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Olympus SZX16 Installation guide

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RESEARCH STEREOMICROSCOPE SYSTEM
Olympus has acquired ISO9001/ISO14001 certification for its quality/environmental
management systems. In line with this qualification, newly developed SZX16/SZX10
products are manufactured without lead, hexavalent chromium, or cadmium.
Olympus products contribute to society through their environmentally friendly designs.
Specifications are subject to change without any obligation on the part of the manufacturer.
Images are courtesy of the following institutions:
RIKEN Brain Science Institute, Laboratory for Developmental Gene Regulation (page 1, left; page 3,
lower left; pages 5, 7, & 8, lower right). RIKEN Center for Developmental Biology, Laboratory for Cell Asymmetry,
Dr. Ayano Kawaguchi (page 3, lower right). Graduate School of Medicine and Faculty of Medicine,
the University of Tokyo, Department of Cell Biology and Anatomy, Dr. Yasushi Okamoto (page 1, right; page 3,
top; page 5, top right; page 7, top right). National Institute of Advanced Industrial Science and Technology,
Research Institute for Cell Engineering, Neuronics Research Group (page 1, right).
ISO9001
Certification
Design and production
adheres to ISO9001
international quality standard.
ISO14001
Design and production at the OLYMPUS
CORPORATION Ina Plant conforms with
ISO14001 specifications for
environmental management systems.
Certification
008
UKAS
ENVIRONMENTAL
MANAGEMENT
Certified ISO 14001 by
Cutting-edge biological and medical laboratories are environments that require the most
effective imaging and observation of a vast quantity of live specimens. By meeting these
needs, the research stereomicroscope system has advanced. Pursuant to its mission of
“looking into the world of the unknown”, the SZX2 Stereomicroscope Series has been
refined to a higher level of quality and performance that redefines the possible for
stereomicroscopes.
Superb optical performance and maximum comfort have therefore been realized. Image
clarity exceeding that of conventional stereomicroscopes derives from unprecedented high
resolution achieved by the highest available NA and a depth of field perceived as deeper
from multi-wave length astigmatism-free design that absolutely minimizes astigmatism.
SZX2 is ergonomically redesigned especially to reduce fatigue and to provide ultimate
ease of use for long-time observation and other tasks. SZX2 opens the door for you to a
new world, untouched and unseen.
Olympus expertise raises stereomicroscopes to a new level with the world’s highest
zoom ratio* and maximum numerical aperture (NA). Unmatched image clarity and
a highly flexible optical system assure operational ease. The SZX2 Research
Stereomicroscope System reveals a new world, far beyond expectations.
Reaching a new dimension of microscopy —
beyond the imagination.
Reaching a new dimension of microscopy —
beyond the imagination.
P03-P08
A new dimension in image clarity
Unparalleled sharp viewing is the result of
a multi-wavelength, astigmatism- free
design that absolutely minimizes
aberration and the world's highest NA.
From low to high magnification,
unprecedented bright and even
fluorescence observation is achieved.
Long working distance (WD) and high NA
are featured. Overall work efficiency is
assured, including the illumination base
design.
P09-P10
Comfortable operability
Tilting trinocular tube, with an optimum
convergence angle and new slim
illumination base, effectively eliminates
fatigue resulting from long-time
observation and other tasks.
P11-P12
Ergonomic design for working ease
From brightfield to fluorescence
observation, image acquisition of various
specimens is possible at high resolution.
P13-P14
Digital imaging
Accessories for maximizing optical
performance and operability include a
variety of illumination bases, light guides,
and stage plates.
P15-P16
Equipment answering various needs
*Olympus finding, as of March 31, 2006.
Cutting-edge biological and medical laboratories are environments that require the most
effective imaging and observation of a vast quantity of live specimens. By meeting these
needs, the research stereomicroscope system has advanced. Pursuant to its mission of
“looking into the world of the unknown”, the SZX2 Stereomicroscope Series has been
refined to a higher level of quality and performance that redefines the possible for
stereomicroscopes.
