Opentrons GEN1 User manual

Opentrons GEN1
Thermocycler

3
Product and Manufacturer Description
Safety Information and Regulatory Compliance
Product Description
Setup and Maintenance
Emergency Open Lid
4
5
14
16
27
Table of Contents
Copyright ©2020 Opentrons Labworks, Inc. Reproduction in any form, either print or
electronic, is prohibited without written permission of Opentrons Labworks, Inc.

THERMOCYCLER MANUAL
5
Product and Manufacturer Description Safety Information and Regulatory Compliance
The Opentrons GEN1 Thermocycler heats and cools very quickly during
operation. We strongly recommend that you follow the safety specications
listed in this section and throughout this manual.
SAFE USE SPECIFICATIONS
Please refer to these specications and compliance guidelines to ensure
safe usage of The Opentrons Thermocycler. Figure 1 illustrates safe use
specications for all input and output connections for the module and its
power supply, including electrical input and PC connection, including the
sticker bearing this information on the module:
◾Do NOT replace cord unless at the direction of Opentrons customer
support
◾AC IEC power connection to power supply
◾DC power connection to thermocycler: 36 Volts DC
◾USB (with USB Symbol)
◾The following copy is shown on the instrument sticker:
– Name: Thermocyler
– Model: GEN1
– DC Input: 36V
– Max: Power: 36 VDC 17.50
◾Rated conditions for all input/output connections (e.g. voltage, current)
PRODUCT DESCRIPTION
The Opentrons GEN1 Thermocycler module is an easy-to-use on-deck
module that enables automation of upstream and downstream workow
steps. It is a fully automated thermocycler. This guide will help you learn how
to get the best use of it.
MANUFACTURER DESCRIPTION
Opentrons Labworks Inc
20 Jay Street, #528
Brooklyn, New York
NY 11201

THERMOCYCLER MANUAL
7
SAFETY WARNING LABELS
Warning labels posted on the Opentrons Thermocycler and in this manual
warn you about sources of potential injury or harm. A key for each safety
warning label is referenced in Table 1.
NOTE: French safety warnings are listed in the back of the manual.
(REMARQUE: Les avertissements de sécurité en français se trouvent à la n
du manuel.)
POWER SUPPLY (AC)
a. Max Power (W) 630 Watts
b. Max Power (VA)
100-240VAC
C. 8.5
THERMOCYCLER (DC)
a. Max Power (W) 630 Watts
b. Max Power (VA) 36 VDC
c. Max Current 17.5A
ICON MEANING
CAUTION: Risk of danger! This symbol identies
instrument components that pose a risk of personal
injury or instrument damage if improperly handled.
Wherever this symbol appears, please consult the
manual for further information on safe handling
before proceeding.
CAUTION: Risk of electrical shock! This symbol
identies instrument components that pose a risk of
electrical shock if handled improperly.
CAUTION: Hot surface! This symbol identies instru-
ment components that pose a risk of personal injury
due to excessively high heat temperature if handled
improperly.
CAUTION: Pinch Point! This symbol identies instru-
ment components (namely, the automated lid) which
can pose risk of personal injury due to its closing
mechanism. Please do not reach near the lid when it is
in operation.
Table 1. Instrument safety warning labels

THERMOCYCLER MANUAL
9
ICON MEANING
Warning about risk of harm to body or equipment
from electrical shock.
Do not attempt to repair or remove the outer case of
the Opentrons Thermocycler or its power supply unless
directed by Opentrons’ support team. Failure to do so
puts you at risk of electrical shock.
Warning about risk of harm to body or equipment.
The Opentrons Thermocycler generates enough heat
to cause serious burns. Wear safety goggles or other
eye protection at all times during operation. Always
ensure the sample block returns to idle temperature
before opening the lid and removing samples. Always
allow maximum clearance to avoid accidental burns.
Unplug the unit after use, if possible.
Warning about risk of explosion.
The Opentrons Thermocycler sample blocks can
become hot enough during the course of normal
operation to cause liquids to boil and explode.
Table 2 Continued: Instrument safety warning labels
INSTRUMENT SAFETY WARNINGS
Warning labels posted on the Opentrons Thermocycler refer directly to the
safe use of the instrument, as explained in Table 2.
NOTE: French safety warnings are listed in the back of the manual.
(REMARQUE: Les avertissements de sécurité en français se trouvent à la n
du manuel.)
Table 2. Instrument safety warning labels
ICON MEANING
Warning about risk of harm to body or equipment.
Operating the Opentrons Thermocycler before
reading this manual poses a risk of personal injury
or instrument damage. Only qualied laboratory
personnel should operate this instrument.

