Nikon eclipse e100 User manual

Microscope
ECLIPSE E100
Instructions
M413 E 06.10.NF.1

1
Thank you for purchasing this Nikon product. This instruction manual is for the users of the
Nikon Microscope ECLIPSE E100 describing basic operation of the microscope. To ensure
correct usage, please read this manual thoroughly before using the microscope.
• It is prohibited to reproduce or transmit this manual in any form without the prior
consent of Nikon.
• The instructions and specifications in this manual are subject to change without
notice.
• Although every effort has been made to ensure the accuracy of this manual, if
you find that any part of this manual is unclear or incorrect, contact your nearest
Nikon representative.
• Some ECLIPSE E100 microscope sets may come with different components
and accessories from those shown in this manual.
• Also read the manuals for the products used with the microscope, for example,
the Nikon photomicrographic equipment.

2
CAUTION
WARNING
Warning and Caution Symbols Used in This Manual
Symbols Labeled on the Product
Though Nikon products are designed to provide you with utmost safety during use, incorrect usage or
disregard of the instructions may cause personal injury or property damage. For your own safety, read the
instruction manual carefully and thoroughly before using the product. Do not discard this manual. Always
keep it near the product for easy reference.
Inside this instruction manual, safety instructions are indicated with the symbols shown below. Be sure to
follow the instructions marked with these symbols for your safety.
Symbol Meaning
Disregarding instructions marked with this symbol may lead to death or
serious injury.
Disregarding instructions marked with this symbol may lead to injury or
property damage.
Symbol Meaning
Caution! Hot surface
This symbol label attached near the field lens unit (the lamp is set
underneath the field lens unit) and on the bottom surface of the base plate
(the lamp is set above this place) reminds you of the following.
WARNING: Risk of burn injury. The lamp and its surroundings (including
the field lens unit) become hot during use. Do not touch them
while the lamp is on and for thirty minutes after the lamp is
turned off.
CAUTION: The lamp and its surroundings remain hot even after the lamp
is turned off. Make sure that they are sufficiently cool before
replacing the lamp.
Caution!
This symbol label attached near the AC inlet reminds you of the following.
CAUTION: Make sure that the input voltage set by the voltage selector
matches the voltage provided in your region.
The voltage selector on the microscope sent to the United
States is set to “120V” at the factory.

3
WARNING
WARNING
WARNING
WARNING
Common Sense Safety Instructions
1. Purpose
Use this microscope only for microscopic observation.
Do not use this microscope for any other purpose because the protection provided
by the equipment may be impaired.
2. Never Disassemble the Microscope
There are no user serviceable parts inside the microscope. Disassembly will void the warranty, and
could degrade the performance, cause electrical shock or personal injury, or damage the instrument.
If you have a service problem, contact your nearest Nikon representative.
4. Use the Specified Lamp, Fuse, and Power Cord
Use the specified lamp and fuse. For the microscope with the nameplate showing [100/110/120 V], use
the power cord provided. For the microscope with the nameplate showing [220/230/240 V], use the
specified power cord. Using an incorrect lamp, fuse, or power cord may damage the instrument or cause
a fire. (Also see p. 41-42 on power cord.)
●Specified Lamp
Halogen lamp 6V-20W (PHILIPS 7388 or OSRAM HLX64250)
●Specified Fuse
• For the microscope with the nameplate showing [100/110/120 V 〜 〜]:
250 V, 1 A, time-lag low-breaking type, 5X20 miniature fuses (two pieces)
• For the microscope with the nameplate showing [220/230/240 V 〜 〜]:
250 V, 0.5 A, time-lag low-breaking type, 5X20 miniature fuses (two pieces)
3. Check the Input Voltage
The input voltage is indicated in two places at the rear of the microscope: on the nameplate and above
the AC-IN socket. Confirm that these input voltage indications correspond to the voltage provided in your
region. If not, follow one of the instructions below. The use of microscopes with the different input
voltage indications will cause overcurrent and overheating, which may result in fire or severe damage to
the microscope.
●If the voltage indication on the nameplate differs:
Do not plug in the microscope. Contact your nearest Nikon
representative.
●If the voltage indication above the AC-IN socket differs:
Refer to p. 26 and change the input voltage setting before
turning on the power switch.
• For the microscope with the nameplate showing
[100/110/120 V 〜 〜]:
The voltage can be set to: AC 100 V, 110 V or 120 V.
• For the microscope with the nameplate showing
[220/230/240 V 〜 〜]:
The voltage can be set to: AC 220 V, 230 V or 240 V.
Input Voltage
Indications

