Olympus DP73 User manual

DP73
This instruction manual is for the Olympus microscope digital camera DP73. To ensure the safety, obtain
optimum performance and familiarize yourself fully with the use of this camera, we recommend that you
study this manual thoroughly before operating the camera.
For image operations including recording, editing and saving, please refer to the online manual for the
cellSens / OLYMPUS Stream Software.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
INSTRUCTIONS
MICROSCOPE DIGITAL CAMERA
A X 8 2 0 8
Optical Microscope Accessory

In accordance with European Directive on Waste Electrical and Electronic Equipment, this symbol indicates that the
product must not be disposed of as unsorted municipal waste, but should be collected separately.
Refer to your local Olympus distributor in EU for return and/or collection systems available in your country.
For Korea only
B급 기기 (가정용 방송통신기자재)
이 기기는 가정용(B급) 전자파적합기기로서 주로 가정에서 사용하는 것을
목적으로 하며, 모든 지역에서 사용할 수 있습니다.
This product is applied with the requirements of standard IEC/EN61326-1 concerning electromagnetic compatibility.
- Immunity Applied to industrial and basic environment requirements.
NOTE: This product has been tested and found to comply with the limits for a Class A digital device, pursuant to Part 15 of the
FCC Rules. These limits are designed to provide reasonable protection against harmful interference when the product is
operated in a commercial environment. This product generates, uses, and can radiate radio frequency energy and, if not
installed and used in accordance with the instruction manual, may cause harmful interference to radio communications.
Operation of this product in a residential area is likely to cause harmful interference in which case the user will be required
to correct the interference at his own expense.
FCC WARNING: Changes or modifications not expressly approved by the party responsible for compliance could void the user’s
authority to operate the product.

DP73
1 Intended use ...............................................................................................................................................................................................7
2 Conformity of the System ................................................................................................................................................................7
3 Handling Precautions..........................................................................................................................................................................15
4 Maintenance and Storage ..............................................................................................................................................................16
1 SYSTEM CHART ...............................................................................................................................17
2 NOMENCLATURE ............................................................................................................................18
2-1 DP73 / DP73WDR (For Desktop PC) ........................................................................................................................................................18
2-2 [OPTIONAL] DP-PXU PCIe Extension Unit (For Laptop PC) ..................................................................................................20
3 HARDWARE INSTALLATION ....................................................................................................21
3-1 Installing the Camera Head ............................................................................................................................................................................21
3-2-a
Installing the PCIe Interface Board to Desktop PC (DP73 / DP73WDR) ...................................................................... 23
1 Installing the Low-Profile Bracket .............................................................................................................................................23
2 Installing the PCIe Interface Board .........................................................................................................................................24
3 Connecting the Interface Cable ................................................................................................................................................26
IMPORTANT -- Be sure to read this section for safe use of the equipment. -- 1-16

3-2-b Installing the PCIe Interface Board to Laptop PC (DP-PXU).................................................................................................... 27
1 Installing the Low-Profile Bracket .............................................................................................................................................27
2 Installing the PCIe Interface Board .........................................................................................................................................28
3 Installing the PCIe Extension Box ............................................................................................................................................30
4 Connecting the Interface Cable ................................................................................................................................................31
5 Connecting the PCIe x1 cable ....................................................................................................................................................32
6 Connecting the AC Adapter ..........................................................................................................................................................33
3-3 Connecting the External Trigger Cable ................................................................................................................................................34
4 SOFTWARE INSTALLATION ......................................................................................................35
5 IMAGE ACQUISITION PROCEDURE ...............................................................................37
6 EXTERNAL TRIGGERING ..........................................................................................................39
7 SPECIFICATIONS .............................................................................................................................41
8 TROUBLESHOOTING GUIDE ................................................................................................46
PROPER SELECTION OF THE POWER SUPPLY CORD ............................................................ ...................................................53-56

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IMPORTANT
The microscope digital camera DP73 is designed to be connected to a camera adapter mounted on an Olym-
pus UIS2/UIS series of optical microscope (not applicable to the LB series) for use in recording of microscopic
magnified images at high speed and highest resolution while maintaining high picture quality and high color
reproduction. The DP73 incorporates a variety of functions for supporting image recording under optimum
conditions.
When the microscope digital camera DP73 is used with a camera adapter or a microscope from a manufac-
turer other than Olympus, the optical performance may not be manifested fully.
If the equipment is used in a manner not specified by this manual, the safety of the user may be endangered. In addi-
tion, the equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
The following symbols are used to set off text in this instruction manual.
: Indicates a potentially hazardous situation which, if not avoided, may result in minor or
moderate injury or damage to the equipment or other property. It may also be used to
alert against unsafe practices.
} : Indicates commentary (for ease of operation and maintenance).
CAUTION

