Olympus BHS User manual

This instruction manual has been written for the use of the Olympus System Microscope Model
BHS. It is recommended that you read the manual carefully in order to familiarize yourself fully
with the use of the microscope, so that you can obtain optimum performance from it.
IMPORTANT
Observe the following points:
m Operation
Always handle the microscope with the care it deserves, and avoid abrupt motions.
Avoid
the use of the microscope in direct sunlight, high temperature and humidity, dust
and vibration.
Only use the tension adjustment ring for altering the tension of the coarse adjustment
knobs. (Do not twist the two coarse adjustment knobs in opposite directions simultaneous-
ly, as this will cause damage.)
Make sure that the voltage selector switch on the base plate is set to conform with the local
mains voltage.
Make it a point of grounding the microscope to prevent electric accidents.
m Maintenance
1. Lenses must always be kept clean. Carefully wipe off oil or fingerprints deposited on the
lens surfaces with gauze moistened with a small amount of xylene, alcohol or ether.
Do not use organic solutions to wipe the surfaces of various components. Plastic parts,
especially, should be cleaned with neutral detergent.
Never disassemble the microscope for repair. Only authorized Olympus service personnel
should make repairs.
The microscope should be covered with the vinyl dust cover provided and stored in a place
free from humidity.

VI.
VII.
STANDARD EQUIPMENT
NOMENCLATURE
ASSEMBLY
IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS
OPERATION
CONTENTS
ο σ
F
WN
A. Switching on the Light Source
E
G
OPTICAL DATA
TROUBLESHOOTING . .
Voltage Adjustment and Light Intensity
Placement of a Specimen Slide
二 10
Specimen Slide
Observation Tube
1. Interpupillary Distance Adjustment
2. Diopter Adjustment
3. Light Path Selector
Condenser Adjustment
1. Condenser Centration
Focusing Adjustment
1. Tension of Coarse Adjustment Knobs and Fine Adjustment
Use of Rubber Cap for Fine Adjustment Knob
2. Pre-Focusing Lever
3. Adjustment of Stage Block Height
Use of Immersion Objectives
Photomicrography

I. STANDARD EQUIPMENT
Model
Component
BHS-112 |BHS-113 |BHS-312 | BHS-313
Microscope stand BHS-F 1 1 1 1
Binocular
ES BH2-B130 1 1 0 0
Observation tubes
Trinocular
tube BH2-TR30 0 0 1 1
Revolving Ouintuple BH2-5RE 1 0 1 0
Sextuple BH2-6RE 0 1 0 1
Square mechanical stage with
right-hand low drive coaxial BH2-SVR 1 1 1 1
controls
Swing-out
cöndenser BH2-SC 1 1 1 1
Condensers
Achromatic/
Aplanatic BH2-AAC 0 1 0 1
condenser
Halogen lamp housing BHS-LSH 1 1 1 1
Halogen bulbs 12V100WHAL-L 2 2 2 2
DPlan 4X,
D Plan 10X,
D Plan 20X, Teach 0 Teach 0
D Plan 40X,
D Plan 100X (oil)
Objectives
S Plan 4X,
S Plan 10X,
S Plan 20X, 0 Teach 0 Teach
S Plan 40X,
S Plan 100X (oil)
Eyepieces WHK10X 2 2 2 2
Photo eyepiece NFK3.3X 0 0 1 1
Line cord UYCP 1 1 1 1
Filter KB-4 1 1 1 1
Immersion oil, bottled 1 1 1 1
Vinyl dust cover 1 1 1 1
Allen wrench 1 1 1 1

|. NOMENCLATURE
The Model BHS consists of various components and interchangeable accessories as shown in the
photo below. A wide variety of combinations, standard or optional, is available according to your
requirements.
Eyepiece
Observation tube
Microscope stand
For biological use.
Revolving nosepiece
Halogen lamp
Objective housing
Condenser
Base
Em

