Opentrons Thermocycler Module GEN2 User manual
Thermocycler GEN2
Instruction Manual
Opentrons Labworks Inc.
Edition
GEN2, January 2023
–SafeUseSpecications
–SafetyWarningLabels
–InstrumentSafetyWarnings
–StandardsCompliance
Table of Contents
Product and Manufacturer Description 3
Safety Information and Regulatory Compliance 4
–ProductDescription
–ManufacturerDescription
–ModelNumber
–IncludedParts
–PhysicalSpecications
–TemperatureProle
–LEDStatusLight
Product Specications 11
–VentilationRequirements
–SetupSteps
–PlateLatching
–EmergencyLidOpening
Setup 13
–Cleaning
–Repair
–Warranty
Maintenance 20
Étiquettes d’avertissement de sécurité 21
3
Product and Manufacturer Description
PRODUCT DESCRIPTION
TheOpentronsThermocyclerModuleGEN2provideson-deck,fully
automatedthermocycling.Themoduleenablesautomationof
upstreamanddownstreamworkowsteps.
MANUFACTURER DESCRIPTION
Opentrons Labworks Inc
45-18CtSquareW
LongIslandCity,NY11101
THERMOCYCLER MODULE GEN2
Safety Information and Regulatory Compliance
Opentronsstronglyrecommendsthatyoufollowthesafeuse
specicationslistedinthissectionandthroughoutthismanual.
Note: The Thermocycler heats and cools very quickly during
operation.
SAFE USE SPECIFICATIONS
Input and Output Connections
TheThermocyclerhasthefollowingpowerinputrequirements,which
aremetbytheincludedpowersupply.
Warning:Donotreplacethepowersupplycableunlessatthe
directionofOpentronsSupport.
PowerSupply(AC):
◾Voltage:100–240V
◾Frequency: 50/60 Hz
◾Current:8.5–5A
◾Overvoltage:CategoryII
5
Safety Information and Regulatory Compliance
Environmental Conditions
TheThermocyclershouldonlybeusedindoors,onasturdy,dry,
athorizontalsurface.TheThermocyclermustbeinstalledinalow-
vibrationenvironmentwithstableambientconditions(e.g.,keepthe
ThermocyclerawayfromdirectsunlightorHVACsystemsthatmay
causesignicanttemperatureorhumiditychanges).
Environmentalconditionsforrecommendeduse,acceptableuse,and
storagevary:
Recommended
for System
Operation
Acceptable for
System Operation
Storage and
Transportation
Ambient
Temperature
+20to+25°C +2to+40°C −10to+60°C
Relative
Humidity
30–80%,non-
condensing
30–80%,non-
condensing
(below30°C)
10–85%,non-
condensing
(below30°C)
Altitude Approximately
500mabove
sealevel
Upto2000m
abovesealevel
Upto2000m
abovesealevel
OpentronshasvalidatedtheThermocycler’sperformanceinthe
conditionsrecommendedforsystemoperation,andoperationin
thoseconditionsshouldprovideoptimalresults.TheThermocycleris
safetouseinconditionsacceptableforsystemoperation,butresults
mayvary.Thedeviceshouldnotbepoweredonorusedinconditions
outsideofthosebounds.Thestorageandtransportationconditions
onlyapplywhenthedeviceiscompletelydisconnectedfrompower
andotherequipment.
THERMOCYCLER MODULE GEN2
Safety Information and Regulatory Compliance
Warning:SettingtheThermocyclertoholdatemperaturebelowthe
ambienttemperatureforalongtimecancausecondensationtodevelopin
andonthedevice.Do not set the plate temperature below the ambient
temperature for more than 2 hours,astheresultingcondensationcould
aectperformanceordamagethemodule.
IfyounoticecondensationontheThermocycler’splateafteruse,remove
anylabwarefromthemoduleandusetheOpentronsApptoseta
temperatureof40°Cuntilthecondensationisnolongervisible.
Software Requirements
TheThermocyclerrequiresversion6.2.0orneweroftheOpentronsApp
andOT-2robotserver.
