Olympus BX51WI User manual

INSTRUCTIONS
BX51WI
FIXED-STAGE UPRIGHT
This instruction manual is for the Olympus Fixed-Stage Upright Microscope Model BX51WI. To
ensure the safety, obtain optimum performance and to familiarize yourself fully with the use of
this microscope, we recommend that you study this manual thoroughly before operating the
microscope. Retain this instruction manual in an easily accessible place near the work desk for
future reference.
MICROSCOPE
A X 7 6 5 7

BX51WI
CONTENTS
3
4-7
8, 9
10-18
IMPORTANT — Be sure to read this section for safe use of the equipment. —
1 MODULE NOMENCLATURE
2 CONTROLS
3
4 USING THE CONTROLS
TRANSMITTED LIGHT BRIGHTFIELD OBSERVATION PROCEDURE
4-1 Microscope Base, Power Supply Unit (TH4)................................................................................................ 10
4-2 Focusing Block ........................................................................................................................................................................................ 11
1Controlling the Light Intensity (TH4) 2Using the Filter Turret
4-3 Stage (IX-SVL2) ............................................................................................................................................................................. 12, 13
4-4 Revolving Nosepiece .................................................................................................................................................................... 13
4-5 Observation Tube ..................................................................................................................................................................... 14, 15
1Adjusting the Interpupillary Distance 2Adjusting the Diopter
3Using the Eye Shades 4Using Eyepiece Micrometer Disks
5Selecting the Light Path of Trinocular Tube
1Switching the Objectives (U-SLRE, WI-SRE3)
1Using the Pre-focusing Lever 2Using the Fine Adjustment Fast-Feed Knob
3Using the Frost Switching Lever
4 Adjusting the Coarse Adjustment Knob Rotation Tension
1-2
Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to
assemble the microscope yourself, please read Chapter 9, “ASSEMBLY” (pages 36 to 42) carefully. For the modules
provided with instruction manuals, also read the assembly procedures in their instruction manuals.
1Placing the Specimen 2Moving the Specimen
3Setting the Grounding 4
Adjusting the X-Axis/Y-Axis Knob Rotation Tension
5Using the Light Shield Sheet 6Lowering the Stage Height
4-6 Condenser .......................................................................................................................................................................................... 16, 17
1Centering the Condenser (Field Iris Diaphragm, Aperture Iris Diaphragm)
2Oblique Illumination (WI-OBCD)
4-7 Immersion Objectives .................................................................................................................................................................. 18
1Using Water Immersion Objectives (Water Immersion Cap for XL Objectives XL-CAP)

5 OTHER OBSERVATION METHODS
5-1 Differential Interference Contrast Observation............................................................................... 19-23
1Attaching the Analyzer 2Attaching the Polarizer
3Attaching the DIC Prisms (for Revolving Nosepiece)
4Attaching the DIC Prisms (for Condenser)
5Adjusting the Polarizer Position (except the U-UCD8)
6Observation Method
5-2 Reflected Light Fluorescence Observation................................................................................................... 24
5-3
Infrared Light (IR)/Differential Interference Contrast (DIC) Observation ............... 24-27
1Introduction 2Attaching the IR Modules
3DIC Observation Using IR
5-4 Macro Reflected Light Fluorescence Observation ................................................................. 28-30
1Introduction 2Attaching the Modules
3Filter Characteristics of Fluorescence Mirror Units
4 Fabricating Optional Mirror Unit
6 TROUBLESHOOTING GUIDE
7 SPECIFICATIONS
8 OPTICAL CHARACTERISTICS
9
31-33
34
35
36-42
ASSEMBLY — See this section for the replacement of the light bulb. —
PROPER SELECTION OF THE POWER SUPPLY CORD ................................................................... 43, 44
19-30
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user’s authority to operate the equipment.
This device complies with the requirements of directive 98/79/EC concerning in vitro diag-
nostic medical devices. CE marking means the conformity to the directive.
10 LAMP HOUSING INSPECTION SHEET 45

