Labnet BioPette PLUS User manual

Labnet BioPette Plus
Single-channel, 8-channel,
and 12-channel Pipettors
Instruction Manual
This manual is available in additional languages
at www.labnetlink.com.

Table of Contents
1. Product Description ............................................................... 1
2. Packing.........................................................................................2
3. Pipettor Design.........................................................................2
4. Safety Recommendations .................................................. 4
5. Specications ............................................................................5
6. Pipettor Operation ..................................................................7
7. Operating Instructions ....................................................... 11
8. Aspiration and Dispensing Instructions.......................12
9. Checking Pipetting Accuracy Parameters
and Pipettor Recalibration.................................................14
10. Pipettor Maintenance .........................................................18
11. Troubleshooting ....................................................................19
12. Spare Parts................................................................................21
13. Limited Warranty ..................................................................24

Instruction Manual – 1
1. Product Description
The BioPette Plus single-channel pipettor isavolumetric
instrument designed to measure and transfer liquids
precisely and safely. It is available in volumes from 0.1μL
to 10,000 μL.
The BioPette Plus 8-channel and 12-channel pipettors
are designed for microplate lling. Thepipettors enable
precise and simultaneous delivery of8 or 12 preset
volumes ofliquid. These pipettors are available in four
volume ranges: 0.5-10 μL, 5-50 μL, 20-200 μL, and 50-300 μL.
Nominal Volume
(µL) Cat. No.
Range of Pipettor
Volumes (µL)
Single-channel pipettors
2 P3942-2 0.1 - 2
10 P3942-10 0.5 - 10
20 P3942-20 2 - 20
100 P3942-100 10 - 100
200 P3942-200 20 - 200
1,000 P3942-1000 100 - 1,000
5,000 P3942-5000 1,000 - 5,000
10,000 P3942-10000 1,000 - 10,000
8-channel and 12-channel Pipettors
8-10
12-10
P4808-10
P4812-10 0.5 - 10
8-50
12-50
P4808-50
P4812-50 5 - 50
8-200
12-200
P4808-200
P4812-200 20 - 200
8-300
12-300
P4808-300
P4812-300 50 - 300
Labnet pipettors operate using an air-cushion (i.e.,the
aspirated liquid does not come into contact with the
shaft or plunger of the pipettor). The liquid is drawn into
thedisposable tip attached to the pipettor.

2 – LABNET BIOPETTE PLUS PIPETTORS
2. Packing
The pipettors are delivered with the following:
Description Qty/Pk
Instruction manual 1
Quality Control certicate 1
Calibration key 1
Pipettor hanger 1
Identication labels 1
Lubricant (for single-channel pipettor) 1
Ejector cap (for pipettor models P3942-2,
P3942-10, P3942-5000, P3942-10000) 1
Filters (for pipettor models
P3942-5000, P3942-10000) 3
Filters
The 5,000 μL and 10,000 μL pipettors include areplaceable
lter, tted at the bottom of the shaft which prevents
aspirated liquid from entering the shaft and contaminating
the inner surface and plunger. Use of the lter is particularly
important when aspirating and dispensing large volumes
ofliquid. The lter should be replaced if it becomes wet.
3. Pipettor Design
Model Identication
The volume range of the pipettor is shown on the
pushbutton and on the tip ejector bar in multi-channel
pipettors.

Instruction Manual – 3
Single- and Multi-channel Pipettors
A1
A2
N
B1
B2
C
D
R
A
F
A. Pipetting pushbutton: Consists of 2 parts:
thepushbutton (A1) and the knob (A2).
B1. Volume adjustment knob: Used to set thevolume.
B2. Locking Ring: Allows the volume setting to be locked by
pushing the ring upwards.
C. Shaft: Manufactured using high quality plastic,
ensuring high chemical and mechanical strength.
D. Tip ejector: For multi-channel pipettors, the tips are
ejected sequentially, reducing the required force.
F. Shaft nut
N. Ejector pushbutton
R. Multi-channel manifold: Contains a set of plungers
andaset of exible-suspension shafts, which function
to reduce the force required to attach the tip.

4 – LABNET BIOPETTE PLUS PIPETTORS
4. Safety Recommendations
Long-term use of the pipettor relies on correct method of use.
Please read and follow the instructions for use carefully.
Symbols used:
Danger, risk of injury.
NOTE Risk of damage to the pipettor or errors in pipetting.
NOTE:
• The pipettor is designed for the transfer of liquids only
using the tip. Do not aspirate liquids without the tip
attached. The aspirated liquid should not enter the
pipettor, asit may cause damage.
• Single-use tips reduce the risk of contamination of
samples.
• Keep the pipettor clean, avoiding the use of abrasive or
corrosive cleaning agents (e.g., acetone).
• Keep the pipettor upright when there is liquid in the tip.
• Only using the pipettor in accordance with the
manufacturer’s instructions ensures the correct pipettor
parameters are maintained.
• After replacing the plunger or the shaft, the pipettor
should be calibrated.
• In the case of incorrect operation, the device should
be cleaned in accordance with the Instructions for Use
ortransferred to a service point.
• Ambient operating temperature is +5°C to 45°C.
• Ambient storage conditions (in the original packaging
during transport and short storage) is -25°C to 55°C.
When working with the pipettor:
• Follow general work safety regulations regarding
hazards related to work in the laboratory.
• Take special care when pipetting aggressive substances.
• Use appropriate protective attire (e.g., clothing, goggles,
and gloves).
• Avoid pointing the pipettor at yourself or others during use.
• Only use parts and accessories recommended by the
manufacturer.