THERMOCYCLER MANUAL
11
SAFETY AND REGULATORY COMPLIANCE
This instrument has been tested and found to be in compliance with
all applicable requirements of the following safety and electromagnetic
standards (explained in Table 3).
Table 3. Safe use specications
SAFETY COMPLIANCE
This instrument has been tested and found to be in compliance with
all applicable requirements of the following safety and electromagnetic
standards:
◾IEC 61010-1:2001 Safety requirements for electrical equipment
for measurement, control, and laboratory use -Part 1: General
requirements
◾IEC 61010-2-010:2003 Safety requirements for electrical equipment
for measurement, control, and laboratory use -Part 2-010: Particular
requirements for laboratory equipment for the heating of material
◾CAN/CSA-C22.2 NO. 61010-1-04 Safety Requirements for Electrical
Equipment for Measurement, Control, and Laboratory Use - Part 1:
General Requirements
◾CAN/CSA-C22.2 NO. 61010-2-010-04 Safety Requirements for Electrical
Equipment for Measurement, Control, and Laboratory Use - Part 2-010:
Particular Requirements for Laboratory Equipment for the Heating of
Materials
◾EN 61010-1:2001 Safety requirements for electrical equipment
for measurement, control, and laboratory use -Part 1: General
requirements
◾EN 61010-2-010:2003 Safety requirements for electrical equipment
for measurement, control, and laboratory use -Part 2-010: Particular
requirements for laboratory equipment for the heating of material
◾UL 61010-1:2004(R2008) Safety Requirements for Electrical Equipment
for Measurement, Control, and Laboratory Use -Part 1: General
Requirements
USAGE ASPECT CONDITIONS FOR SAFE USER
Rated input power (VA) 100-240VAC 8.5-5A
36 VDC 17.5A
Over-voltage category Category II
Environment Indoor use only
Ambient Temperature 20-24°C
Relative humidity Up to 80%
Altitude Up to 2,000 meters above sea
level

THERMOCYCLER MANUAL
13
◾UL 61010A-2-010:2002 Standard for Electrical Equipment for Laboratory
Use; Part 2: Particular Requirements for Laboratory Equipment for the
Heating of Material
ELECTROMAGNETIC COMPATIBILITY (EMC)
◾IEC61326-1:2005 Electrical Equipment for measurement, control, and
laboratory use -EMC Requirements, Class A
◾EN61326-1:2006 Electrical Equipment for measurement, control, and
laboratory use -EMC Requirements, Class A
◾FCC Part 15, Subpart B, Sections 15.107 and 15.109 as a Class A digital
device.
FCC WARNINGS AND NOTES
WARNING! Changes or modications to this unit not expressly approved
by Opentrons Labworks Inc, could void the user’s authority to operate the
equipment.
◾Note: This equipment has been tested and found to comply with the
limits for a Class A digital device, pursuant to part 15 of the FCC Rules.
These limits are designed to provide reasonable protection against
harmful interference when the equipment is operated in a commercial
environment. This equipment generates, uses, and can radiate radio
frequency energy and, if not installed and used in accordance with
the instruction manual, may cause harmful interference to radio
communications. Operation of this equipment in a residential area
is likely to cause harmful interference in which case the user will be
required to correct the interference at his own expense.
◾Note regarding FCC compliance: Although this instrument has been
tested and found to comply with Part 15, Subpart B of the FCC Rules for
a Class A digital device, please note that this compliance is voluntary,
for the instrument qualies as an “exempted device” under 47 CFR
15.103(c), in regard to the cited FCC regulations in eect at the time of
manufacture.
◾Note regarding Canadian EMC compliance: Le present appareil
numerique n’emet pas de bruits radioelectrique depassant les limites
applicables aux appareils numeriques de class A prescrites dans le
reglement sur le brouillage radioelectrique edicte par le Ministere des
Communications du Canada.
CISPR 11 CLASS A
◾WARNING: Class A equipment is intended for use in an industrial
environment. In the documentation for the user, a statement shall be
included drawing to the fact that there may be potential diculties in
ensuring electromagnetic compatibility in other environments, due to
conducted as well as radiated disturbances.