Superb optical performance and maximum comfort have therefore been realized. Image
clarity exceeding that of conventional stereomicroscopes derives from unprecedented high
resolution achieved by the highest available NA and a depth of field perceived as deeper
from multi-wave length astigmatism-free design that absolutely minimizes astigmatism.
SZX2 is ergonomically redesigned especially to reduce fatigue and to provide ultimate
ease of use for long-time observation and other tasks. SZX2 opens the door for you to a
new world, untouched and unseen.
Olympus expertise raises stereomicroscopes to a new level with the world’s highest
zoom ratio* and maximum numerical aperture (NA). Unmatched image clarity and
a highly flexible optical system assure operational ease. The SZX2 Research
Stereomicroscope System reveals a new world, far beyond expectations.
Reaching a new dimension of microscopy —
beyond the imagination.
Reaching a new dimension of microscopy —
beyond the imagination.
P03-P08
A new dimension in image clarity
Unparalleled sharp viewing is the result of
a multi-wavelength, astigmatism- free
design that absolutely minimizes
aberration and the world's highest NA.
From low to high magnification,
unprecedented bright and even
fluorescence observation is achieved.
Long working distance (WD) and high NA
are featured. Overall work efficiency is
assured, including the illumination base
design.
P09-P10
Comfortable operability
Tilting trinocular tube, with an optimum
convergence angle and new slim
illumination base, effectively eliminates
fatigue resulting from long-time
observation and other tasks.
P11-P12
Ergonomic design for working ease
From brightfield to fluorescence
observation, image acquisition of various
specimens is possible at high resolution.
P13-P14
Digital imaging
Accessories for maximizing optical
performance and operability include a
variety of illumination bases, light guides,
and stage plates.
P15-P16
Equipment answering various needs
*Olympus finding, as of March 31, 2006.
4
A new dimension in image clarity
A new dimension in image clarity
Olympus’ new SDF objective lenses provide the highest
NA with 900lp/mm resolution. Optimum specimen viewing
from large field overview to microstructure, along with
instant zoom function to select observation points, is assured.
The SZX16 offers optical performance at the highest global standard. SDF objective lenses with the
highest available NA enable microstructural observation that could not be achieved before. And with
the world’s highest zoom range of 7.0x-115x, this all-in-one microscope answers a wide range of needs
related to operation at low magnifications to detailed observation at high magnifications. These
outstanding features meet the need for clear observation of transparent specimens of minimal
contrast to microstructural viewing. Manipulation of live specimens has never been easier.
The world’s highest zoom ratio of 1:16.4 is achieved.
From low to high magnification, several different observation tasks
are available on one microscope.
Model WD (mm) Magnification*
SDFPLFL0.3X 141 2.1x-34.5x
SDFPLAPO0.5XPF 70.5 3.5x-57.5x
SDFPLAPO0.8X 81 5.6x-92x
SDFPLAPO1XPF 60 7x-115x
SDFPLAPO1.6XPF 30 11.2x-184x
SDFPLAPO2XPFC 20 14x-230x
*Using WHN 10X-H
SZX16(withSDFPLAPO2×PFC)
Conventional model
SDF Objective Lens Series
■Highest available NA
The SZX16 realizes the highest NA (0.3) among
stereomicroscopes with 2x objectives. Optical performance
is 30% better than that of comparable products and allows
for significantly more image information.
■
SDF lineup: six objectives for various uses
The broad lineup of the SZX16 PLAN APO objective
series covers several needs with features ranging from
long WD objectives for operations requiring wide
working space (such as sample acquisition or selection)
to high magnification objectives with the world's
highest NA for microstructural observation.
■
World’s highest zoom ratio for several observation tasks
With a zoom range of 7.0x-115x (using SDFPLAPO 1X and
WHN10X-H), the SZX16 has the world’s highest zoom ratio of
1:16.4. From sample verification and selection at low
magnification to microstructure verification at high
magnification, seamless observation is available.
