Olympus MVX10 User manual

INSTRUCTIONS
MVX10
A X 7 4 4 1
This instruction manual is for the Olympus MVX Research Macro Zoom System Microscope. To
ensure the safety, obtain optimum performance and familiarize yourself fully with the use of this
attachment, we recommend you study this manual thoroughly before operating the microscope.
Retain this instruction manual in an easily accessible place near the work desk for future reference.
RESEARCH MACRO ZOOM
SYSTEM MICROSCOPE
MVX10
MVX-2RE
MVX-CA2X
SZX-STAD1
SZH-STAD1
SZX-STAD2
SZ2-FO
SZH-SG
SZH-SC
Main modules described in this manual

MVX10
CONTENTS
IMPORTANT — Be sure to read this section for safe use of the equipment. —
1 NOMENCLATURE
2 CONTROLS
3
4 OPERATION
SUMMARY OF REFLECTED FLUORESCENT LIGHT OBSERVATION PROCEDURE
4-1 Base ....................................................................................................................................................................................................................... 10
4-2 Microscope Body and Focusing Assembly.......................................................................................... 10,11
4-3 Coaxial Fluorescence Illuminator..................................................................................................................... 12-15
4-4 Observation Tube ...................................................................................................................................................................... 15-17
1Using the Stage Plate 2Placing the Specimen
1-4
Correct assembly and adjustments are indispensable for the microscope to manifest its full performance. If you
want to assemble the microscope by yourself, refer to Chapter 11, “ASSEMBLY” before other chapters. (P. 36 to P. 43)
5 TV OBSERVATION AND PHOTOMICROGRAPHY 18,19
5
6,7
8,9
10-17
1Adjusting the Focus
2 Adjusting the Rotation Tension of the Coarse Focus Adjustment Knob
3Engaging and Disengaging the Zooming Knob Click Stop Position
4Adjusting the Aperture Iris Diaphragm 5Using the Objective Correction Collar
1Selecting the Fluorescence Mirror Unit 2Turning the Burner ON
3Opening/Closing the Shutter 4Using the Field Iris Diaphragm
5Switching the Filter Slider Knob
1Adjusting the Tilt 2Adjusting the Interpupillar Distance
3Adjusting the Diopter (Zoom Parfocality Adjustment)
4Using the Eye Shades 5 Mounting the Eyepiece Micrometer Disk
6Selecting the Light Path 7Switching the Monaural/Stereo View
1Selecting the Camera Adapter Magnification
2Attaching the Camera Adapter 3Selecting the TV Camera Light Path
4Adjusting the Parfocality Between Observation Image and Monitor Image

6 TRANSMITTED LIGHT OBSERVATION 20
7 TROUBLESHOOTING GUIDE
8 SPECIFICATIONS
21
22
9 OPTICAL CHARACTERISTICS
PROPER SELECTION OF THE POWER SUPPLY CORD .................................................................. 46,47
23
24-35
10
11
OPERATION OF OTHER MODULES
10-1 Revolving Nosepiece MVX-2RE....................................................................................................................... 24,25
10-2 Magnification Changer MVX-CA2X...................................................................................................................... 26
10-3 BX Stage Adapter Type 1 SZX-STAD1 .................................................................................................... 27-29
10-4 Stage Adapter Type 1 SZH-STAD1 ....................................................................................................................... 29
10-5 BX Stage Adapter Type 2 SZX-STAD2 .................................................................................................... 30,31
10-6 Vertical-Movement Stage SZ2-FO................................................................................................................ 31-33
10-7 Gliding Stage SZH-SG ................................................................................................................................................... 33,34
10-8 Cup Stage SZH-SC............................................................................................................................................................. 34,35
ASSEMBLY 36-45
11-1 Assembly Diagram ...................................................................................................................................................................... 36
11-2 Detailed Assembly Procedures ....................................................................................................................... 37-43
11-3 Centration of the Mercury (Xenon) Burner and Field Iris Diaphragm....... 44,45