1
BX51WI
Fig. 1
IMPORTANT
This microscope employs a UIS2 (UIS) (Universal Infinity System) optical design, and should be used only
with UIS2 (UIS) eyepieces, objectives and condensers for the BX2 series. (Some of the modules designed
for the BX series are also usable. For details, please consult Olympus or the latest catalogues.)
To obtain comprehensive understanding on the operating procedures, please also read the separately
provided instruction manuals.
SAFETY PRECAUTIONS
1. After the equipment has been used in an observation of a specimen
that is accompanied with a potential of infection, clean the parts
coming in contact with the specimen to prevent infection.
· Moving this product is accompanied with the risk of dropping the
specimen. Be sure to remove the specimen before moving this
product.
· In case the specimen is damaged by erroneous operation, promptly
take the infection prevention measures.
2. Culture liquid or water spilt on the stage, condenser or microscope may
damage the equipment. Immediately wipe the liquid or water off if it is
spilt on them.
3. When moving the microscope, disconnect the reflected light illuminator,
observation tube and transmitted light lamp housing and carefully carry
the microscope by the base (front edge) @ and the grasping part on the
rear of the arm ² as shown in Fig. 1. (Weight: approx. 15 kg.)
Also be careful against slipping of hands during carrying.
# Damage to the microscope will occur if you grasp it by other parts
including the stage, coarse/fine adjustment knob, etc.
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol Explanation
Indicates that the surface becomes hot, and should not be touched with bare hands.
Before use, carefully read the instruction manual. Improper use could result in personal injury to
the user and/or damage to the equipment.
@
²
Instruction manual
BX51WI
TH4
BX-URA2/BX-RFA
WI-DPMC
WI-XYM/XYS
WI-SSNP
Contents
Explanation of transmitted brightfield observation, differential
interference contrast observation and infrared observation
Explanation of the external halogen bulb power supply unit
Explanation of reflected light fluorescence observation
Explanation of the variable-magnification dual-port observation tube
Explanation of the XY mover/bridge stage
Explanation of the swinging-sliding revolving nosepiece
1Getting Ready
1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. The U-SWTR-3 super-widefield observation tube (FN 26.5) cannot be used with the BX51WI microscope.
3. The BX51WI microscope can be used with an intermediate attachment (such as a BX-URA2 or BX-RFA reflected light
illuminator, U-ECA or U-CA magnification changer, etc.).
Two intermediate attachments can be used only in the following conditions:
· The U-CA or U-ECA magnification changer or U-FWO filter wheel can be mounted as the second attachment.
· When a TV adapter with 1X or higher power is used, 2/3-inch CCD TV observation is possible.
· The peripheral areas of the field of view may be obscured or cut off in binocular observation using the U-TR30-2, U-ETR
or U-TR30IR (FN 22) super-widefield observation tube.

2
2Maintenance and Storage
1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and
wipe gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
alcohol.
Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks –– for example, electrical
equipment that is being switched on or off.
Also remember to always use it only in a well-ventilated room.
2. Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean
them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Never attempt to disassemble any part of the microscope.
4. When not using the microscope, make sure to set the main switch to “ ” (OFF), confirm that the lamp housing is cool
enough and cover the microscope with the provided dust cover.
5. When disposing of this unit, check the regulations and rules of your local government and be sure to observe them.
3 Warning Indication
A warning sticker is attached to a part where special precaution is required when handling and using the system. Always
heed the warning.
Warning indication
position
Lamp housing (U-LH100-3/U-LH100IR)
(Warning against high temperature)
4Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).
5Intended use
This instrument has been designed to be used to observe magnified images of specimens in routine and research
applications.
Do not use this instrument for any purpose other than its intended use.
4. In IR (infrared) observation, the U-CA or U-ECA magnification changer can be used only when the U-ETR3 or U-TR30IR
observation is used.
5. In photomicrography with visible light, correct exposure may be impossible if the microscope is set for IR observation.
Be sure to engage the provided IR cut filter (light blue) before photomicrography.
6. When the XLUMPlanFl20XW objective is used, only the U-TV1X-2, U-TVCAC, U-PMTVC2XIR or U-PMTVC4XIR TV adapter
can be used.
7. Do not attempt to remove or loosen the click springs and screws. Otherwise, Olympus can no longer warrant the
performance of the microscope.
The clicking force of the revolving nosepiece has been set weak in order to reduce vibrations during objective switching.
To reproduce the correct click position, switch the objectives gently by operating the lever.
8. Caution for use of the U-ETR3 upright trinocular tube:
When the aperture stop of the condenser is reduced using a reflected light fluorescence illuminator and the LUMPlanFl60XW
objective, part of the observed field of view may be obscured slightly. This is due to the reduction of the light intensity in
the field of view due to the narrow aperture and is not due to a defective optical adjustment of the microscope.
This phenomenon does not affect the photomicrography or TV camera light path.

