Olympus BX46 User manual

INSTRUCTIONS
BX46
CLINICAL MICROSCOPE
This instruction manual is for the Olympus Clinical Microscope Model BX46.
To ensure the safety, obtain optimum performance and to familiarize yourself fully with the use
of this microscope, we recommend that you study this manual thoroughly before operating the
microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
A X 7 8 5 3

In accordance with European Directive 2002/96/EC on Waste Electrical and Electronic Equipment,
this symbol indicates that the product must not be disposed of as unsorted municipal waste, but
should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in your
country.
NOTE: This equipment has been tested and found to comply with the limits for a Class A digital device,
pursuant to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection
against harmful interference when the equipment is operated in a commercial environment. This
equipment generates, uses, and can radiate radio frequency energy and, if not installed and used in
accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case
the user will be required to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance
could void the user's authority to operate the equipment.

BX46
CONTENTS
Correct assembly and adjustments are critical for the microscope to exhibit its full performance. If you are going to assemble
the microscope yourself, please read section 10, “ASSEMBLY" (pages 31 to 34) carefully.
IMPORTANT -- Be sure to read this section for safe use of the equipment. -- 1-3
13-23
4
5-7
8,9
10-12
1 MODULE NOMENCLATURE
2 CONTROLS
3 FLOW OF OBSERVATION
4 SIMPLIFIED OBSERVATION PROCEDURE
4-1 Basic Operation (Until Observation of Specimen)........................................................................10,11
4-2 Microscope Adjustments (How to Improve the Observed Image)........................11,12
1Adjusting the Interpupillary
Distance.................................................................................11
2Adjusting the Diopter..........................................11
3Adjusting the Centering..................................12 4Adjusting the Contrast......................................12
5 USING THE CONTROLS
5-1 Base.................................................................................................................................................................................................................13,14
1Adjusting the Brightness...............................13 2Setting the LIM Brightness..........................13
3Using the Filters..........................................................14
5-2 Focusing Block................................................................................................................................................................................15,16
1Focusing Controls.................................................. 15 2Adjusting the Focus............................................. 15
3Replacing the Fine Adjustment
Knob............................................................................................16
4Adjusting the Coarse Adjustment
Knob Tension..................................................................16
5Pre-focusing Lever..................................................16
5-3 Stage................................................................................................................................................................................................................17,18
1Placing the Specimen.......................................17 2Adjusting the X- and Y-Axis Knob
Tension................................................................................... 18
3Rotating the Stage................................................. 18
5-4 Observation Tube.................................................................................................................................................................... 19-22
1Adjusting the Diopter..........................................19 2Using the Eye Shades.......................................19
3Using the Eyepiece Micrometer
Disk............................................................................................ 20
4Selecting the Light Path of the
Trinocular Tube......................................................... 20
5Adjusting the Tilt..................................................... 21 6Using Eyepieces Incorporating a
Micrometer...................................................................... 22
5-5 Condenser................................................................................................................................................................................................... 22
5-6 Immersion Objectives................................................................................................................................................................. 23

6 CAMERA RECORDING
7 TROUBLESHOOTING GUIDE
8 SPECIFICATIONS
9 OPTICAL CHARACTERISTICS (UIS2 Series)
10 ASSEMBLY -- See this section for the replacement of the light bulb. --
11 HALOGEN LAMP SOCKET INSPECTION SHEET
24
25-27
28,29
30
31-34
35
nPROPER SELECTION OF THE POWER SUPPLY CORD.......................................................... 36,37

