Microtek Bio-5000 User manual

Getting to KnowYour Bio-5000

The Bio-5000 is a combined flatbed and film scanner for electrophoresis gels and film

formats that also offers additional functionality to scan reflective art or prints as large

as 8.5" x 14". The Bio-5000 is equipped with Microtek's patented Emulsion Direct

Imaging Technology (E.D.I.T.) for delivering distortion-free images in transparency

scanning. The 0.05 minimum optical density (Dmin) provides a wider and clearer range

for capturing light tones of images.

I49-004655 E, September 2013

Bio-5000 features, scenarios, and information

Supplement

Contents

Getting to KnowYour Bio-5000 ....................................................................... 1

Features of the Bio-5000 .................................................................................. 2

Taking a Closer Look ........................................................................................ 3

Unlocking the Scanner .................................................................................... 4

PositioningYour Originals .............................................................................. 5

Positioning Electrophoresis Gels ........................................................... 6

Using the Leak-Free Glass Holder .................................................. 6

PositioningTransparent Film ................................................................. 7

Using the Leak-Free Glass Holder .................................................. 7

Positioning Reflective Originals ............................................................. 8

Scanning Scenarios ......................................................................................... 9

Scanning Electrophoresis Gels ............................................................ 10

ScanningTransparent Film ................................................................... 12

Scanning Reflective Originals ............................................................... 14

Scanning Originals Applied with Auto Focus ..................................... 16

Specifications / System Requirements ........................................................ 18

FCC Compliance Statement .......................................................................... 19

2 Bio-5000 Supplement

The Bio-5000 comes with several important features, including the following:

• One machine to scan all: With specially designed Leak-Free Glass Holders, the

Bio-5000 allows you to scan electrophoresis gels and transparent films directly in

the machine. It also supports scanning of reflective materials, such as photos and

prints.

• Microtek's Emulsion Direct Imaging Technology (E.D.I.T.): This is a patented

“glassless” scanning system built into the lower bay of Microtek’s dual media

scanners, allowing the scanner CCD to directly read the emulsion side of the film

during scanning without any interfering pane of glass. This effectively eliminates

problems associated with normal glass transparency scanning like Newton Rings,

resulting in distortion-free images.

• Auto Focus:An image improvement feature introduced to Microtek flatbeds,Auto

Focus allows the scanner to change the focus position on images through the

movement of the CCD, resulting in better image quality for a chosen area of the

scan. Auto Focus is activated by default when the scanner is on but can be

switched off manually before the final scan is carried out. The results of Auto

Focus can be best seen when used with uneven, creased photos and film.

• 4800 x 9600-dpi resolution: The exceptionally high resolution of the Bio-5000 lets

you scan even postage-size images and enlarge them with amazing clarity, with

little loss of detail. The scanner's 0.05 minimum optical density (Dmin) allows it to

capture a wide range of dark tones approximating real-life color and hues.

• Microtek ScanWizard™ Bio scanning software: This is an advanced scanner

controller program that provides many powerful, professional-level features for

scanning.

• Hi-SpeedUSB interface: High-Speed USB(USB2.0) , which iscapableof 480Mbps

data transfer, provides plug-and-play connectivity for a hassle-free installation.

Features of the Bio-5000

Bio-5000 Supplement 3

Taking a Closer Look

Horizontal ruler

Scanner lid

Glass surface

(Upper scan bed)

Vertical ruler

Power button

Power indicator (green)

Reflective Ready

indicator (amber)

Transparency Ready

indicator (amber)

Power connector Hi-Speed USB port (1)

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Unlocking the Scanner

Before you can operate the scanner, you will need to unlock the scanner. To unlock

the scanner, follow the steps below:

1. Remove the yellow “Unlock” sticker

from your scanner.

2. Look for the unlocking screw at the

bottom of the scanner.

3. Using a screwdriver, push and turn

the locking screw counterclockwise

to the unlock position.

When successfully unlocked, the

screw will push out a little,

protruding slightly from the bottom

of the scanner.

Shipping the Scanner

If you have to transport the scanner, you will need to lock the scanner back. Follow

the steps below:

1. Turn off the scanner if your scanner

is on.

2. Turn the scanner back on. The

scanner’s carriage will move to the

standby position in 5 minutes.

3. When the indicators on the front of

your scanner stop blinking, use a

screwdriver, and then push and turn

the locking screw clockwise to the

locked position.

When the screw has been tightened, this indicates that your scanner is locked.

4. Turn off your scanner. The scanner is now ready for transport.

Bio-5000 Supplement 5

PositioningYour Originals

Aside from the scanning of reflectives, there are two pieces of Leak-Free Glass

Holders come with your scanner package, which are specifically designed to scan

electrophoresis gels and transparent film.