3
BX51WI
MODULE NOMENCLATURE
}The modules shown below are only the representative modules. As there are other modules which can be combined with
the microscope but are not shown below, please also refer to the latest Olympus catalogues or your dealer.
Reflected Light Mercury Lamp Housing
· U-LH100HG
· U-LH100HGAPO
Transmitted Arm BX-ARM
or Reflected Light Fluorescence Illuminator
· BX-URA2
· BX-RFA
Trinocular Observation Tube
· U-TR30-2 (FN 22)
· U-TR30IR (FN 22)
· U-ETR3 (FN 22)
Revolving Arm
WI-NPA
Revolving Nosepiece
· WI-SRE3
· WI-SNPXLU2
· U-SLRE
Cross Stage
IX-SVL2
or commercially
available bridge
stage
Fixed-Stage Adapter
WI-FSH
Condenser
· WI-UCD
· WI-DICD
· WI-OBCD
· U-UCD8
· U-SC3
· U-AAC
· U-AC2
Microscope Frame BX51WIF
Power Supply Unit
U-RFL-T
Transmitted Light Lamp Housing
· U-LH100-3
· U-LH100IR
Power Supply Unit (for Halogen Bulb)
TH4
Hand Switch TH4-HS
Objective
· MPLN5X
· UMPlanFLN10XW/20XW
· LUMPlanFLN40XW/60XW
· LUMFLN60XW
· LUMPlanFl100XW
· XLUMPlanFI20XW
(exclusively for use with the WI-SNPXLU2)
· XLFluor2X/340
(exclusively for use with the U-SLRE)
· XLFluor4X/340
(exclusively for use with the U-SLRE)

4
CONTROLS
}If you have not yet assembled the microscope, read Chapter 9, “ASSEMBLY” (pages 36 to 42).
Interpupillary distance
scale (Page 14)
}The descriptions on the filter turret, revolving nosepiece, condenser, etc. will be given in the subsequent pages.
Diopter adjustment
ring (Page 14)
Reflected light
fluorescence illuminator
(See the separately
provided instruction
manual.)
Stage center plate
Y-axis knob (Page 12)
X-axis knob (Page 12)
Rubber cap for fine
adjustment knob
(Page 11)
Pre-focusing lever (Page 11)
Coarse adjustment knob (Page 11)
Light path selector knob (Page 15)
Reflected light mercury lamp housing
(See the separately provided instruction
manual.)
Power supply unit
(See the separately
provided instruction
manual.)
Power supply unit
(See the separately provided
instruction manual.)
Coarse adjustment knob (Page 11)
Coarse adjustment knob rotation tension adjustment ring (Page 11)
Fine adjustment knob (Page 11)
Centering knob
(Accommodation position)

5
BX51WI
Filter Turret *Accepts the combination of a
polarizer push ring and a 32 mm
filter. The thickness should be no
more than 6 mm.
Revolving Arm WI-NPA
# Note that the revolving arm can be mounted only before the reflected light illuminator is mounted or before the
transmitted light arm and IX-SVL2 stage are mounted (page 38).
}This revolving arm accepts the U-SLRE, WI-SNPXLU2 or WI-SRE3 revolving nosepiece.
Light shield tube retaining screw
Sliding Revolving Nosepiece U-SLRE
Switching lever
Single-Position Revolving Nosepiece XLU
WI-SNPXLU2
DIC mount hole
(Page 20)
RMS Adapter WI-RMSAD
Indication label
attaching frame
Filter positions
Accepts 32 mm filters
with thickness of no
more than 6 mm.
Polarizer clamping knob (Page 19)
Polarizer rotating dials
(Page 23)
Polarizer positions (Page 19)*
Accepts the 32PO or 32POIR. Slider insertion/removal
stopper (Page 10)
Filter frame reinforcing ring
(Page 38)
Filter slider (Page 10)
IR filter insertion position
Accepts the 32BP775, 32IR900 or a 32 mm
filter with thickness of no more than 5.3 mm.
Revolving nosepiece mount dovetail
Revolving nosepiece clamping screw
Grounding screw hole (M3)
Microscope frame mount dovetail
XLFluor objective mount screw
M 34 mm, pitch 1 mm
UIS2 (UIS) objective
mount screw
XLU objective mount screw
M 25 mm, pitch 0.75 mm
UIS2 (UIS) objective mount screw
Polarizer push ring (Page 10)
Made of white plastic