2
Caution on Image Data Storage
The recorded image data may be lost (destroyed) in the following cases. Note that Olympus will not assume any liabilities for
the loss (destruction) of recorded data. The image data may also be destroyed by an unexpected cause. It is recommended to
back up the data periodically.
· When the user or a third party services repairs the equipment.
· When the PC is shut down, the AC adapter is disconnected or the power cord is unplugged during recording or era-
sure (formatting) of the PC.
· In case when continuing to save the data for the period exceeding the life time of data storage of USB memory, (ap-
prox. 1 to several years).
· When the equipment fails.
Please note if following cases may occur though the camera is working properly, the image restoration or the compensation for
damages are not guaranteed.
· The image is abnormal.
· File properties e.g. file names, file time, etc. are abnormal.
· The image is lost.
} In general, as the data retention lifetime is set in the electronic memory media, e.g. USB memory, HDD, CD-R, DVD-R, etc.,
the saved files may be lost after several years.
} The image data may disappear (be destroyed) unexpectedly. Be sure to backup the data on regular base.
} Make sure that there is no problem when acquiring the subjects before acquisition. Please note that even though the
problem may occur caused by the acquired images, Olympus will not be responsible for these problems.

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SAFETY PRECAUTIONS
Safety Symbols
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Symbol Explanation
Indicates a non-specific general hazard. Follow the description given after this
symbol or in instruction manual.
Warnings
Warning engravings are placed at parts where special precaution is required when handling and using the microscope.
Always heed the warnings.
Warning engraving position
Rear of the camera head
PCIe extension box
PCIe interface board
If the warning engraving becomes dirty or is peeled off, contact Olympus for replacement.

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Never connect or disconnect the interface cable while the standby switch of the PC is set to ON. Otherwise,
malfunction may result.
1. Before connecting or disconnecting the interface cable, make sure that the standby switch of the PC is set to OFF.
When connecting the interface cable, push in the connector all the way and ensure that the connector will not slip
out before setting the standby switch to ON.
Do not move the PC or apply an impact to it while it is powered ON.
2. The cords and interface cables are vulnerable to bends or twists. Do not apply excessive force to them.
3. To prevent the microscope from toppling down, avoid using microscope attachments that may make the total height
of the microscope above 1 meter when they are attached.
4. When installing the PCIe interface board, be sure to hold it by the edge. Never touch the board surface directly, as
this will lead to malfunction.
CAUTION

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The area (IC) of the PCIe interface board becomes very hot during and immediately after use of the camera.
Be careful never to touch them during and after use.
5. For handling of the PC, refer to the separately provided “PC User’s Manual”.
6. Always use the power cord provided by Olympus. If no power cord is provid-
ed, please select the proper power cord by referring to the section “PROPER
SELECTION OF THE POWER CORD” at the end of this instruction manual. If
the proper power cord is not used, the safety and EMC performance of the
device can not be assured.
7. Always connect the power cord correctly and ensure that the grounding
terminal of the device and that of the wall outlet are properly connected. If the
device is not grounded, our intended electric safety and EMC performance of
the device can not be assured.
8. When installing the DP73, route the power cord away from the lamp house of
the microscope. Should the power cord come in contact with a hot part, the
power cord could melt and cause electric shock.
9. After operation or in case of abnormality, be sure to disconnect the power
cord from the connector on the product or from the outlet.
10. Do not permit tools or metal fragments to get into air vents, or other apertures.
Doing so could cause failure of the PCIe extension box or electric shock to
the user.
Hot part