Before proceeding your scan, take note of the information presented below:

LEAK-FREE GLASS HOLDER

This holder is specifically designed for 

the use of the gel and transparent film scanning.

Leak-Free Glass Holders (2 pieces)

(used to scan electrophoresis gels and

transparent film)

Proper positioning of the scan material on the scanner is important for successful

scanning. Before starting scanning, refer to the individual section in the succeeding

pages about how to correctly position the electrophoresis gels, transparent film, and

reflectives.

Calibration strip

LEAK-FREE GLASS HOLDER

Thisholder is specifically designed for 

theuse of the gel and transparent film scanning.

The correct side up is the

side showing the holder

labels facing up

• When using the Leak-Free Glass Holders, make sure you use

the correct side up when inserting the holders into the scanner.

The correct side up is the side showing the holder labels facing

up.

• Make sure that the calibration strip on the holders are kept clear

and free of obstruction at all times; no material should ever be

placed on this area. Also, make sure the calibration strip faces the

front of the scanner when you insert the holder into the lower

compartment of the Bio-5000.

• Please take note that the high temperature may cause the damage

to your electrophoresis gels, transparencies and reflective

materials. Therefore, remember to turn off the scanner and remove

the scanned materials from the scanner once you finish the scan

job.

LEAK-FREE GLASS HOLDER

This holder is specifically designed for 

the use of the gel and transparent film scanning.

Note: The Leak-Free Glass Holders require regular cleaning. To clean, use mild glass cleaning solution, and

wipe the glass plate gently with lint-free, lens-cleaning cloth to prevent leaving fiber residue.

6 Bio-5000 Supplement

Positioning Electrophoresis Gels

This procedure applies when you use the lower scan bed of the Bio-5000 to scan the

electrophoresis gels.

Using the Leak-Free Glass Holder

1. Place the electrophoresis gel to be scanned on top of the glass surface of the

Leak-FreeGlassHolder.

LEAK-FREE GLASS HOLDER

This holder is specifically designed for 

the use of the gel and transparent film scanning.

Keep calibration strip clear

and free of any obstruction

2. Insert the holder all the way into the transparency bay (lower compartment) of the

scanner. Make sure the holder is inserted correct side up.

LEAK-FREE GLASS HOLDER

Thisholder is specifically designed for 

theuse of the gel and transparent film scanning.

Bio-5000 Supplement 7

Positioning Transparent Film

This procedure applies when you use the lower scan bed of the Bio-5000 to scan

transparencies, such as positive or negative film.

Using the Leak-Free Glass Holder

1. Place the film to be scanned face down on top of the glass surface of the Leak-

FreeGlass Holder.

2. Secure the film to the glass by using the vinyl strips come with your scanner

package.

LEAK-FREE GLASS HOLDER

This holder is specifically designed for 

the use of the gel and transparent film scanning.

Keep calibration strip clear

and free of any obstruction

3. Insert the holder all the way into the transparency bay (lower compartment) of the

scanner. Make sure that the holder is inserted correct side up.

LEAK-FREE GLASS HOLDER

This holder is specifically designed for 

the use of the gel and transparent film scanning.

Vinyl strip

LEAK-FREE GLASS HOLDER

Thisholder is specifically designed for 

theuse of the gel and transparent film scanning.

8 Bio-5000 Supplement

Positioning Reflective Originals

This procedure applies when you use the upper scan bed of the Bio-5000 to scan

reflective materials, such as photos and prints.

1. Open the scanner lid.

2. Place the original to be scanned face down on the scanner glass bed, towards

the front of the scanner. Center the top of the original along the horizontal ruler

on the scanner.

Note: To scan a book or thick materials/documents, lift the scanner lid out of its

hinge sockets high enough to create room between the originals and the lid.

3. Lower the scanner lid.

Bio-5000 Supplement 9

Scanning Scenarios

The following pages provide various scenarios for scanning with the Bio-5000,

including the following:

• Scanning electrophoresis gels: This scenario details the steps for scanning

electrophoresis gels.

• Scanning transparent film: This scenario details the steps for scanning

transparent film, such as 4" x 5" positive or negative film.

• Scanning reflective originals: This scenario details the steps for scanning

reflective materials, such as photos and prints.

• Scanning originals applied with Auto Focus: This scenario details the steps for

applying the Auto Focus function to a selected area of the image to be scanned,

in order to improve that area of the image.

10 Bio-5000 Supplement

Scanning Electrophoresis Gels

1. Insert the Leak-FreeGlassHolder with the

electrophoresis gel into the transparency bay

of the scanner. Make sure that nothing is on

the scanner glass surface.

2. Launch ScanWizard Bio either as a stand-

alone by clicking on the program icon, or by

using the File-Import or File-Acquire

commandfrom your image-editing program.

