Olympus FHT-521 User manual

OLYMPUS RESEARCH MICROSCOPES
INSTRUCTION MANUAL
MODELS FHT&EHTWITH§W:

INSTRUCTION MANUAL
OLYMPUS RESEARCH MICROSCOPES
MODELS
FHT&EHT:~~:~~
TABLE OF CONTENTS
I. STANDARD EQUIPMENT " 2
II. SPECiFiCATiONS 4
Ill. IDENTIFICATION OF VARIOUS COMPONENTS 5
IV. DESCRIPTION OF EACH COMPONENT 6
V. OPERATING THE MICROSCOPE
14
VI. OPTICAL CHARACTERiSTICS ...............•...... ·19

I
STANDARD EQUIPMENT
Before assembly, please check your standard outfit, which consists of
the following items:
1. Model FHT
, ) Binocular Tube Versions
Con- Total
Model No. Eyepieces Objectives Stage denser magni-
fication
Bi P7x, Bi WF lOx, Ach
4x,Ach lOx,
N.A. 28x-
FHT-521 Bi P15x, paired Ach 40x. 1.25 1500x
Ach l00x (oill
Bi P7x, Bi High-Eye- Ach
4x.Ach lOx, FrS
N.A. 28x-
FHT-522 pointWF 10x,Bi P15x, FI 40x, FI 100x ISquare 1.40 2000x
Bi
K20x. paired
loill
mechani·
Bi P7x, Bi High-Eye- Plan 4x. Plan lOx,
cal stage
with left N.A. 28x-
FHT-523 pointWF lOx. BiP15x. Plan 40x,
&right 1.40 2000x
Bi
K20x, paired Plan loox loill
hand
coaxial N.A.
Plan4x,Plan lOx.
controls) 0.85
FHT-523- Bi SW10x, paired Plan
20x, Plan40x with
40x-
SW SW
Plan looxloill
aux. con
l000x
denser
lens
2} Trinocular Tube Versions
Con- Total
Model No. Eyepieces Objectives Stage
denser
magni-
fication
Bi P7x, BiWF10x,
Bi P15x. paired
Ach 4x, Ach lOx,
N.A. 28x-
FHT-531 Photo eyepieces: Ach 40x,
1.25 1500x
FK2.5x, FK3.3x Ach 100x (oill
FK5x,FK6.7x, leach
Bi P7x, Bi High-Eye- FrS
point WF10x, Bi P15x,
Ach4x,Ach lOx, (Square
FHT-532 Bi
K20x, paired
FI40x,Fll00x
mechani-
N.A. 28x-
Photo eyepieces:
loill
cal stage 1.40 2000x
FK2.5x, FK3.3x.
with left
FK5x,FK6.7x,l each &right
Bi P7x, Bi High-Eye- hand
coaxial
point WF lOx, Bi P15x, Plan4x, Plan lOx. controls)
FHT-533 Bi
K20x. paired Plan
40x,
N.A.
28x-
Photo eyepieces: Plan
l00x loill
1.40 2000x
FK2.5x, FK3.3x,
FK5x,FK6.7x,l each
N.A.
SW10x paired
Plan4x,Planl0x,
0.85
FHT-533-
Photo eyepieces: Plan20x.Plan40x, with 40x-
SW
FK2.5x, FK3.3x,
SWPlan looxloill
aux. con· looox
FK5x,FK6.7x,l each denser
lens.
Other items supplied with each version:
Spare Bulbs,6V.5A,
TB-' _. _. .. 2pcs.
Filter(blue) ..... .................. .. , pc.
Filter Mount (provided only with achromatic/aplanatic condenser) 1pc.
Wooden Carrying Case. . 1
pc.
Certificate . . . . . 1 pc.
2

