Beckman Coulter Allegra 64R User manual

GS30-IM-11AA
September 2011
Instructions For Use
Allegra 64R
Compact Centrifuge
IMBALANCE
ENTER
PULSE
ACCEL
DECEL
RPM
RCF
ROTOR
TIMETEMP
STOP
START
FAST
STOP
OPEN
DOOR
SPEEDTIMEACC/DEC
TEMP°C
®
Beckman Coulter, Inc.
250 S. Kraemer Blvd.
Brea, CA 92821

Allegra 64R
Compact Centrifuge
GS30-IM-11AA (September 2011)
Copyright © 2011 Beckman Coulter, Inc.
All rights reserved. No part of this document may be
reproduced or transmitted in any form or by any means,
electronic, mechanical, photocopying, recording, or
otherwise, without prior written permission from
Beckman Coulter, Inc.
Find us on the World Wide Web at:
www.beckmancoulter.com
Beckman Coulter do Brasil Com e Imp de Prod de Lab Ltda
Estr dos Romeiros, 220 - Galpao G3 - Km 38.5
06501-001 - Sao Paulo - SP - Brasil
CNPJ: 42.160.812/0001-44
製造販売元 : ベ ッ ク マ ン･コールタ ー株式会社
東京都江東区有明三丁目 5 番 7 号
TOC 有明ウエス ト タ ワー
贝克曼库尔特有限公司，
美国加利福尼亚州，Brea 市，S. Kraemer 大街 250 号，
邮编：92821 电话：[phone]

GS30-IM-11AA iii
Safety Notice
Read all product manuals and consult with Beckman Coulter-trained personnel before attempting
to operate instrument. Do not attempt to perform any procedure before carefully reading all
instructions. Always follow product labeling and manufacturer’s recommendations. If in doubt as
to how to proceed in any situation, contact your Beckman Coulter Representative.
Alerts for Danger, Warning, Caution, and Note
DANGER
DANGER indicates an imminently hazardous situation which, if not avoided,
will result in death or serious injury.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided,
could result in death or serious injury.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided,
may result in minor or moderate injury. It may also be used to alert against unsafe
practices.
NOTE NOTE is used to call attention to notable information that should be followed during installation, use,
or servicing of this equipment.
Safety During Installation and/or Maintenance
This centrifuge weighs 102.1 kg/225 lb. DO NOT attempt to lift or move it without assistance from
another person.
Be sure to use the anchoring system to secure the centrifuge in place. The anchoring system is
designed to reduce the possibility of injury or damage which could result from instrument
movement in the event of a major rotor mishap.
Any servicing of this equipment that requires removal of any covers can expose parts which involve
the risk of electric shock or personal injury. Make sure that the power switch is turned off and the
instrument is disconnected from the main power source, and refer such servicing to qualified
personnel.
Do not replace any centrifuge components with parts not specified for use on this instrument.

GS30-IM-11AA
iv
Safety Notice
Electrical Safety
Electrical Safety
To reduce the risk of electrical shock, this equipment uses a three-wire electrical cord and plug to
connect this equipment to earth-ground. To preserve this safety feature:
• Make sure that the matching wall outlet receptacle is properly wired and earth-grounded.
Check that the line voltage agrees with the voltage listed on the name-rating plate affixed to the
instrument.
• Never use a three-to-two wire plug adapter.
• Never use a two-wire extension cord or a two-wire non-grounding type of multiple-outlet
receptacle strip.
Do not place containers holding liquid on or near the chamber door. If they spill, liquid may get into
the instrument and damage electrical or mechanical components.
Safety Against Risk of Fire
Certain electrical circuits within this equipment are protected by fuses against overcurrent
conditions. For continued protection against the risk of fire, replace only with the same type and
rating specified.
This centrifuge is not designed for use with materials capable of developing flammable or explosive
vapors. Do not centrifuge such materials (e.g., chloroform, ethyl alcohol, etc.) in this instrument nor
handle or store them within the 30-cm (1-ft) clearance “safety envelope” surrounding the
centrifuge.
Mechanical Safety
For safe operation of the equipment, observe the following:
• Use only the Beckman rotors and accessories designed for use in this centrifuge.
• Before starting the centrifuge, make sure that the rotor tie-down screw is securely fastened.
• Do not exceed the maximum rated speed of the rotor in use.
• NEVER attempt to slow or stop the rotor by hand.
• Do not lift or move the centrifuge while the rotor is turning.
• If a glass tube breaks inside the chamber bowl, be careful when examining or cleaning the
gasket or chamber, as sharp glass fragments may be embedded in their surfaces.
• NEVER attempt to override the door interlock system while the rotor is spinning.
• Maintain a 7.6-cm (3-in.) clearance envelope around the centrifuge (secured with antirotation
kit) while it is running. During operation you should come within the envelope only to adjust
instrument controls, if necessary. Never bring any flammable substances within the 30-cm
(1-ft) area surrounding the centrifuge. Never lean on the centrifuge or place items on the
centrifuge while it is operating.