■
Two objectives combine with revolving nosepiece for
3.5x – 230x zoom
The Parfocal (PF) Series of 0.5x, 1x, 1.6x, and 2x comprises
four PF objectives. The revolving nosepiece allows easy
switching between two objective lenses and smooth
zooming between 3.5x and 230x (using WHN10X-H).
4
A new dimension in image clarity
A new dimension in image clarity
Olympus’ new SDF objective lenses provide the highest
NA with 900lp/mm resolution. Optimum specimen viewing
from large field overview to microstructure, along with
instant zoom function to select observation points, is assured.
The SZX16 offers optical performance at the highest global standard. SDF objective lenses with the
highest available NA enable microstructural observation that could not be achieved before. And with
the world’s highest zoom range of 7.0x-115x, this all-in-one microscope answers a wide range of needs
related to operation at low magnifications to detailed observation at high magnifications. These
outstanding features meet the need for clear observation of transparent specimens of minimal
contrast to microstructural viewing. Manipulation of live specimens has never been easier.
The world’s highest zoom ratio of 1:16.4 is achieved.
From low to high magnification, several different observation tasks
are available on one microscope.
Model WD (mm) Magnification*
SDFPLFL0.3X 141 2.1x-34.5x
SDFPLAPO0.5XPF 70.5 3.5x-57.5x
SDFPLAPO0.8X 81 5.6x-92x
SDFPLAPO1XPF 60 7x-115x
SDFPLAPO1.6XPF 30 11.2x-184x
SDFPLAPO2XPFC 20 14x-230x
*Using WHN 10X-H
SZX16(withSDFPLAPO2×PFC)
Conventional model
SDF Objective Lens Series
■Highest available NA
The SZX16 realizes the highest NA (0.3) among
stereomicroscopes with 2x objectives. Optical performance
is 30% better than that of comparable products and allows
for significantly more image information.
■
SDF lineup: six objectives for various uses
The broad lineup of the SZX16 PLAN APO objective
series covers several needs with features ranging from
long WD objectives for operations requiring wide
working space (such as sample acquisition or selection)
to high magnification objectives with the world's
highest NA for microstructural observation.
■
World’s highest zoom ratio for several observation tasks
With a zoom range of 7.0x-115x (using SDFPLAPO 1X and
WHN10X-H), the SZX16 has the world’s highest zoom ratio of
1:16.4. From sample verification and selection at low
magnification to microstructure verification at high
magnification, seamless observation is available.
■
Two objectives combine with revolving nosepiece for
3.5x – 230x zoom
The Parfocal (PF) Series of 0.5x, 1x, 1.6x, and 2x comprises
four PF objectives. The revolving nosepiece allows easy
switching between two objective lenses and smooth
zooming between 3.5x and 230x (using WHN10X-H).
6
5
A new dimension in image clarity
A new dimension in image clarity
Unprecedented depth and sharp images
dramatically enhance efficiency for a variety
of tasks including specimen manipulation.
Multi-wave length, astigmatism-free design for sharp 3D observation.
New standards in image clarity begin with this design.
■Sharp, detailed observation of specimens
Newly designed SDF objective lenses keep astigmatism to a
minimum. This effectively eliminates image deforming at
pre- and post-focal plane and thus the depth of field is
perceived as deeper than before. These design features
enable stress-free use of forceps in the field of view during
live sample selection and acquisition. SZX2 puts power into
action for long-time observation. When these objectives are
combined with the newly developed transmitted light
illumination base, clear observation is even possible for
transparent specimens where contrast is low. Oversights are
thus minimized for specimen selection, dissection, and
manipulation.
■Optical performance with less fatigue
A 360º view of balanced images is made possible by
accommodating vertical and horizontal parameters.
Discomfort in the eyes and body, as well as stress from
prolonged observation or operation, is effectively eliminated.
