MRC SPECTRO-UV600 Series User manual

SPECTRO-UV600 SERIES User’s Guide
I
User’s Guide
SPECTRO-UV600 Series
UV-VIS Spectrophotometer
PLEASE READ THIS MANUAL CAREFULLY BEFORE OPERATION
11.6.23
3, Hagavish st. Israel 58817 Tel: [phone], Fax: [phone]
[e-mail]

SPECTRO-UV600 SERIES User’s Guide
II
Guaranty and Declaration
Copyright
© 2014 All Rights Reserved.
Publication Number
435119
Notices
Our products are protected by patent law in and outside of P.R.C.
We reserves the right to modify or change parts of or all the specifications and
pricing policies at company’s sole decision.
Information in this publication replaces all previously corresponding material.
We shall not be liable for losses caused by either incidental or consequential in
connection with the furnishing, use or performance of this manual as well as
any information contained.
Any part of this document is forbidden to be copied or photocopied or
rearranged without prior written approval of us.
Product Certification
We guarantees this product conforms to the national and industrial standards in
China as well as the ISO9001:2008 standard. Other international standard
conformance certification is in progress.
Contact Us
If you have any problem or requirement when using our products or this manual,
please contact us.

SPECTRO-UV600 SERIES User’s Guide
III
Safety Requirement
General Safety Summary
Please review the following safety precautions carefully before putting the instrument
into operation so as to avoid any personal injuries or damages to the instrument and
any product connected to it. To prevent potential hazards, please use the instrument
only specified by this manual.
Use Proper Power Cord.
Only the power cord designed for the instrument and authorized by local country could
be used.
Ground The Instrument.
The instrument is grounded through the Protective Earth lead of the power cord. To
avoid electric shock, it is essential to connect the earth terminal of power cord to the
Protective Earth terminal before any inputs or outputs.
Observe All Terminal Ratings.
To avoid fire or shock hazard, observe all ratings and markers on the instrument and
check your manual for more information about ratings before connecting.
Do not Assemble/Disassemble the Instrument without Authorization
The instrument should be assembled and or disassembled by Our field engineers,
users are not allowed to perform related operations except for some possible
replacements in this manual.
Do not Move or Maintain the Instrument When it is Powered On
To avoid personnel injuries, any position adjustment, maintenance or parts
replacement are forbidden when instrument is on.
Use Proper Fuse.
Please use the specified fuses.
Do Not Operate With Suspected Failures.
If you suspect damage occurs to the instrument, have it inspected by qualified service
personnel before further operations. Any maintenance, adjustment or replacement
especially to circuits or accessories must be performed by our authorized personnel.
Keep Well Ventilation.
Inadequate ventilation may cause increasing of temperature or damages to the

SPECTRO-UV600 SERIES User’s Guide
IV
device. So please keep well ventilated and inspect the intake and fan regularly.
Do Not Operate in Wet Conditions.
In order to avoid short circuiting to the interior of the device or electric shock, please
do not operate in a humid environment.
Do Not Operate in an Explosive Atmosphere.
In order to avoid damages to the device or personal injuries, it is important to
operate the device away from an explosive atmosphere.
Keep Product Surfaces Clean and Dry.
To avoid the influence of dust and/or moisture in air, please keep the surface of device
clean and dry.
Operate the Instrument in a Suitable Environment
The best condition for optimal measurement is: 5℃to 35℃; less than 2℃/hour
changing, 20% to 80% RH and the instrument should not be placed towards the air
outlet.
Electrostatic Prevention.
Operate in an electrostatic discharge protective area environment to avoid damages
induced by static discharges. Always ground both the internal and external conductors
of the cable to release static before connecting.
Proper Use of Solvent
Please use the solvent in compliance with instructions specified in its supplier. If
necessary, wear protective clothing, gloves and glasses to avoid any injuries.
Handling Safety
Please handle with care during transporting to avoid damages to surface, panel and
parts resulting from the falling from your hand.