GS30-IM-11AA v
Safety Notice
Chemical and Biological Safety
Chemical and Biological Safety
Normal operation may involve the use of solutions and test samples that are pathogenic, toxic, or
radioactive. Such materials should not be used in this instrument, however, unless all necessary
safety precautions are taken.
• Observe all cautionary information printed on the original solution containers prior to
their use.
• Handle body fluids with care because they can transmit disease. No known test offers complete
assurance that they are free of micro-organisms. Some of the most virulent—Hepatitis (B and
C) and HIV (I–V) viruses, atypical mycobacteria, and certain systemic fungi—further emphasize
the need for aerosol protection. Handle other infectious samples according to good laboratory
procedures and methods to prevent spread of disease. Because spills may generate aerosols,
observe proper safety precautions for aerosol containment. Do not run toxic, pathogenic, or
radioactive materials in this centrifuge without taking appropriate safety precautions. Biosafe
containment should be used when Risk Group II materials (as identified in the World Health
Organization Laboratory Biosafety Manual) are handled; materials of a higher group require
more than one level of protection.
• Dispose of all waste solutions according to appropriate environmental health and safety
guidelines.
It is your responsibility to decontaminate the instrument and accessories before requesting service
by our Field Service representative.

GS30-IM-11AA
vi
Safety Notice
Chemical and Biological Safety

vii
Contents
Safety Notice,iii
Alerts for Danger, Warning, Caution, and Note,iii
Safety During Installation and/or Maintenance,iii
Electrical Safety,iv
Safety Against Risk of Fire,iv
Mechanical Safety,iv
Chemical and Biological Safety,v
Introduction,xiii
Certification,xiii
Scope of this Manual,xiii
Typographic Conventions,xiv
CFC-Free Centrifugation,xiv
Radio Interference,xiv
Recycling Label,xv
Specifications,xvi
Available Rotors,xviii
CHAPTER 1: Description,1-1
Introduction,1-1
Instrument Function and Safety Features,1-1
Instrument Function,1-1
Safety Features,1-2
Name Rating Plate,1-2
Chassis,1-3
Housing,1-3
Door,1-3
Rotor Chamber,1-3
Drive,1-4

viii
Contents
Temperature Sensing and Control,1-4
Controls and Indicators,1-4
Power Switch,1-4
Control Panel,1-5
CHAPTER 2: Installation Requirements,2-1
Introduction,2-1
Preinstallation Requirements,2-1
Space Requirements,2-2
Electrical Requirements,2-3
Test Run,2-4
CHAPTER 3: Operation,3-1
Introduction,3-1
Run Procedure,3-1
Preparation and Loading,3-2
Entering Run Parameters,3-4
Starting a Run,3-8
Changing Parameters During a Run,3-9
Stopping a Run,3-9
Unloading,3-10
Summary of Run Procedures,3-10
CHAPTER 4: Troubleshooting,4-1
Introduction,4-1
User Messages,4-1
Other Possible Problems,4-3
Emergency Access,4-4
CHAPTER 5: Care and Maintenance,5-1
Introduction,5-1
Maintenance,5-1
Preventive Maintenance,5-1
Cleaning,5-2
Tube Breakage,5-3
Decontamination,5-3
Sterilization and Disinfection,5-3
Storage and Transport,5-4
Storage,5-4

ix
Contents
Returning a Centrifuge,5-4
Supply List,5-4
Replacement Parts,5-4
Supplies,5-5
APPENDIX A: Program Library,A-1
Procedure,A-1
Beckman Coulter, Inc.
Allegra 64R, GS-15 Series, Allegra 21 Series, and
Spinchron 15 Series Centrifuge Warranty

