Metrohm 889 User manual

889 IC Sample Center
Manual
8.889.8001EN

Metrohm AG
CH-9101 Herisau
Switzerland
Phone [phone]
Fax [phone]
[email protected]
www.metrohm.com
889 IC Sample Center
Manual
8.889.8001EN 02/2010 dm

Teachware
Metrohm AG
CH-9101 Herisau
[email protected]
This documentation is protected by copyright. All rights reserved.
Although all the information given in this documentation has been checked
with great care, errors cannot be entirely excluded. Should you notice any
mistakes please send us your comments using the address given above.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents
889 IC Sample Center ￭￭￭￭￭￭￭￭ III
Table of contents
1Introduction 1
1.1 Instrument description ......................................................... 1
1.1.1 Model versions ........................................................................ 1
1.1.2 Instrument components .......................................................... 2
1.1.3 Intended use ........................................................................... 2
1.2 About the documentation ................................................... 2
1.2.1 Symbols and conventions ........................................................ 2
1.3 Safety instructions ................................................................ 3
1.3.1 General notes on safety ........................................................... 3
1.3.2 Electrical safety ........................................................................ 3
1.3.3 Tubing and capillary connections ............................................. 5
1.3.4 Personnel safety ...................................................................... 5
1.3.5 Flammable solvents and chemicals ........................................... 6
1.3.6 Recycling and disposal ............................................................. 6
2Overview of the instrument 7
2.1 Front and rear ....................................................................... 7
2.2 Opening the device ............................................................... 8
2.3 Interior view ........................................................................ 10
2.4 Interior ................................................................................. 11
2.5 Cooling option .................................................................... 12
3Installation 13
3.1 Setting up the instrument .................................................. 13
3.1.1 Packaging .............................................................................. 13
3.1.2 Checks .................................................................................. 13
3.1.3 Location ................................................................................ 13
3.1.4 Removing the device from the packaging .............................. 13
3.2 Connecting a computer ...................................................... 14
3.3 Connecting pump and column .......................................... 15
3.4 Tubing ................................................................................. 16
3.4.1 Tubing guide ......................................................................... 16
3.5 Outlet tubings ..................................................................... 17
3.6 Rinsing the syringe ............................................................. 18
4Functioning 19
4.1 Injection modes .................................................................. 19
4.2 Syringe and buffer loop ..................................................... 20

Table of contents ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
IV ￭￭￭￭￭￭￭￭ 889 IC Sample Center
4.3 Full loop injection ............................................................... 21
4.4 Partial loopfill injection ...................................................... 24
4.5 Pickup injection .................................................................. 27
5Handling and maintenance 31
5.1 General ................................................................................ 31
5.2 Care ...................................................................................... 31
5.2.1 Cleaning in general ................................................................ 31
5.3 Maintenance and service ................................................... 32
5.3.1 Sample loop .......................................................................... 32
5.3.2 Replacing the sample needle ................................................. 33
5.3.3 Replacing the air needle ........................................................ 35
5.3.4 Replacing the fuses ................................................................ 36
5.4 Quality Management and validation with Metrohm ....... 37
6Troubleshooting 38
6.1 Error list ............................................................................... 38
6.1.1 Rack unit ............................................................................... 38
6.1.2 Needle unit ............................................................................ 39
6.1.3 Syringe unit ........................................................................... 41
6.1.4 Injection valve unit ................................................................. 42
6.1.5 Cooling unit .......................................................................... 42
6.1.6 Electronics ............................................................................. 43
6.2 Analytical problems ............................................................ 43
6.2.1 Autosampler .......................................................................... 43
7Appendix 46
7.1 Samples and sample vials .................................................. 46
7.2 I/O interface ........................................................................ 46
7.2.1 Properties of the I/O interface ................................................ 47
7.2.2 Pin assignment of the remote interface .................................. 47
8Technical specifications 48
8.1 General ................................................................................ 48
8.2 Sampling .............................................................................. 48
8.3 Analytical characteristics ................................................... 49
8.4 Programming ...................................................................... 49
8.5 Interfaces ............................................................................. 49
8.6 Options (pre-installed) ........................................................ 49
8.7 Mains connection ............................................................... 50
8.8 Safety specifications ........................................................... 50

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ Table of contents
889 IC Sample Center ￭￭￭￭￭￭￭￭ V
8.9 Electromagnetic compatibility (EMC) ................................ 50
8.10 Ambient temperature ......................................................... 51
8.11 Dimensions .......................................................................... 51
9Conformity and warranty 52
9.1 Declaration of Conformity ................................................. 52
9.2 Warranty (guarantee) ......................................................... 53
9.3 Quality Management Principles ........................................ 54
10 Accessories 56
10.1 Scope of delivery 2.889.0010 ............................................ 56
10.2 Scope of delivery 2.889.0020 ............................................ 58
10.3 Optional accessories 2.889.0010 ...................................... 59
10.4 Optional accessories 2.889.0020 ...................................... 60
Index 61