SPECTRO-UV600 SERIES User’s Guide
V
Safety Terms and Symbols
Terms Used in this Manual. These terms may appear in this manual:
WARNING
Warning statements indicate the conditions or practices that could
result in injury or loss of life.
CAUTION
Caution statements indicate the conditions or practices that could result
in damage to this product or other property.
Terms Used on the Product. These terms may appear on the Product:
DANGER
indicates an injury or hazard may immediately happen.
WARNING
indicates an injury or hazard may be accessible potentially.
CAUTION
indicates a potential damage to the instrument or other property
might occur.
Symbols Used on the Product. These symbols may appear on the product:
Hazardous
Voltage
Protective
Earth
Terminal

SPECTRO-UV600 SERIES User’s Guide
VI
SPECTRO-UV600 Overview
SPECTRO-UV600 series is a double-beam UV-VIS spectrophotometer developped by
us and consists of three models (SPECTRO-UV690/UV670/UV650). SPECTRO-UV600
which performs qualitative, quantitative or structral analysis of material according to
the selected absorption of the light within a certain wavelength range by the
molecule or ion of the material, is mainly used in pharmacy, medical health,
chemistry, environmental protection, geology and teaching.
Main features:
Double-beam UV-VIS spectrophotometer. Through the reference light path, the
influence of light source fluctuation can be effectively reduced.
Use deuterium lamp and tungsten lamp as light source to provide 190nm to
900nm wavelength. The lamp interchange is realized through the rotation of the
condenser lens driven by the step motor.
Set the lamp interchange wavelength via the Ultra UV software and the range is
from 320nm to 380nm.
SPECTRO-UV690 and SPECTRO-UV670 provide 6 bandwidth options
(0.1nm/0.2nm/0.5nm/1nm/2nm/4nm) for your various measurement
requirements.
SPECTRO-UV690 is configured with double monochromators to effectively
reduce the stray light.
Use plane diffraction grating with high resolution as the monochromator grating.
The main monochromator of SPECTRO-UV690 is driven by the lead screw
structure, thus providing high precision.
Use chopper mirror for light splitting. The sample light path and the reference
light path are all complete and the light energy is high. Driven by the motor, the
chopper mirror transmits the sample light beam and the reference light beam
into the sample compartment and receiving compartment separately, thus only
one receiver is required and the measurement error due to the difference in
receiver nature can be excluded.
Use cuvette with variable optical path lengths as the sample cell. Glass cuvette
and quartz cuvette are available.
Configured with single cell. You can purchase the 8-series cell (option) to
measure multiple samples in one measurement.
Use photomultiplier with high sensitivity as the reciever.
Low stray light to ensure the accuracy and effectiveness of the measurement.
Provide up to ±0.3nm wavelength accuracy and 0.1nm wavelength repeatability.

SPECTRO-UV600 SERIES User’s Guide
VII
Provide high photometric accuracy and photometric repeatability.
Provide good baseline flatness and baseline drift as well as low photometric
noise.
Various standard interfaces: RS232, LAN and USB Device.
Configured with Ultra UV software to easily control SPECTRO-UV600. Provide
various measurements including spectral measurement, kinetic measurement,
photometric measurement and quantitative measurement. Besides, the software
also supports report template editing, log record function, management and
GLP mode.
The components of the instrument are independent and can be dissembled
separately.