YoucandownloadtheOpentronsAppforWindows,macOS,or
Ubuntuathttps://opentrons.com/ot-app/.
Labware Compatibility
TheThermocycleriscompatiblewithfull-skirted,96-wellPCRplates.
Opentrons’performancespecicationsarebasedonusewithOpentrons
ToughPCRPlates,whichyoucanpurchaseathttps://shop.opentrons.com.
7
Safety Information and Regulatory Compliance
SAFETY WARNING LABELS
WarninglabelspostedontheOpentronsThermocyclerandinthis
manualwarnyouaboutsourcesofpotentialinjuryorharm.Table1
providesakeytoeachsafetywarninglabel.
NOTE:Frenchsafetywarningsarelistedinthebackofthemanual.
(REMARQUE:Lesavertissementsdesécuritéenfrançaissetrouventà
landumanuel.)
CAUTION: Risk of danger! This symbol identies
instrument components that pose a risk of personal
injury or instrument damage if improperly handled.
Wherever this symbol appears, please consult the
manual for further information on safe handling
before proceeding.
CAUTION: Risk of electrical shock! This symbol
identies instrument components that pose a risk of
electrical shock if handled improperly.
CAUTION: Hot surface! This symbol identies
instrument components that, if handled improperly,
pose a risk of personal injury due to high
temperature.
CAUTION: Pinch point! This symbol identies
instrument components (namely, the automated
lid) which can pose risk of personal injury due to its
closing mechanism. Please do not reach near the lid
when it is in operation.
Table 1.Instrumentsafetywarninglabels.
THERMOCYCLER MODULE GEN2
Safety Information and Regulatory Compliance
INSTRUMENT SAFETY WARNINGS
WarninglabelspostedontheOpentronsThermocyclerreferdirectly
tothesafeuseoftheinstrument,asdescribedinTable2.
NOTE:Frenchsafetywarningsarelistedinthebackofthemanual.
(REMARQUE:Lesavertissementsdesécuritéenfrançaissetrouventà
landumanuel.)
Warning about risk of harm to body or equipment.
Operating the Opentrons Thermocycler before
reading this manual poses a risk of personal injury
or instrument damage. Only qualied laboratory
personnel should operate this instrument.
Warning about risk of harm to body or equipment
from electrical shock. Do not attempt to repair
or remove the outer case of the Opentrons
Thermocycler or its power supply unless directed by
Opentrons Support. Failure to do so puts you at risk
of electrical shock.
Warning about risk of burning. The Opentrons
Thermocycler generates enough heat to cause
serious burns. Wear safety goggles or other eye
protection at all times during operation. Always
ensure the sample block returns to idle temperature
before opening the lid and removing samples. Always
allow maximum clearance to avoid accidental burns.
Unplug the unit after use, if possible.
Warning about risk of explosion. The Opentrons
Thermocycler sample blocks can become hot enough
during the course of normal operation to cause
liquids to boil and explode.
Table 2.Instrumentsafetywarninglabels.
9
Safety Information and Regulatory Compliance
STANDARDS COMPLIANCE
TheThermocyclerhasbeentestedandfoundtobeincompliance
withallapplicablerequirementsofthefollowingsafetyand
electromagneticstandards:
Safety
◾IEC/UL/CSA61010-1SafetyRequirementsforElectricalEquipment
forMeasurement,Control,andLaboratoryUse–Part1:General
Requirements
◾IEC/UL/CSA61010-2-010ParticularRequirementsforLaboratory
EquipmentfortheHeatingofMaterials
Electromagnetic Compatibility
◾EN/BSI61326-1ElectricalEquipmentforMeasurement,Control
andLaboratoryUse–EMCRequirements–Part1:General
Requirements
◾EN55011Industrial,ScienticandMedicalEquipment–Radio-
FrequencyDisturbanceCharacteristics–LimitsandMethodsof
Measurement
◾FCC47CFRPart15SubpartBClassA:UnintentionalRadiators
◾ICICES-003SpectrumManagementandTelecommunications
–Interference-CausingEquipmentStandard–Information
TechnologyEquipment(IncludingDigitalApparatus)
THERMOCYCLER MODULE GEN2
Safety Information and Regulatory Compliance
FCC Warnings and Notes
Warning:Changesormodicationstothisunitnotexpresslyapprovedby
Opentronscouldvoidtheuser’sauthoritytooperatetheequipment.
