Biosystems is the brand name of Life Science business of Aligned Genetics, Inc.

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CERTIFICATION MARKS
The WEEE (Waste Electrical and Electronic Equipment) symbol indicates that users of this
instrument have responsibility of returning and disposing of WEEE in an environmentally friendly
manner.
Follow the waste ordinances of your region for proper disposal provisions.
The CE mark indicates that this instrument conforms to all applicable European Community
provisions for which this marking is required. Users must be aware of and follow the conditions
described in this manual for operating the instrument. The protection provided by the instrument
may be impaired if the instrument is used in a manner not specified by this manual.
Protective earth (Ground)
This device complies with Part 15 of the FCC Rules.
This equipment complies with the requirement of UL 61010-1:2012, CAN/CSA C22.2 No.61010-
1:2012.
“Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part
1: General Requirements.”
The KC certification mark indicates that this instrument conforms with Korea’s product safety
requirements for electrical and electronic equipment and components for which this marking is
required.

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Table of Contents
SAFETY PRECAUTIONS ·········································································································· 7
Instrument Safety ·················································································································· 7
General Safety ···················································································································· 7
Operating Conditions ············································································································ 7
Instrument Disposal ············································································································· 7
Instrument Disassembly ········································································································ 7
Personal Safety ····················································································································· 7
Safety Guidelines ················································································································ 7
Waste Disposal ··················································································································· 8
PRECAUTIONS DE SECURITE ··································································································· 9
Sécurité des instruments ······································································································ 9
Sécurité générale ··············································································································· 9
Conditions de fonctionnement ···························································································· 9
Destruction de l’instument ·································································································· 9
Démontage de l’instrument ································································································· 9
Sécurité personnelle ············································································································ 9
Consignes de sécurité ······································································································ 10
Traitement des déchets ···································································································· 10
1. PRODUCT INTRODUCTION ····························································································· 11
Product Contents ·················································································································· 11
Product Contents ··············································································································· 11
Product Description ··············································································································· 12
LUNA-FX7™ Automated Cell Counter ···················································································· 12
LUNA-FX7™ Cell Counting Slides ························································································ 12
2. GETTING STARTED ··········································································································· 14
Installation ·························································································································· 14
Installation ······················································································································· 14
LUNA-FX7™ Startup ·········································································································· 14
Screen Saver ···················································································································· 15
3. COUNTING CELLS ············································································································· 16

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Sample Preparation ·············································································································· 16
Sample Staining ················································································································ 16
Sample Loading ················································································································ 16
Counting with the LUNA-FX7™ ······························································································· 16
Slide Insertion & Removal ··································································································· 16
Viewing Images ················································································································· 17
Cell Counting ···················································································································· 18
Results ······························································································································· 19
Results ···························································································································· 19
Histograms ······················································································································· 20
Dilution Calculator ············································································································· 20
Save ······························································································································· 21
Print ································································································································ 22
Quick Save ······················································································································ 22
4. REVIEW ································································································································ 23
Reviewing Data ···················································································································· 23
Review Images ················································································································· 23
Reanalyze ························································································································ 24
Previous Counts ················································································································ 24
5. PROTOCOLS ······················································································································· 25
Protocol Selection ················································································································· 25
Default Protocol ················································································································ 25
Creating Protocols ············································································································· 25
Editing Protocols ·················································································································· 26
Load Protocol ··················································································································· 26
Protocol Parameters ············································································································· 27
Brightfield Cell Counting Parameters ····················································································· 27
Min./Max. search size ········································································································· 28
Cell detection sensitivity ······································································································ 28
Live cell sensitivity ············································································································· 28
Noise reduction ················································································································· 28
Dilution factor ··················································································································· 28
Fluorescence Cell Counting Parameters – Cell lines & Primary cells mode ···································· 28
GF/RF Exposure Level ······································································································· 29
Cell Size Calculation ·········································································································· 29
Min./Max. cell size ············································································································· 29

