logos biosystems LUNA FX7 Series User manual

User Manual
www.logosbio.com
LBSM-SM-ML-LU7-001
Rev. 6

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DISCLAIMER
The contents of this document are subject to change without notice.
The LUNA-FX7™ Automated Cell Counter is an electrical laboratory instrument for scientific research use only.
It is not a medical, therapeutic, or in vitro diagnostics device.
Do not disassemble the device on any occasion as this will invalidate your warranty.
TRADEMARKS
The trademarks used in this document are the property of Aligned Genetics, Inc.
©2021 Aligned Genetics, Inc. All rights reserved.
Logos Biosystems is the brand name of Life Science business of Aligned Genetics, Inc.

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CERTIFICATION MARKS
The WEEE (Waste Electrical and Electronic Equipment) symbol indicates that users of this
instrument have responsibility of returning and disposing of WEEE in an environmentally friendly
manner.
Follow the waste ordinances of your region for proper disposal provisions.
The CE mark indicates that this instrument conforms to all applicable European Community
provisions for which this marking is required. Users must be aware of and follow the conditions
described in this manual for operating the instrument. The protection provided by the instrument
may be impaired if the instrument is used in a manner not specified by this manual.
Protective earth (Ground)
This device complies with Part 15 of the FCC Rules.
This equipment complies with the requirement of UL 61010-1:2012, CAN/CSA C22.2 No.61010-
1:2012.
“Safety Requirements for Electrical Equipment for Measurement, Control, and Laboratory Use, Part
1: General Requirements.”
The KC certification mark indicates that this instrument conforms with Korea’s product safety
requirements for electrical and electronic equipment and components for which this marking is
required.

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Table of Contents
SAFETY PRECAUTIONS ·········································································································· 7
Instrument Safety ·················································································································· 7
General Safety ···················································································································· 7
Operating Conditions ············································································································ 7
Instrument Disposal ············································································································· 7
Instrument Disassembly ········································································································ 7
Personal Safety ····················································································································· 7
Safety Guidelines ················································································································ 7
Waste Disposal ··················································································································· 8
PRECAUTIONS DE SECURITE ··································································································· 9
Sécurité des instruments ······································································································ 9
Sécurité générale ··············································································································· 9
Conditions de fonctionnement ···························································································· 9
Destruction de l’instument ·································································································· 9
Démontage de l’instrument ································································································· 9
Sécurité personnelle ············································································································ 9
Consignes de sécurité ······································································································ 10
Traitement des déchets ···································································································· 10
1. PRODUCT INTRODUCTION ····························································································· 11
Product Contents ·················································································································· 11
Product Contents ··············································································································· 11
Product Description ··············································································································· 12
LUNA-FX7™ Automated Cell Counter ···················································································· 12
LUNA-FX7™ Cell Counting Slides ························································································ 12
2. GETTING STARTED ··········································································································· 14
Installation ·························································································································· 14
Installation ······················································································································· 14
LUNA-FX7™ Startup ·········································································································· 14
Screen Saver ···················································································································· 15
3. COUNTING CELLS ············································································································· 16

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Sample Preparation ·············································································································· 16
Sample Staining ················································································································ 16
Sample Loading ················································································································ 16
Counting with the LUNA-FX7™ ······························································································· 16
Slide Insertion & Removal ··································································································· 16
Viewing Images ················································································································· 17
Cell Counting ···················································································································· 18
Results ······························································································································· 19
Results ···························································································································· 19
Histograms ······················································································································· 20
Dilution Calculator ············································································································· 20
Save ······························································································································· 21
Print ································································································································ 22
Quick Save ······················································································································ 22
4. REVIEW ································································································································ 23
Reviewing Data ···················································································································· 23
Review Images ················································································································· 23
Reanalyze ························································································································ 24
Previous Counts ················································································································ 24
5. PROTOCOLS ······················································································································· 25
Protocol Selection ················································································································· 25
Default Protocol ················································································································ 25
Creating Protocols ············································································································· 25
Editing Protocols ·················································································································· 26
Load Protocol ··················································································································· 26
Protocol Parameters ············································································································· 27
Brightfield Cell Counting Parameters ····················································································· 27
Min./Max. search size ········································································································· 28
Cell detection sensitivity ······································································································ 28
Live cell sensitivity ············································································································· 28
Noise reduction ················································································································· 28
Dilution factor ··················································································································· 28
Fluorescence Cell Counting Parameters – Cell lines & Primary cells mode ···································· 28
GF/RF Exposure Level ······································································································· 29
Cell Size Calculation ·········································································································· 29
Min./Max. cell size ············································································································· 29

