OPTO-EDU A11.6603 User manual

A11.66 03
Biological Microscope
Instruction Manual

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CONTENTS
Attentions …………………………………………………2
1. Application ……………………………………………3
2. Specifications …………………………………………4
3. Installation and use ……………………………………4
4. Maintenance ……………………………………… 10
5. Troubleshooting ………………………………………13

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ATTENTIONS
1. Disassembly only by the professionals
The microscope has been adjusted before shipping, Unprofessional-person
should not disassemble and remove any other parts. Disassemble and remove
any other parts will result in equipment damage .
If you have any questions, please contact with manufacturer or local
distributor.
2. Note the input voltage if correspond
This instrument designed for wide input voltage (100V~240V,50/60HZ),
applicable to most area. But if the supply voltage exceeds this range, the
instrument will be seriously damaged.
3. Prevent burns and fire
When using power equipment, bulbs and collecting mirror and other nearby
parts of the set will rise sharply in temperature until it reaches a thermal
equilibrium state. Pay attention to anti-hot logo, they should be careful not be
burn when in use.
Alcohol, gasoline, paper and other flammable materials can’t near the
lamp in case of fire.
4. Notes on replacing the bulb
Replacement should be based on the identity of the instrument using the same
specifications of the bulb (3.4V/3W LED or G4 12V/20W halogen), otherwise
it may cause equipment damage.
The power supply must be cut off before bulb replacement, the bulb must be
cooled off completely before proceeding! Don’t left fingerprints,dust or oil on
the bulb.
5. Carry and operation
Power must be cut off before moving. Be careful not to crush your finger
when placed.
This instrument is a precision instrument, and it should be handle with care, severe
shock can cause serious damage to equipment-related parts.
The required available environment for using of the equipment:
Indoor temperature: 0 ℃ ~ 40 ℃ Maximum relative humidity: 85%
High temperature or high humidity may cause mildew, fog or dew of the
optical components, and make the instrument not work.
6. This eletrical instrument don’t cause electromagnetic interferernce for other
device.

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Fig.1 B Series Microscope Outside Drawing
1. Application
B series biological microscope is designed for teaching, clinical verify.
The series microscope with original style, steady structure, convenient
operation and clear image is suitable for observing various biological
specimens, and mostly applied in school, college, hospital, etc.
Binocular head
Nosepiece
Objective
Clamps
Coarse & fine
focusing
unit
Power supply
base
Main body
Eyepiece
Monocular head
Condenser
Stage
Aperture diaphragm
lever
Stage up-down
knob
Collector
Power switch

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2. Specifications
Item B series
Optical system Finity
Mechanical
tube length 160mm
Total
Magnification 40X～1600X
Objective
Category Achromatic, plan achromatic, semi-plan
Magnifying
power Based on the standard GB/T 2609.
Eyepiece
Magnification 10X. 16X（Optional）
Obervation
method Binocular. Monocular (Optional）
Observation
tube
Binocular . Monocular（Optional）,
Trinocular（Optional）
Eyepiece and
tube size φ23.2 8
8
h
F
Nosepiece Quadruple
Condenser Abbe
Stage Mechanical stage,Fixed stage（Optional）
Focusing Unit Fine adjusting unit, division 0.002mm.
Illumination Mirror（Optional）, 3W LED, 12V20W Halogen（Optional）
Accessory Photomicrographic device, dark-
field illumination unit,
microplotter.
3. Installation and use
3.1 Installation
B series microscope with whole package is convenient for installation.
Open the package carton, and insert eyepiece into the eyepiece tube of the head
(eyepiece for monocular microscope has been inserted before packing). If
objectives don’t be fixed, please take objectives out from objectives packing box,

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and drive them into the holes of nosepiece orderly and tightly according to the
multiplying power in order.
Take out the oil bottle and dust cover, and place it properly for convenient use.
▲Please totally take out the devices in the packaging box.
▲Please don’t throw package waste away freely to protect the enviroment.
3.2 Use
3.2.1 Power Supply
Make sure the supply voltage meets the instrument’s requirement, then plug
in the socket. Rotation potentiometer with switch used by the instrument is
for protecting lamp, in case high electric current shock , prolong service time.
3.2.2 Place sample（Fig.2）
 Please place specimen on the stage, and the face with cover glass should
be up, then clip it with clamp.
 Clipping specimen should be careful in order to avoid spoiling glass. Place
specimen on the stage levelly.
 Making the specimen into optical path by adjusting vertical moving knob
and horizontal moving knob.
3.2.3 Condenser（Fig.2）
1．Condenser up-down
Turn the condenser up-down knob to adjust the distance between condenser
front and the specimen, and change the equal illumination to obtain the best
brightness.