3. Go to the Preview window and choose PositiveFilmfrom the ScanMaterial

menu.

4. Click the Overviewbutton to perform a preliminary scan of your original.

5. Select the Scan Frame tool from the Toolbar in the Preview window, and choose

the area to be scanned by dragging a rectangle around it. You will see a flashing

frame (marquee) around the selected area.

6. Click the Prescan button to display a detailed image area selected via the

Scan Frame tool.Athumbnail of the image appears as well in the Scan Job

Queue window.

7. Specify your scanning requirements in the Settings window.

a) Select a desired image type.

b) Select a desired resolution.

c) Adjust the scan frame settings if necessary.

8. Adjust image quality if necessary, using theAdvanced Image Correction (AIC)

tools.

9. Click the Scan (or “Scan to”) button in the Preview window to start scanning.

• If ScanWizard Bio was launched from an application program, the image is then

delivered to your application, where the image can be saved, printed, or edited.

• If ScanWizard Bio was launched in stand-alone mode, you will be prompted to

specify the file attributes for the scanned image after the Scan or Scan to

button is pressed, such as entering the file name, specifying a folder name of

your own, etc. When you have completed the settings, press the Done/Save

button, and the scanner will automatically scan and save your image based on

your settings.

LEAK-FREE GLASS HOLDER

Thisholder is specifically designed for 

theuse of the gel and transparent film scanning.

Bio-5000 Supplement 11

12 Bio-5000 Supplement

LEAK-FREE GLASS HOLDER

This holder is specifically designed for 

the use of the gel and transparent film scanning.

Scanning Transparent Film

1. Insert the Leak-FreeGlassHolder with the

film into the transparency bay of the

scanner.

Make sure that nothing is on the scanner

glass surface.

2. Launch ScanWizard Bio either as a stand-

alone by clicking on the program icon, or by

using the File-Import or File-Acquire

commandfrom your image-editing program.

3. Go to the Preview window of ScanWizard Bio, and choose the correct type for the

filmfromtheScanMaterialmenu.

Choose Negative Film to scan negatives, or choose Positive Film to scan

positives.

4. Click the Overviewbutton to perform a preliminary scan of your original.

5. Select the Scan Frame tool from the Toolbar in the Preview window, and choose

the area to be scanned by dragging a rectangle around it. You will see a flashing

frame (marquee) around the selected area.

6. Click the Prescan button to display a detailed image.Athumbnail of the image

appears as well in the Scan Job Queue window.

7. Specify your scanning requirements in the Settings window.

a) Select a desired image type.

b) Select a desired resolution.

c) Adjust the scan frame settings if necessary.

8. Adjust image quality if necessary, using the Advanced Image Correction

(AIC) tools.

9. If the colors in your film are faded and need restoring, check the “Automatic Color

Restoration” box in the Settings window.

10. Click the Scan (or “Scan to”) button in the Preview window to start scanning.

• If ScanWizard Bio was launched from an application program, the image is then

delivered to your application, where the image can be saved, printed, or edited.

• If ScanWizard Bio was launched in stand-alone mode, you will be prompted to

specify the file attributes for the scanned image after the Scan or Scan to

button is pressed, such as entering the file name, specifying a folder name of

your own, etc. When you have completed the settings, press the Done/Save

button, and the scanner will automatically scan and save your image based on

your settings.

Bio-5000 Supplement 13

14 Bio-5000 Supplement

Scanning Reflective Originals

1. Raise the scanner lid, and place the

reflective original to be scanned face down

on the scanner glass bed, towards the

front of the scanner. Center the top of the

original along the horizontal ruler on the

scanner.

2. Launch ScanWizard Bio either as a stand-

alone by clicking on the program icon, or

by using the File-Import or File-Acquire command from your image-editing

program.

3. Go to the Preview window of ScanWizard Bio, and choose Reflective from the

ScanMaterialmenu.

4. Click the Overviewbuttonto perform a preliminaryscanof the image, whichwill

appear in the Preview window.

5. Select the Scan Frame tool from the Toolbar in the Preview window, and choose

the area to be scanned by dragging a rectangle around it. You will see a flashing

frame (marquee) around the selected area.

6. Click the Prescan button to display a detailed image area selected via the

Scan Frame tool.Athumbnail of the image appears as well in the Scan Job

Queue window.

7. Specify your scanning requirements in the Settings window.

a) Select a desired image type.

b) Select a desired resolution.

c) Adjust the scan frame settings if necessary.

8. Adjust image quality if necessary, using theAdvanced Image Correction (AIC) tools.

9. If the colors in your reflective original are faded and need restoring, check the

“Automatic Color Restoration” box in the Settings window.