LA ASSEMBLY
This picture illustrates the sequential procedure of assembly. The numbers indicate the
order of assembly of various components. Remove dust caps before mounting components.
Take care to keep all glass surfaces clean, and avoid scratching the glass surfaces.
NOTE: For numbers @, @, @ and @ please refer to explanations in detail on the next
page.
Eyepiece
Nosepiece
y
4
Observation tube
B Objective
á ==7 Circular dovetail mount
Specimen
holder o 3 .
en © Observation tube clamping screw
em Nosepiece clamping screw (on the opposite side)
© mtos.
Condenser clamping screw
Stage
mounting
Halogen
bulb
Halogen
lamp
dovetail
一
一
生
@
0
housing
AZ
2
Li
Condenser — m
>
Collector
lens
Clamping
(The
N.A.
scale
ρα
engraved on the con- Microscope stand a
denser
should
face
the
To
AC
microscope
front.)
es
Line cord © outlet

= Explanations in detail
@ Mounting the halogen bulb
1) Releasing the bulb clamping levers @ of
the collector lens @ in the direction of
the arrow, insert two contact pins of the
halogen bulb into the socket ©. (Fig. 1)
(Recommended to use a glove or gauze to
handle the halogen bulb.)
2) Secure the bulb in position with the two
levers.
* Before use, wipe off any fingerprints or
stains on the bulb.
© Mounting the stage
1) Loosen the stage clamping screw © by
rotating counterclockwise. (Fig. 2)
2) Insert the stage into the mounting dove-
tail of the microscope stand slowly and
lock with clamping screw.
@ Mounting the revolving nosepiece
1) Loosen the nosepiece clamping screw ©.
(Fig. 3)
2) Aligning the nosepiece dovetail slide to
the mounting block ©, push in the nose-
piece slowly all the way.
© Mounting the observation tube
1) Loosen the clamping knob © fully. Pull
spring-loaded clamping knob ©. This
will cause the locating pin @ to withdraw.
(Fig. 4) If the pin does not, loosen the
screw further until the pin withdraws.
2) With clamping knob © pulled out, insert
the circular dovetail of the observation
tube into the ring dovetail.
3) Tighten the clamping knob.

IV. IDENTIFICATION AND FUNCTION OF VARIOUS COMPONENTS
Light path selector knob
The knob can be operated
in 3 positions to deflect the
light as desired.
Tension adjustment ring
Clockwise rotation increases
coarse adjustment tension.
Coarse
Specimen holder y adjustment knob
Coarse adjustment
range: 26mm
Stage clamping screw
Loosen
the
screw
and
.
the
stage
can
be
rotated.
Fine
adjustment
knob
- Graduated in increments
Condenser
centering
of
2u.
knobs
Y-axis low drive
control knob
Y excursion range:
Se. Filter mount
Accepts the ND
filter holder
X-axis low drive
control knob
X excursion range:
76mm.
Main switch
ND filter holder*
Filter diameter 45mm.
Voltmeter
Sliding voltage control lever
Voltage lowers as the lever is
pulled toward the microscope
front.
*Do not disengage the ND filter for about a few minutes after the use, in order to give time to
cool. Other filters (e.g. KB-4 provided, etc.) should be inserted into the filter mount at the light
exit on the microscope base.

Photo tube
Diopter adjustment
ring
Condenser height | = |
adjustment knob
Pre-focusing lever
Aperture iris
diaphragm ring
Circuit breaker*
Filter mount
Field iris diaphragm ring
Arrow mark @ — O indicates
increase in diaphragm diameter.
Line cord ==
*This breaker protrudes its central part to cut off the electrical power in case of the dimmer
circuit trouble (due to short circuit, etc.) or over current. To restore the breaker, press the
central part. If the breaker is actuated again, disconnect the line cord from the AC outlet and
contact the Olympus Service Center.