■
Integration of apochromatic system
The apochromatic system — integrated into observation tubes,
zoom body, and objectives — eliminates chromatic aberration
throughout the zoom range and ensure excellent image quality
without chromatic blur.
■
SZX16: new optics for equally easy handling of thick specimens
Obtaining a lucid visual perspective, such as a clear feel for dimension, is essential for manipulation of thick specimens like
eggs or embryos. The latest proprietary SZX16 delivers the images needed for observation of such specimens. 3D observation
images from surface to interior are also particularly effective in the dissection and manipulation of live specimens.
Non astigmatism-
free design SDF objective
lenses
Pre-focal
plane
Focal plane
Post-focal
plane
Astigmatism effects greater
deforming of the image at
pre- and post-focal plane.
As deforming of the image at
pre- and post-focal plane is
kept to a minimum, the eye can
focus around the point the user
wants to observe. Thus depth
of field is perceived as deeper.
•
Depth of field seen in focal plane will vary according to individual differences in users’ vision.
Previous model
“Multi-wave length astigmatism-free”: a new design effectively eliminating image-deforming
aberration and enabling remarkably sharp 3D imaging and dramatically enhanced specimen
manipulation. Also, with an apochromatic lens system that effectively reduces chromatic aberration,
the latest proprietary SZX 16 optical system provides sharp 3D observation images of various
specimens.
6
5
A new dimension in image clarity
A new dimension in image clarity
Unprecedented depth and sharp images
dramatically enhance efficiency for a variety
of tasks including specimen manipulation.
Multi-wave length, astigmatism-free design for sharp 3D observation.
New standards in image clarity begin with this design.
■Sharp, detailed observation of specimens
Newly designed SDF objective lenses keep astigmatism to a
minimum. This effectively eliminates image deforming at
pre- and post-focal plane and thus the depth of field is
perceived as deeper than before. These design features
enable stress-free use of forceps in the field of view during
live sample selection and acquisition. SZX2 puts power into
action for long-time observation. When these objectives are
combined with the newly developed transmitted light
illumination base, clear observation is even possible for
transparent specimens where contrast is low. Oversights are
thus minimized for specimen selection, dissection, and
manipulation.
■Optical performance with less fatigue
A 360º view of balanced images is made possible by
accommodating vertical and horizontal parameters.
Discomfort in the eyes and body, as well as stress from
prolonged observation or operation, is effectively eliminated.
■
Integration of apochromatic system
The apochromatic system — integrated into observation tubes,
zoom body, and objectives — eliminates chromatic aberration
throughout the zoom range and ensure excellent image quality
without chromatic blur.
■
SZX16: new optics for equally easy handling of thick specimens
Obtaining a lucid visual perspective, such as a clear feel for dimension, is essential for manipulation of thick specimens like
eggs or embryos. The latest proprietary SZX16 delivers the images needed for observation of such specimens. 3D observation
images from surface to interior are also particularly effective in the dissection and manipulation of live specimens.
Non astigmatism-
free design SDF objective
lenses
Pre-focal
plane
Focal plane
Post-focal
plane
Astigmatism effects greater
deforming of the image at
pre- and post-focal plane.
As deforming of the image at
pre- and post-focal plane is
kept to a minimum, the eye can
focus around the point the user
wants to observe. Thus depth
of field is perceived as deeper.
•
Depth of field seen in focal plane will vary according to individual differences in users’ vision.
Previous model
“Multi-wave length astigmatism-free”: a new design effectively eliminating image-deforming
aberration and enabling remarkably sharp 3D imaging and dramatically enhanced specimen
manipulation. Also, with an apochromatic lens system that effectively reduces chromatic aberration,
the latest proprietary SZX 16 optical system provides sharp 3D observation images of various
specimens.
8
7
A new dimension in image clarity
A new dimension in image clarity
From low to high magnification, exceptionally
bright and even fluorescence is assured for
efficient screening.