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GF/RF threshold level ········································································································· 29
Dilution Factor ·················································································································· 29
Fluorescence Cell Counting Parameters – Cell lines & Primary cells, Advanced mode ····················· 30
GF/RF Exposure Level ······································································································· 30
Min./Max. search Size ········································································································ 30
Declumping sensitivity ········································································································ 30
Min. FL intensity ················································································································ 31
Min. roundness ················································································································· 31
Dilution Factor ·················································································································· 31
6. BIOPROCESS FEATURE ·································································································· 32
Bioprocess Feature ··············································································································· 32
Bioprocess ······················································································································· 32
Creating a Bioprocess Protocol ···························································································· 32
Record Bioprocess Data ····································································································· 32
Review/Export Bioprocess Data ···························································································· 33
Graph ····························································································································· 33
7. QUALITY CONTROL ·········································································································· 34
Quality Control Mode ············································································································· 34
Quality Control ·················································································································· 34
Validation Slide Registration ································································································ 34
Performing Quality Control ·································································································· 37
Review ···························································································································· 37
8. SETTINGS ···························································································································· 39
LUNA-FX7™ Settings ············································································································ 39
Screen Settings ················································································································· 39
Cell Counting ···················································································································· 39
Save & Review ················································································································· 42
Network ··························································································································· 42
CountWire ························································································································ 44
Date & Power ··················································································································· 45
S/W & Calibration ·············································································································· 46
9. DATA TRANSFER VIA NETWORK ··················································································· 48
Network sharing ··················································································································· 48

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Connected to User PC ········································································································ 48
10. MAINTENANCE AND TROUBLESHOOTING ······························································· 51
Maintenance ························································································································ 51
Powering on/off ················································································································· 51
Cleaning ·························································································································· 51
Troubleshooting ··················································································································· 51
Inaccurate Cell Count ········································································································· 51
Slide Insertion ··················································································································· 52
Data Transfer and Saving ···································································································· 52
Software Update Errors ······································································································ 52
11. PRODUCT SPECIFICATIONS ························································································· 53
LUNA-FX7™ Automated Cell Counter ······················································································· 53
Physical and Technical Characteristics ··················································································· 53
LUNA™ Slides ····················································································································· 53
Physical Characteristics ······································································································ 53
12. ORDERING INFORMATION ···························································································· 54
Instruments ························································································································· 54
Slides and Reagents ············································································································· 54
CountWire™ ························································································································ 55
IQ/OQ ································································································································ 55
Accessories ························································································································· 55
13. PURCHASER NOTIFICATION ························································································ 56
Limited Use Label License ······································································································ 56
Research Use Only ············································································································ 56
Instrument Warranty ·············································································································· 56
Warranty ·························································································································· 56
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7
Safety Precautions
Instrument Safety
General Safety
Operate the instrument in the conditions described in the Operating Conditions.
Install the instrument on a level and sturdy surface. Avoid vibrations from other devices.
Do not touch components with wet hands.
Use components provided or authorized by Logos Biosystems. If the proper combination of
components is not used, product safety cannot be guaranteed.
Use only the power cord and AC adapter provided by Logos Biosystems. If the proper power
cord and AC adapter are not used, electrical safety of the product cannot be guaranteed.
Ensure that the input voltage is compatible with the power supply voltage of the product.
Connect the grounding terminal of the instrument and electrical outlet properly. If the
instrument is not grounded, electrical safety of the product cannot be guaranteed.
Turn on the instrument only after connecting the power cord and AC adapter to both the power
source and the instrument. Turn off the instrument before disconnecting the power cord and/or
moving the instrument.
Disconnect the power cord in the case of abnormalities.
Be careful with possible electric shock hazards as electric current may be still alive when the
instrument stops.
Do not hold the slide slot while it is in motion.
Protect USB drives from being infected with viruses and malware.
Before shutting down or moving the device, remove the slides from the slide holder. If slides
are left inside the slide holder, they may fall into the internal parts of the equipment and cause
malfunctions.
Operating
Conditions
Operating Power
100 - 240 VAC, 1.5 A
Frequency
50/60 Hz
Electrical Input
12 VDC, 5.0 A
Installation Site
Indoor use only
Operating Temperature
10 - 35°C
Maximum Relative Humidity
10 - 80%
Altitude
≤ 2,000 m
Instrument
Disposal
Follow the rules and regulations of your local government.
Instrument
Disassembly
Do not disassemble the instrument in any event as this will invalidate your warranty.
If the instrument is damaged or malfunctioning, contact your local distributor or Logos
Biosystems.
Personal Safety
Safety Guidelines
Read and understand all user manuals thoroughly before using the instrument.