SPECTRO-UV600 SERIES User’s Guide
VIII
Document Overview
Chapter 1 Quick Start
This chapter provides an overview of SPECTRO-UV600 series UV-VIS
spectrophotometer and detailed introductions of the instrument appearance and
components.
Chapter 2 Installation
This chapter introduces the environment and installation place requirements,
installation notices and installation procedures of SPECTRO-UV600 series UV-VIS
spectrophotometer. Besides, it introduces the power-on self-test and performance
inspection after instalaltion of the instrument.
Chapter 3 Daily Maintenance
This chapter provides the operation method of daily maintenance of the instrument
and guides users to replace and clean some of the components.
Chapter 4 Troubleshooting
This chapter lists the possible failures of the instrument during operation as well as
the corresponding solutions to help users to solve and analyze the common
problems.
Chapter 5 Specifications
This chapter lists the general characteristics and performance specifications of the
instrument.
Chapter 6 Appendix
This chapter provides information about the accessories and options, working
principles, solvent knowledge as well as warranty.

Content
SPECTRO-UV600 SERIES User’s Guide
IX
Content
Guaranty and Declaration .........................................................................II
Safety Requirement ................................................................................ III
General Safety Summary........................................................................... III
Safety Term s and Symbols .......................................................................... V
SPECTRO-UV600 Overview ...................................................................... VI
Document Overview.............................................................................. VIII
Chapter 1 Quick Start..............................................................................1-1
Appearance ............................................................................................ 1-2
Front Panel ...................................................................................... 1-2
Rear Panel ....................................................................................... 1-3
Vertical View .................................................................................... 1-4
Upward View.................................................................................... 1-5
Components ........................................................................................... 1-6
Optical System ................................................................................. 1-6
Power Source ..................................................................................1-12
Main Control Panel ...........................................................................1-12
Preamplifier Circuit Board .................................................................1-13
Chapter 2 Installation .............................................................................2-1
Installation Preparations........................................................................... 2-2
Working Environment and Place Requirements..................................... 2-2
Unpacking & General Inspection......................................................... 2-4
Installation Guide .................................................................................... 2-5
To Connect the Data Cable................................................................. 2-5
To Connect the Power Cord................................................................ 2-5
Power-on and Self-test ...................................................................... 2-5
Performance Inspection after Installation................................................... 2-7
Chapter 3 Daily Maintenance ..................................................................3-1
Daily Maintenance ................................................................................... 3-2
Replaceable Parts .................................................................................... 3-4
To Replace the Fuse .......................................................................... 3-4
To Replace the Deuterium Lamp......................................................... 3-5

Content
SPECTRO-UV600 SERIES User’s Guide
X
To Replace the Tungsten Lamp ...........................................................3-8
Cleaning................................................................................................3-10
To Clean the Exterior Surface............................................................3-10
To Clean the Cuvette........................................................................3-11
Chapter 4 Troubleshooting ..................................................................... 4-1
Chapter 5 Specifications......................................................................... 5-1
General Characteristics.......................................................................5-1
Specifications ....................................................................................5-2
Chapter 6 Appendix................................................................................ 6-1
Appendix A: SPECTRO-UV600 Series Accessories.........................................6-1
Appendix B: Theories Overview .................................................................6-2
Appendix C: Solvent Knowledge ................................................................6-5
Appendix D: Warranty ..............................................................................6-6
Index......................................................................................................... 1

Chapter 1 Quick Start
SPECTRO-UV600 User’s Guide
1-1
Chapter 1 Quick Start
This chapter guides users to get a quick understanding of the appearance and
components of SPECTRO-UV600 series UV-VIS spectrophotometer.
Subjects in this chapter:
Appearance
Components

Chapter 1 Quick Start
SPECTRO-UV600 SERIES User’s Guide
1-2
Appearance
Front Panel
Figure 1-1 Front Panel
The instrument state indicator at the front panel indicates the running state of the
instrument and is displayed in three colors.
Red: power is switched on but the instrument has not been started or error occurs;
Yellow: the instrument is in normal start-up;
Green: the instrument is successfully started and is running.
Note: the instrument state indicator is displayed in green and red alternatively during
version update.
State Indicator