Newly designed SDF objective lenses of the highest NA and Olympus’
newly developed near-vertical reflected light illuminator will
significantly improve signal intensity and support bright fluorescence
observation.
■
Highest NA among stereomicroscopes provides
extraordinarily bright fluorescence observation
SDF objective lenses of the highest NA dramatically
improve fluorescence detection sensitivity. Furthermore,
the newly designed near-vertical reflected light illuminator’s
excitation light paths are independent from the observation
paths, allowing for substantially improved excitation light
efficiency. These features provide far brighter fluorescence
observation than conventional stereomicroscopes at all
magnifications. Transmitted light observation for verification
of specimen outline is possible even under reflected light
fluorescence observation.
■Five-position turret with nine-filter selection
Nine filter units, ranging from UV excitation to RFP, respond to
applications using various fluorescent dyes and protein. Olympus
High Quality (HQ) filters have an edge steepness and high
transmission that efficiently detect the light of fluorescence to
enhance and capture brighter fluorescence images in precise
detail.
■
Even and seamless fluorescence observation from
low to high magnification
In correspondence with zoom function, the reflected light
illuminator provides even illumination from low to high
magnification. This will eliminate oversights along the periphery
of the field of view during live specimen screening and
dramatically improve work efficiency.
Bright fluorescence observation is essential in recent biological and medical research. Observation of
weak fluorescence at low magnification under stereomicroscope has especially been a problem.
However, the SZX16 enables even and overwhelmingly bright fluorescence observation from low to
high magnifications.
Filter unit Model Remarks
For UV excitation SZX2-FUV Ex330-385/Em420-
For BV excitation SZX2-FBV Ex400-440/Em475-
High performance for CFP SZX2-FCFPHQ Ex425-445/Em460-510
For GFP SZX2-FGFP Ex460-490/Em510-
For GFP separation SZX2-FGFPA Ex460-495/Em510-550
High performance for GFP SZX2-FGFPHQ Ex460-480/Em495-540
High performance for YFP SZX2-FYFPHQ Ex490-500/Em510-560
For RFP 1 SZX2-FRFP1 Ex530-550/Em575-
For RFP 2 SZX2-FRFP2 Ex540-580/Em610-
Fluorescence and transmitted light illuminationFluorescence illumination only
SZX16 reflected light fluorescence illumination stand
SZX16 Fluorescent filter unit
8
7
A new dimension in image clarity
A new dimension in image clarity
From low to high magnification, exceptionally
bright and even fluorescence is assured for
efficient screening.
Newly designed SDF objective lenses of the highest NA and Olympus’
newly developed near-vertical reflected light illuminator will
significantly improve signal intensity and support bright fluorescence
observation.
■
Highest NA among stereomicroscopes provides
extraordinarily bright fluorescence observation
SDF objective lenses of the highest NA dramatically
improve fluorescence detection sensitivity. Furthermore,
the newly designed near-vertical reflected light illuminator’s
excitation light paths are independent from the observation
paths, allowing for substantially improved excitation light
efficiency. These features provide far brighter fluorescence
observation than conventional stereomicroscopes at all
magnifications. Transmitted light observation for verification
of specimen outline is possible even under reflected light
fluorescence observation.
■Five-position turret with nine-filter selection
Nine filter units, ranging from UV excitation to RFP, respond to
applications using various fluorescent dyes and protein. Olympus
High Quality (HQ) filters have an edge steepness and high
transmission that efficiently detect the light of fluorescence to
enhance and capture brighter fluorescence images in precise
detail.
■
Even and seamless fluorescence observation from
low to high magnification
In correspondence with zoom function, the reflected light
illuminator provides even illumination from low to high
magnification. This will eliminate oversights along the periphery
of the field of view during live specimen screening and
dramatically improve work efficiency.
Bright fluorescence observation is essential in recent biological and medical research. Observation of
weak fluorescence at low magnification under stereomicroscope has especially been a problem.