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2. Aperture diaphragm adjustment
Rotating the handle of aperture diaphragm of condenser to adjust diaphragm
size can adjust the contrast of the specimen.
In general, aperture diaphragm can be adjusted to 70% or 80% of objective
exit pupil diameter , will get good image with best contrast.
3.2.4 Adjustment of interpupillary distance and diopter (Fig.3)
1. Adjustment of Interpupillary Distance
Please adjusting binocular tube to make left and right field coincide completely .
▲Interpupillar distance is different for everyone, so interpupillar distance
should be adjusted before using binocular microscope.
2. Adjustment of diopter
As focusing for binocular, user should observe right eyepiece with right eye, and
make the right eyepiece clear by focusing adjustment, then observe the left
Fig.3 Adjustment of interpupillary
distance and diopter
Fig.2 Stage and condenser
Clamps swtich knob
Specimen
Stage
vertical
adjusting
knob
Stage
horizonal
adjusting
knob
Condenser
adjusting
handwhee
l
Aperture
diaphragm
lever
Diopter
adjusting
ring
I
nterpupillary
distance dial
Eyepiece
ec
Left side
Right side

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eyepiece, at the same time, adjust the diopter ring of the left eyepiece
tube(diopter difference compensation)to make the image of left eyepiece clear as
same as the right eyepiece.
3.2.5 Focusing unit（Fig.4）
Usually 10X objective with large field of view and long depth of field should
be used for focusing adjustment first.
Take 10X objective into bright path, then observe the specimen with right
eyepiece by right eye and turn coarse focusing knob to find the image, then turn
slowly fine focusing knob to make the image clear.
All objectives of our microscope are precisely adjusted, and approximate
clear image should be found with the other objectives after getting the clear
image with 10X objective. If the image isn’t enough clear with the other
objectives, please turn fine focusing knob slightly to find the satisfactory image.
▲Don’t rotate the left & right coarse and fine focusing knobs in reverse
direction at the same time, if so, the focusing system will be damaged.
▲Don’t directly pull objective to turn nosepiece when user changes the different
objectives,if so, optical quality of microscope possibly be affected.The right way
Fig. 4 Coarse & fine focusing knobs and tension adjusting knobs
Right coarse
focusing knob
Left fine
focusing knob
Left coarse
focusing
knob
Tension
adjusting knob
Right fine
focusing knob

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is to take the tooth-like part of the nosepiece to turn it, and make the objective
into correct position and into the bright path.
3.2.6 Use of tension adjustment knob (Fig.4)
The tension adjustment ring can adjust the tension of the coarse and fine
focusing unit to prevent the stage from sliding down automatically and to
improve the comfort of operation. Rotation clockwise makes tension decrease,
and by contraries, rotation counterclockwise makes tension increase.
3.2.7 Use of oil immersion objective
The 100X objective can be used for observation even if without immersion oil.
But little non-synthetic resin immersion oil or distilled water between the
objective and specimens will fully grasp the objective function , bubbles and
impurities can’t in immersion oil which will affect image.
after adjusting with the 40X objective, adding moderate immersion oil
between the front lens of 100X objective and the cover glass of specimen can
make the image clearer. Please pay attention that air bubble and impurity can’t
be in the immersion oil, otherwise, the image would be affected.
▲After immersion oil used, the oil of specimen and the microscope surface
should be cleaned with absorbent cotton, lens paper, gauze or soft cotton cloth
with moderate mixture of pure industrial alcohol and ether (proportion 1:4)
▲Standard thickness 0.17mm cover glass should be chosen when high times
objective used, and thickness error should be within 0.01mm, otherwise, image
definition wouldbe affected.

