MRC UT-FLU5B User manual

UT-FLU5B
FLUOROMETER
OPERATION MANUAL
3, Hagavish st. Israel 58817 Tel: [phone], Fax: [phone]
[email protected]
MRC. 12.22

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II
Preface
Thank you for purchasing this product. This user manual contains the
functions and operation procedures of the instrument. In order to ensure the
correct use of the instrument, please read the manual carefully before
operating the instrument. Please keep the manual in a safe place so that you
can read it quickly if you encounter a problem.
Unpacking Inspection
When the user unpacks the instrument for the first time, please check
the instrument and accessories against the packing list. If the instrument or
accessories are found to be wrong, incomplete or abnormal, please contact
the seller or manufacturer.

III
Matters Needing Attention
1. Important Safety Operating Information
The user needs to have a complete understanding of how the instrument works
before operating the instrument safely. Users should read this manual carefully before
operating the instrument.
2.Safety
The following basic safety precautions must be observed during all operations,
maintenance, and repair of this instrument.Failure to observe these precautions or the
warnings noted elsewhere in this manual may affect the protection provided by the
instrument and the intended range of use of the instrument.
It is forbidden for anyone to operate the instrument without reading the
manual. If you do not follow the instructions in the manual, the
instrument may cause accidental injury during operation, and electric
shock may occur. Please read the following safety tips and guidance
carefully and implement all precautions contained therein.
This instrument is an ordinary device that complies with the GB4793.1
standard, and this instrument is the product for indoor use.
Please read this manual carefully before operating the instrument,
otherwise personal injury may be caused.Only qualified inspectors trained
in the installation and use of electrical equipment should operate this
instrument.
Except for the parts that can be opened by the user as specified in the
manual, it is strictly forbidden for the user to disassemble the instrument.
Failure to do so will void your warranty and may also expose you to electric
shock. If repair is required, the company is responsible for the repair.
This instrument is only used for scientific research experiments and is
not suitable for clinical testing!
CAUTION: Biological contamination. All test samples, quality controls,
calibrators, etc., should be considered infectious, and gloves should be worn
when contacting; parts that have been in contact with test samples should be
considered infectious, and gloves should be worn when touching;

IV
The instrument should be placed in a dry, dust-free place away from water
and direct sunlight and strong light sources. The room should be well
ventilated, free from corrosive gases or strong magnetic fields, and away
from heaters, stoves and all other heat sources. Do not place the instrument
in a humid or dusty place.
Nothing should be covered by the power cord when the instrument is in
use. Do not place the power cord where people move.
Be sure to hold the plug when plugging and unplugging the power cord.
Make sure that the plug is fully inserted into the socket when plugging in,
and do not pull the power cord hard when unplugging.
Turn off the power when it stops working. When the instrument is not used
for a long time, unplug the power plug and cover the instrument with a soft
cloth or plastic paper to prevent dust from entering.
In the following cases, the power plug of the instrument should be unplugged
from the power outlet immediately, and contact the supplier or a trained
service person to deal with it:Liquid spilled into the instrument;
The instrument is drenched or watered;
The instrument falls or the shell is damaged;
The function of the instrument has changed significantly.

V
Warranty Description
a) Warranty content
Within 1 month from the date of delivery of the instrument, the company will be
responsible for the replacement of the failure caused by defects in materials and
manufacturing.
The instrument is warranted against failures due to defects in material and
manufacture for a period of 12 months from the date of delivery. During the warranty
period, the company will selectively repair or replace instruments that prove to be
defective.
Warranted products must be sent by the user to the maintenance department
determined by the company. The shipping cost of the instrument from the user to the
maintenance department shall be paid by the user. The company bears the shipping cost of
returning the instrument to the user.
For repairs outside the warranty period, the company will properly charge the cost
of repairs.
b) Warranty range
The above warranty does not apply to damage caused by improper use and
maintenance by the user, use in non-compliant conditions, unauthorized repair or
modification.

─1─
Chapter Ⅰ Brief introduction
Fluorescence immunoassay technology has the advantages of strong specificity, high
sensitivity, and good practicability, so it is used to measure biologically active compounds
with very low content,such as proteins (enzymes, receptors, antibodies), nucleic acids,
hormones (steroids, thyroid hormones, phthalohormones), drugs and microorganisms.
This fluorometer is based on fluorescence immunoassay technology, which detects
the luminescence intensity of fluorescent reagents in immunoassays; Under the condition
of low concentration, the concentration of the sample and the fluorescence intensity have
a linear relationship, so the qualitative and quantitative analysis of the detected object is
carried out.