6
Centering screw
Swinging Revolving Nosepiece WI-SRE3
Swing lever
Long-WD Universal Condenser WI-UCD
Aperture iris diaphragm lever (Page 17)
Long-WD DIC Condenser WI-DICD
Aperture iris diaphragm
lever (Page 17)
DIC mount hole (Page 20)
UIS2 (UIS) objective mount
screws
Turret (4 positions)
Quarter-wave plate clamping
knob
Quarter-wave plate rotation
ring (Page 23)
Optical element index
mount (Page 21) DIC prism replacement cover
Quarter-wave plate
clamping knob
Quarter-wave plate rotation
ring (Page 23)
DIC prism (large) mount position
DIC prism (small) adapter
Confocality correction washers
(Kinds of thickness: 10, 30 and
50 μm; three washers per kind)
Centering knob

7
BX51WI
Differential Interference Contrast Prisms (For Condenser)
}The WI-UCD condenser accepts two large and two small DIC prisms while the WI-DICD condenser accepts one large or
small DIC prism.
When selecting the brightfield (BF) light path using the WI-UCD, leave one DIC prism (large) mount position empty.
· WI-DIC10HR
· WI-DIC20HR
Long-WD Oblique Condenser WI-OBCD
Aperture iris diaphragm lever (Page 17)
High-Resolution DIC Prism A WI-DICTHRA2
DIC Prism WI-DICT2
}This prism can be mounted in the DIC prism position
of the WI-SNPXLU2 or WI-SRE3.
Applicable condensers
WI-DICTHRA2: WI-UCD, WI-DICD
WI-DICT2: U-UCD8
Positioning pin
Condensers and Applicable Objective Magnifications
Condenser Applicable Objective Magnification
WI-UCD
WI-DICD
WI-OBCD
5X or more
U-UCD8
U-SC3 2X or more
U-AAC 10X or more
U-AC2 5X or more
· WI-DIC40HR
· WI-DIC60HR
· U-LDPXLU20HR
Index
Positioning indices
Index
Oblique iris insertion/removal knob (Page 17)

8
(Controls Used) (Page)
TRANSMITTED LIGHT BRIGHTFIELD
OBSERVATION PROCEDURE
}The following flow shows the operating procedure for the transmitted light brightfield observation which is the basic obser-
vation method of this microscope. The operating procedures for DIC observation, fluorescence DIC observation and IR DIC
observation will be described separately in Chapter 5, “OTHER OBSERVATION METHODS” on page 19.
Set the main switch to “ I” (ON). @Main switch (P. 10)
²Lamp ON-OFF switch (P. 10)
(Only when the hand switch is used)
Disengage the filter from the light
path. ³Filter turret (P. 10)
Select the binocular light path. |Light path selector knob (P. 15)
Place the specimen on the stage. ƒX-axis and Y-axis knobs (P. 12)
Engage a low-magnification objective in the
light path. …Swing lever (P. 13)
Bring the specimen in focus. †Coarse/fine adjustment knobs (P. 11)
Adjust the brightness. ‡Light intensity control knob (P. 10)
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
ŠBinocular tube (P. 14)
‰Diopter adjustment ring (P. 14)
‹Condenser height adjustment knob (P. 16)
ŒCondenser centering knob (P. 16)
Adjust the aperture iris and field iris diaphragms. ™Aperture iris diaphragm lever (P. 17)
šField iris diaphragm ring (P. 16)
Engage the objective to be used in the light
path and bring the specimen in focus.
…Swing lever (P. 13)
†Coarse/fine adjustment knobs (P. 11)
Engage the required filters. ³Filter turret (P. 10)
Adjust the brightness. ‡Light intensity control knob (P. 10)
Start observation.
Tip for microscope operation
In patch-clamp testing, switch the microscope controls cautiously and gently so that the patch
electrodes do not slip off.