1
BX46
SAFETY PRECAUTIONS (Fig. 1)
IMPORTANT
1. If potentially infectious samples may be observed, use protective gloves or
other protective means to prevent the skin from contacting with samples
directly.
After observation, be sure to clean the portion contacted with samples.
· Moving this product is accompanied with the risk of dropping the
samples. Be sure to remove the samples before moving this product.
· In case the samples is damaged by erroneous operation, promptly
take the infection prevention measures.
· Follow the procedures described in Chapter “Getting Ready” (see on
page 2) prior to using the accessories of this product. Otherwise, the
stability of the device will be lost and the dropped samples will cause
the possibility of infection.
· When you maintain the device which may have contacted with
potentially infectious reagents, be sure to wear the protectors such as
gloves, or clean the device prior to operation.
· Before disposing of device contacted with potentially infectious samples,
be sure to follow the regulations and rules of your local government.
2. Install the microscope on a sturdy, level table or bench.
3. Be careful not to tilt the microscope too much. Otherwise, the mobile
parts such as the light path selector knob may move in unintended
directions.
4. Always use the power cord provided by Olympus. If no power cord is
provided, please select the proper power cord by referring to the section
“PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of
this instruction manual.
If the proper power cord is not used, product safety performance cannot
be warranted.
5. Always ensure that the grounding terminal of the microscope and that of
the wall outlet are properly connected. If the equipment is not grounded,
Olympus can no longer warrant the electrical safety performance of the
equipment.
Fig. 1
Operation Using the LED Lamp
Safety Note on LED (Light Emitting Diode)
The LED incorporated in the LED lamp housing is a class 1 LED
product. The light emitted by LED is basically safe, but do not look at
the illumination light directly for an extended period to prevent your eye
from being injured
CLASS 1 LED PRODUCT
Operation Using the Halogen Bulb
1. To avoid potential shock hazards and burns when replacing the halogen
bulb, set the main switch of the TL4 power supply unit to “ ” (OFF) and
allow the lamp socket @ and bulb to cool before touching them.
Designated bulb 6V30WHAL (PHILIPS 5761)
Always use the designated lamp bulb. Using other bulb may
cause malfunction.
2. If the lamp cable or power cord comes in contact with the lamp socket
or its surroundings, the cable or cord may be melted by the heat, causing
an electric shock or fire hazard. To prevent this, distribute the lamp cable
and power cord at enough distances from the lamp socket.
3. The standard service life of the lamp socket is eight (8) years of use or
20,000 hours of total power ON period, whichever is the shorter period.
For details, see Inspection Sheet on page 35.
1
CAUTION

2
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol Explanation
Indicates that the surface becomes hot, and should not be touched with bare hands.
Indicates a non-specific general hazard. Follow the description given after this symbol or in
instruction manual.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
Caution engraving/label
Caution engraving and label are placed at parts where special precaution is required when handling and using the
microscope. Always heed the cautions.
Caution engraving
position Halogen lamp socket [Caution against high temperature]
Caution label
position Rear panel [Caution against high temperature]
If a caution engraving or label is dirty or peeled off, contact Olympus for the replacement or other inquiry.
1Getting Ready (Fig. 2)
1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact. Also note that the microscope
does not have a waterproof construction.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, dust or vibrations. (For the operating conditions,
refer to Chapter 8, “SPECIFICATIONS”.)
3. When moving the microscope, remove the observation and specimen
and carefully carry the microscope by holding the grasping part on the
rear of the arm and the front part of the base as shown in Fig. 2.
To prevent damage to the microscope, never hold it by the stage
or coarse/fine adjustment knob.
· Even when moving the microscope along the desktop surface, be sure
to remove the specimen in advance.
· When the microscope needs to be packaged for forwarding to a remote
location, contact Olympus.
4. When the accessories of this product are used, make sure that the system
height does not exceed 1 meter. Otherwise, the stability of the device will
be lost.
5. When an arm, illuminator, intermediate attachment, observation tube and
camera are to be attached to the upper part of the microscope frame,
make sure that their total weight does not exceed 7 kg.
Fig. 2
CAUTION
Restrictions in Use
When all combinations (@ to ³) below are satisfied at the same time, the microscope may fall if tilted larger than a certain
degree, comparing to other combinations. Please mount the camera port of the intermediate observation tube toward
diagonally backward 45° to prevent from falling.
@The observation tube is either the trinocular tube, U-TTBI, U-ETBI or U-TTLBI.
²The intermediate observation tube is either U-TRUS or U-DP.
³The camera heavier than 0.4kg is mounted on the intermediate observation tube described in ².

3
BX46
2Maintenance and Storage
1. To clean the lenses and other glass components, simply blow dirt away using a commercially available blower and wipe
gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available absolute
alcohol.
· Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks --- for example, electrical
equipment that is being switched on or off, which could cause ignition of a fire.
Also remember to always use it only in a well-ventilated room.
· Set the main switch to “ ” (OFF) and wait for the lamp housing to cool down sufficiently before proceeding
to maintenance.
2. Do not use organic solvents to clean the microscope components other than the glass components. To clean them, use
a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
3. Do not disassemble any part of the microscope except for the parts that are specified to be disassembled in this manual,
as this could result in malfunction or reduced performance.
4. After using the microscope, set the main switch to “ ” (OFF), (wait for the lamp socket to cool down sufficiently if the
halogen bulb has been used,) and keep it covered with a dust cover during storage.
5. Before disposing of this product, be sure to follow the regulations and rules of your local government.
3Caution
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
CAUTION
The following symbols are used to set off text in this instruction manual.
: Indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury or damage to the equipment or other property. It may also be used
to alert against unsafe practices.
} : Indicates commentary (for ease of operation and maintenance).
CAUTION
4Intended use
This product has been designed to be used to observe magnified images of specimens in various routine work and
research applications.
Do not use this product for any purpose other than its intended use.
This product is considered a medical device in the following country: US.
This product complies with the requirements of directive 98/79/EC concerning in vitro diagnostic
medical devices. CE marking means the conformity to the directive.
Safety and EMC Precautions
· This product complies with the emission and immunity requirements described in IEC61326 series.
· The electromagnetic environment should be evaluated prior to operation of this product. Do not use this product in
close proximity to the sources of strong electromagnetic radiation to prevent interference with the proper operation.
· Use only power cord which OLYMPUS specifies. Otherwise the safety and EMC performance of the product can not
be assured.
· Be sure to ground the product. Otherwise our intended electric safety and EMC performance of the product can not
be assured.