4
5. Turn Off Power Switch before Assembling the
Microscope, Replacing the Lamp or Fuse, and
Plugging in or Unplugging the Power Cord
CAUTION
WARNING
CAUTION
CAUTION
Turn off the power switch before you plug or unplug the power cord to prevent electrical shock or fire.
Also turn off the power switch and then unplug the power cord before assembling the microscope, and
before changing the lamp or fuse. To turn off the power, turn the power switch to ○.
6. Keep the Microscope Free of Moisture and Foreign Matter
Keep the microscope free of moisture to prevent short circuiting that could result in overheating or other
malfunctions. If water splashes on the microscope, immediately turn off the power switch (turn the switch
to ○) and unplug the power cord. Then, wipe off the water with a dry cloth. Short circuiting can also
result when foreign matter is trapped inside the microscope. If foreign matter or water has entered the
microscope, do not use the microscope and contact your nearest Nikon representative.
7. Heat at the Light Source
The lamp becomes hot during use. Do not remove the field lens unit while the lamp is on, and be sure
the lamp has been off for 30 minutes before touching it.
• When changing the lamp bulb, make sure that the lamp is cool enough to touch (the light should be
off at least 30 minutes).
• Do not touch the lamp while it is on or until the lamp has been off for 30 minutes, as doing so could
result in burns.
• Never bring cloth, paper or flammable volatile substances such as gasoline, petroleum benzine,
acetone, thinner, or alcohol near a hot lamp, as a fire could result.
8. Stage Rack
The rack for the stage protrudes during use. Be careful not to hit the rack with your hand or other parts of
your body when handling the microscope as you may get hurt by an edge of the rack.
Rack

5
9. Installation
10. Carrying the Microscope
This microscope is a precision instrument. Using the microscope in an unfavorable environment could
result in malfunctions or degraded performance. Consider the following conditions when choosing the
installation location.
• Observation conditions are better if light from windows and bright room light can be avoided.
• Install the microscope in a location with a room temperature of 0˚ to 40˚ C and with a maximum
relative humidity of 85%. High temperature and humidity are to be avoided because they promote
mold growth and condensation, which may damage the microscope.
• Dirt and dust degrade optical performance and are to be avoided.
• Vibrations in the environment will degrade the image. Install the microscope in a location free of
vibrations.
• Install the microscope on a solid table and keep the microscope level.
• This microscope emits a feeble electromagnetic wave. Do not place a precision electronic device
near the microscope as precision could be degraded. Also, avoid placing a radio or TV near the
microscope as reception of sound and images may be hampered.
• Install the microscope in the location of 10 cm or more away from the surrounding walls.
• Install the microscope in the place that allows easy detachment of the power cord from the AC inlet
in the event of emergency.
This microscope is a precision instrument. Handle it gently.
Strong shocks and forcible operation will damage the
instrument. Shocks to the objectives, especially, could degrade
image precision.
• When carrying the microscope, hold it at its arm with both
hands.
• Do not hold the focus knobs, the eyepiece tube, or the
stage. These parts could easily come off and could result
in malfunctions.
11. Handling the Lamp
Do not touch the glass part of the lamp with bare hands. Wear gloves or use a cloth when handling the
lamp so as not to leave fingerprints on the surface. Wipe off any fingerprints or stains using a clean
cloth moistened with alcohol. Fingerprints will etch into the hot surface of the lamp and reduce the
brightness, damage the lamp or reduce its service life.
Handle the lamp gently. Shocks and vibrations will damage the lamp or reduce its service life.
When changing the lamp, be sure that the contact is not damaged. If the contact is damaged, the lamp
may not light up or may overheat. Insert the lamp’s contact pins fully into the socket holes. If the pins
are loose, the lamp could come off or result in a contact failure, which will cause overheating or smoke.
Also, make sure that the field lens unit is securely attached.