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PC and Software
The PC data may be destroyed by an unexpected event. Be sure to keep a backup of the data.
1. Olympus will not assume any liabilities for any damage incurred due to the use or non-usability of this system,
including compensation for the lost data.
2. The PC used with this system should set up and run the OS designated by Olympus.
For the OS in the PC, the user is requested to create a backup and retain it carefully. (Olympus does not support the
matters related to the OS including its backup.)
For details on the PC and OS, refer to their respective manuals.
3. Olympus guarantees the quality of this product in the factory shipment condition.
Olympus will not assume any liabilities for the operation errors and functional faults incurred due to the alteration of
the environmental setup (including BIOS change), installation of other software or addition of hardware to the PC by
the user.
4. When the HDD free space reduces, the data processing speed may slow extremely or errors may occur frequently. To
prevent this, delete unnecessary data files frequently. For how to delete data files, refer to the manuals for OS.
5. Never attempt to delete or rename the folders and files installed by the provided installer software. Otherwise, the
software may get unable to be started up.
6. Do not open the housing of the PC and touch the power supply or the circuit board’s heat generating section right
after use as it may burn your hand. Wait until the internal temperature drops sufficiently.
7. Sharp edges inside the PC may cut your fingers, so take extra care.
8. Use a PC that complies with the safety standards of your country.
CAUTION

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1 Intended use
This device is intended to be used for the capture of digital images, but not for clinical diagnostic purposes.
USA: CAUTION:
Federal law restricts this device to sale by or on the order of an appropriately licensed healthcare practitioner.
2 Conformity of the System
Restrictions in Use
1. The applicable camera adapter is the U-TV0.5XC-3, U-TV0.63XC, MVX-TV0.63XC, U-TV1XC or the combination of U-
TV1X-2 and U-CMAD3.
The U-TV0.5XC should not be used because it deteriorates the image flatness.
A camera adapter with a magnification below 0.5X cannot be used because part of image will be obscured.
2. When the DP73 is connected to the rear port of the U-DPT(double port tube) or U-MPH(multi port head), the periph-
eral part of the recorded image may be deteriorated due to the optical performance of the U-DPT or U-MPH.
3. When the U-TV0.5/C-2/U-TV0.5/C-3/U-TV0.63XC (C mount camera adapter) is used, using two or more intermediate
attachments* may obscure the peripheral part of the field of view or may make flare noticeable.
* Example of two intermediate attachments with BX microscope:
Reflected light illuminator + intermediate attachment equivalent to the U-CA.

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4. Under fluorescent ring illumination or other AC-driven illumination such as a phase control light intensity adjusting
illumination system, the following phenomena may be observed by illumination light flickering because of high light
intensity and shortened exposure time :
• Flickering of the displayed image.
• Instability in exposure.
• Hatching patterns in pixel shift recording (4800 x 3600 or 2400 x 1800 pixels, 3CCD mode).
However, provided that the brightness can be adjusted using the light intensity control knob or ND filters, the above
phenomena may be attenuated by adjusting the brightness so that the exposure time exceeds 1/50 sec.
For details on the microscope models using AC-driven illumination, contact Olympus.
5. Combinations of this product and non-Olympus microscopes have not been evaluated extensively. Non-Olympus
microscopes and commercially available C-mount lenses can be used provided that they match a CCD with a size
of no less than 1/1.8 inch and the lens projection length from the C-mount body attaching section is no more than
4.5 mm. However, problems due to optical adaptability, such as shading, may be observed.
6. When the specimen has a low contrast (near transparent) or high reflectance (mirror status) and the aperture iris
diaphragm is stopped down near the smallest aperture, spot flare may be noticeable.
7. When the edge of a non-transmitting object is observed under the STM6 (small measuring microscope) transmitted
illumination, flare may be noticeable due to the difference in brightness between the transmitted sections (over-expo-
sure) and non-transmitting sections (under-exposure). To reduce the flare, set a lower exposure using the exposure
correction function or setting the exposure manually.

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8. When a low-power objective (below 4X) is used, the peripheral part of the field of view may be obscured. In this case,
use an ultralow-magnification condenser (U-ULC-2).
9. When the U-CFU (real time confocal unit) is used, it is required to set the exposure to a longer period than 1/30 sec.
using the manual exposure mode and control the brightness by engaging or disengaging ND filters.
10. When the specimen has large difference in brightness, particularly when the bright part of the specimen comes on
the upper part of the image, red and horizontal flare may be observed. It is noticeable when the AS is being closed.
The flare will get unnoticeable when the AS is being opened, though the flare may not be completely removed.
11. When a specimen with high reflectivity is observed with reflected light brightfield observation through the beam
splitter of the eyepiece/camera light path of a trinocular tube in combination with the U-TV0.5XC camera adapter, the
image in the area outside the CCD’s effective image pickup area may be observed as vague ghosts in the peripheral
area of the visual field of eyepiece.
12. Flare may be produced during reflected light darkfield observation under overexposure To reduce the flare, use the
exposure correction function or reduce the exposure with manual exposure control.
13. During image acquisition with pixel shifting (4800 x 3600 or 2400 x 1800 pixels, 3CCD mode), the image may be
disturbed if the specimen is moved.
14. If the camera or microscope is vibrated during image acquisition with pixel shifting (4800 x 3600 or 2400 x 1800
pixels, 3CCD mode), the image will be disturbed. There are several causes for the vibration which are, the operation
of keyboard/mouse on the same table where microscope equipped with the camera is placed, vibration from the
instrument erquipped with air cooling fan, etc.