THERMOCYCLER MANUAL
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Product Description
Model Number: GEN1
Diagram of included parts:
1x Thermocycler
1x Power cable
(Connects the power supply
bar to a wall socket)
1x USB 2.0 cable
(Connects the Thermocyler
to the OT-2)
5x Automation seals
(Please sterilize before use)
1x Power supply and power
supply connector
(Connects the Thermocycler
to the power supply bar)

THERMOCYCLER MANUAL
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Setup and Maintenance
VENTILATION REQUIREMENTS (E.G. FREE SPACE FOR FAN OUTPUT)
◾20 cm / 8 inches between the unit and a wall
SETUP STEPS
Setup Thermocycler Compatible Panels
Powering Up and Deck Placement
Silicone Automation Seals
Sterilizing Seals
Latching Plates
1
2
4
3
5
8”
20 cm
Front

THERMOCYCLER MANUAL
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1Thermocycler Compatible Panels
To help enable adequate ventilation for the Opentrons Thermocycler, and to
provide enough space for the power/USB cords to connect to the module -
we advise that your OT-2 robot includes the ventilation panels shown below.
NOTE: this step is only required for older models of the OT-2.
If your robot already has the cutout in the bottom left of the back
panel, skip ahead to Step 2.
2x Side Panels
OT-2 Panel Diagram
1x Rear Panel
To add new panels to your OT-2:
◾Take o the existing left, rear and right acrylic panels with a 2.5mm
hex screwdriver
One was included with your OT-2, but any 2.5mm hex
screwdriver will do
◾Screw in the new polycarbonate panels
We will check if your model needs the new panels when you order a
thermocycler.
To clean the new panels, use 70% ethanol.

THERMOCYCLER MANUAL
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Powering Up and Deck Placement
1. Carefully place the Opentrons Thermocycler in deck slots 7, 8, 10
and 11. Please lift carefully, using two hands (the unit is 7.6 kg,
which is quite heavy for its size).
Connect USB 2.0 Cable to Raspberry Pi (like other modules)
Power Supply Connector plugs into Thermocycler then
then needs to be screwed on for safety
Power Cable plugs into back of Power Supply and to the
wall outlet
2. Connect the Opentrons Thermocycler to the power supply and OT-2
by plugging in the USB cord and screwing the power cord into the
unit as shown in the diagram. Be sure to weave the cords through
the opening in the polycarbonate panel.
2

THERMOCYCLER MANUAL
3. Once everything is connected, turn on the OT-2 and the Opentrons
Thermocycler power supply. If you see a white light on the
Opentrons Thermocycler, it is powered on. You may open and close
the lid of the Opentrons Thermocycler by pressing the round button
on the front.
Rear Side of
Power Supply
Left side of thermocycler
Operational Requirements
The Opentrons Thermocycler requires the latest version of the Opentrons
App and OT-2 operating system which can be found here:
› opentrons.com/ot-app
Connectivity and Usage instructions
Full connectivity, usage, and labware compatibility instructions for using the
Opentrons Thermocycler with the OT-2 can be found here:
› opentrons.com/support/thermocycler
23

Sterilizing Seals
NOTE: Seals must be sterilized before your rst thermocycler run.
Apply the seal to the lid. Wipe it with a 1:10 dilution of bleach. Let it dry. Wipe
it down with molecular biology grade water. Let it dry.
4
25
Latching Plates
To accommodate a tight seal when opening and closing the instrument lid,
you must latch it. Please follow this diagram.
Step 1
Step 2
5
Silicone Automation Seals
Your Opentrons Thermocycler arrives with 5 silicone automation seals. Simi-
lar to a traditional seal, these silicone seals help reduce evaporation. Apply a
seal to the lid of the instrument, NOT directly to the plate, to enable pipetting
access. Each seal can be used for approximately 20 thermocycler runs.
NOTE: You MUST sterilize seals before use in order to ensure sterility.
3
THERMOCYCLER MANUAL