─2─
Chapter Ⅱ Features
Basic parameters and performanc
Normal working conditions
Ambient temperature: 4C 45C
Relative humidity: ≤70%
Power supply: DC 9V 1.5A
Battery:18650 Rechargeable lithium battery(3400mah*2，can last for 5 hours)
e
Specification:
Product name Light source Excitation
wavelength
Emission
wavelength
UT-FLU5B IFluorimeter Blue LED 460±20nm 525nm
Red LED 625±20nm 690nm
UT-FLU5B II Fluorimeter Blue LED 460±20nm 525nm
UV LED 365±20nm 470nm
UT-FLU5B III Fluorimeter Blue LED 460±20nm 525nm
Green LED 525±20nm 620nm
Else customized
Model
Parameters UT-FLU5B
light source Monochrome LED
Linearity 2
R>0.995
Repeatability <1.5%
Stability <1.5%
Sensitivity Ds DNA：1ng/ml；
Measuring speed 3s（single time）
Dimensions
(WXDXH) 194*155*72.5
Net weight (kg) 1.0Kg

─3─
Chapter Ⅲ Instrument composition
This chapter mainly introduces the structure of the instrument and the preparations before
it is turned on. When using the instrument for the first time, you should familiarize yourself
with the contents of this chapter before turning it on.
Structure diagram:
Figure 1 Instrument structure
USB export
Printer interface
USB port
Power socket
Switch
Display
Sample
cover

─4─
Chapter Ⅳ Instrument installation
1. Unpacking inspection
All fluorometers have been strictly inspected by the company before they are packaged
and shipped. When you receive the instrument, please check carefully before unpacking, and
pay attention to the following damages:
①The outer packaging is inverted or deformed;
②The outer package contains obvious traces of wet water;
③The outer package contains obvious signs of impact;
④signs that the outer packaging has been opened;
Once the above damage is found, please contact the company or notify the local agent
immediately.
If the outer package is in good condition, please open the package and check after
unpacking in the presence of agents and staff:
①According to the packing list, check whether all components are complete;
②Carefully check the appearance of all devices to see if there are cracks, bumps or
deformations.
2.Installation requirements
①Space: a dry, clean, level work surface.
②Working environment
a. The ambient air is clean and free of corrosive steam and smoke.
b. Ambient temperature between +10°C and +40°C.
3.Installation steps
①Place the instrument and packaging lightly on the operating site, open the carton, and then
take out the instrument and place it on the operating table.
②Put the "ON/OFF" switch on the back of the instrument to the "OFF" position. Take out the
15V power adapter, insert its plug into the socket at the rear of the instrument, and connect the
other end to the AC100~220V power supply.

─
5─
③Turn on the switch on the back of the instrument, the instrument runs self-test, and can be
tested after completion.
4. Precautions for instrument operation
This fluorometer can use cuvettes, 0.2mL fluorescent PCR tubes, and 0.5mL fluorescent
PCR tubes for sample detection. Corresponding tube holder should be configured when using
fluorescent PCR tube for detection.
Sample testing steps:
1. As shown in the figure below (Figure 3), insert the PCR tube holder (1) first—it has
been inserted in the general instrument;
2. Then insert the PCR tube with the fluorescent sample into the PCR tube holder;
3.Cover the sample cover and click "detecting" in the interface to perform sample
detection
Figure 2 Sample placement
In order to ensure the detection effect of the instrument, when using a 0.2mL fluorescent
PCR tube, the sample volume is 0.1mL; when using a 0.5mL fluorescent PCR tube, the
sample volume is 0.2mL; if the sample volume is not enough, the corresponding solvent can
be used to dilute the sample before testing. .
But be careful when diluting, if the concentration is too low, it will affect the stability of
sample detection. And the amount of solution in the tube when the sample is detected should
be the same as the volume established by the standard curve.
Fluorescent PCR tube
PCR tube socket

─6─
Chapter Ⅴ Software operation
Ⅰ Instrument self-test
Connect the power supply, put down the upper base, press the power switch on the back
of the instrument, the instrument starts and enters the self-test interface:
Figure 3 Boot interface
After the self-check is completed, enter the main interface of the instrument. The
following sections describe each interface in detail.
Ⅱ Main menu
After the self-test is completed, the main interface of the software entered is as shown in
the figure below:
Figure 4 Main menu

─7─
1. Overview
The 6 colored function icons in the main interface are as follows:
：Fluorescence detection;
：dsDNA detection；
：RNA detection；
：Protein detection；
：Oligo detection；
：other detection；
Among them, the "Fluorescence Detection" icon, click to enter, you can select the
fluorescence channel configured by the instrument, and then directly detect the fluorescence
value of the sample.Only fluorescence can be detected here, and there is no curve building
and sample concentration analysis.
The other 5 icons, after entering, can inject standard curve establishment, calibration
(curve), and sample detection. The specific functions of these five icons are exactly the same,
and the five icons are divided into five icons to facilitate classification management when
users use different samples and fluorescent reagents for detection.
2.dsDNA double-stranded DNA detection
Note: Since the software functions of dsDNA, RNA, Protein, Oligo, and Custom are
completely the same, only the software functions of dsDNA detection are described in detail
in this operation manual, and the operations of the other four types of detection refer to