Chapter 1 Quick Start
SPECTRO-UV600 SERIES User’s Guide
1-3
Rear Panel
Figure 1-2 Rear Panel
The rear panel consists of the power switch, fuse, AC power socket, fan and
communication interfaces.
Power Switch: control both the lamp power and system power.
Fuse Seat: AC 250V, T3A fuse.
AC Power Socket: connect the instrument to specified AC power using the power
cord provided in the accessories to energize the whole system. SPECTRO-UV600
can accept 220VACRMS±22VACRMS, 50Hz A C p o w e r.
Fan: SPECTRO-UV600 uses fan cooling. Besides, it provides over-temperature
protection function. When the system temperature is too high, the beeper will
sound and the instrument state indicator at the front panel will turn red.
Communication Interfaces: provide RS-232, LAN and USB-Device interfaces. For
detailed information of each interface, refer to the introduction in “Main Control
Panel”.
Power Switch AC Power Socket Fan RS-232 LAN
Fuse Seat USB-Device

Chapter 1 Quick Start
SPECTRO-UV600 SERIES User’s Guide
1-4
Vertical View
Figure 1-3 Vertical View
Keep the sample compartment cover installed during the measurement to avoid
affecting the measurement accuracy. If the sample compartment cover is no installed,
the beeper will sound and the instrument state indicator will turn red.
Light Source
Compartment
Sample
Compartment

Chapter 1 Quick Start
SPECTRO-UV600 SERIES User’s Guide
1-5
Upward View
As shown in the figure below, there are four round rubber feet at the four corners of
the bottom of the case of SPECTRO-UV600 which make the instrument be stably
placed on the workbench.
Figure 1-4 Upward View
Rubber Feet

Chapter 1 Quick Start
SPECTRO-UV600 SERIES User’s Guide
1-6
Components
As shown in Figure 1-5, SPECTRO-UV600 mainly consists of four systems (optical
system, power, signal acquisition and amplification system and main control panel).
The main components and functions of each system are introduced in details in the
following part.
Figure 1-5 SPECTRO-UV600 Working Process
Optical System
The optical system is the core component of the instrument and detects the absorbing
degree of the light by the sample. The optical system of SPECTRO-UV600 mainly
consisits of the light source compartment, optical filter set, monochromator, light
splitting compartment and recieving compartment. The working principles of the
optical system of SPECTRO-UV600 are as shown in Figure 1-6 and Figure 1-7.

Chapter 1 Quick Start
SPECTRO-UV600 SERIES User’s Guide
1-7
Figure 1-6 Optical Configurations of SPECTRO-UV690
Ta b l e 1-1 Optical System of SPECTRO-UV690
No.
Description
No.
Description
No.
Description
1
Deuterium Lamp
10
Collimating Mirror
19
Reflectoscope 3
2 Tungsten Lamp 11 Main
Monochromator
Grating
20 Condenser Lens 7
3
Condenser Lens 1
12
Condenser Lens 4
21
Reference Cell
4
Light Diaphragm
13
Exit Slit
22
Sample Cell
5
Condenser Lens 2
14
Condenser Lens 5
23
Condenser Lens 8
6 Pre-
monochromator
Grating
15 Reflectoscope 1 24 Condenser Lens 9
7
Condenser Lens 3
16
Chopper Mirror
25
Reflectoscope 4
8
Optical Filter Set
17
Reflectoscope 2
26
Photomultiplier
9
Entrance Slit
18
Condenser Lens 6
9
1
2
3
4
12
11
10
19
14
18
15
20
16
5
6
7
8
13
23
25
24
26
17
21
22