9
BX51WI
|
} Make a photocopy of the observation procedure pages and post it near your microscope.
‰
Š
…
ƒ
‹Œ™
³
†
š
†
†
@
‡
²
‡
Used only when the hand
switch is not connected

10
Fig. 2
Fig. 3
Fig. 4
USING THE CONTROLS
4-1 Microscope Base, Power Supply Unit (TH4)
1Controlling the Light Intensity (TH4) (Figs. 2 & 3)
}See the separately provided instruction manual for details.
1. Make sure that the light intensity control knob @ is set to MIN (minimum
voltage), then set the main switch ² to “ I” (ON). (The POWER LED ³
should light up.)
2. Turn the knob @ clockwise toward MAX (maximum voltage) to increase
the intensity and brightness.
}The marking indicates the position where the optimum daylight for
color photography is obtained when the LBD filter is engaged in the light
path.
Operation Using the Hand Switch
}When the hand switch is connected (when the REMOTE LED | is lit), the
light intensity control knob @ is defeated and only the light intensity
control knob ƒ of the hand switch can be used.
The hand switch is provided with double-side adhesive tape so that it
can be attached onto a convenient position for operation.
1. After setting the main switch ²to “ I” (ON), press the lamp ON-OFF switch
… to ON and adjust the brightness with the intensity control knob ƒ.
2. To turn the lamp OFF, press the lap ON/OFF switch … again to OFF.
#The lighting of the REMOTE LED | indicates that the hand switch is
standing by. The hand switch consumes a power of about 2.5 W
when it stands by.
When the system is not to be used for a lone period, be sure to set
the main switch ² to “ ” (OFF).
2Using the Filter Turret (Fig. 4)
}Filters with a diameter of 32 mm can be inserted in positions @ to |.
1. Filter positions @ and ² are rotatable. When the 32PO polarizer or 32POIR
polarizer is placed in either position, the polarizer or filter can be fixed by
using the push ring (made of white plastic).
}When filter position @ is engaged in the light path, the rotation fixing
knob ƒ comes at the front where the operation is easy.
2. Filter position ³ accepts any type of 32 mm filter.
#When using two filters together, the thickness of the lower filter should
be no more than 2 mm. Otherwise, the upper filter may drop during
rotation.
3. Filter position | accepts the 32BP775 or 32IR900 filter. As the filter can-
not be inserted unless the filter slider is removed, remove it by releasing
the insertion/removal stopper below the slider and loosening the slider
clamping screw using the provided Allen screwdriver.
@
²
³
|
ƒ
…
@
²
³
|
ƒ

11
BX51WI
Fig. 5
Fig. 6
Fig. 7
4-2 Focusing Block
1Using the Pre-focusing Lever (Fig. 5)
}The pre-focusing lever prevents collision between the specimen and
objective and simplifies the focusing operation. After bringing the
specimen into approximate focus with the coarse adjustment knob, turn
the pre-focusing lever @ in the direction of the arrow to lock it. Hereafter,
the lower limit of the coarse adjustment will be limited at the position
where the lever is locked. When bringing a specimen in focus, approximate
focus can be obtained by simply lowering the coarse adjustment to the
stopper position so all you have to do more is control the fine adjustment
knob.
}The up/down movement using the fine adjustment knob is not limited.
# When the pre-focusing lever is locked, the coarse adjustment stroke
is limited by the mechanism and it cannot reach the previous upper
limit. If you want to control the coarse adjustment knob to the previous
upper limit, unlock the pre-focusing lever.
2Using the Fine Adjustment Fast-Feed Knob (Fig. 6)
}Fine focusing is usually possible while the rubber cap @ is attached.
However, when it is desirable to allow the fine adjustment knob to vary
focusing by a large amount, though this is not be as large as with the
coarse adjustment knob, the rubber cap can be removed and the pro-
vided fine adjustment fast-feed knob attached in place.
}If you remove the knob by loosening the screw clamping the fine adjust-
ment knob ³ from the opposite side, the fine adjustment can be con-
trolled using the tip or thick of your finger.
3Using the Frost Switching Lever (Fig. 7)
}Low observation light can be brightened by turning the frost switching
lever @ which controls the built-in frost filter, in the direction of the arrow.
However, although the brightness is increased, irregularity in lighting may
also increase.
4 Adjusting the Coarse Adjustment Knob Rotation Tension
(Fig. 5)
# Do not adjust the coarse adjustment knob rotation tension adjust-
ment ring (² in Fig. 5) because the belt interlocking of the ring with
the coarse adjustment knob on the front has been adjusted at the
factory. If the tension is varied, the accuracy of the pre-focusing lever
will deteriorate.
@
²
@
²
@
³