THERMOCYCLER MANUAL
LABWARE COMPATIBILITY
The Opentrons Thermocycler is compatible with full-skirted 96 well PCR
plates. We recommend you use the NEST 0.1 mL 96-Well PCR Plate, Full Skirt,
which you can purchase at shop.opentrons.com.
Cleaning & decontamination
For instructions on cleaning and care, go to:
› opentrons.com/support/cleaning
WARRANTY
While we try our best to ensure you get a quality product, we understand
that things can go wrong from time to time. If that happens, we will make it
right. For more information visit opentrons.com/support.
27
In case of a power outage, connectivity issue, or other electrical failure, the
thermocycler lid may not open. If you need to manually open it to retrieve
your samples, please: turn the unit o, unplug it, insert the hex wrench into
the circular slot next to the cable as shown in the diagram below, push the
wrench in an inch / 25 mm, and lift the lid.
Hex Wrench
Insertion Diagram
Emergency Open Lid

29
ÉTIQUETTES D’AVERTISSEMENT DE SÉCURITÉ
Les étiquettes d’avertissement achées sur le Thermocycler Opentrons et
dans ce manuel vous avertissent des sources potentielles de blessures ou de
dommages. Une clé pour chaque étiquette d’avertissement de sécurité est
référencée dans le Tableau 1.
Tableau 1. Étiquettes d’avertissement de sécurité des instruments.
ICÔNE SENS
ATTENTION: Risque de danger! Ce symbole identie
les composants de l’instrument qui présentent un
risque de blessure ou de dommage pour l’instrument
s’ils ne sont pas manipulés correctement. Où
que ce symbole apparaisse, veuillez consulter le
manuel pour plus d’informations sur la sécurité du
traitement avant de continuer.
ATTENTION: Risque d’électrocution! Ce symbole
identie les composants de l’instrument présentant
un risque de choc électrique en cas de manipulation
incorrecte.
ATTENTION: Surface chaude! Ce symbole identie
les composants de l’instrument présentant un risque
de blessure corporelle en raison d’une température
de chauage excessivement élevée s’ils sont manip-
ulés de manière incorrecte.
AVERTISSEMENTS DE SÉCURITÉ DES INSTRUMENTS
Les étiquettes d’avertissement apposées sur le Thermocycler Opentrons font
directement référence à l’utilisation en toute sécurité de l’instrument, comme
expliqué dans le Tableau 2.
Tableau 2. Étiquettes d’avertissement de sécurité des instruments.
ICÔNE SENS
ATTENTION: Pincer! Ce symbole identie les com-
posants de l’instrument (à savoir le couvercle autom-
atisé) qui peuvent présenter un risque de blessure
en raison de son mécanisme de fermeture. S’il vous
plaît ne pas atteindre près du couvercle lorsqu’il est
en cours d’utilisation.
ICÔNE SENS
Avertissement sur les risques de lésions corporelles
ou corporelles.
Utiliser le thermocycleur Opentrons avant de lire ce
manuel présente un risque de blessure ou de dom-
mage aux instruments. Seul un personnel de labora-
toire qualié doit utiliser cet instrument.

31
ICÔNE SENS
Avertissement concernant le risque de blessure
corporelle ou physique par électrocution.
Ne tentez pas de réparer ou de retirer le boîtier
extérieur du thermocycleur Opentrons ni son alimen-
tation, sauf indication contraire de l’équipe de support
technique d’Opentrons. Ne pas le faire vous expose à
un risque de choc électrique.
Avertissement sur le risque de brûlure.
Le thermocycleur Opentrons génère susamment
de chaleur pour causer de graves brûlures. Porter
des lunettes de protection ou une autre protection
oculaire en tout temps pendant le fonctionnement. As-
surez-vous toujours que le bloc d’échantillons retourne
à la température d’inactivité avant d’ouvrir le couvercle
et de retirer les échantillons. Toujours autoriser un dé-
gagement maximal pour éviter les brûlures accidentel-
les. Débranchez l’appareil après utilisation, si possible.
Avertissement sur le risque d’explosion.
Les blocs d’échantillons Opentrons Thermocycler
peuvent devenir susamment chauds au cours de leur
fonctionnement normal pour provoquer l’ébullition et
l’explosion de liquides.
Tableau 2 a continué. Étiquettes d’avertissement de sécurité des
instruments.