Olympus BX53-P User manual

INSTRUCTIONS
This instruction manual is for the Olympus Polarizing Microscope. To obtain optimum performance and
to familiarize yourself fully with the use of your microscope, we recommend that you read this manual
together with the instruction manual for the BX53/51/41 microscope thoroughly before use. Retain this
instruction manual in an easily accessible place near the work desk for future reference.
A X 7 9 8 8
BX53/51/41-P
POLARIZING MICROSCOPE

IMPORTANT — Be sure to read this chapter for safe use of the equipment. — 1
BX53/51/41-P
CONTENTS
2-1 Assembly Diagram ......................................................................................................................................................................................................................................... 3
2-2 Detailed Assembly Procedure ................................................................................................................................................................................................ 4-9
4-1 Stage ......................................................................................................................................................................................................................................................................... 12 -14
4-2 Intermediate Polarizing Attachment...................................................................................................................................................................................... 15
5-2 Orthoscopic Observation .....................................................................................................................................................................................................................25
5-3 Conoscopic Observation ...................................................................................................................................................................................................................... 26
1 Adjusting the Optical Axis .......................................... 16-21 2 Adjusting for Extinction ............................................................. 22
3Adjusting the Eyepiece Cross Lines .................................................................................................................................................................................... 23,24
5-1 Adjustments Before Observation .................................................................................................................................................................................... 16-24
1 NOMENCLATURE 2
2 ASSEMBLY 3-9
3 CONTROLS 10-11
4 USING THE CONTROLS 12-15
5 POLARIZED LIGHT OBSERVATION 16-26
6 SPECIFICATIONS 27-28
7 OPTICAL CHARACTERISTICS 29
8 TROUBLESHOOTING GUIDE 30

1
IMPORTANT
1. A microscope is a precision instrument. Handle it with care and avoid subjecting it to sudden or severe impact.
2. The BX53/BX51/BX41 microscope can be used with up to two additional intermediate attachments (e.g., U-DO3 dual-
viewing attachment, U-CA or U-ECA magnification changer, etc.). When using an additional intermediate attachment,
please make sure with your Olympus representative or the latest brochure.
3. Do not use the microscope where it is subjected to direct sunlight, high temperature and humidity, dust or vibrations.
Install it on stable, level bench. For operation environment conditions, see SPECIFICATIONS on page 28.
4. When disposing of the microscope, check the regulations and rules of your local government and be sure to observe them.
If the microscope is used in a manner not specified by this manual, the safety of the user may be imperiled. In addition, the
equipment may also be damaged. Always use the equipment as outlined in this instruction manual.
1Getting Ready
2Caution
The following symbols are used to set off text in this instruction manual.
: Indicates a potentially hazardous situation which, if not avoided, may result in minor
or moderate injury or damage to the equipment or other property. It may also be used
to alert against unsafe praclices.
} : Indicates commentary (for ease of operation and maintenance).
CAUTION
The BX53/51-P and BX41-P have different combinations of microscope frames and stages.
Module BX53/51-P BX41-P
Microscope frame BX53F/BX51TF BX41TF
Stage U-SRP U-SRG2

2
BX53/51/41-P
NOMENCLATURE
Eyepiece
*Polarizing intermediate
attachment
*Centerable revolving
eyepiece
*Polarizing rotatable
stage, or
Rotatable stage
Observation tube
*Objective
(Strain-free objective series)
Lamp housing
*Components for polarized light observation
*Strain-free
condenser
BX53/51 Microscope
frame, or
BX41Microscope frame

3
ASSEMBLY
The diagram below shows how to assemble the various components. The numbers indicate the order of assembly.
}For details on the BX53/51/41 microscope frame, consult the BX53/51/41 manual.
2-1 Assembly Diagram
Eyepiece
Centerable revolving
nosepiece (U-P4RE)
Test plate adapter
(U-TAD)
Sensitive tint plate
(U-TP530)
Quarter wave plate
(U-TP137)
Strain-free
objective
Mechanical stage
(U-FMP)
Stage clips
Polarizing
rotatable stage
(U-SRP)
Rotatable stage
(U-SRG2)
Polarizing
intermediate
attachment
(U-CPA)
Observation tube
Intermediate attachment
for AN360P (U-OPA)
Rotatable analyzer
(U-AN360P-2)
Power cord
Strain-free condenser
(U-POC-2)
*6 types of compensators available
*
CAUTION When assembling the components, make sure that all parts are free of dust and dirt, and avoid scratching
any parts or touching glass surfaces.
100W halogen lamp
housing
100W
halogen
bulb
lamp sooket
Microscope frame
BX53F
BX51TF
BX41TF

4
BX53/51/41-P
Fig. 2
…
2-2 Detailed Assembly Procedure
1Attaching the Stage (U-SRP) (Fig. 1)
1. Turn the coarse adjustment knob @ to lower the substage assembly ² to
its lower limit.
2. Loosen the centering knobs ³.
3. Position the stage with the vernier scale | in front, and carefully lower the
stage onto the round dovetail on the stage bracket ² with the positioning
pin on the stage aligned with the groove on the front of the stage bracket,
then loosely tighten the centering knobs.
ƒ
Fig. 1
@
²
³
|
Mounting Stage Clips and Mechanical Stage (U-FMP) (Fig. 2)
· Insert the stage clips securely into any two holes ƒ on the stage top
surface.
· Mount the mechanical stage so that the positioning pins on the under-
side fit into the positioning holes … on the stage top surface. Using the
Allen screwdriver provided with the microscope frame, tighten the clamping
screw.

5
1. Turn the coarse adjustment knob @ to raise the stage to its upper limit.
2. Turn the condenser height adjustment knob ² to lower the condenser
holder to its lower limit.
3. Loosen the condenser clamping screw ³.
4. Position the condenser with the scale markings in front, insert it into the
substage fork as far as it will go.
Align the positioning pin at the back of the condenser with the groove in
the substage fork.
Swing the top lens out of the way before inserting the condenser.
5. Tighten the condenser clamping screw, then raise the condenser to its
upper limit.
2Mounting the Condenser (U-POC-2) (Fig. 3)
²
Fig. 3
³@
CAUTION

6
BX53/51/41-P
Insert the 10X or 20X objective into the primary hole (position where black
rubber plugs are inserted into the centering holes on the nosepiece).
Mount the other objectives in such a manner, that the magnification in-
creases from low to high power in a clock-wise direction from the primary
hole.
4Mounting the Revolving Nosepiece (U-P4RE) (Fig. 5)
1. Turn the coarse adjustment knob to lower the stage all the way.
2. Using the Allen screwdriver, loosen the nosepiece clamping screw @ on
the microscope frame.
3. Carefully slide the nosepiece along the dovetail to its limit, in the direction
of the arrow.
4. Clamp the nosepiece by tightening the nosepiece clamping screw.
Fig. 4
Low
High
Fig. 5
3Mounting the Objectives (Fig. 4)
@

7
When Using Intermediate Attachment for conoscopic and orthoscopic
observation (U-CPA)
Always make sure to mount this unit in such a manner, that it is
parallel to the arm.
Intermediate Attachment for orthoscopic observation (U-OPA)
}The position of this intermediate tube can be adjusted later. At this point,
place the tube so the nameplate is toward the rear.
1. Using the Allen screwdriver, loosen the observation tube clamping screw
@ on the microscope frame.
2. Insert the circular dovetail mount at the bottom of the intermediate
attachments into the opening on the microscope frame and clamp
by tightening the clamping screw @.
@
Fig. 6
5Mounting the Intermediate Attachments (Fig. 6)
CAUTION