Opentrons OT-2 User manual

OT-2 Liquid Handling Robot
User Manual / Quickstart Guide
Opentrons Labworks Inc.
Edition
Revision OT-2R, June 2021

Product and Manufacturer Description
Product Elements/Overview
Instructions for Unboxing and Use
– Included Box Contents
– Required for Operation
– Machine Setup
– Remove the Transport Fasteners
– Check the Machine
– Pipette Installation
– Pipette Removal
– Power and Connection
– Software Interface
Online Resources
Cleaning and Maintenance
Support
Table of Contents
Post sales service & contacting Opentrons
If you have any questions about the use of the system, abnormal
phenomena, special needs, please contact:
[email protected]. Also visit www.opentrons.com.
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OT-2 MANUAL
Product and Manufacturer
Description
Product Elements
PRODUCT DESCRIPTION
The OT-2 is a liquid handling lab robot. It is open-source, highly
customizable, kit and reagent agnostic, and takes up half a lab bench.
Its software and hardware modules are built by scientists for scientists
and don’t require coding knowledge to use.
Completed Certications
CE & FCC, NRTL, & CB
Not Certied/Validated
IVD, GMP & ISO
MANUFACTURER DESCRIPTION
Opentrons Labworks Inc
20 Jay Street, #528
Brooklyn, New York
NY 11201
Serial Number
OT2XXX2021XXXXRXX
OT-2
66CM/26IN
57CM/22.5IN 63CM/25IN
0.1mm resolution
xyz gantry
Polycarbonate
enclosure
Easily removable deck
2 interchangeable
pipette heads

OT-2 MANUAL
Instructions for
Unboxing and Use
Product Elements
DECK OUTLINE
11 deck slots
Adapters allow for existing
hardware to t on deck
Deck slots are compatible with standard SBS
dimensions. Deck also includes a removable trash bin.
Modules snap into place
To get started quickly, visit our online resources and follow the
step by step instructions to help you go from unboxing your
robot to running your rst protocol.
Visit https://support.opentrons.com/ for more information.
INCLUDED BOX CONTENTS / PARTS & ACCESSORIES
◾(1) OT-2
◾(3) Window Covers
◾(2) Side Window Covers
◾(1) Rear Window Cover
◾(1) Power supply (36V/6A)
◾(4) IEC Power cable (Connects the power supply bar to a wall
socket)
◾IEC Cable US
◾IEC Cable UK
◾IEC Cable EU
◾IEC Cable Italy
◾(1) Ethernet Cable
◾(1) Ethernet-to-USB dongle (connects the OT-2 to a laptop)
◾(1) Tool Kit
◾Hand Screwdriver (2.5mm)
◾14mm Wrench
◾Torx Key (T10)
◾Allen key set (1.5mm, 2mm, 2.5mm, 3mm)
◾Super Lube (silicone based lubricant)
◾2pcs M3 hex nut
◾2pcs M4 square nut

OT-2 MANUAL
Box C Included Box Contents
/ Parts & Accessories
◾Pipette Screws
◾4 pieces of M3x6mm socket head screws (2 are spares)
◾Window Screws
◾4x top window screws (M4x12mm low prole socket
screw, M4 thick washer, and rubber O-ring)
◾(1) Trash Bin
◾(1) Calibration Block
(1) OT-2
(4) Window Covers
(2 side window covers, 1
rear window cover)
(1) Power supply (36V/6A)
(4) IEC Power Cable
(Connects power supply bar
to a wall socket)
- IEC Cable US
- IEC Cable UK
- IEC Cable EU
- IEC Cable Italy
ITALY USA
UK EU
(1) Ethernet Cable
(1) Ethernet-to-USB dongle
(Connects the OT-2 to a
laptop)
(1) Torx Key (T10)
(1) Super Lube
(silicone based lubricant)
(4) Pipette Screws
(4 pieces of M3x6mm socket
head screws, 2 are spares)
(1) 14mm Wrench
(1) Allen Key Set
(1.5mm, 2mm,
2.5mm, 3mm)
(1) Hand Screwdriver
(2.5mm)
(2) 2pcs M3 hex nut
(2) 2pcs M4 square nut

OT-2 MANUAL
REQUIRED FOR OPERATION:
◾A computer (self-provided), an open-end wrench (included)
◾Space required by the machine: Space larger than
625mm*570mm*680mm
(4) Window Screws
(4x top window screws (M4x12mm
low prole socket screw, M4 thick
washer, and rubber O-ring)
(1) Calibration Block
(1) Trash Bin
Instructions
MACHINE SETUP
Remove the top foam and window and top covers.
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Carefully cut open the plastic VCI bag. Be sure to avoid
cutting any machine components inside.
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OT-2 MANUAL
The machine requires two people for lifting out of the
packaging (the machine is over 30kg).
Pull upwards using the black steel frame.
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Cut the zip ties supporting the customer kit and place aside.
Place the machine on a secure and stable surface.
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Instructions

OT-2 MANUAL
Pull the remaining zip tie ends and remove completely from
the deck.
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Remove tape from the calibration switch cover.
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Remove the plastic cover completely.
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Place the trash bin in place.
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Instructions