1
MVX10
IMPORTANT
SAFETY PRECAUTIONS
1. After observation of a specimen that involves the risk of infection, be sure to clean the positions that contacted the
specimen to prevent infection.
· To avoid the risk that the specimen drops and splatters, be sure to remove the specimen before moving the microscope.
· If a specimen is destroyed due to an erroneous operation, immediately take the infection prevention measures.
· The microscope becomes unstable when its height is increased by a mounted attachment. To prevent the specimen
from dropping if the microscope topples down, be sure to take the toppling prevention measures when mounting an
attachment.
2. The applicable ultrahigh-pressure mercury burners are the USH-103OL (OLYMPUS) and HBO103W/2 (OSRAM), both of
which are DC mercury burners available from Olympus.
3. Ensure that the burners are mounted and that the cords are connected securely.
4. The inside of the lam housing is very hot while the burner is on and immediately after it is turned off. Do not open the lamp
housing in these periods (see page 42).
5. The power supply unit contains high-voltage parts inside. Do not attempt to disassemble it.
6. Always use the power cord provided by Olympus. If no power cord is provided, please select the power cord by referring
to the section “PROPER SELECTION OF THE POWER SUPPLY CORD” at the end of this instruction manual. If the proper
power cord is not used, Olympus can no longer warrant the electrical safety performance of the equipment.
Before plugging the power cord into the wall outlet, ensure that the main switch of the power supply unit is set to “ ”
(OFF).
7. Always ensure that the grounding terminal of the power supply unit is properly grounded. If the equipment is not grounded,
Olympus can no longer warrant the electrical safety performance of the equipment.
8. Before opening the lamp housing for replacing the burner, be sure to set the main switch to “ ” (OFF), unplug the lamp
housing output connector of the power supply unit and wait at least 10 minutes or until the burner and lamp housing
have cooled down.
9. The top of the lamp housing becomes very hot. To avoid the risk of a fire, never block the ventilation of this part.
Make sure you leave a space of 10 cm or more around the lamp housing and power supply unit to dissipate heat.
10. The power cord is also used to shut off the power supply unit in case of emergency by unplugging. To facilitate this, locate
the power supply unit or wall outlet so that the power cord connector (on the rear of the power supply unit) or the wall
outlet is easily accessible in case of emergency.
Safety Symbols
Symbol Explanation
The following symbols are found on the microscope. Study the meaning of the symbols and always use the equipment
in the safest possible manner.
Indicates the presence of a high voltage (1 kV or more), which should not be touched to
prevent an electric shock.
Indicates that the surface becomes hot, and should not be touched with bare hands.
Before use, carefully read the instruction manual. Improper handling could result in injury to
the user and/or damage to the equipment.
Indicates that the main switch is ON.
Indicates that the main switch is OFF.
l

2
Caution indications
Caution indications are affixed at parts where special precaution is required when handling and using the microscope.
Always heed the cautions.
Caution indication
positions
· Lamp housing [High temperature caution]
(U-LH100HG, U-LH10HGAPO)
· Power supply unit [High voltage caution]
}This instruction manual pertains only to the operating procedures of the Research Macro Zoom System. Also read the
instruction manuals for the modules used in combination with the microscope system so that you can understand the
comprehensive operating procedures of the system.
1. A microscope is a precision instrument. Handle it with care and avoid
subjecting it to sudden or severe impact. Also be careful in handling
the limiting or stopper mechanisms because an excessive force may
destroy them.
2. Do not use the microscope where it is subjected to direct sunlight, high
temperature and humidity, or vibration.(For the operating environment,
see Section 8, “SPECIFICATIONS” on page 22.)
3. To prevent the microscope body from turning over, its pivot angle
must be limited to 30° and the tilt angle of the desk surface must
be limited to 5° as shown in Fig.1.
4. Care is required when using an auxiliary pillar (SZH-P400/P600) because
it increases the height of the microscope body and makes it unstable.
5. To adjust the microscope body height, be sure to hold the focusing
assembly with one hand and then loosen the focusing assembly
clamping knob @. (Fig. 1)
(Use the drop prevention collar (SZX-R) to prevent a hazard from
occurring.)
Be careful not to pinch your finger during adjustment.
1Getting Ready
Fig. 1
@

