CURIOSIS FACSCOPE B User manual

Automatic Cell Counter
Instruction manual

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Developed and manufactured by CURIOSIS Inc.
E-mail: inf[email protected]o.kr
Website: http://www.curiosis.com
CURIOSIS Inc.
10 Teheran-ro 38-gil, Gangnam-gu, Seoul, Republic of Korea
Tel: [phone]
Fax: [phone]
Copyright  2019, by CURIOSIS Inc.
All rights reserved. Published in Korea.

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Package contents
FACSCOPETM B Automatic cell counter package includes the following items.
Item Quantity
FACSCOPETM B main device 1
Instruction manual 1
Quick manual 1
Main power cable 1
USB memory 1
FACSCOPE Slide (for 200 tests, Optional) 50 ea. per a box
Keypad (Optional) 1
Barcode scanner (Optional) 1
Thermal printer (Optional) 1
When receiving the package,
• Check that all items listed above are included in your package.
• Examine the device carefully for any damage during shipping.
• Contact your local distributor or [email protected] if any items are missing or damaged.
• Any loss or damage claims must be filed with the carrier.

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Safety instruction
READ ALL INSTRUCTION BEFORE USE
Caution
• Check the power supply input voltage and do not plug in when the voltage is unmatched.
• Check the power cable is connected to a grounded and 3-conductor power wall outlet.
• Check the power cable is properly grounded to avoid potential electrical shock.
• Check the main power switch is off when plugging in the power cable to the wall outlet or
unplugging the power cable.
• Wait about 2-3 minutes for the device to reboot.
• Do not insert any metallic objects into the device through backside air vent to avoid electrical shock
causing personal injury or device damage.
• Place the device around 10 cm away from the surroundings for proper air-cooling.
• Do not disassemble the device and contact an authorized service center if required.
• Use authorized accessories only.
• Operator should have the general knowledge of cell counting procedure and handling bio samples
safely.
• Operate the device carefully as described in this manual.
Warning
• Battery
Lithium battery is inside the device. Replacing it with incorrect type can cause risk of explosion. Do
not replace by a user and contact an authorized service center if required.
• Sample handling
Sample may contain the infectious biohazardous substances. Operator should wear gloves while
sampling.
• Waste
Dispose used FACSCOPE Slides as biohazardous waste and do not reuse them.

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Product specifications
FACSCOPETMB
Voltage AC 100~240 V, 50~60 Hz
Current Max. 1.0 A, 50 W
Objective lens 4 x
Light source 4 W Green LED
Camera
5Mega pixels high resolution
monochrome CMOS image
sensor
Weight 5 Kg
Size (W × L × H) 163 × 293 × 216 mm
Measuring
concentration range 1 x 104 ~ 1 x 107 cells/mL
Detectable cell
diameter 5 ~ 60µm
Measuring speed*
Quick mode: ≈ 20s per test
Normal mode: ≈ 30s per test
Precise mode: ≈ 100s per test
Counting area
Quick mode: ≈ 0.15 µL
Normal mode: ≈ 0.9 µL
Precise mode: ≈ 3.6 µL
FACSCOPE
Slide
(Cat. No.
CRFCB-CSD50)
(Optional)
Quantity 50 slides (for 200 tests)
Sample loading
volume 20 µL
Accessories
(Optional)
Power cable 1.5 m
USB memory Support USB 2.0
(Optional)
Keypad or
Barcode scanner or
Thermal printer
USB type

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*Cell Counting time is variable according to cell type and concentration.
Device layout
Frontal view
• Slide holder door – Slide holder is ejected from / inserted into the device.
• Touch LCD display – Preview, automatic cell counting processes and the results are displayed.
• 3 control buttons
Slide holder door
Touch LCD display
Button 3(Setting screen)
Button
1(Insert/Eject
slide holder)
Button 2(Home screen)

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Rear view
• 3 USB ports – Keypad, Barcode scanner, Thermal printer (optional), or USB memory are
connected to these ports.
• Ethernet port – LAN cable is connected to this port for PC interface.
• Power switch – Main device power ON/OFF control.
• Power cable socket – Power cable is connected to this socket.
USB Port
- Keypad
- Barcode Scanner
- Thermal Printer
- USB Memory
Ethernet Port
- PC Interface
Power Switch
Power Cable Socket

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Table of contents
Package contents 3
Safety instruction 4
Product specifications 5
Device layout 6
Introduction
FACSCOPETM B – Automatic cell counter 10
FACSCOPE Slide (50 slides for 200 tests per a box, Cat. No. CRFCB-CSD50)
Getting started
Pre-requirements 12
Basic installation 13
Power up and initial display 14
General operation
Sample preparation 15
Basic operation 16
Preview prior to counting 18
Making a stop while counting 20
Set counting option 21
A. Changing user group 22
B. Setting Count mode 23
C. Creating Preset 24
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5
6
10
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21
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23
24