OT-2 MANUAL
Remove the 3 transport fasteners:
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1. Unlock and remove the X-axis locking bracket (red) and 4x
locking screws using the 3mm allen key
2. Unlock and remove the Y-axis locking bracket (red) and 1x
locking screw using the 3mm allen key
3. Unlock and remove the Z-axis locking bracket (red) and 4x
locking screws using the 3mm Allen key. Note that after
removing the rear screws, one can move the z-stage further
left by hand, allowing more room for removing the screw.
Instructions Remove the lm used to protect the polycarbonate sheets
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Install the top polycarbonate sheet and fasten the four
corner screws using the 2.5mm hex driver and 4x top
window screws
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OT-2 MANUAL
Check the Machine:
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Conrm that 3 brackets have been removed, as powering
on the robot with brackets may cause damage.
Conrm that you are not missing any required cables and/
or parts. If you are missing components, contact Customer
Support.
Visually check the deck and windows, if there are any
signicantly scratched, broken, or bent components,
contact Customer Support.
Visually check the gantry, if it is uneven or slanted, contact
Customer Support.
Move the pipette head in the x and y direction, if it makes
any loud/abnormal noises or requires a large thrust to
move, contact Customer Support.
Clean & Sterilize Robot
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Note: When cleaning and maintaining the machine, be sure
to turn o the machine and disconnect the power before
proceeding. Also pay attention to warning labels
Instructions Install window covers
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OT-2 MANUAL
Connect Power Source + USB/Ethernet Cable
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POWER AND CONNECTION
Connect ethernet and connect the power cable.
If your computer does not have ethernet use the USB to
ethernet dongle provided.
POWER REQUIREMENTS
100-240 VAC / 50-60 Hz
220 W MAX
CONNECTIVITY
WiFi 2.4 GHz IEEE 802.11b/g/n, USB 2.0.
Instructions
Install the Opentrons App
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MIN REQUIREMENTS:
Windows 10, macOS 10.10 or later, Ubuntu 12.04 or
later.
Congratulations! Now your OT-2 robot has been successfully
unlocked and set up!
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When everything is ready, power on the machine
by pressing the power button and wait for about 45
seconds before the machine starts running and the X, Y,
and Z axes each return to the origin.
Once the robot is turned on, follow instructions in the
Opentrons App to connect to the OT-2, attach pipettes,
and calibrate the robot.

OT-2 MANUAL
Online Resources
Now that you have your robot connected, it’s time to start using it! This
section will breakdown all of our online resources so you can gure out
where to get the info you need, how to download protocols, and check
out new features!
www.opentrons.com
For consumable materials visit opentrons.com where you nd
tips, labware, and reagents.
Protocol Library - https://protocols.opentrons.com/
The Protocol Library is home to our shared protocols. The “Browse” tab
will take you to all of the protocol json les that you can download and
run on the robot, (along with handy diagrams of how to set up and
Cleaning and Maintenance
CLEANING
The OT-2 can be cleaned and disinfected with alcohol, bleach, and any
standard household cleaner using a rag or paper towel. Avoid contact
with any electronics.
The OT-2 is not a sterile environment. Add your own UV lights, or the
Opentrons HEPA Module
Note: When cleaning and maintaining the machine, be sure to turn o the
machine and disconnect the power before proceeding. Also pay attention to
warning labels.
Maintenance
The OT-2 has been tested for years of normal use without
maintenance. If there are any concerns about the OT-2 or maintenance
please contact Opentrons support.
Support
In case of any issues or questions, contact: [email protected]
Please note your OT-2’s serial number is located on the bottom right
side of the machine.
WARRANTY
One year warranty against manufacturer defects. Stress tested to last a
minimum of 3 years of full-time use.
Specs
PIPETTE CONFIGURATIONS
Single and multi-channel pipetting. 2-pipette mounts, for a
conguration of 1 or 2 single or 8-channel pipettes. Pipettes are easily
interchangeable.
PIPETTE VOLUMES AND SPECS
Single channel: 1-1000 µl
8-channel: 1-300 µl
Whitepaper: OT-2 GEN2 Single Pipettes
Whitepaper: OT-2 GEN1 Multichannel Pipettes
FRAME COMPOSITION
Rigid steel and CNC aluminum design
OPERATING TEMPERATURE & RELATIVE HUMIDITY
Recommended 20-24C. Recommended 40-60% RH. Learn more.

OT-2 MANUAL
Specs
PLATE TYPE COMPATIBILITY
Use 96 or 384 well plates. Use 96-well plates for optimal deck
compatibility & touch tips. Compatible with up to 384-well plates.
Use common labware already in our Labware Library, or add your own.
If your labware ts on the OT-2 but is not in the Labware Library, it can
be added by you using our Labware Creator, or by our team. Learn
more.
API COMPATIBILITY
Interfaces with any product that has an API. Opentrons software is
open-source and users can make adjustments to the software as
needed to interface with other products. While all integrations are
possible, they all require custom code work i.e. most customers will
need to write custom libraries and install software packages that aren’t
shipped with the robot. Learn more.