Olympus FV3000 User manual

FV3000
Operation Manual

Contents
-----------------Image Acquisition---------------
Starting the System ――――――――――――――――― 4
Visual observation with microscope ―――――――――― 5
XY Image Acquisition ――――――――――――――――― 6
XYZ Image Acquisition ―――――――――――――――― 8
XYT Image Acquisition ―――――――――――――――― 9
4D（XYZT）Image Acquisition ―――――――――――― 10
XYT Image Acquisition（with Z drift compensation） ―― 11
Multi Area Time Lapse ―――――――――――――――― 12
Multi Area Time Lapse using Map Image（１）――――― 14
Tips of Image Acquisition ――――――――――――――― 17
Exiting the system ―――――――――――――――――― 18
Configuration ―――――――――――――――――――― 19
------------------Processing Image ----------------
2D view and operation ――――――――――――――― 21
3D view and operation ――――――――――――――― 22
Creating the movies ――――――――――――――――― 23
Processing: Projection ―――――――――――――――― 24
Exporting the image ―――――――――――――――― 25
Reloading and saving Observation Methods ―――――― 26
------------------Spectral Imaging------------------
Lambda Series（acquiring by single channels）―――― 28
Lambda Series（acquiring by multiple channels）――― 30
Processing: Unmixing（１） ――――――――――――― 32
Processing: Unmixing（２） ――――――――――――― 33
Processing: Unmixing（３） ――――――――――――― 34

Image Acquisition

Starting the system
Turning ON the power
① Set the main switch of the PC to ON
② Switch on the central power..
Laser controller
③ Switch on the main power of each
laser. And Rotate the start key.
Touch Panel Controller（TPC)
④ Press the main switch of the touch
panel controller to set it to ON.
Mercury burner for the light source
⑤ Press the lamp switch on the front side
of the light source.
Logon Windows
⑥ Logon Windows with your own user ID
and password.
Starting software for FV3000
⑦ Double-click the [FV31S-SW] icon on
the desktop.
※Start the software after “Start operation”
is displayed in TPC.
⑦ The stage setting dialog box appears.
Set each checkbox and click [OK].
4
Factory Default ID & password
Factory User ID：olympus
Password：olympus
Switch on the
main power
Rotate
start key
③Laser Combiner
TPC main
switch
④Touch Panel Controller（TPC)
ON/OFF
switch
To be blue
⑤Mercury burner
②Central
power
①PC
switch
Selection whether or not to initialize the
resonant scanner
XY stage type
Selection whether or not to control the XY
stage by the software
Selection whether or not to perform the
centering in XY direction

Visual observation with microscope
★Fluorescence observation
*Tap the button of the TPC to select
the objective for observation.
① Tap the light path selection button
of the touch panel controller.
(or select “Ocular” in [Ocular] Tool
Window. )
② Tap the button.
③ Tap the [EPI(or EPI1) tab.
④ Select the mirror unit suitable for the
fluorescent probe for observation.
⑤ Open the shutter of the reflected
illumination light on the touch panel
controller.
⑥ Select the ND filters to adjust the
brightness.
※Brightness can be adjusted by rotating
the light volume adjustment dial of
the light source.
Rotate the light volume
adjustment dial
⑦ Adjust the focus
using the focus
knob.
★Transmitted DIC observation
*Tap the button on the TPC to
select the objective for observation.
① Tap the light path selection button
of the touch panel controller.
② Tap the button.
③ Tap the [DIA] tab.
④ Tap the [Elements] tab
and check whether
the [Polarizer] is “IN”.
⑤ Insert the DIC prism
slider.
⑥ Open the shutter of the transmission
illumination light on the touch panel
controller.
⑦ If necessary, tap the [Brightness] tab
and adjust the brightness.
⑧ Adjust the focus and contrast.
④
①
②
⑤
④
③
⑥
3
①
②
⑦
③
⑥
Adjust the DIC contrast
by rotating the knob.