Chapter 1 Quick Start
SPECTRO-UV600 SERIES User’s Guide
1-8
Figure 1-7 Optical Configurations of SPECTRO-UV670/UV650
Ta b l e 1-2 SPECTRO-UV670/UV650 Optical System
No.
Description
No.
Description
No.
Description
1
Deuterium Lamp
9
Collimating Mirror
17
Reflectoscope 3
2
Tungsten Lamp
10
Condenser Lens 3
18
Condenser Lens 6
3
Condenser Lens 1
11
Exit Slit
19
Reference Cell
4
Light Diaphragm
12
Condenser Lens 4
20
Sample Cell
5
Condenser Lens 2
13
Reflectoscope 1
21
Condenser Lens 7
6
Optical Filter Set
14
Reflectoscope 2
22
Reflectoscope 4
7
Entrance Slit
15
Chopper Mirror
23
Condenser Lens 8
8
Grating
16
Condenser Lens 5
24
Photomultiplier
11
14
6
10
8
9
23
24
21
22
1
2
3
4
12
16
13
18
15
5
7
17
20
19

Chapter 1 Quick Start
SPECTRO-UV600 SERIES User’s Guide
1-9
Light Source Compartment
It mainly consists of the deuterium lamp, tungsten lamp and condenser lens 1 and
provides compound light with 190nm to 900nm wavelength. Wherein, the deuterium
lamp provides compound light with 190nm to 400nm wavelength and the tungsten
lamp provides compound light with 400nm to 900nm wavelength.
Condenser lens 1 is driven by the step motor to realize the interchange between the
deuterium lamp and tungsten lamp. Users can set the interchange wavelength via the
Ultra UV software and the range available is from 320nm to 380nm (for details, please
refer to the Ultra UV User’s Guide).
The converging light going out of the light source compartment is focused on the light
diaphragm and then is focused on the entrance slit of the monochromator through
condenser lens 2.
Optical Filter Set
The optical filter set is mainly used to filter out the stray light and the multi-stage
spectrum of the grating.
SPECTRO-UV600 provides 5 optical filter positions (wherein, the first is blank scale
and do not contain optical filter) covering light source with 190nm to 900nm
wavelength.
The optical filters are distributed on the optical filter plate which is driven by the step
motor to realize optical filter interchange, thus selecting different filter range to
remove the stray light.
The filter ranges of the 5 optical filters:
Blank: 190nm to 305nm
Purple: 305nm to 370nm
Blue: 370nm to 550nm
Green: 550nm to 700nm
Black: 700nm to 900nm

Chapter 1 Quick Start
SPECTRO-UV600 SERIES User’s Guide
1-10
Monochromator
The main use of the monochromator is to convert compound light into monochromatic
light. The monochromator mainly consists of the entrance slit, exit slit, collimating
mirror, plane grating and condenser lens.
The main function of each component is as follows:
Entrance slit and exit slit: limit the bandwidth. The variable slit set provides
different bandwidth options;
Collimating mirror: convert the diverging light from the entrance slit to parallel light;
Plane grating: convert the compound light to monochromatic lights and emit them
at different angles. The rotation angle of the grating corresponds to the wavelength;
Condenser lens: convert the parallel light to converging light and focus it to the exit
slit.
SPECTRO-UV690 is configured with 2 monochromators (the pre-monochromator and
the main monochromator). Wherein, the pre-monochromator consists of condenser
lens 2, condenser lens 3 and pre-monochromator grating; the main monochromator
consists of the entrance slit, collimating mirror, main monochromator grating,
condenser lens 4 and exit slit. The pre-monochromator is driven by the step motor
while the main monochromator is driven by the lead screw to select the emergent
light wavelength.
SPECTRO-UV670 and SPECTRO-UV650 are configured with a monochromator
consisting of the entrance slit, collimating mirror, grating, condenser lens 3 and exit
slit. The grating is driven by the step motor to select the emergent light wavelength.
Note: compared to SPECTRO-UV670 and SPECTRO-UV650, SPECTRO-UV690 is
configured with one more monochromator to reduce the stray light.
At the entrance and exit of the monochromator are the entrance slit and exit slit.
SPECTRO-UV690 and SPECTRO-UV670 provide 6 bandwidth options (include 0.1nm,
0.2nm, 0.5nm, 1nm, 2nm and 4nm); while the bandwidth of SPECTRO-UV650 is fixed
at 2nm.