Olympus CHA-P User manual

OLY MPUS POLARIZING MICROSCOPE
I INSTRUCTION MANUAL I
,(
MODEL - I I
OLYMPUS

This Instruction manual has been written fartbs use of the Otympus Polarizing Micro-
mpe Madel CHA-F. It is recommended to read the manual carafully in order to
familierlze youmlf fully with the USB af the mimompeon the polarizing attachment
IMPORTANT
-we tlta following points carefully:
1. Always handle the microscope with the care it deserves, and avoid abrupt motions.
2. Avoid exposure of the microscope to direct sunlight, high temperature and humidity,
dust and vibration.
If the microscope is used in ambient temperature higher than 40'~ (104~~1, it ma
impede its proper function. 3
3. Only use the tension adjustment ring for atbring the tension of the coarse adjustment.
Do not twist the two coarse adjustment knobs in the opposite directions simultane-
ously, which might cause damage.
4. Ascertain that the line voltage selector switch on the base plate is set to conform with
the local mains voltage.
1. Lenses must always i3e kept clean. Fine dust on lens surfaces should be blown or
wiped off by maam of an air blower or a clean brush. Carefully wipe off oil or finger-
prints deposited on the lens sutfaces with gauze moistened with a small amount of
xylem, alcohol or ether.
2. Do not use organic solutlans to wipe the surFaces of various camponants. Plastic
parts, especially, should be cleaned with a neutral devteqent.
3. Never diassemble the microscope for repair.
4. The miwascope should be stored in its container immediately after uw. If this is
not possible, it should be mered wivth a vinyl dust mr.
5. Disconnect the line cord from the AC power source kfore fuse replament.

CONTENTS
.....................................
I . STANDARD EQUIPMENT
It . NOMENCLATURE ..........................................
In . ~EMBLY ..............................................
N . lDENTlFlCATlON AND FUNCTION OF VARIOUS COMPONENTS ........
V. OPERATION ..............................................
1 .
Adiustment of Minimum Line Voltage
2 . lnterpupillary Distance and Diopter Adjustments
......................................
.
3 Polarirer Alignment 8
......................................
. 4 Centering the Stage g
5 . Centering the Objectives
6 . Use of Apenure Irk Diaphragm
.....................................
7. Focusing Adjustment 10
8 . Orthosoopic Observation
9 . Conompic Observation ................................... 11
............................................
Vl . OPTICALDATA 12
........................................
VII . TROUBLESHOOTING 13

I. STANDARD EQUIPMENT
Model
Component
Microscope stand with circular rotatable
CHA-P-F
stage and quadruple nmpiem
Intermediate polarizing attachment AH-PA
Quarter wave plate (retardation 1 47.7.3W
AH-TP147
SenltFw tint plate (retardation Wnyr)
AH-TP530
Polarizing monocular tube (45") CH-PMO
Polarizing binocular tube (30°) BH-PSI
Swing-out polarizing condenser BH-PUC
Haim lamp socket CLSH-B
Halogen bulbs BVIOWHAL
CHA-Pal
1
I
I
1
I
0
1
1
2
1
1
1
0
1
2
t
Objectives (strain-f reel
Evepieces
CHA-Pa1
1
1
1
1
0
1
1
1
2
1
1
1
1
1
2
1
PO 4x
W10X
P040X
AH-WF1OX
AH-Miwo WF 1 QX
Spare fuses (MA for 1110-1 113-12DV or 0.3A for 220-24\11
Vinyl dust cwer

I. NOMENCLATURE
Photo: Model CHA-P-651. Model CHA-P-051 is also available by modification of som
components.
axmatior, tube
Revolving nosepiece
Intermediate pojwiaing
atmhmt
Objective
Microscope stand
Condenser
1

Ill. ASSEMBLY
The picture balow illustrates the sequential procedure of assembly. The numbers indicate
the assembly order of various components. Remove dust caps before mounting components.
Take care to keep all glass surfaces ctean, and avoid watching the surfaces.
Insert tha obbctb 10X into
@ Eyepiece
the fixed aperture of tha nos-
P-.
Clamping screw
Quarter wave plete
Sensitive tint
plate
with the lettEtrs "OLYMPUS'
facing in front of the mi-
Clamping screw
Microscope stand
Condenser
@ To AC outlet
Q
Q
0-0
/ Halogen lamp Socket
Aligning red dots, on con-
denser mount and condensas,
in- the conderuar Into the