4
MODULE NOMENCLATURE
1
}The modules mentioned below show only the typical product names. As there are some products that are not mentioned but
also applicable to this microscope, check the latest catalogues or consult Olympus.
For the products marked “ *”, also read their instruction manuals.
Eyepieces
· WHN10X
· WHN10X-H
Slide holder
· U-HLST-4
· U-HLS-4
Stage
· U-SVRC
· U-SVRC-CY
Observation tube
· U-TTLBI
· U-TBI-3
· U-BI30-2
Intermediate attachment
· U-TRU
· U-CA
· U-DO3
Lamp housing
· U-LH100-3
· U-LS30ADP +
U-LS30-5 + TL4*

5
BX46
CONTROLS
2
2
· If you have not yet assembled the microscope, read section 10, “ASSEMBLY” (pages 31 to 34).
Interpupillary distance
adjustment scale (Page 11)
Diopter adjustment ring
(Page 19)
Slide holder (Page 17)
Aperture iris diaphragm ring
(Page 12)
Field iris diaphragm ring
(Page 12)
Allen screwdriver holder
Y-axis knob (Page 18)
X-axis knob (Page 18)
Coarse adjustment knob
(Page 15)
Coarse adjustment tension
adjustment ring (Page 16)
Filter slider insertion slot

6
Main switch (Page 10)
I : ON
: OFF
Fine adjustment knob
(Page 15)
Detachable.
Pre-focusing lever
(Page 16)
LIM indicator
ON : Lit (Green)
OFF : Extinguished
LIM ON-OFF switch (Page 13)
LED brightness adjustment
knob (Page 13)
Condenser height
adjustment knob
(Page 12)
Top lens swing-out
lever (Page 12)
LIM setting switch (Page 13)

7
BX46
<< Modules for halogen lamp operation >>
LS30 Adapter
U-LS30ADP
Guide pin holes
Halogen Lamp Socket
U-LS30-5
Guide pins
Power Supply Unit
TL4
Lamp cable
Brightness control
knob
Main switch
I: ON. : OFF.
Lamp socket connector

8
3
3FLOW OF OBSERVATION
}When the LED lamp is used and the LIM is set, the LED brightness adjustment knob is defeated.
}When the halogen bulb is used, set the 32LBD filter in the filter slider and insert it into the insertion slot @.
Set the main switch to “ I” (ON) and adjust
the brightness.
Select the light path (trinocular tube).
Place the specimen on the stage.
Engage the 10X objective in the light path.
Bring the specimen in focus.
Adjust the interpupillary distance.
Adjust the diopter.
Adjust the light axis.
Adjust the aperture iris and field iris diaphragms.
Engage the objective to be used in the light
path and bring the specimen in focus.
Adjust the brightness.
Engage the required filters.
Start observation.
(Controls Used) (Page)
LED lamp Halogen bulb
@ Main switch
2LED brightness
adjustment knob*
2LED brightness
adjustment knob*
3Main switch
4Brightness
adjustment knob
4Brightness
adjustment knob
5Light path selector knob (P. 20)
6Slide holder (P. 17)
7X-/Y-axis knobs (P. 18)
8Revolving nosepiece
9Top lens swing-out lever (P. 12)
aCoarse/fine adjustment knobs (P. 15)
bBinocular tube (P. 11)
cDiopter adjustment ring (P. 19)
dCondenser height adjustment knob (P. 12)
eCondenser centering screws (P. 12)
fAperture iris diaphragm ring (P. 12)
gField iris diaphragm ring (P. 12)
8Revolving nosepiece
9Top lens swing-out lever (P. 12)
hFilter mount (P. 14)
(P. 13)
(P. 13)
(P. 13)
*The LED brightness adjustment knob is defeated when the LIM is set (see page 13).