XY Image Acquisition(1)
① Select “VBF” in the [PMT Setting] Tool Window.
Assigning the detector to channel
② Press the button on [PMT Setting]
Tool Window to open the [Dye & Detector Select]
dialog box.
③ Press the button to reset the Assigned
Dye.
④ Double-click the name of the fluorescence dye to
observe.
⑤ When registering a fluorescence dye, TD channel
is registered automatically. If you don’t need,
double-click the TD channel to cancel it.
⑥ If you want to observe with multiple phases using
the virtual channel scan, press the button
to add the phase. And drag&drop the selected
“Dye” to the observation channel list of the phase
you want to add.
⑦ After setting all channels, press the [OK] button.
Adjusting the live image
⑧ If you want to acquire multiple channel image,
select Sequential scan “Line” in the [PMT Setting]
Tool Window.
⑨ Press the button on the [Live] window.
Adjust focus and set Laser Intensity(%),
Sensitivity(HV), Gain and Offset on [PMT setting]
Tool Window.
⑩ If you use virtual channel scan, press
to switch phases. On both phases, adjust focus
and set Laser Intensity(%), Sensitivity(HV), Gain
and Offset.
Virtual Channel Scan
This mode allows to acquire multiple CH image
whose channel number is larger than the
number of detector.
①
Adjusting Confocal
Aperture.
Reducing noises by
Averaging.
②
③
④
⑤
⑦
⑧
⑨
Laser Intensity
Sensitivity
4
⑩
【Hi-Lo Mode】
pixels of intensity 4095 are shown in red.
Pixels of intensity 0 are shown in red.
u5

Slide 6
u5 確認（英語のほう）
元島 ゆき; 03.02.2017

XY Image Acquisition(２)
Setting the scanner
⑩ On [LSM Imaging] Tool Window, select the
scan method in [Type] and [Mode].
※See 23 pages for details.
⑪ Set scanning speed.
⑫ Specify “Image Size” to set the scan area.
⑬ Set the zoom, rotation and clip scan if
needed.
Starting Acquisition
⑭Select [Normal] tab in [Acquire] Tool Window.
Press the button to open the dialog
box, and select the folder to save the images.
*The acquired images are saved automatically.
Serial number is added at the end of file name
like “***_0001” and “***_0002”.
⑮Press button to start acquiring the
image.
5
DIC observation
1. Insert the DIC slider to microscope.
2. Register the TD channel.
3. Set the laser to be used for observation.
4. Adjust the sensitivity(HV).
5. Adjust the contrast by rotating the knob of
the DIC slider.
(See page 3 ⑤ in left side. )
Attention
Check [LSM] in [Series] Tool Window whether it
is selected “OFF” [Z] and [Time] .
⑩
⑪
⑫
⑬
⑮
⑭
4.
3.

XYZ Image Acquisition
*Before starting the following procedure, make
adjustments for XY imaging.
(refer to page 4-5.)
Setting Z series
① Select “ON” in [Z] on [Series] Tool Window.
② Select “Start/End” in [Motor] on [Z section].
Register start/end position
③ Change the Z position by rotating the
focusing knob or pressing the
button. Press the button in [Start]
at the Z position to start acquiring the
image.
④ Change the Z position and go on to press
the button in [End] at the Z position
to end acquiring the image.
⑤ Enter a value in [Slices] or [Step Size].
Setting one will set other automatically.
Pressing the button, both numerical
values “Slices” and “Step Size” is optimized.
Starting Acquisition
⑥ Select [Normal] tab in [Acquire] Tool
Window. Press the button to display the
dialog box, and select the folder to save the
images.
Press the button to start acquiring
the image.
Finishing Acquisition
⑧ Finishing acquisition, buttons blink.
Press the button to complete the
image acquisition. If you want to Additional
images under the same condition, enter the
number of additional acquisition and press
the button. After the image is
acquired, press the button.
①
③
⑤
②
④
⑥
⑦
⑧
6
:Each click shifts the position by the value
entered for Step Size
:Each click shifts the position by ½ the value
entered for Step Size.
Pressing the button, current
position is registered as origin.
u6
u7

Slide 8
u6 新しく追加。要チェック。
元島 ゆき; 08.02.2017
u7
Step Size
にしたけど大丈夫だろうか？
内容・英語ともに。
元島 ゆき; 08.02.2017