6
12. Focus Knobs
Do not turn the right and left focus knobs simultaneously in opposite directions. Do not turn the coarse
focus knob any further after the stage has been moved up or down to its limit. These operations will
damage the focusing mechanism.
14. Shipping Clamps
The microscope is held tightly by the clamps during shipment.
Be sure to remove the clamps before use. For details, see p. 27.
Do not turn the knobs in opposite directions.
WARNING
13. Oil-Immersion Observation
Use only a minimum quantity of oil. If too much oil is applied, surplus oil could flow out to the stage and
the condenser which could lead to degraded performance.
When using petroleum benzine or absolute alcohol to wipe off immersion oil or
to clean the lenses, follow the instructions provided by their manufacturers.
Absolute alcohol and petroleum benzine are inflammable. Take great care
when handling them.
Shipping Clamps

7
Contents
Warning and Caution Symbols Used in This Manual ････････････････････････････2
Symbols Labeled on the Product ･････････････････････････････････････････････2
Common Sense Safety Instructions･･･････････････････････････････････････････3
I. Nomenclature of Each Part ･･･････････････････････････････････････････8
II. Switches and Controls･･･････････････････････････････････････････････10
III. A Swift Microscopic Procedure ･････････････････････････････････････12
IV. Microscopy (Detailed Procedure) ･･･････････････････････････････････14
V. Miscellaneous Operations ･･･････････････････････････････････････････22
1. Oil-Immersion Observation ･･･････････････････････････････････････････････22
2. Adjusting the Torque of the Coarse Focus Knob ････････････････････････････24
3. Photomicrography and TV Microscopy･････････････････････････････････････24
4. Cord Hangers ･･････････････････････････････････････････････････････････24
VI. Assembly ･････････････････････････････････････････････････････････････25
1. Assembly of Standard Set････････････････････････････････････････････････26
2. Assembly of Additional Components･･･････････････････････････････････････28
1) Condenser･･･････････････････････････････････････････････････････････28
2) Objectives ･･･････････････････････････････････････････････････････････29
3) Specimen Holder ･････････････････････････････････････････････････････29
4) Eyepiece and Eyepiece Pointer･････････････････････････････････････････29
5) Monocular eyepiece tube ･･････････････････････････････････････････････29
6) Other Accessories ････････････････････････････････････････････････････29
3. Replacement of Consumable Materials ････････････････････････････････････30
1) Replacing the Lamp･･･････････････････････････････････････････････････30
2) Replacing the Fuse ･･･････････････････････････････････････････････････31
VII. Optical Characteristics ･･････････････････････････････････････････････33
VIII.Troubleshooting tables ･･････････････････････････････････････････････35
IX. Care and Maintenance ･･･････････････････････････････････････････････40
X. Specifications ････････････････････････････････････････････････････････41

8
The microscope is made up of the following components.
Basic unit
Eyepieces
Screwed on to the eyepiece tube.
Eyepiece tube
This is a binocular eyepiece tube. A trinocular eyepiece tube is available for
photomicrography and TV microscopy. Amonocular eyepiece tube can be used also.
Objectives
Objectives with various magnifying powers are available.
Condenser
Used for condensing light. The condenser should be positioned slightly lower
than its upper limit.
Adjust the aperture diaphragm lever according to the objective.
Field lens unit
Pull the field lens unit upward when changing lamp.
Lamp
Halogen lamp of 6 V-20 W is used.
Fuse
For the microscope with the nameplate showing [100/110/120 V], 250 V, 1 A, time-lag low-
breaking type, 5X20 miniature fuses (two pieces) are used.
For the microscope with the nameplate showing [220/230/240 V], 250 V, 0.5 A, time-lag low-
breaking type, 5X20 miniature fuses (two pieces) are used.
Power cord
Use the power cord provided.
Nomenclature of Each Part
1
2
3
4
5
6
7
8
9

9
1 Basic unit
2Eyepieces 3Eyepiece tube
4Objectives
5Condenser
6Field lens unit
7Lamp
8Fuse (x2)
9
Power cord