Instruction Manual – 5
5. Specications
The pipettor is a high quality instrument which oers
excellent accuracy and precision. The accuracy and precision
(repeatability) of the liquid volume depends on the quality
of pipet tips used. The values for accuracy and precision
shown in the table below were obtained using manufacturer
non-lter pipet tips. Those tips are recommended for use to
ensure compatibility, accuracy and precision when pipetting.
Nominal
Volume
(μL)
Volume
(μL)
Accuracy
(%)
Precision
(%)
Non-lter
Tips (μL)
Single-channel Pipettors
2
Min. 0.1 ±40.0 ≤12.0
10
0.2 ±12.0 ≤6.0
1 ±2.7 ≤1.3
Max. 2 ±1.5 ≤0.7
10
Min. 0.5 ±4.0 ≤2.8
10
1.0 ±2.5 ≤1.8
5.0 ±1.0 ≤0.6
Max. 10.0 ±0.5 ≤0.4
20
Min. 2 ±3.0 ≤1.5
20010 ±1.0 ≤0.5
Max. 20 ±0.8 ≤0.3
100
Min. 10 ±1.6 ≤0.80
20050 ±0.8 ≤0.24
Max. 100 ±0.8 ≤0.20
200
Min. 20 ±1.2 ≤0.60
200100 ±0.8 ≤0.25
Max. 200 ±0.6 ≤0.20
1,000
Min. 100 ±1.6 ≤0.40
1,000500 ±0.7 ≤0.20
Max. 1,000 ±0.6 ≤0.15
5,000
Min. 1,000 ±0.6 ≤0.25
5,0002,500 ±0.6 ≤0.20
Max. 5,000 ±0.5 ≤0.15
10,000
Min. 1,000 ±2.5 ≤0.6
10,0005,000 ±0.8 ≤0.3
Max. 10,000 ±0.5 ≤0.2

6 – LABNET BIOPETTE PLUS PIPETTORS
Nominal
Volume
(μL)
Volume
(μL)
Accuracy
(%)
Precision
(%)
Non-lter
Tips (μL)
8-channel and 12-channel Pipettors
8 - 10
12 - 10
Min. 0.5 ±10.0 ≤8.0
10
1 ±8.0 ≤6.0
5 ±4.0 ≤2.0
Max. 10 ±2.0 ≤1.2
8 - 50
12 - 50
Min. 5 ±4.0 ≤2.5
20025 ±3.0 ≤1.2
Max. 50 ±1.6 ≤0.6
8 - 200
12 - 200
Min. 20 ±3.0 ≤1.5
200100 ±1.5 ≤0.8
Max. 200 ±1.0 ≤0.6
8 - 300
12 - 300
Min. 50 ±1.6 ≤1.5
300150 ±1.2 ≤1.0
Max. 300 ±1.0 ≤0.6
The accuracy and precision were obtained gravimetrically
using manufacturer tips performing at least 10measurements
ofdistilled water at a temperature of 20°C ± 1°C according to
ENISO 8655 standards.
The use of tips from other manufacturers or lter tips may
result in incorrect liquid aspiration and require pipettor
recalibration.
The design of the pipettor enables the user to recalibrate it
according to the information presented in Section 9.

Instruction Manual – 7
6. Pipettor Operation
Attaching the Tips
• Attach the correct tip corresponding to the model number
displayed on the pipettor pushbutton (Section5).
• Position the pipettor vertically when attaching tips.
• Single-channel pipettors: push the pipet tip on rmly
using a slight twisting motion to ensure an airtight seal.
• Multi-channel pipettors: press the pipettor against the
tips positioned in the rack box until the shaft retracts
approximately 1.5mm into the manifold. The suspension
system ensured even and airtight sealing of the pipet tips.
The rocking motion does not have to be performed to seal
the pipet tips tightly.
✓✕ ✓✕
NOTE:
• Do not attach the tips with a rocking movement,
as this may damage the shaft or plunger. Observe this
rule particularly with single-channel pipettors of low
volume range.
• Never draw liquids directly into the pipettor without
thetip attached.
Adjusting the tip ejector
The tip ejector can be adjusted by the user and can
accommodate most types of tip available on the market.
When using narrow tubes, it may be necessary to remove
the tip ejector.

8 – LABNET BIOPETTE PLUS PIPETTORS
2-1,000 μL pipettors
M
M
4
M
M
4
M
M
4
1. Tip ejector disassembly
• Press the tip ejector pushbutton.
• Turn the tip ejector bush 1/3 turn counter-clockwise.
• Slide the ejector from the plastic arbor.
2. Tip ejector assembly
• Press the tip ejector pushbutton.
• Line the tip ejector up with the plastic arbor.
• Turn the tip ejector bush clockwise until it has fully
latched.
3. Tip ejector adjustment
• Remove the tip ejector pushbutton.
• To increase the ejector length turn the plastic bush
counter-clockwise.
• To decrease the ejector length turn the plastic bush
clockwise.