─8─
dsDNA detection.
2.1 dsDNA detection
Click the dsDNA icon to enter the following interface (Figure 5)
Figure 5 Sample detection
In Figure 5:
Standard curve: Select the established standard curve, and the curve information will be
displayed in the lower left corner.
Sample detection: After entering, the fluorescence value of the fluorescent sample can be
detected, and the sample concentration can be calculated according to the selected standard
curve.
Note:
a. When the 0.5mL sample tube is used for detection by the fluorometer, the volume of
the sample to be detected is 200uL. When the sample volume added during the detection is
less than 200uL, it needs to be supplemented to 200uL by the diluent. The concentration of
the sample detected at this time is the concentration of the sample in the tube, while The
instrument calculates the original sample concentration (that is, the actual concentration of the
added test sample) from the added sample volume and the diluent volume.
Actual sample volume
added
Standard curve
selection

─9─
b. If there is no standard curve, this item is unavailable. If you need to directly detect the
fluorescence intensity of the sample, please enter the fluorescence detection interface.
2.2 Standard curve
Create a standard curve
After entering, a standard curve is established through the standard sample, which is used
as the basis for the detection of sample concentration.
①Curve name: You can create a new curve, select an existing curve to view, etc. When
there is no curve in the new instrument, the display here is blank. Click the blank area to pop
up a drop-down menu, in which "+NEW" is the button for creating a new curve. When there
are multiple curves, you can also click the curve name area to pop up all the curves, and you
can select the desired curve. As shown in Figure 6:
Figure 6 Standard curve establishment
①Curve calibration: After entering, the established standard curve can be calibrated to
eliminate the error caused by instrument drift.
②Delete curve: delete unnecessary curve, please operate with caution if it cannot be
Standard sample
selection area
Standard sample
concentration input
area
New standard curve
name
Standard curve
fitting mode
Channel selection

─10 ─
retrieved after deletion.
③Save the curve: After the standard curve data detection is completed, click Save Curve
to save the curve.
④Fitting curve: After the standard curve data detection is completed, you can view the
fitting curve to see the effect of standard curve establishment.
⑤Sample detection: After selecting a standard sample, the fluorescence value of the
standard sample can be detected. As shown in Sample 01 above.
⑥Delete data: Select a single detected fluorescence value and delete it to remove
unnecessary or mishandled fluorescence values.
Methods for establishing a standard curve:
a. Prepare a series of standard samples, 5-9 standard samples;
b. Create a new standard curve and enter the standard curve name;
c. Select the fluorescence detection channel according to the sample type;
d. Enter the standard sample concentration into the table in Figure 6;
e. Detect the fluorescence value of the standard sample separately, pay attention to the
corresponding concentration and fluorescence value;
f. After the detection is completed, click the fitted curve to view the curve effect;
g. Save the standard curve, and then call the standard curve detection position sample in
the detection interface of Figure 5.

─11 ─
Figure 7 Curve fitting
Click "Curve Data" to return to the standard curve interface.
Curve calibration
The curve calibration function is to use a small number of samples (1-3 samples) to
calibrate the curve under the condition of instrument drift, which can save the sample and
operation time for establishing the standard curve while improving the sample detection
accuracy. The "calibration curve" detection sample operation method is basically the same as
the operation method when the curve is established.
Figure 8. Curve calibration
Sample selection area
Concentration input area

─12 ─
Note: The calibration curve can be calibrated with 1-3 sample points. The
principle of calibration point selection:
①Single-point calibration, with blank solution for calibration.
②Two-point calibration, using two high and low concentration sample points that
can basically cover the concentration range of the entire curve.
③Three-point calibration: Two high, medium and low concentration sample points
that can basically cover the concentration range of the entire curve are used, and it is
better that the three sample points are evenly distributed.
Under normal circumstances, two-point calibration can be selected.
2.3 Data record
Click "Data Record" to enter the following interface (Figure 8):
Figure 9. Data record
The data recording interface saves all test data, which can be viewed, deleted, exported,
printed and other operations.

─13 ─
3. Fluorescence detection
Click the "Fluorescence Detection" icon to enter the following interface:
Figure 10. Channel selection
In this interface, the fluorescence channel configured by the instrument can be selected
for sample detection. In the figure, the instrument is configured with two channels of 460nm
and 625nm.
Select the desired fluorescence detection channel (click the corresponding icon) to enter
the next level interface.
Note: The instrument is configured with 2 channels, so the channel needs to be selected
during detection. The instrument configuration channels vary according to the different
subdivision models of the fluorometer. For details, see Chapter 2 Features and Model
Specifications. The selected detection channel should correspond to the excitation and
emission wavelengths of the fluorescent reagents used, otherwise correct results cannot be
obtained.
When you click Blue (460nm) in Figure 6, you will enter the fluorescence detection
interface as shown below:

─14 ─
Figure 11 Fluorescence detection
The interface can detect samples, print data, etc.
Note: Printing requires an external printer to be connected.
Figure 12. Data record
The data recording interface saves all test data, which can be viewed, deleted,
exported, printed and other operations.