Diopter ring
Adjust the diopter ring to compensate for
the difference between your right and left
eyesight. (p. 18)
Revolving nosepiece
Can hold up to four objectives.
Specimen holder
Put your finger at the root tilt of the claw to
open the claw. (p. 15, 29)
Magnifying power indication ring
Condenser aperture diaphragm lever
Set the lever to match the magnifying
power of the objective. (p. 20)
Blue filter and the filter holder
Stage
Power switch
When turned to |, power is turned on and
the lamp lights.
When turned to ○, power is turned off and
the lamp goes off.
Brightness control dial
When turned clockwise, the lamp voltage
increases and the viewfield becomes
brighter.
When turned counterclockwise, the lamp
voltage decreases and the viewfield
becomes darker.
Longitudinal stage motion (Y Axis)
knob
These knobs are located either to the right
or the left of the stage.
Lateral stage motion (X Axis) knob
ditto
Fine focus knob
Condenser clamp screw
10
Condenser vertical motion lever
Use this lever to adjust the vertical
position of the condenser. (p. 19)
This lever is located either on the right or
the left side of the stage.
Coarse focus knob torque adjustment
ring
Used to adjust the tension (torque) of the
coarse focus knob. (p. 24)
Coarse focus knob
There are both coarse and fine focus
knobs on the opposite side of the stage
motion knobs.
Fine focus knob
Used for focusing. There is no coarse
focus knob on the side with the stage
motion knobs.
Voltage selector (Fuse holder)
Use this selector to match the input
voltage of the microscope to the voltage
provided in your region. (p. 26)
Nameplate
Indicates the input voltage.
AC-IN socket
Plug the power cord into this inlet. Make
sure that the power switch is off (turned to
○) before plugging the cord in.
Switches and Controls
1
2
3
4
5
6
7
8
9
10
11
12
20
18
19
17
16
15
14
13

11
1Diopter ring
7Stage
3Specimen holder
5Condenser aperture
diaphragm lever
13
Condenser clamp
screw
6Blue filter and the
filter holder
20
AC-IN socket
18
Voltage selector
(Fuse holder)
19
Nameplate
12
Fine focus knob
9Brightness control dial
10
Longitudinal stage
motion (Y Axis) knob
11
Lateral stage motion
(X Axis) knob
17
Fine focus knob
8Power switch
4Magnifying power
indication ring
16
Coarse focus knob
15
Coarse focus
knob torque
adjustment ring
14
Condenser vertical motion lever
2Revolving nosepiece

12
A Swift Microscopic Procedure
3Adjust brightness too.
Focus with 10x objective.
Turn on the lamp and adjust
interpupillary distance.
Viewfields
2Place specimen slide on
the stage.
(Coverglass up.)
3Swing the 10x in the
optical path.
Use this knob for focusing.
1Turn on the power switch.
2Widen or narrow to merge
the viewfields into one.
1Match the
bottom edge
of the diopter
ring with the
engraved
base line.

13
Magnify the image and
observe!
Adjust the diopter.
2Switch back to the 10x.
Use your right eye.
3Hold the 10x. Use your left eye.
1Switch to the 40x.
And focus
with this ring.
Then focus
with this ring.
1Check the magnifying power.
Repeat the steps
1
to
3
twice.
Repeat the steps 1 to 3 .
2Move the lever to the
magnifying power of the
objective.
3Observe.
Turn off the power.
Wait till the microscope cools down
before storing.
Focus with this knob.

14
Microscopy (Detailed Procedure)
1) Lamp Illumination
3) Align the Diopter Ring with the Engraved Base Line
Turn on the power switch (turn to |) and the
lamp will come on. Turn the brightness control
dial to adjust the brightness of the viewfield.
(Turning the dial clockwise increases the
brightness; turning the dial counterclockwise
decreases it.)
Turn the diopter ring on the right eyepiece to
align its bottom edge with the engraved base
line. Turn and align the diopter ring on the left
eyepiece in the same way.
2) Interpupillary Distance
Adjustment
Adjust the distance between the eyepieces to
merge the right and left viewfields into one.
(This is an adjustment to match the distance
between eyepieces with the distance between
your eyes).
Power ON
Darkens. Brightens.
Merge the right and left
viewfields into one.
Match the bottom edge of the diopter ring
with the engraved base line.