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GF/RF threshold level ········································································································· 29
Dilution Factor ·················································································································· 29
Fluorescence Cell Counting Parameters – Cell lines & Primary cells, Advanced mode ····················· 30
GF/RF Exposure Level ······································································································· 30
Min./Max. search Size ········································································································ 30
Declumping sensitivity ········································································································ 30
Min. FL intensity ················································································································ 31
Min. roundness ················································································································· 31
Dilution Factor ·················································································································· 31
6. BIOPROCESS FEATURE ·································································································· 32
Bioprocess Feature ··············································································································· 32
Bioprocess ······················································································································· 32
Creating a Bioprocess Protocol ···························································································· 32
Record Bioprocess Data ····································································································· 32
Review/Export Bioprocess Data ···························································································· 33
Graph ····························································································································· 33
7. QUALITY CONTROL ·········································································································· 34
Quality Control Mode ············································································································· 34
Quality Control ·················································································································· 34
Validation Slide Registration ································································································ 34
Performing Quality Control ·································································································· 37
Review ···························································································································· 37
8. SETTINGS ···························································································································· 39
LUNA-FX7™ Settings ············································································································ 39
Screen Settings ················································································································· 39
Cell Counting ···················································································································· 39
Save & Review ················································································································· 42
Network ··························································································································· 42
CountWire ························································································································ 44
Date & Power ··················································································································· 45
S/W & Calibration ·············································································································· 46
9. DATA TRANSFER VIA NETWORK ··················································································· 48
Network sharing ··················································································································· 48

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Connected to User PC ········································································································ 48
10. MAINTENANCE AND TROUBLESHOOTING ······························································· 51
Maintenance ························································································································ 51
Powering on/off ················································································································· 51
Cleaning ·························································································································· 51
Troubleshooting ··················································································································· 51
Inaccurate Cell Count ········································································································· 51
Slide Insertion ··················································································································· 52
Data Transfer and Saving ···································································································· 52
Software Update Errors ······································································································ 52
11. PRODUCT SPECIFICATIONS ························································································· 53
LUNA-FX7™ Automated Cell Counter ······················································································· 53
Physical and Technical Characteristics ··················································································· 53
LUNA™ Slides ····················································································································· 53
Physical Characteristics ······································································································ 53
12. ORDERING INFORMATION ···························································································· 54
Instruments ························································································································· 54
Slides and Reagents ············································································································· 54
CountWire™ ························································································································ 55
IQ/OQ ································································································································ 55
Accessories ························································································································· 55
13. PURCHASER NOTIFICATION ························································································ 56
Limited Use Label License ······································································································ 56
Research Use Only ············································································································ 56
Instrument Warranty ·············································································································· 56
Warranty ·························································································································· 56
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7
Safety Precautions
Instrument Safety
General Safety
Operate the instrument in the conditions described in the Operating Conditions.
Install the instrument on a level and sturdy surface. Avoid vibrations from other devices.
Do not touch components with wet hands.
Use components provided or authorized by Logos Biosystems. If the proper combination of
components is not used, product safety cannot be guaranteed.
Use only the power cord and AC adapter provided by Logos Biosystems. If the proper power
cord and AC adapter are not used, electrical safety of the product cannot be guaranteed.
Ensure that the input voltage is compatible with the power supply voltage of the product.
Connect the grounding terminal of the instrument and electrical outlet properly. If the
instrument is not grounded, electrical safety of the product cannot be guaranteed.
Turn on the instrument only after connecting the power cord and AC adapter to both the power
source and the instrument. Turn off the instrument before disconnecting the power cord and/or
moving the instrument.
Disconnect the power cord in the case of abnormalities.
Be careful with possible electric shock hazards as electric current may be still alive when the
instrument stops.
Do not hold the slide slot while it is in motion.
Protect USB drives from being infected with viruses and malware.
Before shutting down or moving the device, remove the slides from the slide holder. If slides
are left inside the slide holder, they may fall into the internal parts of the equipment and cause
malfunctions.
Operating
Conditions
Operating Power
100 - 240 VAC, 1.5 A
Frequency
50/60 Hz
Electrical Input
12 VDC, 5.0 A
Installation Site
Indoor use only
Operating Temperature
10 - 35°C
Maximum Relative Humidity
10 - 80%
Altitude
≤ 2,000 m
Instrument
Disposal
Follow the rules and regulations of your local government.
Instrument
Disassembly
Do not disassemble the instrument in any event as this will invalidate your warranty.
If the instrument is damaged or malfunctioning, contact your local distributor or Logos
Biosystems.
Personal Safety
Safety Guidelines
Read and understand all user manuals thoroughly before using the instrument.

8
Keep all user manuals in a safe and accessible place for future reference.
Read and understand all safety data sheets before storing, handling, or working with any
reagents.
Wear appropriate personal protective equipment (PPE) when handling reagents and cell
samples to avoid exposure.
When using toxic agents, radioactive materials, or pathogenic microorganisms belonging to
WHO Risk Groups 2-4, follow national laws and regulations for biosafety level requirements.
This instrument is to be serviced by trained personnel only to avoid injury.
Waste Disposal
Do not reuse disposable slides. Used slides must be disposed as biohazardous waste
according to the rules and regulations of your local government.