However, the SZX16 enables even and overwhelmingly bright fluorescence observation from low to
high magnifications.
Filter unit Model Remarks
For UV excitation SZX2-FUV Ex330-385/Em420-
For BV excitation SZX2-FBV Ex400-440/Em475-
High performance for CFP SZX2-FCFPHQ Ex425-445/Em460-510
For GFP SZX2-FGFP Ex460-490/Em510-
For GFP separation SZX2-FGFPA Ex460-495/Em510-550
High performance for GFP SZX2-FGFPHQ Ex460-480/Em495-540
High performance for YFP SZX2-FYFPHQ Ex490-500/Em510-560
For RFP 1 SZX2-FRFP1 Ex530-550/Em575-
For RFP 2 SZX2-FRFP2 Ex540-580/Em610-
Fluorescence and transmitted light illuminationFluorescence illumination only
SZX16 reflected light fluorescence illumination stand
SZX16 Fluorescent filter unit
910
Effective combination of long WD and high NA.
Development of an ergonomic design.
Design focus on creating a wide working space and comfort.
Comfortable operability Comfortable operability
■Slim 41mm LED illumination base
■Ergonomic design for user-friendly base
■Wide working space and high NA
SZX2 responds well to a variety of specimens and operations ―from large specimens like mice to small ones like zebra
fish, nematode or drosophilia eggs ―because of the effective combination of high numerical aperture and wide working
space. Moreover, the transmitted light illumination base is newly designed thin (only 41mm) to provide a wide working
space and allow various users to work comfortably.
Operability configured as a whole-system characteristic. Design is focused
not only on WD and NA, but also on a stage that is slim.
Brightfield/darkfield transmitted light illumination
base (SZX2-ILLD)
High-level transmitted light
illumination base (SZX2-ILLB)
Slim LED transmitted light illumination base (SZX2-ILLT)
2x objectives with ease of access and correction collar
WD60mm and NA0.15 from the 1x objective
With a slim design of 41mm — approximately half that of conventional models
— the SZX16 transmitted light illumination base has a lower height to enable
a low eyepoint and easy access to base-mounted samples during observation
and operation. The world’s first LED 4-position turret enables contrast
adjustment between brightfield, oblique, and darkfield illumination with a
simple turn. This makes the SZX16 the all-in-one microscope for various
samples and observation tasks. Another advantage of LED illumination is
keeping down the temperature of the base surface, which is suitable for long-
time manipulation of live specimens. Power consumption is about half that of
a conventional 30W Halogen light source. A life cycle of over 10,000 hours
significantly reduces operation costs.
This unit provides effective contrast from
oblique illumination and easily selected
“High” and “low” contrast settings. Light
volume and color temperature are adjusted by
means of built-in filters (LBD/ND). It also has a
cooling fan to prevent overheating of the base
surface.
The 1x objective has a WD (60mm) that gives the user room to move
and an NA (0.15) that meets the needs of advanced research. Also
available are 0.8x objectives that have a longer WD of 81mm, which
provides not only a larger working space between objective lenses and
sample but also a total magnification of 5.6x-92x (using WHN10x-H).
Offering a wide working space in which users can place several Petri dishes, these illumination bases have an ergonomic,
beveled design for users to work comfortably and naturally.
A highly flexible design assures a wide access angle to objectives with the
highest NA (0.3) for specimen selection. An additional correction collar can
adjust image quality independently of the specimen — a first in
stereomicroscopes.
Transmitted light illumination base (SZX2-ILLK)
This base enables darkfield observation under illumination
twice as bright as conventional models. Flat and thin
specimens like brain tissue slices are vividly displayed on a
black background. A cooling fan prevents the illumination base
surface from overheating.
Offering outstanding cost-efficient performance, this
illumination base uses oblique illumination to provide high-
contrast images of transparent specimens.
NA
0.15
WD
60mm
Field of view
31.4mm