Summary of Putting the Microscope into Operation
Match the voltage selector switch to local mains voltage (page 9).
Switch on the light source (page 9).
Place a specimen slide on the mechanical stage (page 9).
Coarse focus with the 10X objective.
Make interpupillary distance and diopter adjustments (page 11).
Adjust the condenser position (page 12).
Swing in the desired objective.
THT
MOL
BP
Adjust light intensity.
Fine focus.
y. Adjust aperture iris diaphragm and field iris diaphragm (page 12).
Model BHS
Adjustment of Illumination System for Various Objective Powers
}
| Condenser
Objective ic.
Lu FORTS Abbe Swing-out Low power
magnification čondensér condenser condenser condenser
BH2-AAC BH2-CD BH2-SC BH2-UL-C
1X
2x
Swing
out
top
Compatible
ax
4x
lens
10X
28% Compatible m
40x
Compatible
Swing
in
top
lens
6&
||
|...
100X
*
*N.A. is somewhat low, but still compatible with a 100X objective.
**To obtain better illumination for photomicrography with a 2X objective, it is recom-
mended to use the ultra low condenser BH2-ULC, optionally available.
(Cut off this page at dotted line and put it on the wall near the microscope for use as a reminder of micro-
scopic procedure.)
OLYMPUS

V. OPERATION
A. Switching on the Light Source
1) Ascertain that the voltage selector switch ®
is set to conform with the local mains volt-
age. (Fig. 5)
If the switch is not correctly set, adjust it by
means of the Allen wrench provided or a
screwdriver.
2 Place the sliding voltage control lever on the
right side of the microscope base to a posi-
tion closest to you (low voltage position).
Switch on the light source. (Fig. 5)
[ Voltage Adjustment and Light Intensity |
As you push the control lever@ in the direc-
tion of the arrow in order to obtain increas-
ing intensity (Fig. 6), the LED readout @
will display the lamp voltage.
* Two LEDs on the left side indicate the
voltage from OV to 6V, and twelve LEDs on
the right side from 6.5V to 12V in 0.5V
increments. The indication with the letters
“PHOTO” can be used as a guide line for
color photomicrography.
B. Placement of a Specimen Slide
1
Rotate the coarse adjustment knobs ® in
the direction of the arrow to rack down the
stage so that a specimen slide can be placed
on the stage. (Fig. 7)
NOTE: The rotation of the coarse and fine
adjustment knobs in the direction of
the arrow will rack down the stage.
2) Opening the spring-loaded finger of the
specimen holder with one hand, place a
specimen slide inside the holder. (Fig. 8)
When the slide comes in contact with the
back of the specimen holder, slowly return
the spring-loaded finger.
WARNING: If the spring-loaded finger is
returned quickly, it may cause
damage to the specimen slide.
Fig. 7
Fig. 8

© An Olympus objective engraved ‘160/0.17" requires a cover glass of 0.17mm thickness.
If the numerical aperture of the objective is 0.7 or higher (except immersion objectives) and
no correction collar is provided, the resolving power deteriorates to a great extent if cover
glass thickness deviates from the above listed value.
NOTE: In some countries a 0.17mm cover glass corresponds to a designation of #1%.
@ A cover glass (0.4mm thick) for blood counting, etc. can be used with Olympus objectives
of the working distance longer than 0.4mm. (See VI. OPTICAL DATA at page 16.)
[Specimen Slide
®@ Specimen slides 0.8mm to 1.2mm thick are
recommended for Olympus objectives.
3) Bring the portion of the specimen for obser-
vation into the light path by means of the
low drive control knobs. (Fig. 9)
* Tighten the stage clamping screw © in the
microscope front.
[Stage
© The specimen holder can accommodate two
standard specimen slides simultaneously.
@ The specimen holder is removable to obtain
a large unobstructed stage surface to hold
specimens up to 55mm x 85mm.
@ To rotate the stage loosen the stage clamping
screw © and holding this screw, rotate the
stage into the desired direciton. (Fig. 9)
O Stage clips @®, for use with immersion objec-
tives. (Fig. 10)
A pair of stage clips are optionally available
to hold the specimen on the stage, eliminat-
ing a specimen drag caused by immersion oil
between slide and stage surface. The clips
can be inserted into the holes provided on
the specimen holder.
Fig. 10