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D. Editing Preset 27
E. Selecting Channel 29
F. Entering Channel ID 30
On the result screen 35
A. Analyzing by Histogram 36
B. View result image 38
C. Printout Cell Count Result using Thermal Printer 40
D. Exporting the Report into USB memory 41
E. Exporting Data (all history) into USB memory 43
F. Showing ID 46
On the Setting screen 47
A. Checking Firmware information and Update Firmware
48
B. Bead Quality Control 50
C. Setting Time 52
Maintenance and cleaning 53
Appendix
A. Trouble shooting 54
B. Examples of error and inaccurate result 55
C. The contents of result data 57
D. Example and explanation of PDF report 58
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Introduction
FACSCOPETM B – Automatic Cell Counter
FACSCOPETM B is a fully automated cell counting system based on a brightfield microscopy technique
for mammalian cell counting. The FACSCOPETM B utilizes high-powered LED light source, CMOS image
detection (5 Mega pixels), precise X-Y-Z stages and on-slide image processing technologies for fast
and accurate cell analysis.
Cell counting using FACSCOPETM B is done by 3 steps, (1) cell staining, (2) loading sample, and
(3) counting. Cells are mixed with trypan blue dye to distinguish between live and dead cells. The
stained sample is pipetted on the disposable plastic slide (4 tests per 1 slide) and the slide is loaded
into the FACSCOPETM B. After loading the slide, optic system automatically focuses on the slide and
then the FACSCOPETM B acquires and analyzes images automatically. The precise X-Y-Z stages move
through the preset routes to take multiple images for each channel. Highly sensitive CMOS sensor
acquires bright-field microscopy images and sends images to the integrated system for image
processing and analysis. The whole counting process takes 2 minutes (in Normal mode) and the
counting results are displayed on the LCD touch screen panel in front of the instrument.

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FACSCOPE Slide (50 slides for 200 tests per a box, Cat. No. CRFCB-CSD50)
FACSCOPE Slide is a disposable plastic hemocytometer having 4 channels engraved with Neubauer
Improved pattern. Each channel has an enclosed structure of 100um depth and hydrophilic surface.
The precise capacity and diffusible surface make cells evenly distributed and lead to accurate analysis.
FACSCOPE Slide can be used for mammalian cell counting with automatic cell counter FACSCOPETM
B, as well as manual counting method. Measuring range of cell concentration is 1 x 104 ~ 1 x 107 per
mL when using with FACSCOPE B.
For cell counting, prepare cell suspension for counting and mix cell suspension with trypan
blue at a one to one ratio. A channel of FACSCOPE Slide is filled with 20 μL of mixture and is loaded
into the FACSCOPETM B. After the analysis completion, the results will be displayed.
Keep FACSCOPE Slide box upright and at room temperature. It should be used immediately
after unsealing. Follow the exact procedure detailed in the Instructions for Use section.

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Getting started
Pre-requirements
For normal and stable operation of the device, the following environmental conditions should be
satisfied.
• Room temperature between 20 ~ 35 °C.
It is not recommended to operate the device at low temperature condition (below 10 °C)
In those conditions, warm up the device for over 10 minutes.
• Relative humidity between 0 ~ 95 %.
• Place at corrosive gases-free or other substances-free area.
• Place at dust-free or other airborne particles-free area.
• Avoid direct sunlight, vibration, magnetic or electromagnetic fields.
• Do not put any heavy materials on the top of the device.

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Basic installation
1. Unbox FACSCOPETM B p
ackage and place the
device on a flat and dry surface.
2. Plug accompanying power cable into the
power cable socket.
3.
Connect optional peripherals (keypad,
barcode scanner, or thermal printer) to the
USB port if necessary.
4. Switch on the power switch.
Check that the main power switch is I
(ON)
position.

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Power up and initial display
1. Once the main power is switched on,
boot image is displayed on the LCD
touch
s
creen. When booting is completed,
initializing process starts
and internal
motorized stages start moving.
2. Initializing progress is displayed while
processing.
3. When initializing is finished,
the slide
holder is ejected, and Home screen
is
displayed on the LCD touch screen.
4. After load a slide with sample
, the device
is ready to count.

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General Operation
Sample Preparation
Cell suspension, 0.4% trypan blue, micro tube 1.5ml, pipette, tips, and FACSCOPE Slide are necessary
for cell counting. Whole procedure should be done in clean area to avoid decreasing accuracy of
counting due to dust.
STEP 1. Prepare necessary items.
STEP 2. Place 20 μL of trypan blue in the micro tube and add an equal volume of the cell
suspension.
NOTE: Before sampling the cell suspension, gently resuspend the cells at least 6 times
(pay attention to avoid bubbles and check if there are any cell clumps or agglomerates)
The sampling should be in middle of the cell suspension, not on the surface or the bottom.
STEP 3. Mix the sample in the micro tube by pipetting the vial 3~5 times gently.
NOTE: Be careful not to bubble.
STEP 4. Load 20 μL of the stained cell sample onto each channel of FACSCOPE Slide.
NOTE: The sampling should be in middle of the cell suspension, not on the surface or the bottom and
ensure that no bubbles enter the channel.

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Basic Operation
STEP 1. Insert FACSCOPE Slide loaded with the sample onto the slide holder.
NOTE: Make sure the arrow on the slide points toward the instrument.
STEP 2. Press Start button to start counting procedures. The slide holder is inserted
automatically, and auto-focusing is performed prior to counting each sample.

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STEP 3. Counting progress is indicated as shown in following image. For completion of each sample,
the count result (unit: x104/mL) are displayed.
STEP 4. Once counting is complete, the slide holder is ejected automatically. Remove FACSCOPE Slide
from the slide holder.
1
1
Home
260 40 15
340 140 41
500 420 84
200 100 50

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Preview prior to counting
In the screen where you can see the cells, when tap the screen twice, some icons disappear.
To get icons again, tap the screen twice.
STEP 1. Load a slide and press Preview button.
STEP 2. Select a channel to preview.
STEP 3. Positioning and Autofocusing

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STEP 4. See the cell image of the selected channel.
STEP 5. If press Mark , the detection mark is displayed according to the selected preset.
Live/Dead definition can be modified at this stage.
STEP 6. Counting
1

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Making A Stop While Counting
STEP 1. To make stop while counting, Press STOP button.
STEP 2. Confirmation message box is displayed as shown the following image.
Press Continue button.
STEP 3. Once making a stop is confirmed, all remaining process are stopped, and the slide holder is
ejected automatically.
1
1