Thisdevicecomplieswithpart15oftheFCCRules.Operationissubject
tothefollowingtwoconditions:(1)Thisdevicemaynotcauseharmful
interference,and(2)thisdevicemustacceptanyinterferencereceived,
includinginterferencethatmaycauseundesiredoperation.
Note:Thisequipmenthasbeentestedandfoundtocomplywiththe
limitsforaClassAdigitaldevice,pursuanttopart15oftheFCCRules.
Theselimitsaredesignedtoprovidereasonableprotectionagainst
harmfulinterferencewhentheequipmentisoperatedinacommercial
environment.Thisequipmentgenerates,uses,andcanradiateradio
frequencyenergyand,ifnotinstalledandusedinaccordancewith
theinstructionmanual,maycauseharmfulinterferencetoradio
communications.Operationofthisequipmentinaresidentialareaislikely
tocauseharmfulinterferenceinwhichcasetheuserwillberequiredto
correcttheinterferenceattheirownexpense.
Canada ISED Compliance
CanadaICES-003(A)/NMB-003(A)
ThisproductmeetstheapplicableInnovation,ScienceandEconomic
DevelopmentCanadatechnicalspecications.
Leprésentproduitestconformeauxspécicationstechniquesapplicables
d’Innovation,SciencesetDéveloppementéconomiqueCanada.
CISPR 11 Class A
Caution:Thisequipmentisnotintendedforuseinresidential
environmentsandmaynotprovideadequateprotectiontoradioreception
insuchenvironments.
11
Product Specifications
MODEL NUMBER
TCG2
INCLUDED PARTS
(1) Thermocycler (1) Power Supply and
Power Supply Connector
(1) Power Cable (1) USB Cable
(5) Rubber Automation Seals (10) Opentrons
Tough PCR Plates
THERMOCYCLER MODULE GEN2
Product Specifications
PHYSICAL SPECIFICATIONS
◾Dimensions:
Lidopen:244.95mmLx172mmWx310.1mmH
Lidclosed:244.95mmLx172mmWx170.35mmH
◾Weight:8.4kg(includingrearduct)
TEMPERATURE PROFILE
◾Thermalblocktemperaturerange:4–99°C
◾Thermalblockmaximumheatingramprate:4.25°C/s
betweenambientand95°C
◾Thermalblockmaximumcoolingramprate:2.0°C/s
between95°Candambient
◾Lidtemperaturerange:37–110°C
◾Lidtemperatureaccuracy:±1°C
LED STATUS LIGHT
TheThermocyclerhasastripofLEDsthatcandisplayfourcolors:
white,orange,red,andblue.Thepossiblemoduleconditions
indicatedbythelightstripare:
LED COLOR AND BEHAVIOR MODULE CONDITIONS
Solidwhite Idle
Flashingorange Error
Pulsingred Changingtohottemperature(>23°C)
Solidred Holdingathottemperature(>23°C)
Pulsingblue Changingtocooltemperature(<23°C)
Solidblue Holdingatcooltemperature(<23°C)
Warning:ThestatusLEDwilldisplaysolidwhitewheneverthe
moduleisnotactivelyheatingorcooling,regardlessofitscurrent
temperature.Itmaystillbehottothetouch.
13
Setup
VENTILATION REQUIREMENTS
ToallowforsucientfanoutputfromtheThermocyler,atleast20
cm/8inchesofemptyspaceisrequiredbetweentheunitanda
wall.
8”
20 cm
FRONT
THERMOCYCLER MODULE GEN2
Setup
Alsoforventilation,andtoprovideenoughspaceforthepowerand
USBcablestoconnecttothemodule,Opentronsadvisesusingthe
OT-2sideandrearpanelsshownbelow:
Note: These panels are included with newer models of the OT-2.