C. Observation Tube
. Interpupillary Distance Adjustment
1) Click the 10X objective into position.
2) Looking through the eyepieces with both
eyes, adjust the interpupillary distance of
the binocular tube by adjusting the
knurled dovetail slides D of the right and
left eyepiece tubes with both hands until
perfect binocular vision is obtained. (Fig.
11)
2. Diopter Adjustment
1) Look at the image through the right eye-
piece with your right eye and focus on
the specimen with the fine adjustment
knobs.
2) Next, look at the image through the left
eyepiece with your left eye and rotate the
diopter adjustment ring © to focus on
the specimen without using the coarse
and fine adjustment knobs. (Fig. 12)
Fig. 12
3. Light Path Selection
1) The trinocular tube is provided with a
light path selector knob © to direct the
light to the observation tube and/or to
the photo tube in 3 positions (Fig. 13)
Indicator Plate
Knob
Position
|
Pushed in all the way Pulled out halfway Pulled out all the way
(v) (С. М)
Amount i 20% into binocular tube ο,
of light 100% into binocular tube 80% into photo tube 100% into photo tube
© Observation | © Observation of exces- | Photomicrography of
_ | _ sively bright specimens | dark specimens
| Applica | © Dark specimens | © Photomicrography (fo-
cusing through the bi- |
| nocular tube)
The indicator plate is provided at the knob port to summarize the usage of the above
table; it can be consulted before operating the knob.
V: Viewer (white letter)
C.V: Camera & viewer (yellow-green letters)
C: Camera (red letter)
The colors of the letters correspond with the color bands on the knob shaft.
11

D. Condenser Adjustment
1, Condenser Centration
1) Stop down the field iris diaphragm with
knurled ring ® by rotating in the direc-
tion of the arrow. (Fig. 14)
2) Use the condenser height adjustment
knob @ to move the condenser up and
down until an image of the field dia-
phragm can be seen clearly in the eye-
pieces. The rotation of the knob in the
direction of the arrow lowers the con-
denser.
Field iris diaphragm image
Field of view
Fig. 15
3)
Bring
the
field
iris
diaphragm
image
into
the
center
of
the
field
of
view
with
the
two
con-
denser
centering
knobs
@).
(Fig.
14)
4)
Widen
the
diameter
of
the
iris
diaphragm
progressively.
If
the
polygonal
image
of
the
iris
diaphragm
becomes
inscribed
in
the
field
it
means
that
the
field
diaphragm
is
centered.
(Fig. 15)
%*
If
the
specimen
slide
used
is
thicker
than
1.2mm,
you
cannot
obtain
the
image
of
the
field
iri
phragm.
Especially
for
photomicrography,
use
a
specimen
slide
0.9mm
to
1.2mm
as
much
as
possible.
Field Iris Diaphragm
@ The field iris diaphragm controls the diameter of the ray bundle impinging on the speci-
men surface and therefore, by stopping down the field diaphragm until it is slightly
larger than the field of view, it can reduce stray light, which in turn increases image defi-
nition and contrast.
Aperture Iris Diaphragm
@ In order to achieve optimum objective performance, the opening of the aperture iris
diaphragm should be matched to the numerical aperture of the objective in use. It is
often preferable, however, to stop down the aperture diaphragm slightly more than in-
dicated by the objective N.A. This will result in better image contrast, increased depth of
focus and a flatter field.
@ After completing focus adjustment, re- < B 70 — 80%
move one of the eyepieces from the obser- ; p 7207508
vation tube and look into the empty
eyepiece tube. As you stop down the aper- DPening of the
ture iris diaphragm, the image of the iriş aperture diaphragm 人
diaphragm can be seen in the objective
pupil, Adjust the opening of the dia-
phragm to match the N.A. of the objective
in use. If the specimen is low in contrast,
it is recommended to stop down to 70%
~80% of the objective N.A. (Fig. 16)
Objective exit pupil
Fig. 16
12