x
Illustrations
1.1 The Allegra 64R Centrifuge,1-2
1.2 Interior View of the Rotor Chamber,1-3
1.3 The Power Switch,1-4
1.4 The Control Panel,1-5
2.1 Centrifuge Dimensions,2-2
2.2 North American Electrical Socket,2-3
3.1 Installing a Rotor,3-3
3.2 Default Parameters,3-4
4.1 Emergency Door Release,4-4

xi
Tables
Tables
3.1 Acceleration and Deceleration Rates (in seconds per
1000 rpm),3-7
4.1 Error Message Chart,4-2
4.2 Troubleshooting Chart,4-3

xii
Tables

GS30-IM-11AA xiii
Introduction
Certification
To ensure full system quality, Beckman Coulter Allegra® 64R centrifuges have been manufactured
in a registered ISO 9001 or 13485 facility. They have been designed and tested to be compliant (when
used with Beckman Coulter rotors) with the laboratory equipment requirements of applicable
regulatory agencies. Declarations of conformity and certificates of compliance are available at
www.beckmancoulter.com.
Scope of this Manual
This manual is designed to familiarize you with the Allegra 64R centrifuge, its functions,
specifications, operation, and routine operator care and maintenance.
We recommend that you read this entire manual, especially the Safety Notice and all safety-related
information, before operating the centrifuge or performing instrument maintenance.
• The following introductory pages contain the instrument specifications, as well as space,
electrical, and temperature conditions required for optimal centrifuge performance. A list of
available rotors is also included.
• CHAPTER 1 provides a brief physical and functional description of the centrifuge and the
operating controls and indicators.
• CHAPTER 2 contains instructions for installing and connecting the centrifuge.
• Procedures for operating the centrifuge are summarized in CHAPTER 3.
• CHAPTER 4 lists possible error messages and/or malfunctions, together with probable causes
and corrective actions required.
• Procedures for operator care and maintenance are presented in CHAPTER 5, as well as a brief
list of supplies and replacement parts.
• A Program Library chart is provided at the back of this manual. You can use this chart to record
the parameters of a run for later duplication of the run conditions.
NOTE If the centrifuge is used in a manner other than specified in this manual, the safety and performance
of this equipment could be impaired. Further, the use of any equipment other than that intended for use
by Beckman Coulter has not been evaluated for safety. Use of any equipment not specifically
recommended in this manual is the sole responsibility of the user.

GS30-IM-11AA
xiv
Introduction
Conventions
Conventions
Certain symbols are used in this manual to call out safety-related and other important information.
These international symbols may also be displayed on the centrifuge and are reproduced on the
inside of the back cover.
Typographic Conventions
Certain typographic conventions are used throughout this manual to distinguish names of user
interface components, such as keys and displays.
• Key names (for example, START or ENTER) and display names (for example, TEMP°C or SPEED)
appear in bold type.
• Cursor keys, used to increment values up or down when setting parameters, are shown as up and
down arrows (or ).
CFC-Free Centrifugation
To ensure minimal environmental impact, no CFCs are used in the manufacture or operation of
Allegra 64R centrifuges.
Radio Interference
This instrument has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of FCC Rules. These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used
in accordance with this instruction manual, may cause interference to radio communications.
Operation of this equipment in a residential area may cause interference, in which case the user
will be required to correct the interference at his or her own expense.
Canadian Regulations
This digital apparatus does not exceed the Class A limits for radio noise emissions from digital
apparatus as set out in the radio interference regulations of the Canadian Department of
Communications.
Le présent appareil numérique n’émet pas de bruits radioélectriques dépassant les limites
applicables aux appareils numériques de Classe A prescrites dans le reglement sur le brouillage
radioelectrique édicté par le Ministère des Communications du Canada.

GS30-IM-11AA xv
Introduction
Recycling Label
Recycling Label
This symbol is required in accordance with the Waste Electrical and Electronic Equipment (WEEE)
Directive of the European Union. The presence of this marking on the product indicates.
1. the device was put on the European market after August 13, 2005 and
2. the device is not to be disposed via the municipal waste collection system of any member state
of the European Union.
It is very important that customers understand and follow all laws regarding the proper
decontamination and safe disposal of electrical equipment. For Beckman Coulter products bearing
this label please contact your dealer or local Beckman Coulter office for details on the take back
program that will facilitate the proper collection, treatment, recovery, recycling and safe disposal
of the device.
A28219-AA