They only need to be replaced on older models of the OT-2.
ToaddnewpanelstoyourOT-2:
15
Carefully place the Opentrons Thermocycler in deck
slots 7, 8, 10, and 11. Lift the module carefully, using
two hands (the module weighs 8.4 kg, which is quite
heavy for its size).
1
Setup
SETUP STEPS
Deck Placement and Connection
THERMOCYCLER MODULE GEN2
Setup
Connect the Thermocycler to the OT-2 and power
supply by plugging in the USB cable and screwing the
power cable into the unit as shown. Be sure to route
the cables through the opening in the polycarbonate
side panel.
2
Connect the USB cable to the module and an open
USB port at the back of the robot
Connect the power supply connector to the
Thermocycler and then screw it on for safety
Connect the power cable to the back of the power
supply and the wall outlet
17
Setup
3Once everything is connected, power on the OT-2 and
the Thermocycler power supply. If you see a white light
on the Thermocycler, it is powered on. You can open
and close the lid of the Thermocycler by pressing the
round button on the front.
THERMOCYCLER MODULE GEN2
Setup
Rubber Automation Seals
YourOpentronsThermocyclercomeswith5rubberautomation
sealstohelpreduceevaporation.Eachsealmustbesterilized
beforeuseandcanbeusedforseveralruns.Youshouldcheck
thestateofthesealaftereachrunandreplaceitifneeded.
Wornordamagedsealsmayresultinincreasedevaporationof
samples.
4Apply a seal to the lid of the module, not directly to the
plate, to enable pipetting access.
5After applying the seal, wipe it with a 1:10 dilution
of bleach and let it dry. Wipe it down with molecular
biology grade water and let it dry again.
Note:SealsmustbesterilizedbeforeyourrstThermocyclerrun.
Thermocyclersealscannotbeautoclaved.
19
PLATE LATCHING
ThermocyclerGEN2doesnotincludeaplatelatch,andlatching
plateswithahardpolycarbonateframeshouldnotbenecessary.
Ifyourapplicationrequiressofterplatesandyouhaveproblems
withplatesstickingtothesealwhenopeningthelid,contact
OpentronsSupporttoobtainaplatelatchmechanism.
EMERGENCY LID OPENING
Incaseofapoweroutage,connectivityissue,orotherelectrical
failure,theThermocyclerlidmaynotopen.Ifyouneedto
manuallyopenittoretrieveyoursamples:
Setup
1Turn the unit o (if possible) and unplug it.
2Insert the included hex wrench into the circular slot on
the left side of the module, as shown.
3Push the wrench inward 25 mm / 1 inch.
4Manually lift the lid.
THERMOCYCLER MODULE GEN2
Maintenance
CLEANING
BeforecleaningtheThermocycler,unplugthepowerandUSBcables.
DonotcleanthetemperatureindicatorLEDstrip,powerbutton,
powerinputport,orUSBportoftheThermocycler.
Allothersurfacesarecompatiblewith:
◾10%bleachsolution
◾70%ethanol
◾99%isopropylalcohol
Sprayoneofthesecleanersontoapapertowelanduseittowipethe
followingpartsoftheThermocycler:
◾PCRtopplate
◾Lidheatingplate
◾Modulehousing/body
◾Lidplastichousing
◾Sheetmetalhousing
◾Rubberseal
Whendone,wipedownthesepartswithadrypapertowel.
AvoidintroducingcleaningsubstancesintotheUSBportorpower
port.Neverdouseorspraythemoduledirectlywithcleaner.
Repair
UsersshouldnotattempttoserviceorrepairtheThermocycler
Modulethemselves.Ifyouhaveconcernsaboutyourmodule’s
performanceorrequirerepairs,pleasecontactOpentronsSupport.
Warranty
AllhardwarepurchasedfromOpentronsiscoveredundera1-year
standardwarranty.Opentronswarrantstotheend-userofthe
productsthattheywillbefreeofmanufacturingdefectsdueto
partqualityissuesorpoorworkmanshipandalsowarrantsthat
theproductswillmateriallyconformtoOpentrons’published
specications.
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