2. Model EHT
, ) Binocular Tube Version
Can· Total
Model No. Eyepieces Objectives Stage denser magni-
fication
Bi P7x, Bi WF10x.
Ach4x, Ach lOx.
N.A. 28x-
EHT -421 Ach 40x.
8i P15x, paired Ach
loox (oill
1.25 1500x
CrS·VH
Bi P7x,
BI High·Eye· Ach4x. Ach lOx ISquare N.A.
28x-
EHT-422 pointWFl Ox. Bi Pl 5x. FI 40x. FI loox
mechani- 1.40 2000x
Bi
K20x. paired loill cal stage
Bi P7x, Bi High·Eye- Plan 4x. Plan lOx.
with co-
axial low N.A. 28x-
EHT -423
pointWF 1Ox.Bi Pl 5x. Plan 40x.
drive 1.40 2000x
Bi
K20x. paired Plan loox loill
controls) N.A.
Plan
4x. Plan lOx.
0.85
EHT -423· with
40x-
SW
Bi SW10x. paired Plan20x.Plan40x, aux.
con·
looox
SWPlan looxloill
denser
lens
2) Trinocular Tube Version
Con- Total
Model No. Eyepieces Objectives Stage denser magni·
fication
Bi P7x. Bi WF lOx,
Bi P15x. paired. Ach
4x. Ach lOx,
N.A.
28x-
EHT -431 Photo eyepieces :
Ach40x,Achloox
1.25 1500x
FK2.5x,FK3.3x,
(oill
FK5x,FK6.7x,1 each
Bi P7x. Bi High·Eye-
pointWF10x, Bi P15x,
Ach4x,Ach lOx,
CrS·VH
EHT-432 Bi
K20x, paired. FI
40x. FI loox
(Square
N.A. 28x-
Photo eyepieces:
(oill
mechani- 1.40
2000x
FK2.5x, FK3.3x,
cal stage
FK5x, FK6.7x, 1each
with low
Bi P7x, Bi High·Eye- drive
controls)
pointWF10x, Bi Pl 5x, Plan
4x, Plan lOx,
EHT-433
Bi K20x, paired. Plan
40x,
N.A. 28x-
Photo eyepieces :Plan
loox (oil)
1.40 2000-
FK2.5x, FK3.3x,
FK5x, FK6.7x,l each
N.A.
Bi SW lOx, paired. Plan4x, Plan
lOx,
0.85
EHT·433· Photo eyepieces: Plan
20x ,Plan40x,
with 40x-
SW
FK2.5x, FK3.3x
SWPlan lOOx(oill
aux. con- 1000x
FK5x, FK6.7x,l each
denser
lens
Other items supplied with each version:
Spare
Bulbs,6V,5A.TB·' 2pcs.
Filler(blue) .................................... .. , pc.
Filter Mount(provided only wilh achromatic/aplanalic condenser) 1 pc.
Wooden Carrying Case. 1pc.
Certificate . . . . . . . . 1
pc.
3

II
SPECIFICATIONS
~
--------
FHT EHT
Vertical stage movement. Vertical stage
movement.
Focusing coax
ial coarse and fine ad· separate coarse and fine
justments, with automatic adjustments. with auto-
pre·focusing lever. matic pre-focusing lever.
Coarse
Devetait slideways, rack and pinion type:
adjustment adjustment range 32.5 mm
Fine Adjustment ragne
1.2mm. Adjustment range
1.2mm,
adjustment graduated in increments
of
graduated in increments of
Micra-
1 micron. 2microns.
scope Tungsten lamp, 6V. 5A, with centering device and built·in
Stand Light transformer.
source Auxiliary
lenses for low,
medium and high power
objectives.
Revolving Quintuple, on ball bearings. Engravings. coded Ato E.
nosepiece to facilitate objective insertion.
Condenser With condenser centering device and rack·and-pinion
mount
height adjustment. Height displacement 23.5mm.
Tube inclination 45-, rotatable 360-, both eyepiece tubes
Binocular provided
with diopter and tube length adjustments,
tube except Bi-SW observation tube inclined
30-, and diopter
Obser- difference can be adjusted
by the SW eyepiece tube.
vation I nterpupillary distance adjustment from 56mm to 74mm.
Tubes
Tube inclination 45·, rotatable 360·, both eyepiece tubes
provided with diopter and tube length adjustments, except
Trinocular Tr-SWobservation tube inclined 30·, and diopter difference
tube
can be adjusted by the SW eyepiece tube. Interpupillary
distance adjustment from 56mm to 74mm, with photo
tube. Light path selector lever.
FrS
CrS·VH
Rotatable graduated mech· Rotatable graduated mech·
Stages
anical stage with horizontal anical stage with low drive
drive controls, movement controls, movement range
range 44mm x 76mm. Ver· 52mm x 76mm. Vernier
nier scales reading to O. 1 mm scales reading
to 0.1mm.
Achromatic! NA 1.40, with decenterable aperture iris diaphragm and
aplanatic graduated scale.
condenser
Can·
Abbe N.A. 1.25, with aperture iris diaphragm and swing-out
densers condenser filter
mount.
CO-4
N.A. 0.85, for super widefield of view,
with variable
condenser aperture iris diaphragm, filter holder
and auxiliary
condenser lens.
Photo Eyepieces FK
The new photo eyepieces FKare specially designed for photo-
micrography with the Olympus Photomicrographic System Camera Model
PM-10
(optional). The eyepiece powers available are 2.5x, 3.3x, 5x
and 6.7x. Each magnification is computed to focus an image at aprojec-
tion length of 125mm - the same plane as the 35mm film plane, thus
compensating spherical aberration.
The respective magnifications
are engraved on the FKeyepieces:
Total magnification of a picture
when the FK eyepiece is used. is formulated as follows:
Total magnification with 35mm film =Objective power x FK eye-
piece power.
The formula below, however. will be applied in case the camp.ra
adapter with relay lens, model PM-D L, is used for larger format
camera backs:
Total magnification of.large format picture =Objective power x FK
eyepiece power x3.
4

