Opentrons Flex User manual

1-Channel Pipette
50 µL, 1000 µL
The Opentrons Flex 1-Channel Pipette is an electronic
pipette designed for the Opentrons Flex liquid handling
robot. This guide describes the pipette’s features,
including basic installation, maintenance, and warranty
information.
Pipette Capacity
Flex 1-channel pipettes are available in 50 µL and 1000 µL
sizes. The minimum/maximum volumetric capacities of
each type are listed below.
50 µL pipette: 1–50 µL
1000 µL pipette: 5–1000 µL
Box Contents
Tip Compatibility
Flex pipettes are optimized for use with Flex 50 µL,
200 µL, and 1000 µL pipette tips. Flex pipettes are not
recommended for use with Opentrons OT-2 pipette tips or
tips from other manufacturers. For more information, see
the System Description and Labware chapters in the Flex
instruction manual.
You can buy Flex tips here: https://shop.opentrons.com.
(1) Pipette
(4) Replacement O-rings
(2) O-ring tools
(1 remover, 1 installer)

Warranty
All hardware purchased from Opentrons is covered
under a 1-year standard warranty. Opentrons warrants
to the end-user of the products that they will be free
of manufacturing defects due to part quality issues or
poor workmanship and also warrants that the products
will materially conform to Opentrons’ published
specications.
Support
Opentrons Support can help you with questions about
our products and services. If you discover a defect, or
believe your product is not functioning to published
specications, contact us at [e-mail].
Please have the pipette’s serial number available when
contacting support. You can nd the serial number
on the back of the pipette or via the touchscreen (tap
Instruments and tap the pipette name).
© OPENTRONS 2023
Opentrons Flex™(Opentrons Labworks, Inc.)
Registered names, trademarks, etc. used in this document, even when not
specically marked as such, are not to be considered unprotected by law.
Installation and Maintenance
Information about pipette installation and care.
ATTACH
Instructions on the touchscreen, or in the Opentrons
App, will guide you through the attachment process.
On the touchscreen, tap Instruments and then tap
an empty mount. To attach your pipette:
1. Clear the deck area so it is free from
obstructions. The gantry will move during the
installation process.
2. Connect the pipette to the left or right mount
on the gantry. To attach, align the mounting
holes on the back of the pipette with the pins
on the gantry.
3. Secure the pipette by tightening the two 2.5
mm mounting screws. These are captive screws
recessed into holes located on the front of the
pipette.
CALIBRATE
The pipette requires calibration before use.
Instructions on the touchscreen, or in the Opentrons
App, will guide you through the calibration process.
To calibrate your pipette:
1. Clear the deck area so it is free from
obstructions. The gantry and pipette will move
during calibration.
2. Attach the calibration probe to the pipette and
start the automated calibration procedure.
3. Remove and store the probe when calibration is
complete. Your pipette is ready for use.
CLEAN
Clean the outside of the pipette with a soft cloth and
isopropyl alcohol or ethyl alcohol. Do not use acetone
or other aggressive solvents. For information about
cleaning and decontamination, scan the QR code or
search for “Flex pipette cleaning” on
support.opentrons.com.