Olympus MIC-D User manual

OLYMPUS '
Quick Start Guide

Learn more about your MIC-D, down-load free software and explore
vast image libraries at the Olympus MIC-D website.
Focus
Control
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Detailed software installation instructions may also be found at the MIC-D Website

Quick Start Guide
In the lab or in the field, the Olympus MI C-D por table digita l microscope
captures still and moving images instantly. This Quick Start Guide will
int roduce you to the MIC-D's i nnovat ive design and spectacular imaging
capabilities, so
yo u'll be up and taking p ictur es in no time.
MIC-D Pa
rts Explained
MIC-D
Software Introduction
Choos ingYour Specimen
MIC-D
Basic Controls
Illumination Technique
Image Capture Window
Still Imagesand Mov
ies
Time Lapse Movies
Sav ingImages
Library Window
- ViewingCaptured Images
Image
Process ingWindow
Line Drawingand Annotation
Image Manipulation and Adjustme
nts
Polarized Light Kit
2-3
4-5
6
7
B-9
10-11
12-13
14
15-1B
19
20
21-22
23

2
Get To Know The M C-D
Lamp Head "-
Light Diffuser~
Stage '\.
Optical Zoom
Control
'\.
Magnification
Indicator
Base
Tilting
Lamp Arm
/
(
Focus Control
/
Light Intensity
Control
/

3
....._ Lamp /;I e a d ~~
The Lamp Head contains an extremely bright white L. E.D. This device is brighter than an
ordinary bulb and uses less power. The Lamp Head rotates slightly to allow fine tuning to
the illumination angle. Make sure th
at it is rotated all the way clockwise for Transmitt ed
Light work (Brightfield) and all the way co unte r clockwise for Re fl ected Li ght work.
....._ Li ght Diffuser __
The Li ght Diffuser can be pushed in and pulled out. Keep the Diffuser a ll the way in
during Transmitted
Li ght Illumination, and a ll the way out for Re fl ected Li ght
Ill umination. Vary as ne eded for Oblique and Darkfield settings.
....._I ilting Lamp Arm
Adjustments to the angle of the L amp Arm are the key to the differ en t illumination
techniques. Use the live image to guide adjustments. The Arm should be set to the
vertical position for Transmitted
Lig ht, to 11 o'clock for Ob lique Ill umination and to about
10:30 f or Darkfield. The arm should be rotated all the way counter clockwise for the
Reflect
ed Li ght Setting.
...... Gliding Stage __
The Gliding Stage is the platform to place specimens or samples for observation. The MIC-
D's Stage glides 5mm in any horizontal direction,
and can be ro tat ed 360 degrees. Use
the Gliding Stage to adjust the specimen position. Reme
mbe r t hat up, down and rotational
movements are inverted.
_ -.Lo oom Control
The MIC-D boasts an all glass optical zoo m l en s to enable continuous magnification
changes. The magnification range is an amazing 22x to 255x.
_ -I 'vlagnification Indicator
Use the Magnification Scale to read the zoom pow er, by aligning the scale markings on
the blue
zoom handle to the spot below. Adjust the Magnification Slide Bar in the Image
C
apture software window to match the setting on the MIC-D, in order to calibrate the
scale marking in the live image window and captured image.
....._ Light Intensity. Control
Li ght intensity (and focus) should be initially set with the zoo m at minimum pow er to
prevent under exposure. The
MI C-D's exposure setti ng will then be auto mat ically
adjusted to maintain consistent brightness in
the live image. For Reflected Li ght
Illumin
at ion turn the Lig ht Int en sity Control to maximum.
....._ F. ocus Control
Rotate this knob to bring specimens and samples into clear focus. The MIC-D's lens can
focus on surfaces up to 5mm above the stage.

4
MIC-D Software
Image Capture
Window
.-
.-
Library
Window
Image Processing
Window
ki l

5
MIC-D Software
The MIC-D Software enables you to view, capture, process and
annotate images in three simple windows.
Io-_Image Capture Window
Capture and save still and moving images viewed in the Live
Image Window with the simple click of a mouse button. You can
view all of your captured images in
the tile bar below the live
window. The scale bar, light intensity and image format may also
be adjusted here.
Io-_ Li bra r. y. Window
The Library Window is where you can view your captured
images as thumbnails or in
full size. Still images may be
displayed side by side for comparison purposes. Moving images
may also viewed here.
Io-_Image P- rocessing Window
Annotation, color and brightness adjustments are performed in
this window. Digital zooming as well as white and black
balancing functions are available for selection.

6
Choose Your 5 ecimen or Sam ~le
With the MIC-D 's inv erted lens design, you can image just about any sample,
regardless of its shape or whether light can pass through it- thanks to the Reflected
Light capacity. Micros cope slide preparations and thin, translucent specimens can
be
viewed in Brightfield ,Oblique or Darkfield . Use Oblique Illumination to
highl ight structural details and Darkfield for low contrast specimens. Oblique
Illumination will shade rises and depressions in your specimen orsample. Darkfield
can be used to emphasize color . Use Refle cted Light for anything large and opaque.
Don't
forget that for field work you can use the MIC-D anywhere you can take a
laptop computer.
Tr an smitt ed
Light
!Brightfield!
Oarkfield
Reflected
Light
Obliq
ue Illumin ation

7
MIC-D Basic Controls
Let's start by getting familiar with the basic hardware controls . Keep an
eye on the
Live Image Window as you manipulate the MIC-D controls .
Make sure that the Light Diffuser is pushed in, the Illumination Arm is in
the vertical position and the lamp head is rotated fully clockwise . Turn
the MIC-D's light source on and increase the light intensity by turning the
control clockwise, about one half as far as it will go . The MIC-D will
automatically make adjustments tothe light intensity after it is initially set.
Turn the Optical Zoom Control to a minimum setting. The Optical Zoom
Control enables the user to physically adjust the position of the
microscope's
lens to obtain magnification powers from 22x to 255x!
Bring the specimen into focus in the Live Image Window by rotating the
Focus Control Knob. Make any final adjustments to the light intensity.
Notice that the Gliding Stage Moves freely in any direction. Moving the
Gliding Stage instead ofthe specimen or sample will allow you to scroll
the image smoothly, even at high magnification . When you have found
an interesting deta il, center it in the Live Image Window and zoom in.
Continue to
make adjustments to the focus as needed.

8
Illumination Techni9 _ue _
There are four different i ll umination techniques that you can use to
view your specimen or sample
Transmitted Li ght
Use thistechnique to viewconventional microscope slidesor especially
thin specimens.
This technique is also known as 'Brightfield'.
1. Rotate the Illumination Arm fully clockwise to the vertical position.
.Rotate the
Lamp Head fully counter clockwise.
3. Push in the Light Diffuser
Obli que and Darkfield Illumination
Use either of these techniques on any transmitted light specimen or
sample to emphasize contrast.
Use Oblique Illumination to highlight
structural detail by shadowing the 'hills' and 'valleys'.
Use Darkfield
to create a black background and to emphasize specimen color.
1. Rotate the Lamp Head counter-clockwise.
2. Pull out the Light Diffuser.Adjustments to the light diff-
user position may be required.
3. Slowly rotate the Illumination Arm counter-clockwise:
-
For Oblique Illumination rotate the arm until the back-
ground just begins to darken. Three dimensional details
should displaya shadowing effect.
-
For Darkfield Illumination rotate the Illum ination Arm
further counter-clockwise. Stop when the background
turns black.
Best results are achieved at high
magnification.