III
IDENTIFICATION OF VARIOUS COMPONENTS
Trinocular Observation Tube
Limb
Voltage
Adjustment
Knob
FHT·533
5
Eyepiece
Revolving
Nosepiece
Objective
5'"",
Conden~r
Voltmeter

IV
DESCRIPTION OF EACH COMPONENT
A. Mic:roMlOpe Sund
1. Limb 8nd Focusi"9 Mech..,ism
The limb is securely attached to the sturdy base and supports the observa-
tion tube, stage, condenser, revolving nosepiece and focusing mechanism.
The focusing mechanism includes
the coarse and fine adjustments and an
automatic pre-focusing lever. This lever
is provided to prevent possible
contact between specimen and objective as well as
to simplify coarse
focusing.
The lever is locked after coarse focus has been accomplished.
This prevents further upward travel
of the stage and automatically pro-
vides alimiting
stop if the stage is lowered then raised again. The
automatic pre-focusing lever does not restrict fine focusing.
The filter
mount is plaoed on the light exit of the illuminator base.
Stage Clamping Lever
Co_
Adjustment Knob
Fine
Adjustment Knob
Automatic
Pre-focusing Lever
FHT
Aux iI iory Lens
Shifting Lever
Fillllr Mount
Light Exit
Coane
Adjustment Knob
Fine
Adjustment Knob
2. Condenser
The condenser may be mounted on the condenser mount by first
inserting
the condenser into the condenser mount from below, aligning
the positioning dots on the condenser and condenser mount, and then
clamping with
the clamping screw. Condenser centration can be
accomplished by means
of two centering knobs. Vertical movement of
the condenser can be adjusted by the condenser height adjustment knob.
The condenser has an excellent resolving power,
dry or oil immersion,
from
4x to 100x magnification objectives. When using the 100x
objective,
the distance between condenser and specimen should be
filled with immersion oil.
Note: For use with
the achromaticlaplanatic condenser N.A. 1.40, the
filter
mount is placed on the light exit of the illuminator base, while
either
the Abbe N.A. 1.25 or N.A. 0.85 condenser incorporates its
own filter mount. When the N.A. 0.85 condenser is used for super
widefield observation, keep
the auxiliary condenser lens slipped on the
light exit on the base.
The slide clamping screw permits simultaneous locking of slide and
6

rotation of condenser and the slide lever allows decentering and rotating
the aperture iris diaphragm for obligue illumination.
• Abbe Conden5el", N.A. 1.25
Abbe COI"denser --------
Condenser Clamping
Screw
Centering Knob
Filter Moun't-~--'
• Achromatic/aplanatic Condenser, N.A. 1.4
Achromaticl
aplanatic
Condenser -------
Condenser
Clamping
Screw ----~-.
Centering Knob
Aperture Iris
Diaphragm
Cuntrol Aing-~-r-'
•CD-4 Condenser, N.A. 0.85
Condenser
Condenser
Mount
Aperture Iris
l..::> ... _---- Diaphragm
Control lever
,,~~- Alignment Dots
/Condenser Mount
Slide Clamping
Screw
~ ...... Alignment Dots
----Slide Lever
Condenser MOllnt
Condenser Clamping
Screw
Centering Knob
Filter Mount
Alignment Dots
Aperture Iris
Diaphragm Control
Leve<
7

Lever Position
H
L
Fo,
Photomicrography
4x
lOx Observ.
20x-l00x
Objective For
Observation
3. Microscope Base and· Light Source
1) Microscope
Base
The lamp house is built onto the base. The lamp socket is clamped to
the lamp house with adamping screw. The tight path is selected with
the auxiliary lens shifting lever for high, medium and low magnification
of objective in use.
... __ ..
It is generally recommended to set the lever to the position marked with
"Observ." (equivalent to position L) for brightfield observation with
all the objectives from 4x through 100x. In phase contrast or darkfield
observation, where
in tenser light is required, however, it is recommended
to set it to position M or Haccording to objective magnification as in
case with photomicrography.
Voltage
Adjustment Knob
Grounding terminal
..::::::;::;",::::~-- Line Voltage
Adjustment Screw
oVoltage Adjustment
The
minimum voltage required for the light source can be varied qv
means of the line voltage adjustment screw provided at the back of the
microscope
base in accordance with the line voltage and frequency,
since asilicon controlled rectifier (SeRl
is .adopted in the dimmer
circuitry.
At the bottom of the base is avoltage selector switch, which can be
turned with acoin, to correspond with the voltage of main supply
(11 OV, 120V, 220V or 240Vl. The transformer is built in the base and
switched on and
off with the voltage adjustment knob, which also
controls the bulb voltage from 0
to lOV.
8