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Operating Environment
Temperature: 10 to 35°C.
Humidity: 30% to 80% (without condensation).
See page 41 for details.
Recommended Monitor Specifications
· A monitor with the 1280 x 1024 or larger full-color display capability.
· An Adobe RGB compatible monitor, provided that the camera head is used in the Adobe RGB mode.
Recommended PC Specifications – Desktop PC –
1. PC requirements
PC/AT compatible
CPU Intel® Core i5, Intel® Core i7, Intel® Xeon, (or equivalent)
RAM 4 GB or more
(8 GB recommended for high speed image acquisition)
HDD Free space 1 GB or more
(at the time of installation)
Graphic 1280 x 1024 (min. 1024 x 768) monitor resolution with 32 bit video card with separate
graphics memory (no integrated graphics processor with shared memory)
Extension slot PCI Express Rev. 1.0a or later.
Half-size or low-profile PCIe board compatible (106.7 mm x 174.6 mm)

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OS Microsoft® Windows® 8.1 Professional (64-bit)
Microsoft® Windows® 8 Professional (64-bit)
Microsoft® Windows® 7 Ultimate / Professional SP1 (64-bit)
Microsoft® Windows® 7 Ultimate / Professional (64-bit)
Language: English or Japanese
Power 250 W or more
* There needs to be the FDD power cable, HDD(4-pin) power cable or SATA power cable
which is not occupied.
* Please use computers that conform to the regulations for your region.
2. CPU
We do not guarantee performance if the PC uses a CPU other than or incompatible with Core series or uses a non-
Intel chipset.
3. HDD free space
The HDD free space (the value 1GB or more in Page 9) is the space that does not cause a special problem when
the system is installed or run. The space required for saving an image file in the HDD is slightly more than 5.6 MB
with a 1600 x 1200-pixel (24-bit) non-compressed image and slightly more than 50 MB with a 4800 x 3600-pixel
(24-bit) non-compressed image. In consequence, the HDD should also provide a considerably large space for saving
these image files.
When saving movies in the HDD stack, the space required for saving a movie is about 80 MB (max.) per second. The
movie recording time is limited according to the HDD free space.

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4. RAM
If a RAM other than a PC2700 or greater, dual-channel RAM is used, the full-size live frame rate may drop.
5. Monitor
Us an Adobe RGB compatible monitor when using the camera head in the Adobe RGB mode.
Optimum color reproduction is not available if the sRGB/Adobe RGB setting of the camera head does not match the
setting of the monitor.
6. Sequential connection of PCIe units
Up to two PCIe units including the DP73 and a PCI interface board of the DP72/DP71/DP70/DP30BW or FV1000
(FV10-ASW-V1.5 or later) can be connected in series. Series connection of the FV300/FV500 is not possible.
However, their simultaneous operation is not available so it is required to select either PCI interface operation.
7. Power supply
The PCIe interface board should be powered by connecting the FDD power supply connector from the ATX power
supply in the PC.
If your PC does not have an available FDD power cable or the FDD power cable is too short, use the provided
HDD(4-pin) to FDD power conversion cable for the power supply.
And if your PC does not have an available HDD power cable (4-pin), use the provided SATA-to-HDD(4-pin) power
conversion adapter and HDD(4-pin)-to-FDD power conversion cable for the power supply.