IV. IDENTI FlCATlON AND FUNCTION OF VAR lOUS COMPONENTS
Observation rube
clamping screw
P
Main switch
R hmat trimmer screw
After swltchlno on. M nmry,
r-te this wrew wlth a coin until
tb bulb 18 dimly Ilt, with the
sliding cmtrol lrmrr at mlntmum
0mltIon.
\ Grounding terminal
/ Line volw selector switch
Set the swltch to conform with
the 1-1 mains volmp.

Mechanical tube length adjust-
ment rings
Bertrand tens turret ring
W llght path; "OUT" for
remmal of the Barwand lens
I
from the li&t path.
Bertrand lens focusing ring
Stage
centering screw
Automatic pre-fowsing lever
Aperture iris diaphragm ring
Numerial aperture sale grrd-
Swinwut knob for top lam
Codewer M.A. Is 0.9
(when top lms swln$s
out N.A. k 0.251
Film mount
Amwe 45rnmdlm f llters.
Bulb mount
\ Lamp house clamping knob
The lamp how cow Ean twr
opened by pulllw down th
kn&: or cl- by pushing it up
untll it snaps In plaes.
More pushing, escertaln that the
knob is positiQned aa shm in
the picture rlght, markd wilh

Match the line voltage selector switch to Imal mains voltage (se page 5).
Switch on the light source.
Adjust the trimmer screw until the bulb is dimly lit (page 7).
Place a spacimn slide on the stage.
Remove the Bertrand lens and analyzer from the light path.
Coarse focus with the 1 OX objective.
Make interpupillary and diopter adjustments (paga 7).
Center the stage (page 9).
Cenw objectiw other than 10X (page 9).
Swing in the desired objective.
Set the condenser, analyzer and Bertrand lens mrrectly according to your microscopic
purpose (page 1 0 and 11).
Adjust light intensity.
Fine focus.
Adjust aperture Iris diaphragm {page 9).
Adjustment of Illumination System
For biological use, however, remove the analyzer, Bertrand lms and sensitive tint plates.
Microampic
rnethd
Ohosm~ic
oWation
ConmopIa
observation
* Cut off this paae at dotted line and put it on the wall near the microscop for use as a re-
minder of microscopic procadure.
OLYMPUS
Obiective
4X ,WX
20x to i~ax
Bmrand
lens
OUT
IN
Condenmr
top lens
OW
IN

V. OPERATION
1. Adjustmm of Minimum Line Voltage
'
The mhirnurn vol- quired for the light source can be adjusted with the rheostat trim
mer smw a? the microscope bas plate in accordanw with the line voltage and frequency.
The built-in rheostat incorporates a thyristor in itssemiconductor circuit for the following
advantages:
(a)
Extredy fine adjustment of light intensity mn be easily achied.
(b) Flickering of the bulb filament is eliminated and the light inmnsity is stabilized.
(cJ Increased life exp$ctanw of th~ bulb.
For adiustment of the minimum line voltam, ascartain
that the voltage selector switch is E& to inform with
the Iml mains voltaw, and the sliding control leuera
is positioned closest to you (low voltage), and then
activate the main switch Q. If the bulb Is dimly lit,
the secondary voltage is correct. If it is not lit at all,
rotate the rheostat trimmer screw @ gradually with a
win, until the bulb is dimly lit; then push the stiding
control lever forward in order to obtain optimum light
intensity. (Fig. 1)
Fig. 1
2. Interpupiltary Distanm and Diopter Adjmnis
1) Insert lhe evwieae @ with cross hairs into the right
eyepiem tube of the binocular tub, aligning the
-,
positioning slot Q and positioning an @ i~i~ 21
11
* When the eyepiace positioning pin is insem into the
Ioww dot on the tuba, tha m lines in the eyepieca
coincida with the vibration direction of polarizer and
analyrer at 0' settings. When In- into the other
slot, the mass lines am at 4g to the direction of
vibration. (This is the same with the monwlar
tube.)
Fig. 2
Them iwrt the
eyepiece into the left tube.