15
4) Specimen Mounting
Place specimen slide on the stage with the
coverglass facing upward. Open the claw of
the specimen holder with your finger at the root
tilt and fix the specimen slide with the claw.
5) Focus with the 10x Objective
Rotate the revolving nosepiece to bring the 10x
objective into the optical path. (The objective
will click into place when rotated into position.)
Bring the specimen image into focus by turning
the coarse focus and then fine focus knob.
• Direction of stage movement relative to focus
knob rotation is shown in the figure.
• There is no coarse focus knob on the side
with the stage motion knobs. There are both
coarse and fine focus knobs on the opposite
side of the stage motion knobs.
• Do not turn the right and left focus knobs
simultaneously in the opposite directions. Do
not turn the coarse focus knob further after the
stage has reached its lower or upper limit.
These operations could result in a
malfunction.
Coverglass
Turn the focus knob as shown to move the
stage down.

16
Focusing
Turning the focus knobs recklessly is a long and hard way to focus on the image. If you are
using a high power objective, you may even damage the specimen by pressing it against the
objective. Before breaking the coverglasses or damaging the objectives, read the following
and find the correct way to focus on the specimen.
(1) Put the 10x (or 4x) objective in the optical path.
(2) Turn the coarse focus knob to raise the stage to its upper limit. *1
(3) Looking into the eyepieces, slowly rotate the coarse focus knob to lower the
stage. When the specimen image appears, stop rotating the knob.
(4) Rotate the fine focus knob and precisely focus on the image.
When you want to observe the image with a high power objective, first focus on
the image using a 10x (or 4x) objective. Then change to a high power objective
and rotate the fine focus knob for precise focusing.
then look into the eyepieces
and lower the stage.
1. When rotating the coarse focus knob while looking into the eyepieces, be sure to
turn it only in the direction that lowers the stage.
2. When raising the stage using the coarse focus knob, take your eyes off the
eyepieces and look at the gap between the upper surface of the specimen and the
front of the objective from the side.
3. First focus with a low power objective. Then change to a high power objective.
*1: Since the working distances of 10x and 4x objectives are large (p. 33), these objectives do not touch
the specimen even when the stage is raised to its upper limit provided that the slide and coverglasses
of a standard thickness are used. (The standard thickness for slides is 1.2 mm and that for coverglass
is 0.17 mm.)
First raise the stage to the most extent
not to hit against the objective,

17
Using the Working Distance for Focusing
Each objective has its working distance indicated on its side. The working distance is the
distance between the front of the objective and the specimen when the specimen image is in
focus. If you have difficulties in focusing with the standard procedure described on p. 16, try
one of the following methods using the working distance for focusing.
Method 1:
While looking at the microscope from the side, rotate the coarse focus knob to bring the
specimen close to the objective. When the distance between the specimen and the front of the
objective becomes slightly smaller than the working distance, take your hands off the coarse
focus knob. The specimen is now almost in focus. Look into the eyepieces and rotate the fine
focus knob in the direction that lowers the stage.
Method 2:
Swing the 40x objective into the optical path. While looking at the microscope from the side,
rotate the coarse focus knob until the specimen almost touches the objective (about 0.5 mm
apart from the front of the objective). The specimen is now almost in focus. Switch to the 10x
objective, look into the eyepieces, and rotate the fine focus knob slightly to find the focal point.
Be careful not to hit the objective with the specimen.
Raise the stage until this
distance is slightly smaller
than the working distance.
Lower the stage with the fine
focus knob to find the focal
point.

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6) Eyepiece Diopter Adjustments
Adjust the diopter ring on the eyepiece tube according to the difference between your left and right
eyesight. This adjustment enables the user to take full advantage of the high-quality objectives,
including their parfocality.
(1) Swing the 40x objective in the optical
path. Rotate the coarse and then fine
focus knobs to bring the specimen in
focus.
(2) Switch back to the 10x (or 4x) objective.
While looking into the right eyepiece
with your right eye, focus on the
specimen by rotating the right diopter
ring and not using the focus knob.
(3) While looking into the left eyepiece with
your left eye, focus on the specimen by
rotating the left diopter ring and not
using the focus knob.
(4) Repeat the steps (1) to (3).
Focus with this knob.
Then focus with this ring
Use your left eye.
And focus with this ring.
Use your right eye.
Switchtothe40×.
Switchbacktothe10×.
Holdthe10×.