8
Keep all user manuals in a safe and accessible place for future reference.
Read and understand all safety data sheets before storing, handling, or working with any
reagents.
Wear appropriate personal protective equipment (PPE) when handling reagents and cell
samples to avoid exposure.
When using toxic agents, radioactive materials, or pathogenic microorganisms belonging to
WHO Risk Groups 2-4, follow national laws and regulations for biosafety level requirements.
This instrument is to be serviced by trained personnel only to avoid injury.
Waste Disposal
Do not reuse disposable slides. Used slides must be disposed as biohazardous waste
according to the rules and regulations of your local government.

9
Précautions de sécurité
Sécurité des instruments
Sécurité générale
Faites fonctionner l'instrument dans les conditions décrites dans les conditions de
fonctionnement.
Installez l'instrument sur une surface plane et solide. Évitez les vibrations provenant des
autres appareils.
Ne touchez pas les composants avec les mains mouillées.
Utilisez uniquement les composants fournis ou autorisés par Logos Biosystems. En cas
d’utilisation d’une combinaison autre que celle qui a été recommandée, la sécurité du produit
ne peut être garantie.
Utilisez uniquement le cordon d’alimentation et l’adaptateur fournis par Logos Biosystems. En
cas d’utilisation du cordon et de l’adaptateur non appropriés, la sécurité electrique du produit
ne peut être garantie.
Assurez que la tension d’entrée est compatible avec la tension d’alimentation du produit.
Connectez correctement la borne de mise à la terre de l'instrument et la prise électrique. Si
l'instrument n'est pas mis à la terre, la sécurité électrique du produit ne peut pas être garantie.
Allumez l'instrument uniquement après avoir connecté respectivement le cordon
d'alimentation et l'adaptateur à la source d'alimentation et à l'instrument. Éteignez l'instrument
avant de débrancher le cordon d'alimentation et / ou de déplacer l'instrument.
Débranchez le cordon d’alimentation en cas d’anomalies.
Soyez prudent avec les risques d'électrocution, car le courant électrique peut être encore actif
lorsque l'instrument s'arrête.
Ne tenez pas le tiroir de lame lorsqu'elle est en mouvement.
Protégez les clés USB contre les virus et les logiciels malveillants.
Avant d'éteindre ou de déplacer l'appareil, retirez les lames du support de lame. Si des lames
restent à l'intérieur du support de lame, elles peuvent tomber sur des pièces internes de
l'équipement et causer des dysfonctionnements.
Conditions de
fonctionnement
Puissance de fonctionnement
100 - 240 VAC, 1.5 A
Fréquence
50 / 60 Hz
Entrée électrique
12 VDC, 5.0 A
Site d’installation
Utilisation en intérieur uniquement
Température de fonctionnement
10 - 35°C
Humidité relative maximale
10 - 80%
Altitude
≤ 2,000 m
Destruction de
l’instument
Suivez les règles et réglementations de votre gouvernement local.
Démontage de
l’instrument
Ne démontez en aucun cas l'instrument car cela invaliderait votre garantie.
Si l'instrument est endommagé ou fonctionne mal, contactez votre distributeur local ou Logos
Biosystems.
Sécurité personnelle