9
Précautions de sécurité
Sécurité des instruments
Sécurité générale
Faites fonctionner l'instrument dans les conditions décrites dans les conditions de
fonctionnement.
Installez l'instrument sur une surface plane et solide. Évitez les vibrations provenant des
autres appareils.
Ne touchez pas les composants avec les mains mouillées.
Utilisez uniquement les composants fournis ou autorisés par Logos Biosystems. En cas
d’utilisation d’une combinaison autre que celle qui a été recommandée, la sécurité du produit
ne peut être garantie.
Utilisez uniquement le cordon d’alimentation et l’adaptateur fournis par Logos Biosystems. En
cas d’utilisation du cordon et de l’adaptateur non appropriés, la sécurité electrique du produit
ne peut être garantie.
Assurez que la tension d’entrée est compatible avec la tension d’alimentation du produit.
Connectez correctement la borne de mise à la terre de l'instrument et la prise électrique. Si
l'instrument n'est pas mis à la terre, la sécurité électrique du produit ne peut pas être garantie.
Allumez l'instrument uniquement après avoir connecté respectivement le cordon
d'alimentation et l'adaptateur à la source d'alimentation et à l'instrument. Éteignez l'instrument
avant de débrancher le cordon d'alimentation et / ou de déplacer l'instrument.
Débranchez le cordon d’alimentation en cas d’anomalies.
Soyez prudent avec les risques d'électrocution, car le courant électrique peut être encore actif
lorsque l'instrument s'arrête.
Ne tenez pas le tiroir de lame lorsqu'elle est en mouvement.
Protégez les clés USB contre les virus et les logiciels malveillants.
Avant d'éteindre ou de déplacer l'appareil, retirez les lames du support de lame. Si des lames
restent à l'intérieur du support de lame, elles peuvent tomber sur des pièces internes de
l'équipement et causer des dysfonctionnements.
Conditions de
fonctionnement
Puissance de fonctionnement
100 - 240 VAC, 1.5 A
Fréquence
50 / 60 Hz
Entrée électrique
12 VDC, 5.0 A
Site d’installation
Utilisation en intérieur uniquement
Température de fonctionnement
10 - 35°C
Humidité relative maximale
10 - 80%
Altitude
≤ 2,000 m
Destruction de
l’instument
Suivez les règles et réglementations de votre gouvernement local.
Démontage de
l’instrument
Ne démontez en aucun cas l'instrument car cela invaliderait votre garantie.
Si l'instrument est endommagé ou fonctionne mal, contactez votre distributeur local ou Logos
Biosystems.
Sécurité personnelle

10
Consignes de
sécurité
Lisez et comprenez attentivement tous les manuels d'utilisation avant d'utiliser l'instrument.
Conservez tous les manuels d'utilisation dans un endroit sûr et accessible pour référence
future.
Lisez et comprenez toutes les fiches de données de sécurité avant de stocker, de manipuler
ou de travailler avec des réactifs.
Porter un équipement de protection individuelle (EPI) approprié lors de la manipulation des
réactifs et des échantillons cellulaires pour éviter toute exposition.
Lors de l'utilisation d'agents toxiques, de matières radioactives ou de micro-organismes
pathogènes appartenant aux groupes de risque 2 à 4 de l'OMS, respectez les lois et
réglementations nationales relatives aux exigences de niveau de biosécurité.
Cet instrument doit être entretenu par du personnel qualifié uniquement pour éviter les
blessures.
Traitement des
déchets
Ne réutilisez pas les lames jetables. Les lames usagées doivent être éliminées comme des
déchets biodangereux conformément aux règles et réglementations de votre gouvernement
local.

11
1. Product Introduction
Product Contents
Product Contents
The LUNA-FX7™ Automated Cell Counter is shipped with the following components.
Component
Quantity
LUNA-FX7™ Automated Cell counter
1
Power Cord with AC Adapter
1
Cell Counting Slides Sample
2 ea / 4 slide types
Trypan Blue Stain, 0.4 %
2 x 1 mL
Acridine Orange/Propidium Iodide Stain
2 x 0.5 mL
LUNA-FX™ Calibration Beads Kit
1
WiFi Dongle
1
USB Drive
1
Installation Guide
1
Quick Start Guide
1
Inspect the product package upon delivery to ensure that all components have been included.
Contact your local distributor or Logos Biosystems if anything is missing. Damage that may
occur during shipping and handling is not covered by warranty and must be filed with the
carrier.