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Recommended PC Specifications – Laptop PC –
1. PC requirements
PC/AT compatible
CPU Intel® Core i5, Intel® Core i7, Intel® Xeon, (or equivalent)
RAM 4 GB or more
(8 GB recommended for high speed image acquisition)
HDD Free space 1GB or more
(at the time of installation)
Graphic On-board graphic with 1280 x 1024 or more and 32-bit color capability.
Card slot ExpressCard/34 or ExpressCard/54
OS Microsoft® Windows® 8.1 Professional (64-bit)
Microsoft® Windows® 8 Professional (64-bit)
Microsoft® Windows® 7 Ultimate / Professional SP1 (64-bit)
Microsoft® Windows® 7 Ultimate / Professional (64-bit)
Language: English or Japanese
* Please use computers that conform to the regulations for your region.
2. CPU
We do not guarantee performance if the PC uses a CPU other than or incompatible with Core series or uses a non-
Intel chipset.

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3. HDD free space
The HDD free space (the value 1GB or more in Page 9) is the space that does not cause a special problem when
the system is installed or run. The space required for saving an image file in the HDD is slightly more than 5.6 MB
with a 1600 x 1200-pixel (24-bit) non-compressed image and slightly more than 50 MB with a 4800 x 3600-pixel
(24-bit) non-compressed image. In consequence, the HDD should also provide a considerably large space for saving
these image files.
When saving movies in the HDD stack, the space required for saving a movie is about 80 MB (max.) per second. The
movie recording time is limited according to the HDD free space.
4. RAM
If a RAM other than a PC2700 or greater, dual-channel RAM is used, the full-size live frame rate may drop.
5. Monitor
Us an Adobe RGB compatible monitor when using the camera head in the Adobe RGB mode.
Optimum color reproduction is not available if the sRGB/Adobe RGB setting of the camera head does not match the
setting of the monitor.
6. ExpressCard
The ExpressCard tends to slip out easily from the PC. Be sure not to disconnect the ExpressCard during operation.
For protection from possible damage, do not apply excessive force to the ExpressCard. Insert or remove the Express-
Card after the PC is shut down.

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3 Handling Precautions
1. This camera head is a precision instrument. Handle it with care and avoid subjecting it to a sudden or severe impact.
2. The image displayed on the monitor may be affected when it is used at a place close to equipment generating
strong electromagnetic waves. This is not a malfunction and will not affect the actual image being recorded. To avoid
interference during operation, keep the system far from any source of electromagnetic waves.
3. As the camera head is heavy, hold it carefully not to drop when mounting or storing.
4. Do not use the camera in areas where it may be subjected to direct sunlight, high temperature, humidity, dust, or
vibrations. (For the operating environment conditions, see chapter 7, “SPECIFICATIONS“ on page 41.)
5. Install the PCIe extension box on a sturdy, level table or bench.
6. Be sure to install the PCIe extension box without blocking the opening for ventilation provided on it.
7. The camera head needs to be calibrated periodically (approx. every 3 months) for the level variations caused by
the influence of cosmic rays. For the calibration method, refer to the CCD Calibration in the online manual for the
cellSens or OLYMPUS Stream software.
8. As a countermeasure against PC virus infection, it is recommended to install an anti-virus software on the PC. Opera-
tion speeds of the cellSens/OLYMPUS Stream may slow down depending on the anti-virus software.
9. For operating the PC, refer to the instruction manual of the PC in use. Pay attention to the precautions and warnings,
etc. in the manual.
10. If cables are connected incorrectly, the product may malfunction. Be sure that every unit is connected correctly before
operating the product.

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4 Maintenance and Storage
1. Do not leave stains or fingerprints on the lenses and filters. Blow away dust with a commercially available blower and
gently wipe the lens or filter with a piece of cleaning paper (or clean gauze).
For wiping fingerprints and oil stains, use a piece of cleaning paper moistened with commercially available absolute
alcohol.
Since the absolute alcohol is highly flammable, it must be handled carefully. Be sure to keep it away from open
flames or potential sources of electrical sparks --- for example, electrical equipment that is being switched on
or off, which could cause ignition of a fire. Also remember to always use absolute alcohol only in a well-ven-
tilated room.
2. Do not use organic solvents, which cause painted and plastic parts to deteriorate. Do not use organic solvents to
clean the product components other than the glass components. To clean them, use a lint-free, soft cloth slightly
moistened with a diluted neutral detergent.
3. Do not disassemble any part of the camera as this could result in malfunction or reduced performance.
4. When the camera is removed from microscope for storage, be sure to put the C-mount cap included in the camera,
which is necessary to protect the IR cut filter provided inside.
5. When the camera is stored, be sure to keep the C-mount portion downward, since it tends to roll over.
6. Before disposing of this product, be sure to follow the regulations and rules of your local government.
CAUTION