3
MVX10
1. To clean the lenses and other glass components, simply blow dirty away using a commercially available blower and
wipe gently using a piece of cleaning paper (or clean gauze).
If a lens is stained with fingerprints or oil smudges, wipe it gauze slightly moistened with commercially available
absolute alcohol.
Since the absolute alcohol is highly flammable, it must be handled carefully.
Be sure to keep it away from open flames or potential sources of electrical sparks -- for example, electrical
equipment that is being switched on or off.
Also remember to always use it only in a well-ventilated room.
2. The equipment uses plastic resins extensively in its external finish. Do not attempt to use organic solvents to clean the
non-optical components of the microscope. To clean these components, use a lint-free, soft cloth lightly moistened with
a diluted neutral detergent.
3. Never disassemble any part of the microscope as this could result in malfunctions or reduced performance.
4. When not using the microscope, keep it covered with the dust cover provided. Ensure that the lamp housing is cool
before covering the microscope.
5. When the hour counter on the power supply unit indicates 300 hours (USH-103OL, HBO103W/2), set the main switch
to “ ” (OFF) for safety, wait for more than 10 minutes and then replace the burner (see page 42). Unlike the fluorescent
lamps, the mercury burner seals high-pressure gas inside. If it used after the specified service life has been exceeded,
the glass tube may be distorted accumulatively and may eventually burst, though this happens very rarely. The used
mercury burner should be disposed of as an industrial waste. If you cannot dispose of it properly, contact Olympus.
6. When disposing of the microscope. Check the regulations and rules of your local government and be sure to observe
them. To dispose of only the gas spring (|, Fig. 2) used in the counterbalance of the focusing assembly, follow the
precautions provided with the gas spring.
6. Observe the following cautions when operating the coarse focus
adjustment knobs or the zooming knobs.
1. If the knob hits the upper or lower lim-
iting mechanism violently or it is rotated
after it hits a limiting mechanism, the
internal mechanism may be damaged.
Fig. 2
Operation Manipulated
Controls Caution
Focusing Coarse focus
adjustment
knobs @ (Fig. 2)
2. If the knobs on the left and right are
rotated in opposite directions, the in-
ternal mechanism will be damaged.
(The rotation tension of the knob
should be adjusted using the rotation
tension adjustment ring ³ on the
knob. See page 10.)
Zooming knobs
² (Fig. 2)
1. If the knob hits the upper or lower lim-
iting mechanism violently or it is rotated
after it has hit a limiting mechanism,
the internal mechanism may be dam-
aged.
2. If the knobs on the left and right are
rotated in opposite directions, the inter-
nal mechanism will be damaged.
7. Before moving the microscope, detach the modules including the tilting
trinocular head and lamp housing to reduce the total weight. Then hold
it by the base, not by the zoom microscope body.
Zooming
@
²
³
2Maintenance and Storage
|

4
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition,
the microscope may also be damaged. Always use the microscope as outlined in this instruction manual.
3Caution
The following symbols are used to set off text in this instruction manual.
: Indicates that failure to follow the instructions in the warning could result in bodily harm to the
user and/or damage to equipment (including objects in the vicinity of the equipment).
# : Indicates that failure to follow the instructions could result in damage to equipment.
} : Indicates commentary (for ease of operation and maintenance).
4Intended use
This instrument has been designed to be used to observe magnified images of specimens in routine and research
applications.
Do not use this instrument for any purpose other than its intended use.

5
MVX10
1
NOMENCLATURE
}The module names listed below are typical ones that can be used with the system, and the illustration shows the system
composed of modules marked {. Certain other modules are also usable even when they are not mentioned below. For
these modules, refer to the latest catalogues or contact Olympus.
For the modules marked *, refer to their instruction manuals.
Observation Tube
{ Tilting Trinocular Head: MVX-TTRS
· Tube Lens Unit: MVX-TLU
Eyepieces
{ WHN10X-H
Coaxial Fluorescence Illuminator
{ MVX-RFA
<< Fluorescence mirror units >>
· U-MCFPHQ/XL
· U-MGFPHQ/XL
· U-MYFPHQ/XL
· U-MRFPHQ/XL
· U-MGFP/XL
· U-MGFPA/XL
Objective Lens
{ MVPLAPO 1X
· MVPLAPO 0.63X
· MVPLAPO 2XC
Camera Adapter
{ MVX-TV 1XC
· MVX-TV 0.63XC
· MVX-TV1XB
High-Intensity Lamp Housing
· U-LH100HG (for mercury burner)
· U-LH100HGAPO (for mercury burner)
· U-LH75XEAPO*(for xenon burner)
ND Filter
· 32ND6
· 32ND12
· 32ND25
· 32ND50
Focusing Assembly
{ MVX-FOF
Revolving Nosepiece
· MVX-2RE
Base
{ Transmitted Illumination Base : SZX-ILLB2*
· Transmitted Illumination Base : SZX-ILLD2*
· Transmitted Illumination Base : SZX-ILLK*
· Large Base : SZX-STL
Stage Glass & base accessories
{ Stage Glass: SP-C
· Fluorescence Center Plate: SP-FL
· Stage Plate (B&W): SP-BW-2
· Base accessories
Zoom Microscope Body
{ MVX-ZB10
Magnification Changer
· MVX-CA2X
Liquid light guide adapter
{ U-LLGAD