E. Focus Adjustment
1. Tension of Coarse Adjustment Knobs and
Fine Adjustment.
Although the tension of the coarse adjust-
ment knobs has been already adjusted for
optimum performance by the manufacturer,
it is possible to personally adjust the tension
of the coarse adjustment for either heavy or
light movement depending on the operator's
preference by rotating the tension adjust- =
ment ring ©. (Fig. 17) Fig. 17
The ring can be rotated by inserting a screwdriver into one of the holes on the periphery of
the ring. The clockwise rotation (in the direction of the arrow) tightens the coarse adjust-
ment knobs. Do not loosen the ring too much, because the stage may drop or the fine
adjustment knobs may slip.
NOTE: Do not rotate the right and left coarse adjustment knobs in the opposite directions
simultaneously. М the stage drops and the specimen goes out of focus, the tension
adjustment ring is too loose. Tighten the ring.
Rubber Cap for Fine Adjustment Knob
Attaching this cap over the fine adjustment
knob increases the sensitivity of the fine focus-
— ing motion. (The rubber cap is optionally
available.)
2. Pre-Focusing Lever
This lever @ is provided to prevent possible
contact between specimen and objective as
well as to simplify coarse focusing. (Fig. 18)
The lever is locked after coarse focus has
been accomplished. This prevents further
upward travel of the stage by means of the
coarse adjustment knobs, and automatically
provides a limiting stop if the stage is lowered
and then raised again. The pre-focusing lever
does not restrict fine focusing.
3. Adjustment of Stage Block Height
In addition to the vertical movement of the
stage by means of coarse and fine adjust-
ments, the stage block height can be changed
for observation of specimens which are
thicker than standard slides, e.g. chambers,
flasks, etc. with much larger thickness.
The stage block height can be adjusted by
loosening the stage block locking screw with
the Allen wrench provided and retightening
it at the appropriate position. (Fig. 19)

F. Use of Immersion Objectives
1) Focus the specimen with a low power objective.
2) Put a drop of immersion oil on the specimen slide and the front lens of the immersion
objective.
3) Turn the revolving nosepiece to bring the immersion objective into the light path, and focus
with the fine adjustment knobs.
NOTE: © For immersion condensers such as an achromatic-aplanatic condenser or Abbe
condenser, remove the specimen from the mechanical stage and place a drop of
immersion oil on the front lens of the condenser. Then, place the specimen on
the stage and slowly raise the condenser until firm contact with the underside of
the specimen slide is made.
© Care should be taken to prevent oil bubbles from forming in the oil film between
condenser and specimen slide. If any, re-apply immersion oil, for these bubbles
greatly deteriorate the lens performance.
© After use carefully wipe off the immersion oil deposited on the lens surfaces
with gauze moistened with xylene. Never leave oil on the lens surfaces after use
as oil remnants will seriously impair the performance of the lens system.
G. Photomicrography
The Olympus Photomicrographic Equipment Model PM-10AD is uniquely qualified to be
used with the BHS microscope for routine and advanced photomicrography. A separate,
detailed instruction manual is available for the PM-10AD camera system.
For quick reference, however, you may want to refer to the following pointers when using
the PM-10AD.
1, Photographic Eyepiece
Use NFK photo eyepieces for photomicro- ©
graphy.
Insert the eyepiece @ into the eyepiece tube |
of the photo tube. (Fig. 20)
2. Mounting the Photographic Unit
Slip the body of the photographic unit over the
photo tube. Align the dots on photo tube and
the PM-10AD body and clamp the camera unit
to the photo tube. (Fig. 21)
3. Setting the Light Path Selector
Refer to section C.3. on page 11. Fig. 21