12
Fig. 8
Fig. 9
Fig. 10
Fig. 11
4-3 Stage (IX-SVL2)
1Placing the Specimen (Fig. 8)
1. Place the specimen on the center of the stage.
}The optional stage center plate (IX-CP50) makes it possible to observe a
wide range of a big petri dish, etc. (Central hole diameter: 50 mm)
2Moving the Specimen (Fig. 9)
1. The specimen can be moved by turning the X-axis knob @ and Y-axis
knob ².
The movement strokes are 50 mm (X-axis) x 43 mm (Y-axis).
3Setting the Grounding (Fig. 10)
}In case of electrical physiological experiment, etc., the specimen can be
grounded from the stage.
Prepare a grounding wire @ and M4 screw ² and attach grounding as
shown in Fig. 10.
# The screw hole may sometimes be stuck by paint, etc. In such a case,
screw in the M4 screw a few times to expose the metallic thread
inside the screw hole and improve the contact before attaching the
grounding wire firmly.
4 Adjusting the X-Axis/Y-Axis Knob Rotation Tension
(Fig. 11)
}The rotation tension of the X-axis and Y-axis knobs can be adjusted
independently.
1. Loosen the 2 set screws @ of a knob using the provided Allen wrench,
hold the stage so that it will not move, then turn the knob to adjust the
tension. Turning it in the direction of the arrow increases the tension and
turning in the opposite direction decreases the tension.
2. After adjustment, tighten the set screws firmly.
# If the tension of a knob is too heavy or too light, skipping or returning of
image may occur during the stage movement.
@
²
²
@
@

13
BX51WI
Fig. 12
Fig. 13
Fig. 14
5Using the Light Shield Sheet (Fig. 12)
# The light shield sheet provided with the reflected light fluorescence
illuminator is too small to be used with the BX51WI. Always use the
light shield sheet provided with the BX51WI microscope frame.
}During fluorescence observation using a low-magnification objective, the
fluorescence image may be deteriorated due to light reflected from the
condenser or the surroundings. In this case, use the light shield sheet.
1. Lower the condenser to the lower limit position using the condenser
height adjustment knob.
2. Insert the light shied sheet all the way into the gap between the upper
and lower stages on the side of the stage (IX-SVL2).
# If the condenser is lowered insufficiently, the sheet cannot be in-
serted into the normal position and the light shielding effect cannot
be obtained.
6Lowering the Stage Height
}The stage can be lowered by 50 mm by removing the condenser holder.
See page 42 for details.
4-4 Revolving Nosepiece
If the petri dish in use is filled with liquid, it may splash when the objective is switched. As such liquids are sometimes
toxic, be sure to move the revolving nosepiece away from the petri dish before switching the objective.
Even after the revolving nosepiece has been moved, re-focusing is easy by making use of the pre-focusing lever (page 11).
1 Switching the Objectives (U-SLRE, WI-SRE3)
(Figs. 13 & 14)
}The clicking force of the revolving nosepiece has been set weak in order
to reduce vibrations during objective switching.
To reproduce the correct click position, switch the objectives gently by
operating the lever.
Sliding Revolving Nosepiece U-SLRE
Switch the objective by holding the objective switching lever @ and
gently moving it back and forth.
}By attaching the objective switching lever @ on the opposite side, a UIS
objective can be positioned on the front side of the microscope.
Sliding Revolving Nosepiece WI-SRE3
Switch objectives by gently puling up or pushing down the swing
lever @.
Pull up or push down the swing lever gently until it hits the revolving
nosepiece’s stopper.
@
@
@