Table of figures ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
VI ￭￭￭￭￭￭￭￭ 889 IC Sample Center
Table of figures
Figure 1 889 IC Sample Center - front ............................................................. 7
Figure 2 889 IC Sample Center - rear .............................................................. 8
Figure 3 Interior view without covering ......................................................... 10
Figure 4 Interior with sampling device ........................................................... 11
Figure 5 Interior with installed cooling option. .............................................. 12
Figure 6 Unpacking the 889 IC Sample Center .............................................. 13
Figure 7 Connecting pump and column ........................................................ 15
Figure 8 Guiding the rinsing tubing ............................................................... 16
Figure 9 Tubing guide from above ................................................................ 17
Figure 10 Mounting the outlet tubing ............................................................. 17
Figure 11 Mounting the leakage tubing .......................................................... 17
Figure 12 PASA™ injection concept ............................................................... 19
Figure 13 Full loop injection ............................................................................ 21
Figure 14 Replacing the sample needle ........................................................... 33
Figure 15 Pin assignment of I/O socket and plug ............................................. 47

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 1 Introduction
889 IC Sample Center ￭￭￭￭￭￭￭￭ 1
1 Introduction
1.1 Instrument description
The 889 IC Sample Center is a robust Autosampler for high sample through-
put, optimized for the challenges of the modern analytic laboratory. Its
greatest advantages are speed and small sample volumes.
Special features:
￭Control of syringes with high resolution. This enables very high precision
during injections.
￭The control per PC software makes operation readily comprehensible. A
context-sensitive online Help function is available for every window and
every dialog.
￭As a means of enhancing safety, no needle movements are carried out
when the door is open.
￭The optional sample cooling guarantees consistent results.
Standard micro titer plates (high or low form) or sample racks can be used.
The same plates or racks must be present in the left-hand and right-hand
loading drawer.
1.1.1 Model versions
2.889.0010 889 IC Sample Center
With:
￭15 µL injection needle
￭500 µL syringe
￭1000 µL buffer loop
￭100 µL sample loop
2.889.0020 889 IC Sample Center Cool
With:
￭15 µL injection needle
￭500 µL syringe
￭1000 µL buffer loop
￭100 µL sample loop
￭Accessories for cooling the samples

1.2 About the documentation ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
2￭￭￭￭￭￭￭￭ 889 IC Sample Center
1.1.2 Instrument components
The 889 IC Sample Center has the following components:
￭2 sample racks
Each for 48 vials.
￭Needle arm
With air needle and drives for horizontal and vertical positioning.
￭Injection valve
With six connectors and two switching positions (LOAD and INJECT)
￭Syringe module
For aspirating and transferring the samples.
￭Cooling module (optional)
For cooling the samples.
￭USB connector
For the connection to a PC.
￭I/O interface
For output of an inject marker signal.
1.1.3 Intended use
The 889 IC Sample Center is designed for usage as an automation system
in analytical laboratories.
This instrument is suitable for processing chemicals and flammable samples.
The usage of the 889 IC Sample Center therefore requires that the user has
basic knowledge and experience in the handling of toxic and caustic sub-
stances. Knowledge with respect to the application of the fire prevention
measures prescribed for laboratories is also mandatory.
1.2 About the documentation
Caution
Please read through this documentation carefully before putting the
instrument into operation. The documentation contains information and
warnings which the user must follow in order to ensure safe operation of
the instrument.
1.2.1 Symbols and conventions
The following symbols and styles are used in this documentation:
Cross-reference to figure legend
The first number refers to the figure number, the sec-
ond to the instrument part in the figure.

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889 IC Sample Center ￭￭￭￭￭￭￭￭ 3
Instruction step
Carry out these steps in the sequence shown.
Warning
This symbol draws attention to a possible life hazard
or risk of injury.
Warning
This symbol draws attention to a possible hazard due
to electrical current.
Warning
This symbol draws attention to a possible hazard due
to heat or hot instrument parts.
Warning
This symbol draws attention to a possible biological
hazard.
Caution
This symbol draws attention to a possible damage of
instruments or instrument parts.
Note
This symbol marks additional information and tips.
1.3 Safety instructions
1.3.1 General notes on safety
Warning
This instrument may only be operated in accordance with the specifica-
tions in this documentation.
This instrument has left the factory in a flawless state in terms of technical
safety. To maintain this state and ensure non-hazardous operation of the
instrument, the following instructions must be observed carefully.
1.3.2 Electrical safety
The electrical safety when working with the instrument is ensured as part of
the international standard IEC 61010.