4. Color and B & W Photomicrography
a) Color photomicrography
* Voltage setting ........ Within a range as marked with the letters “PHOTO” on
the voltmeter.
© EUR ran vu sara é Insert the LBD-2 filter into the filter mount at the light
exit on the microscope base. (in case of daylight film)
+ Lightintensity ........ Should be adjusted by means of the ND filters rather
than by means of voltage change.
The range of voltage setting mentioned above indicates a guide line for color photomi-
crography. If the photograph, taken this way, however, gives bluish tint, lower the
voltage slightly, and if it gives reddish tint, raise the voltage slightly.
b) Use of contrast filters for B & W photomicrography
© Green filter ..... This filter is most recommended, since it aids in obtaining photo-
micrographs of highest resolution and maximum contrast.
Recommended as contrast filter when it is desirable to emphasize
the blue color in the stained portion of a specimen.
© Cobalt filter .... Not generally recommended for photomicrographic purposes
since it reduces both resolution and contrast. Use it for specific
applications only when it is desirable to emphasize yellow in the
stained portion of a specimen.
© Nofilter ....... A photograph is taken with less contrast than with the green filter.
© Yellow filter
5. Focusing Procedure
Use the field of view eyepieces for focusing on the film plane. Each field of view eyepiece
has a focusing front lens and a reticle with 4 frames, each frame indicating the area covered
by a specific power NFK photo eyepeice. (Fig. 22).
The number at each frame indicates the
magnification of the photo eyepiece. The
image in the field of view eyepiece and the
image on the film plane are in focus at the
same time. Several type field of view eye-
pieces are available, according to the film
size employed.
Fig. 22
Field of view eyepiece | 35WHK10X | PWHK10X 4X5WHK10X | MHWHK10X
35mm Back | 3% x 4%" 4"x 6" 16mm Bolex
Polaroid Sheet Film or camera
Acme camera Back Polaroid Film | 120 Roll Film
Holder Holder
1) Select the field of view eyepiece matching the camera back in use and insert it into the
right eyepiece tube of the trinocular tube, aligning locating groove and locating pin.
2) While looking through the field of view eyepiece, rotate the eyepiece front lens in screw
mount to focus on the double cross lines in the field. For sharp focusing with objectives
4X or lower, the focusing magnifier FT is recommended
3) Bring the specimen detail to be photographed within the frame corresponding to the
power of the NFK eyepiece in use and focus on the specimen with the microscope fine
adjustment knobs. Make sure the light path selector knob on the observation tube is
either on the white (V) or yellow-green (CV) band.
4) It is recommended to tighten the tension adjustment ring considerably to prevent the
stage from dropping during long exposures.
15

VI. OPTICAL DATA
bjective Type D Plan Ach. S Plan Ach.
Magnifi- + ㆍ
catión: 4x | 10X | 20X | 40X |100X*| 4x | 10X | 20X | 40X |100X
Ν.Α. 0.10 | 0.25 | 0.40 | 0.65 | 1.25 | 0.13| 0.30 | 0.46 | 0.70 | 1.25
W.D.(mm)| 7.03 | 7.4 0.83 | 0.23 | 0.17 | 15.5 7.5 1.5 0.5 0.17
Focal length
(mm)
Resolving
Eyepiece \| power (11) 3.36 1.34 | 0.84 | 0.52 | 0.27 2.58 1.12 | 0.73 | 0.48 | 0.27
Total mag. | 40X | 100X | 200X | 400X | 1000X| 40X | 100X | 200X | 400X | 1000X
34.23 | 17.7 8.99 | 4.67 1.75 | 35.99 | 18.98 | 8.03 | 4.13 | 1.69
WHK 10X
(Field Focal
Field
Lama
[173
|
276
|
945
|
30
|
068
[1254
221
|
76
|
272
|
068
2)
시
5 2
|1
05
|
02
|
5
2:
la
05
|
0.2
*Immersion objectives
The resolving power and focal depth are obtained with fully opened aperture diaphragm.
Technical terms:
© Working distance: The distance from the cover glass to the nearest point of the
objective.
© Numerical aperture: The N.A. represents a performance number which can be com-
pared to the relative aperture (f-number) of a camera lens. The
N.A. values can be used for directly comparing the resolving
powers of all types of objectives. The larger the N.A., the higher
resolving power.
© Resolving power: The ability of a lens to register small details. The resolving power
of a lens is measured by its ability to separate two points.
© Focal depth: The distance between the upper and lower limits of sharpness in
the image formed by an optical system. As you stop down the
aperture iris diaphragm, the focal depth becomes larger. The
larger the N.A. of an objective the shallower the focal depth.
© Field number: A number that represents the diameter in mm of the image of
the field diaphragm that is formed by the lens in front of it.
© Field of view diameter: The actual size of the field of view in mm on the object surface.

