Opentrons Thermocycler Module GEN2 User manual

Thermocycler Module GEN2
Quickstart Guide
Opentrons Labworks Inc.
Edition
GEN2, December 2022

– Maintenance
– Support
– App Download
Table of Contents
Post-sales service & contacting Opentrons
If you have any questions about the use of the module
or your Opentrons platform, please contact:
[email protected]. Also visit www.opentrons.com.
Product Elements 3
Setup Instructions 4
Resources 10
Product and Manufacturer Description 11
– Product Description
– Manufacturer Description
– Ventilation Requirements
– Deck Placement and Connection
– Rubber Automation Seals

3
Product Elements
(1) Thermocycler
(1) Power Cable (1) USB Cable
(1) Power Supply and
Power Supply Connector
(5) Rubber Automation Seals (10) Opentrons
Tough PCR Plates

THERMOCYCLER MODULE GEN2
Setup Instructions
VENTILATION REQUIREMENTS
To allow for sucient fan output from the Thermocyler,
at least 20 cm / 8 inches of empty space is required
between the unit and a wall.
8”
20 cm
FRONT

5
Also for ventilation, and to provide enough space for the
power and USB cables to connect to the module, Opentrons
advises using the OT-2 side and rear panels shown below:
2x Side Panels 1x Rear Panel
Note: These panels are included with newer models of the
OT-2. They only need to be replaced on older models of
the OT-2.
Setup Instructions

THERMOCYCLER MODULE GEN2
Setup Instructions
DECK PLACEMENT AND CONNECTION
Carefully place the Opentrons Thermocycler in
deck slots 7, 8, 10, and 11. Lift the module care-
fully, using two hands (the module weighs 8.4 kg,
which is quite heavy for its size).
1
TRASH
PLACEMENT OF
THERMOCYCLER

7
Setup Instructions
Connect the Thermocycler to the OT-2 and power
supply by plugging in the USB cable and screwing
the power cable into the unit as shown. Be sure
to route the cables through the opening in the
polycarbonate side panel.
2
Connect the USB cable to the module and an open
USB port at the back of the robot
Connect the power supply connector to the
Thermocycler and then screw it on for safety
Connect the power cable to the back of the power
supply and the wall outlet

THERMOCYCLER MODULE GEN2
Setup Instructions
Once everything is connected, power on the OT-2
and the Thermocycler power supply. If you see a
white light on the Thermocycler, it is powered on.
You can open and close the lid of the Thermocycler
by pressing the round button on the front.
3

9
Setup Instructions
RUBBER AUTOMATION SEALS
Your Opentrons Thermocycler comes with 5 rubber
automation seals to help reduce evaporation. Each seal
must be sterilized before use and can be used for several
runs. You should check the state of the seal after each
run and replace it if needed. Worn or damaged seals may
result in increased evaporation of samples.
Apply a seal to the lid of the module, not directly
to the plate, to enable pipetting access.
4
After applying the seal, wipe it with a 1:10
dilution of bleach and let it dry. Wipe it down with
molecular biology grade water and let it dry again.
Note: Seals must be sterilized before your rst
Thermocycler run. Thermocycler seals cannot be
autoclaved.
5

THERMOCYCLER MODULE GEN2
Resources
MAINTENANCE
Users should not attempt to service or repair the
Thermocycler Module themselves. If you have
concerns about your module’s performance or require
maintenance, please contact Opentrons Support.
SUPPORT
Opentrons Help Center
Answers to many common questions are available on the
Opentrons Help Center at https://opentrons.com/support.
Contact Opentrons
If you have any other questions, contact
[email protected]. Please have the serial numbers
of your module and robot available when contacting
Opentrons Support.
Warranty
All hardware purchased from Opentrons is covered
under a 1-year standard warranty. Opentrons warrants
to the end-user of the products that they will be free of
manufacturing defects due to part quality issues or poor
workmanship and also warrants that the products will
materially conform to Opentrons’ published specications.
APP DOWNLOAD
Control your liquid handling robot and module using the
Opentrons App. Download the app for Windows, macOS,
or Ubuntu at https://opentrons.com/ot-app/.

11
PRODUCT DESCRIPTION
The Opentrons Thermocycler Module GEN2 provides on-
deck, fully automated thermocycling. The module enables
automation of upstream and downstream workow steps.
Completed Certications
IEC/UL/CSA, EN/BSI, FCC, IC
Recommended Operating Conditions
Environmental temperature: 20–25 °C
Environmental humidity: 30–80%, non-condensing
MANUFACTURER DESCRIPTION
Opentrons Labworks Inc
45-18 Ct Square W
Long Island City, NY 11101
Product and Manufacturer Description

For a PDF of the complete Opentrons Thermocycler
Module GEN2 Instruction Manual, scan this QR code: