Medite USE 33 User manual

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
1
Ultrasonic Decalcifying Unit
USE 33
Installation & Operation Manual
IMPORTANT: Read this manual carefully before starting the instrument!
Rev. 05 (05/2007)
© Copyright: Duplication of this manual by any means for any purpose without
written consent of medite GmbH is prohibited.
Wollenweberstr. 12 - D-31303 Burgdorf - Tel: +49 5136 888 4 0 Fax: + 49 5136 888 4 55 [email protected] www.medite.de

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
Konformitätserklärung
Certificate of Conformity
Attestation de Conformité
Type of instrument:
Ultrasonic Decalcifying Unit
Model:
USE 33
Serial No.:
We do hereby certify that the above mentioned product meets the requirements set forth in the
CE Guidelines indicated below including all changes and addendums to date thereto. The
above mentioned product has been controlled by an authorized test center and meets the
following standards and guidelines:
EU Directive 98/37/EEC Machinery
EU Directive 89/336/EEC EMC
EU Directive 73/23/EEC Low Voltage
DIN EN ISO 9001
VDE 701
Burgdorf, 20.05.2007 M. Ott - Geschäftsführer
Wollenweberstr. 12 - D-31303 Burgdorf - Tel: +49 5136 888 4 0 Fax: + 49 5136 888 4 55 [email protected] www.medite.de

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
0
Contents
1.
General description
1
1.1 Safety instructions
1
1.2 Power supply
1
1.3 Ground wire connection
1
1.4 Maintenance and repair
1
1.5 Cleaning of the water circulation system
1
1.6Packing
2
2.
Installation
2
3.
Set up
2
4.
Instrument structure
3
5.
Operation
4
5.1 Cooling / Timer
4
5.2 Ultrasonic operation / Programmable timer
7
6.
Trouble shooting
10
6.1 Changing the fuse
11
7.
Technical specifications
11
8.
Accessory ( for separate order )
12
9
Application collection Use 33 2003-2004
13

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
1
1. General description
The following instructions for safety must be strictly observed to guarantee a safe
operation of the instrument. The same precautions considered when using any
technical equipment have to be observed. Failure to follow the recommended
procedure as outlined in this manual or misuse of the instrument will void the
manufacturer’s warranty.
1.1 Safety instructions
do not use the instrument without water
do not use inflammable liquids and chemicals containing or separating chloride
with the ultrasonic device
use aggressive chemicals only in the inset vessels
do not put anything on the bath ground
do not touch the liquid while ultrasound is in operation
keep the instrument’s surface and controls clean and dry
do not use for living beings
empty instruments before transporting them
during the ultrasonic operation cavitation noise is produced. In case of
continued presence in an area of 2 m around the instrument ear protections
shoud be worn to prevent from health damage.
1.2 Power supply
Before switching on the instrument check the compatibility of voltage and frequency
with the indications on the instrument label at the back of the unit.
Operating the instrument with a wrong voltage may cause damage!
1.3 Ground wire connection
As a prevention from electrical shock the instrument must be grounded. The unit is
supplied with a power plug with ground wire connection.
1.4 Maintenance and repair
Never remove parts of the casing during operation!
The replacement of parts and other interventions in the instrument may be done by
authorized service staff, only.
1.5 Cleaning of the water circulation system.
In order to avoid a clogging of the circulation system please follow this instruction
once every four weeks:
Take out the holder and let water drain off the bath. Remove coarse debris by a
cloth. Fill the bath and the tubing system with approximately 5 litres of cleaning agent

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
2
( DECON 90 about 5-10%, dissolved in water), switch on the pump and rinse the
tubing system with this solution and leave it in over night.
1.6 Packing
The packing of the ultrasonic decalcifying unit USE 33 contains:
USE 33
power cord
lid to cover the holders
user’s manual USE 33
2. Installation
After unpacking the instrument protecting films possibly existing on polished metal
surfaces must be removed. Inspect the instrument with regard to transport damages.
If transport damages are detected, do not connect the instrument to the power
supply. The bath should be cleaned thoroughly before starting operation.
The USE 33 is equipped with cooling slots on both sides. In order to provide
sufficient heat removal the instrument should be positioned in such a way that there
is a minimum distance of 30 cm from the wall. Furthermore, the unit must be level on
a dry and solid base.
3. Set up
1. Examine whether the drain tap is closed
2. Fill in tap water
3. Filling height: The water outlet must be completely covered with water.
4. When holders are equipped with vessels the water level should reach the lower
edge of the holder.
5. Vessels must not touch the bath ground!
6. Plug the power cord in the socket at the back of the instrument and connect to the
power supply (wall socket). Make sure that the drain tap is closed.
Operating conditions:
- the instrument must be used in closed rooms, only
- ambient temperature + 15°C to + 40°C(without considerable fluctuations)
- maximum height 2000 m ( according to EN 61010-1:2001)
- vibration free laboratory table adequate to the weight of the unit
- maximum relative humidity of 80%, not condensing for temperatures up to 31°C,
linear decreasing to 50% relative humidity at 40°C
- do not expose the unit to direct sunlight
- variation of supply voltage not higher than +/-10% of the nominal voltage

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
3
4. Instrument structure
Fig. 1: Front view USE 33
Fig. 2: Rear view USE 33
Power
connection with
fuse holder
Timer
Ultrasound
Temperature
control
cooling
Bath
Fig. 3: right side USE 33
Air outlet
Water outlet

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
4
Water outlet.
Minimum water level:
must be above the water outlet
5. Operation
The instrument can be switched to standby mode using the
switch on the rear of the instrument.
5.1 Controller Cooling / Timer
After switching on the instrument the actual temperature of the liquid is indicated in
°C on the display. Individual temperatures for the contents of the bath can be
adjusted by the temperature control.
Fig. 4: internal view USE 33

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
5
Features
Key 1: Up
This key is used to increase the temperature or time value, respectively.
Key 2: Down
This key is used to decrease the temperature or time value, respectively.
Key 3: SET
By pressing this key the mode is changed from SET temperature to SET
timer and standard mode.
Key 4: START/STOP
This key is used to start an endless operation or stop the timer operation if
the instrument is in timer mode.
KEY 5: TIMER START
This key is used to start or stop the timer
Key 4: Stand-by
This key is used to switch the instrument on and off, respectively (standby
mode)
Indication of temperatures above 45°C
No function in the operation of the USE 33
Indication: Cooling active
Indication: Fan and Ultrasound active

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
6
Set the temperature
The temperature value for the Ultrasonic Decalcifying Unit USE 33 is modified by
pressing the key SET once and then press the keys Up or Down.
Switch to temperature setting
(press 1x )
or
Adjust the desired value
If one of the keys Timer, Start/Stop or Standby is pressed, the indication on the
display immediately switches to normal mode. The newly set value becomes active
by releasing the keys. This does not interfere with a possibly activated running timer.
Set the duration time
The duration time for the Ultrasonic Decalcifying Unit USE 33 is set by pressing the
key SET twice and then pressing the keys Up or Down.
The value is adjustable from 0 up to 99 hours 59 minutes.
Switch to timer setting
(press 2x)
or
Adjust the desired value
If the timer value is changed while the timer is running, the changed value will
become effective only after the timer had been stopped or the time interval has
passed.
If one of the keys Timer, Start/Stop or Standby is pressed, the indication on the
display immediately switches to normal mode. The newly set value becomes active
by releasing the keys.

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
7
5.2 Ultrasonic operation / Programmable timer
Features
The timer is used to preset the duration time and to switch on or off the ultrasound,
respectively.
Key 1: UP
This key is used to increase the set time interval which is indicated when
the key SET TIME is pressed. During programming this key is used to go
to the next position.
Key 2: DOWN
This key is used to decrease the set time interval which is indicated when
the key SET TIME is pressed. During programming this key is used to go
to the previous position.
Key 3: SET TIME
This key is used to indicate a set time interval. In combination with the UP
or DOWN key the interval is changed accordingly. In the basic mode the
actual daytime can be corrected like this. In the programming mode this
key combination is used to change the start and stop time. Furthermore, if
also the key PROG is pressed while programming, the settings of the
previous day can be copied to the actual day.
Key 4: PROG
If this key is pressed and held for 5 seconds, you enter the programming
mode for the start and stop times. The programming always starts with
„Monday – start time –line 1.”
If also the key SET TIME is pressed, the settings of the previous day can
be copied to the actual day.

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
8
Key 5: ALL ON
This key is used to stop all control cycles. The timer settings are ignored
and the ultrasonic module is switched on. This mode is shown in the
display by the indication "ALL". Pressing this key again, the mode returns
to the programmed settings, whereas those lines which are
actually on remain on.
By switching the mains switch off and on, the instrument returns to the
programmed mode.
Key 6: STAND-BY
This key is used to switch the instrument to standby mode. If it is pressed
again, the active mode is restored. In the standby mode all lines are
switched off. The indication shows "OFF" and the LEDs are off.
By switching the mains switch off and on, the instrument returns to the
programmed mode.
Adjust actual day and time
If the SET TIME key is pressed while the instrument is in basic mode, the day time
can be changed by additionally pressing the key UP or DOWN, respectively. To
change the weekday go beyond midnight (24:00 hours). When all keys are released
the new adjustments for day and time will be stored.
+ or
Adjustment of the start and stop times
Having entered the programming cycle by pressing the key PROG for 5 seconds the
different positions are activated in the following sequence by pressing the UP or
DOWN key
Indication LED
ON1 Monday
OFF1
ON2
OFF2
ON3
OFF3
ON1 Tuesday
OFF1
ON2
OFF2
ON3
OFF3

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
9
ON1 Wednesday
OFF1
ON2
OFF2
ON3
OFF3
ON1 Thursday
OFF1
ON2
OFF2
ON3
OFF3
ON1 Friday
OFF1
ON2
OFF2
ON3
OFF3
ON1 Saturday
OFF1
ON2
OFF2
ON3
OFF3
ON1 Sunday
OFF1
ON2
OFF2
ON3
OFF3
ON1 Monday
OFF1...
After having reached the desired program position, by pressing the key SET TIME
the set time is indicated and can be changed by using the key UP or DOWN. When
all keys are released, the new value is stored.

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
10
Adjustment advice
The programming is made similar to that of an analogue timer, where one tag per
day can be set each for start and stop time , but it is not a must. The endless loop in
the programming cycle enables a quick access to the weekend days Saturday and
Sunday by pressing the DOWN key.
Please note that there is no action if the start and stop time for the same day are
identical.
Between the times 23:59 and 0:00 there is the special position "----". This adjustment
deactivates the time setting. It is used to leave the instrument on during the day
change or to keep it in on or off mode during several days.
If during programming the PROG key is pressed while the SET TIME key is held
after a day has been chosen, all times set for the previous day are copied to the
chosen day and the display shows "COPY".
Return to basic mode
When the PROG key is pressed for at least 3 seconds or all other keys are released
for more than 60 seconds, the instrument returns to basic mode.
Error codes and displays
Display
Reason
Action
rC
Loss of data in timer
Adjustment of timer
EP
Loss of data in memory
Repair of controller
ALL
Modus "On",
all outputs active
-----
6. Trouble shooting
At the rear of the instrument there is the power socket with integrated fuse holder.
In case of a non-functioning of a unit, replace the fuse. The correct fuse parameters
are shown on the instrument label.
In case of any other defect, please contact the
service department.
Please do not try to repair the instrument
yourself. Unauthorized modification or repair by
third party persons will void warranty.
6.1 Changing the fuse

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
11
7. Technical specifications
Ultrasonic decalcifying unit USE 33
Width
:
450 mm
Depth
:
600 mm
Hight
:
350 mm
Filling capacity
:
13,5 litres
Cooling
temperature
:
14°C to 25°C 1°C
Weight
:
30 kg
Power supply
:
230 V / 50 Hz or 115 V / 60 Hz
400 VA
Fuse
:
4 A
8. Accessory ( for separate order )
First pull out the mains plug !
Using a screw driver it is easy to open the fuse socket and
to change the fuse.
Fuse socket

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
12
Description:
Cat. No.:
Holder for glass jars for USE 33
Holder for specimen, no. 1
Qty. of specimen: 4
Specimen container size: d = 93 mm
Height: 110 mm
Container volume: approx. 800 ml.
Max. size of specimen : 250 cm3
28-8252-00
Holder for glass jars for USE 33
Holder for specimen, no. 2
Qty. of specimen 9
Specimen container size: d = 51 mm
Height: 85 mm
Container volume: approx. 150 ml
Max. size of specimen: 30 cm3
28-8253-00
Holder for plastic pots for USE 33
Holder for specimen, no. 3
Qty. of specimen: 49
Specimen container size: d = 25 mm
Height: 40 mm
Container volume: approx. 20 ml
Max. size of specimen: 5 cm3
28-8254-00

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
0
Collection of USE 33 procedures. These procedures are compiled results of our users. Recommended common temperature range for decalcification of all samples is 16°C to 17°C. This
temperature range can be adjusted in the USE 33.
Application/
Tissue
Sample
Weight/Size
Type of decalcifying reagent
Condition:
Tested staining and
IHC reagents
Comment & Error
bone marrow
biopsies
Weight:
approx.. 0,5 - 0,8 g
Length: 8 - 17 mm
: 0,2 - 0,6 mm
Single (isolated independently treated samples),
(plastic vial), Insert No.3
Reagent: 20 ml USEDECALC®
Reaction time in USE 33: 8 - 17 h
Additional treatment:
Optional running water or washing 5-10 min with
fresh water in the USE 33
HE, Giemsa, CD 61, ASDCL
(Chloracetat-Esterase); Gomori
Silverimpregnation AG NOR, Berlin
blue (FE), Hematoxilin Eosin
(Hemalaun)
IHC and Protease
s. Ref. 1 & 5
Fine morphological and
immunhistological results.
The data are based on 50 to 80
investigations within 2002 to 2003
Possible or known error: none
cartilage tissue
Weight up to 1,5 g
Length: 10 - 30 mm
: 10 mm
Single (isolated independently treated samples),
(plastic vial), Insert No.3
Reagent: 20 ml USEDECALC®
Reaction time in USE 33: 24 h
Additional treatment*):
Optional running water or washing 5-10 min with
fresh water in the USE 33
HE, Giemsa, CD 61, ASDCL
(Chloracetat-esterase); Gomori
Silberimpregnation, Berlin blue (FE),
Hematoxilin Eosin (Hemalaun)
Fine morphological and
immunhistological results.
Possible or known error: none
cartilage tissue
Weight up to 2,5 g
Length: ca. 20 - 35 mm
Width: 5 bis 10 mm
Heigth: 5 bis 10 mm
Insert No. 1 (46-1026-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 3 - 18 h
Additional treatment: neutralize within approx. 1 h
using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh water
in the USE 33)
HE, Giemsa, CD 61, ASDCL
Gomori Silberimpregnation, Berliner
Blau, Hematoxilin Eosin (Hemalaun)
Fine morphological and
immunhistological results.
Possible or known error:
ASDCL: a weak stain might occur in
dependence of the length of the
reaction time.
Full slice of
Ilium head
Weight: not
documented
: 20 - 35 mm
thickness: 2 - 5 mm
Insert No. 1 (46-1026-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 24h
Additional treatment: neutralize within approx. 1 h
using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh water
in the USE 33)
HE, Giemsa, CD 61, ASDCL
Gomori Silberimpregnation, Berlin
blue (FE), Hematoxilin Eosin
(Hemalaun)
Good and reproducible morphological
and immunhistological results.
Possible or known error:
ASDCL: a weak stain might occur in
dependence of the length of the
reaction time.
In case the sample is not satisfactorily
decalcified „Rapid Block Post
Decalcification Procedure“:
where appropriate, trim sample
(Paraffin block) and shortly decalcify
the block within 5 -10 minutes in the
USE beaker with USERAPID®
followed by a 2-3 minute
neutralization in USEDECALC® ..
USE Application USE 33 2003-2004
13
9

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
1
Application/
Tissue
Sample
Weight/Size
Type of decalcifying reagent
Condition:
Tested staining and
IHC reagents
Comment & Error
Incisor
(human)
Weight: 1,2 –2 g
Insert No. 1 (46-1026-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 24-48h
Additional treatment: neutralize within approx. 5 –
30 min using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh water
in the USE 33)
HE, Giemsa, CD 61 Gomori
Silverimpregnation, Berlin Blue,
Hematoxilin Eosin (Hemalaun)
See Ref. 3
„Rapid Block Post Decalcification
Procedure“:
where appropriate trim sample
(Paraffin block) and decalcify the
block within 20 -30 minutes in the
USE beaker with USERAPID®
followed by a 2-3 minute
neutralization in USEDECALC® .
bone marrow
biopsies
Weight ?
Length 8 bis 12 mm
: ca. 0,2 mm
Insert No. 1 (46-1026-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 1 h
Additional treatment: neutralize within approx. 3 h
using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh water
in the USE 33)
HE, Giemsa, ASDCL,
CD3, CD79a, CD 34, MPO
IHC Reagents from DAKO
CYTOMATION
See Ref. 2
Approx.. 50 samples had been
investigated
The short reaction time of
USERAPID®showed good and
reproducible results for IHC and
ASCDL (Chloracetic-esterase)
.
Incisor
(human)
Weight: ca. 1,2 - 2 g
Insert No. 1 (46-1026-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 3 d
Additional treatment: neutralize within approx. 5-30
min using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh water
in the USE 33)
Alcian Bue,
Immunhistochemie IGF II
s. Ref. 3
Decalcification procedure allows an
ideal morophological and
immunhistochemical follow up
Incisor
(human)
Weight: ca. 1,2 - 2 g
Insert No. 1 (46-1026-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 11 d
Additional treatment: neutralize within approx. 3d
min using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh
water in the USE 33)
PAS
Hard material,
in laboratory routine these samples
need a decalcification time of several
weeks with RDO®.
With other methods these samples
are not accessable.
Lower jaw/
mandible
(Rat)
Weight: ?
Length:
Insert No. 2 (46-1027-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 7 d
Additional treatment: neutralize within approx. 3 d
min using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh water
in the USE 33)
where appropriate „Rapid Block Post
Decalcification Procedure“
HE, Giemsa, Immunhistochemie
ED1 Osteoklasten,
Osteoid HE, Alcianblau
s. Ref. 3
Time is not optimised. A faster
procedure is in validation.
Dendrite,mucosa,
Osteocyte, Predontoblaste
Osteoclaste, dendriteskin,
longitudinal and cross sections
through teeth, Desmodont bone
14

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
2
Application/
Tissue
Sample
Weight/Size
Type of decalcifying reagent
Condition:
Tested staining and
IHC reagents
Comment & Error
Block from
ileum
cortical >90%
Weight ca. 0,5 g
Insert No. 1 (46-1026-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 2-3 d
Additional treatment: neutralize within approx. 3 h
using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh water
in the USE 33)
where appropriate „Rapid Block Post
Decalcification Procedure“
HE, Giemsa, KL1, MIB1, Vimentin,
S100
Results from systematic optimization
trials
Often the samples had not been
„cuttable“ after 14 days decalcification
with EDTA, only.
Missing: The proof of RNA
conservation was not investigated
systematically.
Block frum
ileum
cortical approx.
>50%
Weight ca. 0,5 g
Insert No. 1 (46-1026-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 1 h
Additional treatment: neutralize within approx. 4 -6 h
using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh water
in the USE 33)
where appropriate „Rapid Block Post
Decalcification Procedure“
HE, Giemsa, KL1, MIB1, Vimentin,
S100
s. Referenz 4
Results from systematic optimization
trials.
Often the samples had not been
„cuttable“ after 24 hours
decalcification with EDTA, only.
DNA conservation is comparable
towards „standard lab EDTA“
processing.
bone marrow
biopsies
base material
for
„Histo-Chips“
or Multi Tissue
Array
Weight: ca. 0,5 - 0,8 g
Length: ca. 8 - 17 mm
: ca. 0,2 - 0,6 mm
Insert No. 1 (46-1026-00, beaker made of glass)
with approx. 40 ml USERAPID®
Reaction time: 1-2 h
Additional treatment: neutralize within approx. 2 -6 h
using USEDECALC®optional (followed by
running water or washing 5-10 min with fresh water
in the USE 33)
where appropriate „Rapid Block Post
Decalcification Procedure“
RA, RAEB, Leder‘s Stain, CD34, Ki-
67, bcl-2
s. Referenz 5
„Rapid Block Post Decalcification
Procedure“ is a possible and
recommended opportunity for „Histo
Chips“
- for instance after EDTA operation in
case the block cannot be cut on the
microtome.
„Rapid Block Post Decalcification Procedure“:where appropriate cut sample (Paraffin block) and decalcify the block within 5 -10 minutes in the USE beaker with USERAPID®
followed by a 2-3 minute neutralization in USEDECALC® .
15