6
}If the microscope is not yet assembled, go to Chapter 11, “ASSEMBLY” (pages 36 to 45).
The following illustration shows the systems with the modules enclosed in . For other ancillary modules, see
subsequent pages.
Tilting Trinocular Head
MVX-TTRS
CONTROLS
Coaxial Fluorescence Illuminator
MVX-RFA
Diopter adjustment ring
(P. 16)
Eyepiece clamping knobs
Mirror unit turret (P. 12)
Mirror unit indicator pocket (P. 38)
Zooming knob (P. 16)
Aperture iris diaphragm knob
(P. 11)
Zoom ratio indicator (P. 11)
Zoom Body
MVX-ZB10
UV shield plate
MVX objective
Camera Adapter
MVX-TV 1XC
Light path selector lever (P. 17)
Mono/stereo selector lever (P. 17)
Lamp Housing for Mercury Burner
U-LH100HG/U-LH100HGAPO
Burner centering knobs
(P. 44)
Collector lens focusing
knob (P. 44)
Filter slider knob (P. 15)
Field iris diaphragm lever (P. 14)
Coarse focus adjustment knob (P. 10)
Fine focus adjustment knob (P. 10)
Coarse focus adjustment knob rotation
tension adjustment ring (P. 10)
Shutter knob (P. 14)
Transmitted Illumination Base
SZX-ILLB2
(Refer to the separate instruction manual.)
Focusing Assembly
MVX-FOF

7
MVX10
Fluorescence mirror units
U-MCFPHQ/XL
U-MGFPHQ/XL
U-MYFPHQ/XL
U-MRFPHQ/XL
U-MGFP/XL
U-MGFPA/XL
}Up to three fluorescence mirror units can be mounted by placing them in
every other dovetail of the six dovetails of the MVX-RFA.
# Each fluorescence mirror unit is composed of the dichroic mirror,
barrier filter and excitation filter that match a specific fluorescent
protein.
}When fabricating an original fluorescence unit by your own, it is
recommended to use the U-MF/XL mirror unit frame (which does
not incorporate a filter). (P. 39)
Use the blank indicator sheets provided with the illuminator to write the
original mirror unit name.
Tube Lens Unit
MVX-TLU
Camera adapter mount dovetail
Mount dovetail
}For details, see the instruction manual provided
with the U-LH75XEAPO and U-RX-T.
Light source
U-HGLGPS
Light volume
adjustment dial
Lighting display
LED
Power switch
Light guide
entrance
Power cord
connector
I(ON) / (OFF)
}For details, see the instruction manual provided with the U-HGLGPS.
Xenon Lamp Housing
U-LH75XEAPO
Power Supply Unit
U-RX-T
Counter
Reset swich
Lamp swich
Power Supply Unit
U-RFL-T
}For details, see the instruction manual provided
with the U-RFL-T.