12
Product Description
LUNA-FX7™
Automated Cell
Counter
The LUNA-FX7™ is an automated, image-
based cell counting device that features an
accurate counting algorithm and increased counting volume and represents
a fully automated
solution for cell counting and viability analysis. The LUNA-FX7™ also provides fl
exible
counting slide options from a single channel slide to a higher throughput, 8-channel slide.
Front view Right side Back
Touchscreen
The LUNA-FX7™ has a 7-inch capacitive touchscreen for navigating the user interface.
Slide port
The automated slide port enables one-time slide insertion.
Power button
The power button is used for the main power control.
USB ports
USB ports allow the user to transfer or print cell count data. Data may be transferred via
USB
drive or the provided WiFi dongle. Counting data may be printed using the LUNA-FX7™
Printer (P17001).
Ethernet port
The Ethernet port allows the instrument to be connected to a computer network.
The
CountWire™
software package enables automated data synchronization and the ability to
remotely operate the LUNA-FX7™.
Power inlet
Connect the power inlet of the instrument to an electrical outlet with the
supplied AC adapter
and power cord.
LUNA-FX7™ Cell
Counting Slides
The LUNA-FX7™ gives you the flexibility to use various counting slide formats. The LUNA-
FX7™ is compatible with the LUNA™ 1-, 3- , 8-Channel and Reusable Slide formats in
addition to the standard LUNA™ Cell Counting Slides and PhotonSlides™. With single-time
slide insertion, the LUNA-FX7™ is able to count all slide chambers at one time without needing
Slide port
USB ports
Power inlet
Ethernet port
To uc hs c r ee n
Power Button

13
to remove and reinsert a slide. The increased counting volume yields more accurate and
consistent results.
Channel No.
1 Channel
2 Channel
3 Channel
8 Channel
Reusable
Compatible
Slides
LUNA™
1-Channel
Slides
LUNA™
Cell Counting
Slides /
PhotonSlide™
LUNA™
3-Channel
Slides
LUNA™
8-Channel
Slides
LUNA™
Reusable slides
Sample
Throughput
1 sample
Up to
2 samples
Up to
3 samples
Up to
8 samples
1 sample
Sample
Loading
Volume
50 μL
10 μL/chamber
10 μL/chamber
10 μL/chamber
10 μL/chamber
Analysis
Volume
5.1 μL
1.3 μL/chamber
1.3 μL/chamber
0.5 μL/chamber
1.3 μL/chamber

14
2. Getting Started
Installation
Installation
Place the LUNA-FX7™ on a clean, level and sturdy surface.
o Avoid vibrations from other devices.
o Do not install the instrument in a location that will expose the device to intense
ultraviolet light.
o Allow at least 5 cm (2 inches) free space at the back of the instrument to prevent
overheating of the instrument.
o Allow at least 10 cm (4 inches) free space at the right of the instrument to insert/eject
a cell counting slide easily.
Connect the instrument to electrical outlets using the supplied power cord and AC adapter.
o Make sure the power cords are appropriate for your region.
o Always use power cord and AC adapter provided or approved by Logos Biosystems.
If appropriate cord is not used, the electrical safety of the instrument cannot be
guaranteed.
Connect the supplied WiFi dongle to a USB port.
(Optional) Connect a LUNA-FX™ Thermal Printer (P17001) to a USB port.
Setup
LUNA-FX7™
Startup
Push the power button located below the touchscreen to turn on the instrument. After
a short beep, the company logo will appear, followed by the home screen.
The home screen has three menus:
Brightfield Cell Counting
- Select Total cell counting mode or Cell counting & viability mode.
- Total cell counting mode is used to enumerate total cell numbers without staining ce

15
lls.
- Cell counting & viability mode is used to count cells and calculate the viability of cel
ls stained with Trypan Blue Stain, 0.4% (T13001) or Erythrosin B Stain (L13002) or y
east stained with Methylene blue Stain, 0.02% (L13004).
Fluorescence Cell Counting
- Select Cell lines & primary cells mode or Cell lines & primary cells, Advanced mod
e.
- Cell lines & Primary cells mode is used to count cells and calculate the viability of
cells stained with fluorescence dyes, Acridine Orange/Propidium Iodide Stain (F23001).
- Cell lines & Primary cell mode may also be used to count cells expressing GFP an
d/or RFP.
- Cell lines & Primary cells, Advanced mode is used to count cells and calculate the
viability of cells with an improved cell detection and cell de-clustering capabilities.
Quality Control
- Quality Control mode is only functional upon registration of Logos Biosystems brightfi
eld or fluorescent validation slides.
- The Quality Control menu is used to monitor the accuracy and variability of the instr
ument.
- The validation slides contain pre-spotted patterns or pre-fixed beads with a known c
oncentration and viability.
- Utilizing the Quality Control feature can provide daily, weekly, or monthly validation r
esults that may be graphically displayed, and/or downloaded.
Screen Saver
The screen backlight will automatically turn off after 10 minutes of inactivity. Touching
the screen will reactivate the instrument.