GS30-IM-11AA
xvi
Introduction
Specifications
Specifications
Only values with tolerances or limits are guaranteed data. Values without tolerances are informative data,
without guarantee.
Specification Description
Set speed 0 to 30,000 RPM (in 100-RPM increments) or equivalent RCF
Speed display digital display indicates actual rotor speed 50 RPM (actual RCF can be
selected during operation)
Set time to 9 hr 59 min or continuous
Time display time remaining in run (timed run) or and elapsed time (continuous run)
Acceleration ten acceleration profiles
Deceleration ten deceleration profiles
Temperature • Te m p e r a t u r e s e t t i n g
—–20 to +40°C (in 1°C increments)
• Operating range
—2 to 40°Ca
• Ambient temperature range
—10 to 35°C
• Ambient temperature range for optimum operation
—10 to 25°C
Humidity restrictions <95% (noncondensing)

GS30-IM-11AA xvii
Introduction
Specifications
Dimensions • Width
—61 cm (24 in.)
• Depth
—66 cm (26 in.)
• Height, door closed
—38 cm (15 in.)
• Height, door open
—84 cm (33 in.)
Weight 102.1 kg (225 lb)
Clearances (sides and
rear)
7.6 cm (3.0 in.)
Electrical requirements • 50 Hz, 200 VAC, 12 A
• 60 Hz, 200 VAC, 12 A
• 60 Hz, 208 VAC, 12 A
• 50 Hz, 230 VAC, 12 A
Electrical supply Class I
Motor 760 W
Maximum heat dissipation
into room under steady-
state conditions
5400 Btu/h (1.58 kW)
Noise level 0.91 m (3 ft) in
front of instrument
65 dBa
Installation (overvoltage)
category
II
Pollution degree 2b
a. Temperature range depends on rotor in use and speed (see applicable rotor manual).
b. Normally only nonconductive pollution occurs; occasionally, however, a temporary conductivity caused by condensation
must be expected.
Specification Description

GS30-IM-11AA
xviii
Introduction
Available Rotors
Available Rotors
Rotor Profile Description
Max
RPM
Max
RCFa
(g)
Max RPM
at 4°Cb
Max
RCF*
(g)
at 4°Cb
Max
Capacity
(mL)
Rotor Part
Number/
Rotor Manual
Number
F1202
Fixed Angle
45° Angle
30,000 64,400 28,500 58,120 12 2.0/1.5 364630
GS30-TB-006
F2402H
Fixed Angle
45° Angle
26,000c61,970 21,000 40,430 24 2.0/1.5 361171
GS-TB-021
F3602
Fixed Angle
45° Angle
22,000 47,620/
41,670
19,500 37,410/
32,700
36 2.0/1.5 364600
GS-TB-006
F0630
Fixed Angle
30° Angle
26,200c59,860 21,500 40,310 6 38.5 361231
GS-TB-014
F0650
Fixed Angle
25° Angle
21,000 41,420 18,500 32,140 6 50 364610
GS30-TB-004
F0850
Fixed Angle
25° Angle
16,500 29,220 16,500 29,220 8 50 364640
GS-TB-003
F0485
Fixed Angle
30° Angle
20,000 40,700 17,500 31,160 4 85 364620
GS30-TB-007
F0685
Fixed Angle
25° Angle
15,500 26,320 15,000 24,650 6 85 364650
GS-TB-008
F1010
Fixed Angle
35° Angle
26,000c57,440 22,500 43,020 10 10 361221
GS-TB-007
C0650
(Conical)
Fixed Angle
25° Angle
10,000 10,400 10,000 10,400 6 50 364670
GS-TB-009
C1015
(Conical)
Fixed Angle
25° Angle
10,000 10,400 10,000 10,400 10 15 364680
GS-TB-011

GS30-IM-11AA xix
Introduction
Available Rotors
S0410
Swinging
Bucket
Four
buckets
10,000 10,730 10,000 10 730 4 10 364660
GS30-TB-005
H6002
BioSafe
Bowl Rotor
12,200 12,400 12,200 12 400 60 1.5/1.8
72 600 L
84 250 L
363000
GS30-TB-002
a. Values have been rounded off.
b. Measured at ambient temperature of 21°C.
c. Reduce maximum speed to maintain temperature control for runs exceeding 10 minutes.
Rotor Profile Description
Max
RPM
Max
RCFa
(g)
Max RPM
at 4°Cb
Max
RCF*
(g)
at 4°Cb
Max
Capacity
(mL)
Rotor Part
Number/
Rotor Manual
Number

GS30-IM-11AA
xx
Introduction
Available Rotors