8
SUMMARY OF REFLECTED FLUORESCENT LIGHT OBSERVATION PROCEDURE
}For the observation procedures of other methods, see page 20.
3-1 Preparation
1. Set the shutter knob to “{” to close the shutter. (Page 14)
2. Mount the fluorescence mirror unit(s) that match the desired observation. (Page 38)
3. Turn the mirror unit turret to engage the desired fluorescence mirror units in the light path. (Page 12)
4. Turn on the mercury burner and wait until the arc stabilizes (for about 5 to 10 minutes).
(If the burner is not centered, center it.) (Page 14)
3-2 Observation Procedure
(Controls) (Page)
Place the specimen on the base. (P. 10)
Engage the optimum mirror unit for the speci-
men in the light path. @ Mirror unit turret (P. 12)
Set the shutter knob to “¦” to open the shut-
ter, and bring the specimen into focus.
² Shutter knob (P. 14)
³ Coarse/fine focus adjustment knobs (P. 10)
Engage the ND filter in the light
path as required. | ND filter (P. 15)
Adjust the field of view so that it is uniform
and brightest. ƒ Collector lens focusing knob (P. 44)
Adjust the tilt.
Adjust the interpupillar distance.
Adjust the diopter.
… Binocular assembly (P. 15)
… Binocular assembly (P. 16)
† Diopter adjustment ring (P. 16)
Open the field iris diaphragm and adjust the
aperture iris diaphragm.
Set the zooming knob for the desired zoom
ratio and bring the specimen into focus.
‡ Field iris diaphragm lever (P. 14)
Š Aperture iris diaphragm knob (P. 11)
‰ Zooming knob (P. 16)
³ Coarse/fine focus adjustment knobs (P. 10)
START OF OBSERVATION
}To interrupt observation for a short period, close the shutter. ² Shutter knob (P. 14)

9
MVX10
}Make a photocopy of this spread and post it near the microscope for quick reference.
@
²
³
|
ƒ
…
†
‡
Š‰

10
OPERATION
1Using the Stage Plate
4-1 Base
1. When performing reflected light observation, place the stage plate with the white or black side up according to the
specimen.
2. When performing transmitted light observation, use the transparent stage glass.
2Placing the Specimen
Place the specimen on the approximate center of the stage plate (or stage glass). Hold the specimen with the specimen
holder as required.
4-2 Microscope Body and Focusing Assembly
With both the coarse focus adjustment knob @ and fine focus adjust-
ment knob ², rotating the knob in the direction of the arrow lowers the
microscope body.
· Stroke per turn of the coarse focus adjustment knob : 36. 8mm.
· Stroke per turn of the fine focus adjustment knob : 1.5 mm.
1Adjusting the Focus (Fig. 3)
#The rotation tension of the coarse focus adjustment knob can be
adjusted with the rotation tension adjustment ring ³. Do not rotate
the knobs on the left and right in opposite directions, for this will
damage the internal mechanism.
}This adjustment is intended to facilitate the rotation of the knob while
preventing the microscope body from lowering spontaneously. For best
ease of use, it is recommended to adjust the rotation tension slightly
tighter than the level at which spontaneous lowering of the microscope
body occurs.
1. Rotate the rotation tension adjustment ring ³ by inserting the Allen
screwdriver into the hole | on the ring periphery.
Rotating the ring clockwise increases the rotation tension of the coarse
focus adjustment knob, and rotating counterclockwise decreases it.
#If the microscope body falls down by its own weight or the focus
obtained by fine focusing is lost immediately, the rotation tension
adjustment may be too light. In this case, rotate the ring clockwise
to increase the rotation tension.
#If the rotation tension adjustment is too tight, delicate focusing
will be impossible and the knob may be damaged. Particularly,
never rotate the fine focus adjustment knob quickly while its
rotation tension is extremely tight.
2(Fig. 3)
Adjusting the Rotation Tension of the
Coarse Focus Adjustment Knob
Fig. 3
@
²
³
|