16
3. Counting Cells
Sample Preparation
Sample Staining
Brightfield cell counting
For Total and viability cell counting, prepare a cell suspension according to standard
procedures. Mix the sample, 1:1, with Trypan Blue Stain, 0.4% (T13001) or Erythrosin B Stain
(L13002) or Methylene blue Stain, 0.02% (L13004). Mix gently, but thoroughly to ensure a
homogenous suspension.
For total cell counting, load the sample directly onto the slide without staining the sample.
Fluorescent cell counting
Prepare a cell suspension according to standard procedures. Mix the sample, 9:1 (cells:
stain), with Acridine Orange/Propidium Iodide Cell Viability Kit (F23001). Mix gently, but
thoroughly to ensure a homogenous suspension.
Sample Loading
Load the appropriate volume for each slide chamber according to the table below:
LUNA™
1-Channel Slides
LUNA™ Cell
Counting Slides &
PhotonSlide™
LUNA™
3-Channel Slides
LUNA™
8-Channel Slides**
LUNA™
Reusable Slides
50 µL
10 µL
10 µL
10 µL
10 µL
** The LUNA™ 8-Channel Slides are multi-channel pipette compatible.
For easy and accurate loading, hold the slides by their edges and pipette at a 45-60° angle to
the slide. Take care not to overload or under-load the chamber.
Counting with the LUNA-FX7™
Slide Insertion &
Removal
Select appropriate counting mode and navigate to appropriate counting screen.
Press EJECT.
When inserting a slide into the instrument, ensure that the slide is facing up so that the arrow
is showing on the right side and/or so that the lowest chamber designation is to the left.
Insert from this side.

17
Press INSERT. The slide holder will automatically move into position.
To r emov e the slide, p ress EJECT. The slide holder will automatically extend out of the
instrument and the slide may be removed.
Viewing Images
By default, upon slide insertion, the viewing light will automatically turn on and the LUNA-
FX7™ will perform an initial autofocus. Whether or not autofocus is performed upon slide
insertion may be changed within SETTINGS (Section 8).
Light
By pressing the lamp icon in the bottom left corner of the screen, a light control panel will
appear. The intensity can be adjusted as needed. Press AE to use the auto-exposure function.
When in the fluorescence counting screen, the filters also may be switched between the BF,
Green, and Red channels. Photobleaching will occur with procolonged exposure, so work
appropriately.
Manual focus
Slide Port Control
Current FOV
Filter Switch &
Light Intensity
Slide Chamber Selection
Autofocus
Field of View Toggle

18
! Important ! Adjusting light intensity levels in the COUNT screen will only be applied
to the live view mode. Exposure levels for brightfield cell counting are automatically
adjusted. Exposure levels for fluorescence cell counting may only be adjusted within
a protocol (Section 5).
Focusing
To bring cells into focus, press Autofocus. Focus may also be adjusted manually using the
up & down arrows in the focus control bar.
Zoom
Zoom in or out by spreading or pinching two fingers. The outer box in the upper, left hand
corner of the viewing window represents the current field of view. The inner box represents
the view on the screen. Zooming in or out will cause the inner box size to decrease or increase.
Navigation
To view different slide chambers within a slide, select the chamber to be viewed by pressing
a chamber on the slide image just under the COUNT button. To see different fields of view
within a chamber, use the arrows located above the manual focus adjustment.
Cell Counting
Prior to counting, confirm that the image is in focus for the first field of view. When
the first
field is in focus, press the COUNT button.
The LUNA-FX7™ will count all slide chambers as designated in SETTINGS.
Counting time will vary depending on slide type, counting mode and the protocol used.

19
Results
Results
After counting is complete, the data and images will appear in the RESULTS window.
The counting results will be shown to the left of the screen.
Press the left or right arrows under Chamber to view the results and images for each counted
chamber.
Press TAG to identify live (green circles) or dead (red circles) cells.
After fluorescence cell counting, BF, GF, and RF images can be viewed separately or in
overlay.