Olympus fsx100 User manual

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2
Introduction
SAFETY PRECAUTIONS
Observation Methods
Introduction
Fluorescence
observation
This method utilizes the
fluorescence generated
when the specimen is
irradiated with strong light
to observe the specimen
structure or identify the
substance according to the
presence/absence and color
tone of the fl uorescence.
Phase contrast
observation
T h i s m e t h o d m a k e s i t
possible to observe non-
stained specimen (living
specimen) or nearly
transparent specimen by
enhancing the contrast
between brightness and
darkness.
Bright Field
observation
This is the most general
method for use in obser-
vation of stained specimens
and contrasted specimens.
Observation/Acquisition Flow Main Controls
During observation and acquisition, the cover is
kept closed to avoid the risk on the user. However,
when placing or replacement, the cover should be
opened so care is required in these operations.
Caution against
high temperature
This section accommodates
the light source bulb and may
become hot.
Caution against
fi nger being caught
Do not approach your hand or
fi nger to the motorized stage
and motorized revolving
nosepiece.
Caution against
direct view
This section outputs bright
light during fl uorescence
observation. Be careful not to
stare it directly.

3
Main Controls
Introduction Observation/Acquisition Flow
Main Controls
Controrls
Main switch
： ON
： OFF
Close lever
Push down to close the
cover.
Open button
Push down to open the
cover.
STATUS LED
Lights in green: Sub-switch ON
Blinks in green: Sub-switch OFF.
Sub switch
To turn OFF, keep it depressed for
5 seconds.
FL BURNER LED
Lights blue when the fl uorescence
observation light is ON.
Cover
The controls other than the
above can be adjusted and
set from the software.
(The operations are easy
thanks to the operation guide
display.)
Do not set the main switch to OFF for at
least 3 minutes after setting
the sub-switch to OFF to allow the bulb to
cool down.

4
Observation/Acquisition Flow
IntroductionMain Controls
Observation/Acquisition Flow
Starting the
System
p. 6
Placing the
Specimen
p. 7
Selecting the
Observation/
Acquisition Methods
p. 8
This section describes the basic
operation fl ow in sequence together
with the monitor screen displays.

5
Observation/Acquisition Flow
Main ControlsIntroduction
When using the unit for the fi rst time:
Activation is required.
(For the Activation procedure, refer to
the Instruction Manual [Hardware].
The description in this section assumes
that the following preparation work has
been completed.
・ The cables are connected properly.
・ The software has been installed.
・ The lamp is attached.
(For the operating procedures for the
above, refer to the Instruction Manual
[Hardware].
CAUTION
Observation/
Acquisition
Procedure
p. 9
Operation Guide
Display
p. 10
Expanding
the Scope of
Acquisition
p. 11

6
1
5
6
2
Observation/Acquisition Flow
Main Controls Introduction
Double-click on the FSX-BSW icon on
the desktop to launch the software.
1
5
6
2
3
4
1. Starting the System
Observation/Acquisition Flow
Set the main switch to “ l ” (ON).
Push the sub switch to turn it on.
Set the main switches of the computer
and monitor to ON.
Log on Windows Vista with your own
user ID.
The splash screen appears on the
monitor screen.
The image data acquired with this unit
and the acquisition settings are saved
on a per-user ID basis.
Now the system has started up.

7
2. Placing the Specimen
The specimen can be placed in position
when the instruction to place the
specimen is displayed.
Push down the
open button 2,
open the cover and
place the specimen.
1
4
5
5
4
3
3
Push down the close lever and close
the cover.
Click on the button
and open the Acquisition menu.
Push in the lock lever toward the left
and hold it in the pushed-in position.
Pull out the moving lever toward you
(all the way until it stops).
Pull out the slide glass holder toward
the right to set the slide glass in
position.
Push down the close lever and close
the cover.
Click on the button
and open the Acquisition menu.
Push in the lock lever toward the left
and hold it in the pushed-in position.
Push in the moving lever toward the
deep (all the way until it stops).
Observation/Acquisition Flow
Main ControlsIntroduction
1
3
3
5
6
6
4
4
7
7
2
Place the slide glass.
Be sure to place it with the cover glass
facing down.
Slide glass specimen
Now the specimen has been placed in position.
Place the dish by applying against the
dish stopper 5.
Φ 35 mm dish specimen

8
Observation/Acquisition Flow
Main Controls Introduction
3. Selecting the Observation/Acquisition Methods
Observation/Acquisition Flow
Click on the buttons enclosed in yellow frames.
Select the
observation method.
Fluorescence & Phase
contrast observation /
Phase contrast
observation /
Bright Field observation
Select the acquisition
method.
Single /
Time-Lapse /
Z-Stack /
Stitching
(Scheduled to be available
in March 2009)
Select the fl uorescence color to be
used as the observation standard
or the phase contrast.
Green / Red / Blue / Phase contrast
Start button The selection
summary is
displayed.
The subsequent description in this manual assumes that the following
selections are made.
Observation method: Fluorescence & Phase contrast observation
Observation standard color: Green
Acquisition method: Single
Now the observation and acquisition methods have been selected.

9
4. Observation/Acquisition Procedure
Adjust the focus and magnifi cation.
Optimize the image.
Fluorescence only Overlap the
fl uorescence color or phase contrast
images and confi rm the acquisition
condition.
Click on to acquire
the image.
Observation/Acquisition Flow
Main ControlsIntroduction
5
6
8
7
Locate the observation target fi rst.
Move the observation position
(with drag & drop) to locate the
observation target.
Click on to go
to the next screen.
2
1
See next page for the operation guide display.
Tightly target at the observation
area in the macro image.
:Move the target frame.
：Enlarge or reduce
the target frame.
Click on to go
to the next screen.
4
3
Now the acquisition is complete.
Adjust the image and acquire it.

10
Observation/Acquisition Flow
Main Controls Introduction
5. Operation Guide Display
Observation/Acquisition Flow
The operation
guide shows the
basic procedure
for completing
acquisition with
the selected
observation/
acquisition methods.
Click to view the description
text.
Click to view the one-point
tip.
Click to go to the next screen.
Description text.
The Online Help gives more detailed explanations.

11
Observation/Acquisition Flow
Main ControlsIntroduction
Instruction Manual
[Hardware]
Operation Guide/
Online Help
This unit incorporates more other functions.
For details, please refer to the separate Instruction
Manual [Hardware] as well as the Operation Guide/
Online Help for the FSX-BSW Software.
6. Trying to Expand the Scope of Aquisition
Fluorescence multi acquisition
Bright Field
acquisition
Z-Stack
acquisition
Time-Lapse
acquisition
Phase Contrast
acquisition
Features for trying to expand
the range of acquisition:

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