11
MVX10
Engaging and Disengaging the
Zooming Knob Click Stop Position
}When the click stop knob is engaged to ON, the click stop function is
engaged for each zoom ratio indicated with the zooming knob. When
the knob is disengaged to OFF, the zoom magnification can be varied
continuously and finely near the click groove.
}Click stop can be provided for the nine zoom ratios from 0.8 to 5. The
click stop is engaged when the microscope leaves the factory.
1. To disengage the click stop function, rotate the click stop ON-OFF screw
@ counterclockwise (by about two turns from the ON position, in the
direction opposite to the arrow) using the Allen screwdriver.
# Do not rotate too much, or the cover may be damaged.
2. To engage the click stop function, rotate the click stop ON-OFF screw @
fully clockwise (in the direction of the arrow) using the Allen screwdriver.
The zooming knob then stops at every position corresponding to the
zoom ratio indicated on the zoom ratio indicator ².
}Adjusting the aperture iris diaphragm improves the insufficiency of
brightness in the peripheral section light at intermediate zoom ratios
as well as the depth of focus.
However, setting the aperture iris diaphragm too narrowly degrades
resolution.
When performing fluorescent light observation, it is recommended to
open the aperture iris diaphragm fully for bright observation.
1. Adjust the aperture iris diaphragm knob @ to the left or right.
Rotating the ring toward the left “¦” opens the aperture; rotating it toward
the right “ ” closes it.. Adjust while monitoring the observed image to
confirm the contrast and focal depth improvement effects.
# Do not close the aperture too much, for this may cause degradation in
resolution and/or insufficiency of brightness in the peripheral section.
Water depth 5 mm
or less
}The MVPLAPO 2XC objective is equipped with a correction collar @,
which corrects the aberration produced by a medium such as water or
plastic container.
When observing the specimen through a liquid or petri dish cover, rotate
the correction collar to find the position that provides the best contrast,
· Correction is possible for aberrations equivalent to a water depth of about
5 mm.
· The effect of the correction collar may be less sensible when the zoom
ratio is low or the aperture iris diaphragm is stopped down.
}When the MVX-2RE revolving nosepiece is used, hold the objective so
that the revolving nosepiece does not displace from the position when
turning the correction collar @.
3(Fig. 4)
4Adjusting the Aperture Iris Diaphragm (Fig. 5)
5Using the Objective Correction Collar (Fig. 6)
Fig. 4
@²
Fig. 6
@
Fig. 5
@

12
}Use the fluorescence mirror unit that is optimum for each purpose of observation.
4-3 Coaxial Fluorescence Illuminator
1Selecting the Fluorescence Mirror Unit
Mirror unit name Dichroic mirror Excitation filter Barrier filter Application
U-MCFPHQ/XL DM450HQ BP425-445HQ BA460-510HQ ECFP
U-MGFPHQ/XL DM485HQ BP460-480HQ BA495-540HQ EGFP
U-MYFPHQ/XL DM505HQ BP490-500HQ BA515-560HQ EYFP
U-MRFPHQ/XL DM565HQ BP535-555HQ BA570-625HQ RFP
U-MGFP/XL DM505 BP460-490 BA510IF EGFP
U-MGFPA/XL BA510-550
Spectral Characteristics of the Filters
1. Filter for fluorescent protein CFP U-MCFPHQ/XL 2. Filter for fluorescent protein GFP U-MGFPHQ/XL
Transmittance %
Wavelength nm
3. Filter for fluorescent protein YFP U-MYFPHQ/XL 4. Filter for fluorescent protein RFP U-MRFPHQ/XL
U-MGFPA/XL:
For dye separation
Transmittance %
Wavelength nm
Transmittance %
Wavelength nm
Transmittance %
Wavelength nm

13
MVX10
5. IB excitation filter (wide band) U-MGFP/XL 6. IB excitation filter (wide band) U-MGFPA/XL
Transmittance %
Wavelength nm
Transmittance %
Wavelength nm

14
2Turning the Burner ON
#The burner should be centered before the first use after it has been
mounted or replaced. (see page 44) When resetting the burner hour
counter, press and hold the reset button until the reading becomes
“0.0”.
Set the main switch of the power supply unit to “ ” (ON). The arc image
of the burner will stabilize in 5 to 10 minutes after the burner is ignited.
}A discharge-type mercury burner may not turn on by the first try due to
the characteristics of the burner. If a burner does not turn on, set the main
switch to “ ” (OFF), wait for 5 to 10 seconds and set the main switch to
“ ” (ON) again.
#To avoid shortening the service life of the burner, do not turn a burner
off for 2 hours after turning it on but use the shutter in this period.
#When turning on a mercury burner that has been turned off, wait for
about 10 minutes after it is turned off, because it cannot be turned
on unless the mercury vapor in the burner has cooled down and
liquefied.
}If the lamp housing is opened while the burner is on, the power supply
stops to ensure safety. In this case, set the main switch to “ ” (OFF) and
wait for more than 10 minutes before retrying to turn it on again. Do not
open the lamp housing unless it has cooled down sufficiently.
3Opening/Closing the Shutter (Fig. 7)
}The shutter can be used to close the shutter temporarily when changing
the mirror unit turret or interrupting observation for a short period.
1. When the shutter knob @ is set to “{”, the shutter is engaged to shut off
the excitation light.
2. When the shutter knob @ is set to “¦”, the shutter is disengaged and
opened.
4Using the Field Iris Diaphragm (Fig. 8)
}The centering of the field iris diaphragm can be adjusted (see page 45).
}Push in or pull out the field iris diaphragm lever @ as required to prevent
color fading due to fluorescence in parts other than the observed region.
Fig. 7
@
Fig. 8
@