9
BX46
5(trinocular tube only)
} Copy the observation procedure pages on separate sheets and post it near your microscope.
c
b
8
6
f
e
2
a
9
d
hg
7
1
4
3

10
4SIMPLIFIED OBSERVATION PROCEDURE
4-1 Basic Operation (Until Observation of Specimen)
Fig. 3
1
This section describes the basic operation of the microscope until the start of observation of a specimen. For the detailed
operating procedure of each control, please read the description page specified below.
Press the main switch of the microscope frame to “ I” (ON).
Fig. 4
Fig. 5
2
3
4
3
4
Rotate the knob to adjust the lamp brightness. (Details: Page 13)
Rotate the knob to lower the stage. (Details: Page 16)
Open the clamping lever of the slide holder and place the specimen
slide. (Details: Page 17)
1
2

11
BX46
Fig. 6
5
6
7
Rotate the revolving nosepiece to engage the 10X objective in the
light path.
Rotate the coarse and fine adjustment knobs to bring the specimen
in focus. (Details: Page 19)
Rotate the stage knob to adjust the observation position.
Now you can observe the magnified image of the specimen. To
improve the observation further, read section 4-2, “Microscope
Adjustments” below.
4-2 Microscope Adjustments (How to Improve the Observed Image)
Fig. 7
Fig. 8
1
1
Adjust the microscope as described below to improve the observed image.
1Adjusting the Interpupillary Distance
While looking through the eyepieces, adjust for binocular vision until
the left and right fields of view coincide completely.
}Note your interpupillary distance so that it can be quickly duplica-
ted.
2Adjusting the Diopter
The diopter adjustment ensures parfocality from low to high magni-
fications and makes it possible to avoid defocusing even when the
objective is switched.
For details, see page 19.
5
6
7

12
1
2
3
4
5
6
7
8
9
10
11
Place the specimen.
Rotate the revolving nosepiece to select the 10X objective.
Raise the lever to engage the top lens in the light path.
Rotate the knobs to bring the specimen in focus.
Rotate the knob to raise the condenser to its upper limit.
Rotate the field iris diaphragm ring in the direction of the arrow so that the diaphragm image comes inside the field
of view.
Rotate the knobs to focus on the field iris diaphragm image (A in Fig. 9).
Pull out the two condenser centering screws.
Insert the two condenser centering screws into the condenser centering thread holes and turn the two condenser
centering screws to move the iris diaphragm image to the center of the field of view (B in Fig. 9).
Gradually open the field iris diaphragm until its image is inscribed in the field of view (C in Fig. 9).
Open the field diaphragm slightly until its image circumscribes the field of view slightly (D in Fig. 9).
}With the 100X objective, the field iris diaphragm image cannot be observed unless the iris diaphragm is minimized. With
the 4X objective, maximize the iris diaphragm to observe it.
1
2
1
Fig. 9 Movement of Field Iris Diaphragm Image
A B C D
3Adjusting the Centering
4Adjusting the Contrast
NA scale
Set the condenser aperture iris diaphragm ring so that the condenser NA scale position indicates 70% of the NA of the
objective in use. After this, observe the image and fine adjust the aperture iris diaphragm to the desired condition.
The image of the aperture iris diaphragm can be viewed by removing the eyepieces and looking into the observation
tube. Adjust so that the image looks approximately like that shown in the figure above.
2
Aperture iris
diaphragm image
Objective pupil
70-80%
30-20%
1
2
56 7
8
10 11
9
3
4

13
BX46
5USING THE CONTROLS
5-1 Base
Fig. 10
Fig. 11
Fig. 12
1Adjusting the Brightness (Figs. 10 & 11)
1. When the LED lamp is used for observation, turn the LED brightness
adjustment knob @ clockwise to make illumination brighter.
2. When the halogen bulb is used for observation, adjust the brightness
adjustment knob 2of the TL4 power supply unit.
2Setting the LIM Brightness (Fig. 12)
}LIM stands for Light Intensity Manager. It is effective when the U-LHLEDC
LED lamp housing is used.
}The desired brightness can be set per objective. Once the brightness for
the objectives is set, selecting an objective adjusts the LED brightness
automatically provided that the LIM ON-OFF switch @ is set to “ON” (which
is indicated by the lighting of the LIM indicator 2).
1. Press the LIM ON-OFF switch @ to “OFF” (so that the LIM indicator 2is
extinguished).
2. Engage a low-power objective in the light path and bring the specimen
in focus.
3. Rotate the brightness adjustment knob 3for optimum brightness for
observation.
4. Press the LIM setting switch 4to enter the current brightness.
5. Set the optimum brightness for the mounted objectives by repeating the
above for each of them.
To modify the LIM setting:
Engage the objective you want to set the new LIM setting in the light path,
set the LIM ON-OFF switch to “OFF”, and perform steps 3 and 4 above.
1
2
1
2
3
4