一 一 一 一
Vil. TROUBLESHOOTING
If you are unable to obtain full performance from your microscope, please consult with the
table below as pointers for troubleshooting.
Phenomenon
Cause Remedy
1. Optical System
a) With illuminator switched
on, the field of view is
dark.
Field iris diaphragm is not
opened sufficiently.
Open diaphragm to proper
diameter.
Condenser is
much,
lowered too Adjust condenser height.
Light path selector lever is
pulled out to C position.
Push in lever up to CV or V
Position.
b) Field of view is cut off or
illuminated irregularly.
Light path selector lever is
stopped midway.
Click it into proper position
according to your purpose.
Nosepiece is not clicked into
place.
Slightly rotate nosepiece until
it clicks into place.
Nosepiece
mounted,
is not correctly Insert nosepiece dovetail into
microscope frame all the way,
then lock.
The power of objective used
exceeds the illumination ca-
pacity of condenser.
Choose a condenser to meet
your purpose.
Condenser is not centered. Center condenser.
Field iris diaphragm is stopped
down excessively.
Open diaphragm
diameter.
to proper
c) Dust or dirt is visible in
the field of view.
Dust, etc. on light exit lens.
Dust on condenser top lens.
Dirty specimen.
Dust on eyepiece.
Remove dust, etc.
Clean front lenses,
d) Excessive image contrast.
Condenser is lowered too | Adjust condenser height.
much.
Aperture iris diaphragm is | Open diaphragm to proper
stopped down excessively. diameter.

Phenomenon Cause Remedy
e) Resolution problems:
e Image is not sharp.
e Insufficient contrast,
® Image details lack defi: |
nition.
|
Non Olympus objectives are
used.
Use Olympus LB series objec-
tives.
Nosepiece is not correctly | Insert nosepiece dovetail into
mounted, microscope frame all the way,
then lock.
Objective is not correctly | Click nosepiece into place,
positioned in the light path.
Objective correction collar is
not adjusted.
Rotate correction collar, keep-
ing specimen in fine focus
until optimum resolution is
obtained.
Dust on objective front lens. Clean front lens.
Immersion objective is not
used with immersion oil.
Use immersion oil.
Bubbles in immersion oil. Remove bubbles (and reap-
ply oil).
Immersion oil designated by
Olympus is not used,
Use Olympus immersion oil.
Dirty specimens.
Dust on condenser lens.
Clean.
f) Field of view is partially
out of focus, or image is
partly out of focus.
Nosepiece is not correctly | Insert nosepiece dovetail into
mounted, microscope frame all the way,
then lock.
Objective is not correctly | Slightly rotate nosepiece until
positioned in the light path. it clicks in place.
Specimen is not correctly
positioned on stage.
Place specimen slide correctly
on stage, and place stage clips
Open it.
Specimen image is partial-
ly out of focus.
Nosepiece is not correctly | Insert nosepiece dovetail into
mounted. microscope frame all the way,
then lock.
Objective is not correctly | Slightly rotate nosepiece until
Positioned in the light path. it clicks into place.
Condenser is not centered.
Center condenser.
Field of view becomes
only slightly brighter by
increasing voltage.
Condenser
centered.
is not correctly Center condenser.
Condenser is lowered
much.
too
Adjust condenser height.
. Electric System
Illuminator is too bright
(or too dark) even when
adjusting control lever.
Voltage for illuminator
cannot be raised,
Line voltage selector switch
is not matched with local
mains voltage.
Match selector switch to
mains voltage.
18