15
MVX10
5Switching the Filter Slider Knob (Fig. 9)
}Hold the filter slider knob @ of coaxial fluorescence illuminator and move
it toward the left or right to select one of the two filter pockets or idle
position.
The idle position is selected when the knob is pulled to the rightmost
stop position. Pushing the knob at the click position one step after the
idle position selects filter pocket 1, and pushing it further to the leftmost
stop position selects filter pocket 2.
To adjust the brightness using filters, mount the required ND filter
(32ND6, 32ND12, 32ND25, 32ND50) or frost filter (32FR80) in the filter
pockets and select the desired filter pocket with the filter slider knob.
When the mercury burner has been turned on for an extended
period, the metallic part of the filter slider knob and the ND filter
get very hot. Be careful when switching the filter slider knob or
changing the filter.
4-4 Observation Tube
1Adjusting the Tilt (Fig. 10)
}Adjust the height and tilt of the eyepieces of the binocular assembly to
the most comfortable viewing position.
Holding the binocular assembly with both hands, raise or lower it to the
desired position.
# Do not attempt to force the binocular assembly past the upper or
lower stop positions. Applying excessive force could destroy the
mechanism.
Fig. 9
@
Fig. 10

16
2Adjusting the Interpupillar Distance (Fig. 11)
#Be sure to hold the binocular assembly @ with both hands to
make this adjustment.
While looking through the eyepieces, hold the left and right of the bin-
ocular assembly @ and adjust by opening or closing it for binocular
vision until the left and right fields of view coincide completely.
}Make sure that the eyepiece clamping knobs @ are tightened. Adjust-
ment of the diopter according to the user’s eyesight ensures parfocality
even when the zoom magnification is changed.
1. Looking into the right eyepiece, rotate the diopter adjustment ring ² so
that the outer periphery of the field of view is as sharp as possible.
2. Place an easy-to-observe specimen on the stage.
3. Rotate the zooming knob ³ to a low zoom ratio and focus the specimen
using the coarse and fine focus adjustment knobs.
4. Rotate the zooming knob ³ to the highest zoom ratio and focus the
specimen using the coarse and fine focus adjustment knobs.
5. Rotate the zooming knob ³ to the lowest zoom ratio, then focus the
specimen by rotating the left and right diopter adjustment rings |
instead of the coarse and fine focus adjustment knobs.
When Using the Micrometer Eyepiece
1. Look into the micrometer eyepiece and focus on the micrometer scale
by rotating the diopter adjustment rings ².
2. Place an easy-to-observe specimen on the stage.
3. Rotate the zooming knob ³ to a low zoom ratio and focus on the
specimen looking into the micrometer eyepiece and using the coarse
and fine focus adjustment knobs.
4. Rotate the zooming knob ³ to the highest zoom ratio and focus on the
specimen using the coarse and fine focus adjustment knobs.
5. Rotate the zooming knob ³ to the lowest zoom ratio, then focus the
specimen by rotating only the diopter adjustment ring | of the non-
micrometer eyepiece instead of the coarse and fine focus adjustment
knobs.
}Note (or memorize) the diopter readings of the left and right eyepiece
scales so that they can be duplicated quickly in the next observation.
3(Fig. 12)
Adjusting the Diopter
(Zoom Parfocality Adjustment)
Fig. 11
@
Fig. 12
@
²
³
|