14
Fig. 13
3Using the Filters (Fig. 13)
}One of the filters listed below can be engaged in the light path by inserting
the filter @ in the filter slider 2and engaging the filter slider in the light
path.
Usable Filters Applications
32LND1.5/3/6/12/25/50 For light brightness control, transmittance
1.5/3/6/12/25/50%
32LBD For color temperature conversion
32IF550 For B&W contrast (Green)
}The LBD filter is not required when the LED lamp is used.
What is LBD filter?
The color of the sun at the noon of a clear day is called the daylight
color, which looks white to human eyes. On the other hand, the
halogen bulb used as the light source of this microscope does not
emit the daylight-color light. so the LBD filter is engaged in the light
path to convert the color of halogen bulb light into daylight. In general,
engaging the LBD filter makes it possible to observe specimens in
natural color tones.
1
2

15
BX46
5-2 Focusing Block
Fig. 14
Fig. 15
Fig. 16
1
2
5
1
3
4
}The stage of this microscope is fixed at a low height to facilitate replacement of specimens. Take care not to let your
hand and fingers interfere with the stage when operating the coarse adjustment knob.
1Focusing Controls (Figs. 14 & 15)
The focusing block consists of the controls listed in the following table,
which also describes the functions of the controls.
No. Name Function
1Coarse adjustment
knob
Moves the focus position widely.
2Fine adjustment dial Fine adjusts the focus position.
3Fine adjustment
knob
Fine adjusts the focus position. This knob
can be attached to the fine adjustment
dial on either the left or right side. (When
the microscope is shipped, it is attached
to the right-side dial.)
4Pre-focusing lever Sets the lower limit of the objective
position (to prevent contact between the
specimen and objective).
5Tension adjustment
ring
Adjusts the force required to rotate the
coarse adjustment knob.
2Adjusting the Focus (Fig. 16)
With both the coarse adjustment knob @ and fine adjustment knob 2,
rotating the knob in the direction of the arrow raises the objective (lets
the specimen approach the objective).
1
2

16
Fig. 17
Fig. 19
3Replacing the Fine Adjustment Knob (Fig. 17)
The fine adjustment knob has been attached on the left side at
the factory.
}The fine adjustment knob is designed detachable to prevent interference
with hand during manipulation of the X-and Y-axis knobs.
Usually attach the fine adjustment knob on the opposite side to the X- and
Y-axis knobs.
1. Pull and remove the rubber cap @ from the fine adjustment knob.
2. Using the Allen screwdriver, loosen the clamping screw 2and remove
the fine adjustment knob 3.
3. Remove the seal from the fine adjustment knob screw hole on the other
side and attach the knob by reversing the removal procedure.
4. Attach a provided seal on the screw hole 5of the fine adjustment dial
|, from which the fine adjustment knob has been removed.
}The fine adjustment dial | can be operated with your fingertip or finger
surface at the same time as manipulating the X- and Y-axis knobs.
4 Adjusting the Coarse Adjustment Knob Tension
(Fig. 18)
Adjust the coarse adjustment knob tension using the tension
adjustment ring @.
The coarse adjustment knob tension is preadjusted for easy use. However,
if desired, you can change the tension using the tension adjustment ring
@. Turning the ring in the direction of the arrow increases tension, and
vice versa.
The tension is too low if the stage drops by itself of focus is quickly lost
after adjustment with the fine adjustment knob. In this case, turn the ring
in the direction of the arrow to increase tension.
5Pre-focusing Lever (Fig. 19)
}The pre-focusing lever ensures that the objective does not come in contact
with the specimen and simplifies focusing.
After focusing on the specimen with the coarse adjustment knob, turn this
lever @ in the direction of the arrow and lock; the lower limit on coarse
adjustment movement is set at the locked position.
After changing a specimen, approximate refocusing is easily accomplis-
hed by rotating the coarse adjustment knob to reach the pre-focused
position. After this, all you have to do is make fine adjustments with the
fine adjustment knob.
}The objective’s vertical movement activated by the fine adjustment knob
is not locked.
}When the pre-focusing lever is locked, a coarse movement stroke is
reduced due to the microscope’s mechanism, causing the revolving
nosepiece not to be able to be lowered to the lower limit. To lower the
revolving nosepiece to the lower limit, release the pre-focusing lever.
1
235
4
1
CAUTION
CAUTION
Fig. 18
1