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
3
A compilation of applications for higher volume samples like animal (dog, rat) skull including a validation for staining and IHC-procedures is in preparation.
Figure: Histo Chips from bone marrow biopsies. Thanks to the kind permission of
Prof. Hansmann and Mr. Lieberz Universität Frankfurt 5)
References & Results of tests:
1)Techniken und Methoden der Histo- und Immunfärbung an
entkalkten Gewebeproben, Applikationen für den medite USE 33
Ultraschall-Schnell-Entkalker mit gewebeschonender USEDECALC
Entkalkerlösung, Herr Prof. Hansmann, Herr Lieberz, Universität
Frankfurt, medite Broschüre 2003
2) Beurteilbarkeit von Knochenpunktionszylindern nach
unterschiedlicher Fixation und identischer Entkalkung im USE 33, Dr.
Knolle, Frau Dr. Hege, Herr Balthasar, Klinikum Dessau.
3) Prüfung der Eignung des USE 33 für den Einsatz an der Poliklinik
für Kieferorthopädie Bonn, Prof. Götz, Frau Beil.
4) Entkalkung mit dem Ultraschall für immunhistologische Nachweise,
MHH, Dr. Länger, Frau Lehne.
5) Analysis of differential protein expression in myelodysplastic
Syndromes using histo-chips, Engels .K, Kriener .S, Lieberz .R,
Hansmann .ML, Senckenberg‘s Institute of Pathology, University
Frankfurt/M., Germany
Thanks to the kind permission and support to all who assisted.
This application was issued by
medite Medizintechnik GmbH
Wollenweberstr. 12
D-31303 Burgdorf
RAEB
Leder‘s
Stain
Ki-67
CD34
bcl-2
RA
normal
Magnification of all micrographs: 160x
Figure 4: Staining results
USE Application USE 33 2003-2004
16

Rev. 05 (01/2006) - SE - USE33E_Druck05.DOC
0
medite –Supplier of complete systems for histology-cytology-pathology
e. g. paraffin embedding system TES 99
Cooling Unit
TES 99.410
Width : 500 mm
Depth : 570 mm
Height : 300 mm
Cooling plate : 500 x 260 mm
Temperature : adjustable from +15° C to -15° C
Weight : 25 kg
Voltage : 230V / 50Hz / 320VA
TES 99.420
Width : 360 mm
Depth : 570 mm
Height : 300 mm
Cooling plate : 360 x 260 mm
Temperature : adjustable from +15° C to -10° C
Weight : 20 kg
Voltage : 230V / 50Hz / 150VA
TES 99.430
Width : 360 mm
Depth : 570 mm
Height : 300 mm
Cooling plate : 210 x 260 mm
Temperature : adjustable from +15° C to -10° C
Weight : 20 kg
Voltage : 230V / 50Hz / 150VA
Dispenser Unit TES 99.200
Width : 360 mm
Depth : 570 mm
Height : 300 mm
Working plate : 360 x 245 mm
Temperature : adjustable fom + 30° C to + 70°C
Capacity of paraffin tank : 3,5 litres
Cold point : -5° C 40 mm Ø
Heating for 6 forceps
Weight : 22 kg
Voltage : 230V / 50Hz / 350VA
Thermal Unit TES 99.600
Width : 360 mm
Depth : 570 mm
Height : 300 mm
Preheating : 305 x 205 mm
Warming tray : 295 x 235 mm
Weight : 15 kg
Voltage : 230V / 50Hz / 750VA
Wollenweberstr. 12 - D-31303 Burgdorf - Tel: +49 5136 888 4 0 Fax: + 49 5136 888 4 55 [email protected] www.medite.de
16
Table of contents
Other Medite Laboratory Equipment manuals