1.3 Safety instructions ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
4￭￭￭￭￭￭￭￭ 889 IC Sample Center
Warning
Only personnel qualified by Metrohm are authorized to carry out service
work on electronic components.
Warning
Never open the housing of the instrument. The instrument could be dam-
aged by this. There is also a risk of serious injury if live components are
touched.
There are no parts inside the housing which can be serviced or replaced
by the user.
Mains voltage
Warning
An incorrect mains voltage can damage the instrument.
Only operate this instrument with a mains voltage specified for it (see rear
panel of the instrument).
Mains cable
Warning
Replace or repair defective or frayed insulation of the mains cable.
Protection against electrostatic charges
Warning
Electronic components are sensitive to electrostatic charges and can be
destroyed by discharges.
Always pull the mains cable out of the mains connection socket before
connecting or disconnecting electrical appliances on the rear panel of the
instrument.

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889 IC Sample Center ￭￭￭￭￭￭￭￭ 5
Fuses
Warning
Replaced burned fuses only with new fuses of the same size and the same
type, as specified next to the fuse holder or in the accessories list in this
manual.
1.3.3 Tubing and capillary connections
Caution
Leaks in tubing and capillary connections are a safety risk. Tighten all
connections well by hand. Avoid applying excessive force to tubing con-
nections. Damaged tubing ends lead to leakage. Appropriate tools can
be used to loosen connections.
Check the connections regularly for leakage. If the instrument is used
mainly in unattended operation, then weekly inspections are mandatory.
1.3.4 Personnel safety
Warning
Wear protective goggles and working clothes suitable for laboratory work
while operating the 889 IC Sample Center. It is also advisable to wear
gloves when caustic liquids are used or in situations where glass vessels
could break.
Warning
Personnel are not permitted to reach into the working area of the instru-
ment while operations are running!
A considerable risk of injury exists for the user.

1.3 Safety instructions ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
6￭￭￭￭￭￭￭￭ 889 IC Sample Center
Warning
In the event of a possible jamming of a drive, the mains plug must be
pulled out of the socket immediately. Do not attempt to free jammed
sample vessels or other parts while the instrument is switched on. Block-
ages can only be cleared when the instrument is in a voltage-free status;
this action generally involves a considerable risk of injury.
1.3.5 Flammable solvents and chemicals
Warning
All relevant safety measures are to be observed when working with flam-
mable solvents and chemicals.
￭Set up the instrument in a well-ventilated location.
￭Keep all sources of flame far from the workplace.
￭Clean up spilled liquids and solids immediately.
￭Follow the safety instructions of the chemical manufacturer.
￭Dispose of solvents and chemicals according to good professional
practice.
1.3.6 Recycling and disposal
This product is covered by European Directive 2002/96/EC, WEEE – Waste
from Electrical and Electronic Equipment.
The correct disposal of your old equipment will help to prevent negative
effects on the environment and public health.
More details about the disposal of your old equipment can be obtained from
your local authorities, from waste disposal companies or from your local
dealer.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument
889 IC Sample Center ￭￭￭￭￭￭￭￭ 7
2 Overview of the instrument
2.1 Front and rear
1
2
34
Figure 1 889 IC Sample Center - front
1Tubing guide
For capillaries and tubings.
2Door to the sample chamber
With handle.
3Connector for the outlet tubing 4Connector for the condensation and
leakage tubing

2.2 Opening the device ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
8￭￭￭￭￭￭￭￭ 889 IC Sample Center
1
2
3
4
5
Figure 2 889 IC Sample Center - rear
1USB connector
For the connection to a PC.
2Fan
For the cooling unit. Do not cover!
3I/O connector
For output of an inject marker signal.
4Mains connection socket
With mains switch and fuse holder.
5Type plate
Contains specifications concerning mains
voltage and serial number.
2.2 Opening the device
Warning
Mobile parts are to be found in the interior of the 889 IC Sample Cen-
ter. Reaching into the interior during operation puts personnel at a serious
risk of injury. Open the door only if the device is in idle mode. Due to
safety considerations, no needle movements are carried out by the device
when the door is open.

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument
889 IC Sample Center ￭￭￭￭￭￭￭￭ 9
Opening the door
Proceed as follows:
1Grip the door handle:
2Carefully pull out the door and press it upwards until it is in a horizontal
position.
3Slide the door into the housing.

2.3 Interior view ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
10 ￭￭￭￭￭￭￭￭ 889 IC Sample Center
Removing the covering
You can remove the covering on the housing in order to make the interior
more readily accessible:
1Press the two black buttons on the sides of the housing (above) simul-
taneously.
2Carefully pull out the covering towards the front.
2.3 Interior view
Figure 3 Interior view without covering

￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭ 2 Overview of the instrument
889 IC Sample Center ￭￭￭￭￭￭￭￭ 11
2.4 Interior
The following parts are to be found in the interior of the 889 IC Sample
Center:
Figure 4 Interior with sampling device
1Syringe 2Needle arm
3Injection valve 4Sample drip pan
5Sample chamber 6Needle washing position
7Washing bottle

2.5 Cooling option ￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭￭
12 ￭￭￭￭￭￭￭￭ 889 IC Sample Center
2.5 Cooling option
Figure 5 Interior with installed cooling option.
If the cooling option is installed, pull the covering of the cooling option off
towards the front. Now you can place the sample racks or micro titer plates.