14
Fig. 15
Fig. 16
Fig. 17
Fig. 18
4-5 Observation Tube
1Adjusting the Interpupillary Distance (Fig. 15)
While looking through the eyepieces, adjust for binocular vision until the
left and right fields of view coincide completely. The index dot · indicates
the interpupillary distance.
}Note your interpupillary distance so that it can be quickly duplicated.
2Adjusting the Diopter (Figs. 16 & 17)
1. Looking through the eyepiece without the diopter adjustment ring, rotate
the coarse and fine adjustment knobs to bring the specimen into focus.
2. Looking through the eyepiece sleeve with the diopter adjustment ring @,
turn only the ring to focus on the specimen. (Fig. 16)
Using a Finder Eyepiece
1. Looking through the right eyepiece with your right eye, turn the top of the
eyepiece ² until a clearly defined double crosslines can be seen in the
field of view. (Figs. 16 & 17)
2. Looking through the right eyepiece, rotate the coarse and fine adjust-
ment knobs to bring the specimen and double crosslines into simulta-
neous focus.
3. Looking through the left eyepiece with your left eye, turn the diopter
adjustment ring @ to focus on the specimen.
3Using the Eye Shades (Fig. 18)
When Wearing Eyeglasses
Use with the eye shades in the normal, folded-down position. This will
prevent the eyeglasses from being scratched.
When Not Wearing Eyeglasses
Extend the folded eye shades in the direction of the arrow to prevent
extraneous light from entering between the eyepieces and eyes.
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15
BX51WI
Fig. 19
Fig. 20
4Using Eyepiece Micrometer Disks (Fig. 19)
Eyepiece micrometer disks can be inserted into the WHN10X-H (or
WHN10X) eyepieces.
However, if the eyepiece does not have the helicoid adjustment facility
and your eyesight is poor, you may have difficulties in focusing on the
eyepiece micrometer disk. In this case, it is recommended to look into
the eyepiece through your eyeglasses.
Use 24 mm dia. x 1.5 mm micrometer disks.
Following Fig. 19, remove the micrometer mounting frame ² from the
eyepiece and place a micrometer disk @ into the mounting frame.
Re-attach the micrometer mounting frame in the original position.
5 Selecting the Light Path of Trinocular Tube
(Fig. 20)
Slide the light path selector knob @ to select the desired light path.
Trinocular
Tube
Light Path Selector Position
Pushed In Intermediate Pulled Out
U-TR30-2 Binocular 100% TV, photo 100%
U-ETR3 Binocular 100% TV, photo 100%
U-TR30IR Binocular 100% Shutter TV, photo 100%
Binocular 20%,
TV, photo 80%
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16
Fig. 21
Fig. 22
4-6 Condenser
1Centering the Condenser (Figs. 21 & 22)
1. Set the aperture iris diaphragm lever @ to the open position. (Fig. 22)
2. Set the field iris diaphragm ring ² to the open position ( ¦). (Fig. 21)
3. Focus on the specimen using the 10X objective.
4. Close the field iris diaphragm ring ² so that the diaphragm image comes
inside the field of view.
5. Manipulate the condenser height adjustment knob ³ to focus on the
diaphragm image.
6. While opening the field iris diaphragm gradually, turn the two condenser
centering screws | on the condenser holder to move the iris diaphragm
image to the center of the field of view. (Fig. 21, Fig. A Fig. B).
7. Gradually open the field iris diaphragm. The condenser is properly cen-
tered if the iris image is centered and inscribed in the field of view (Fig. B
Fig. C).
}During actual use, open the field diaphragm slightly until its image cir-
cumscribes the field of view.
Fig. A Fig. B Fig. C
Field Iris Diaphragm
The field iris diaphragm restricts the diameter of the beam of light enter-
ing the objective and thus excludes extraneous light, improving image
contrast. The diameter of the field iris should be adjusted for objective
magnification to the extent that it just circumscribes the field of view.
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