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3. 2.8 Use of powersupply
Plug the power line in base socket（Fig.5），plug the other end of power line
into power socket. Adjusting LED lamp brightness by slide the base switch.
Fig.5 Replacement of fuse
3. 2.9 Reflector （Fig.6）
Reflector is optional accessary ,only use without electricity provided.
When use 100X objective ,not recommend reflector for illumination.
Carefully remove the collector from the base and connecting the
reflector with the base.
Turning the reflector around the vertical axis so that the reflective side will
face to the light direction.At the same time around the central axis to rotate the
reflector, reflecting surface and horizontal plane is roughly 45°angle,making the
light totally shining on the specimen.Concave mirror is suitbale for large area
outdoor lighting source ,such as bright sky etc.
3. 2.10 Cautions after use
 Power of microscope should be turned off after using(turning
potentiometer with click voice means be closed）, and the plug should be
Fig.6 Reflector
Potentiomer Socket Fuse holder

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pulled down. If immersion oil used, please clean objective and specimen
soon. Finally, cover microscope with dust cover.
 Please take eyepiece and objectives out from microscope if user will stop
using it for a long time, and place eyepiece and objectives into drier with
drying agent. Cover microscope with dust cover.
4. Maintenance
4.1 Clean
4.1.1 Don’t touch the lens with hand, Dust on lens should be cleaned by soft
brush or absorbent cotton or cleaned by absorbent cotton, lens paper with the
mixture of alcohol and ether (proportion 1:4).
4.1.2 Alcohol and ether all are burnt easily, please take them away from fire.
Be careful for turn on and off power.
4.1.3 Don’t clean painted metal and galvanizing metal with organic solvent
such as alcohol, ether or the mixture of the both. Silicon cloth or soft cleaning
preparation is suggested to clean it.
4.1.4 Plastic should be cleaned by soft cloth with clear water.
4.2 Operational environment
4.2.1 This instrument should be placed in cold ,dry , no acid-base
steam and anti-corrosive gas environment.
4.2.2 The required operation environment :
Indoor temperature: 0 ℃ ~ 40 ℃ Maximum relative humidity: 85%
4.2.3 Dehumidification equipment is suggested to be installed to avoid fungus

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and mist damage when microscope is used in heavy humidity area.
4.2.4 Please pay attention to prevent microscope from violent shake and
vibration in application and in carrying. Don’t drag it on the surface of
worktable to avoid damage microscope and worktable.
4.3 Replacement of bulb
4.3.1 Halogen
Fig. 7
A. Turn off power, and pull out plug.
B. Wait for a while unitil the bulb become cool.
▲ Please be sure that the bulb is cool, then follow by the operations.
C. Lay aside the microscope reliably, unscrew the knurled thumb screw of the
lamphold cover plate on the underside of base.
D. Pull over the lampholder cover plate.
E. Pull out the broken bulb.
F. Hold a new bulb with silk cloth to avoid fingerprint and dust affect bulb
brightness and service life, and insert fully the contact pins into the bulb socket.
1. Loosening screw 2. Open lampholder cover, replace bulb, cover the plate
and tightening the screw.
Fig.7
Reflector
plate
Knurled
locking screw Bulb
Lamphold
er cover
plate

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G. Close the lampholder cover, and tighten the knurled thumb screw.
4.3.2 LED Lamp
A. Cut off the power: Unplug the power in base.
B. Open the base: Laying the microscope steadily on the table,unscrew the
fixed screw and pull out the buttom base cover. (Fig.8)
C. Replacement
Take out LED fastening screw，pull out the two power line’s connector for
LED and replace LED bulb.Insert new line’s connector separately, fixed the
LED bulb on the heat sink base.
D. Install the bottom base：Connect the bottom base with fastening screw and
keep stable.
Fig.8
Fixed screw
Fig.9 LED Lamp
LED Fastening
screw
Holder Heat sink base
LED

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▲ After working for above 10 hours continuously, must cut off the microscope
about 30 minutes.
4.4 Replacement of fuse（Fig.5）
A. Cut off power of microscope, and pull out the plug.
B. Unscrew fuse cap in the back of base, take out old fuse.
C. Replace a new fuse, then screw the fuse cap.
4.5 Microscope not in use
1. Please cut off power, cover the dust cover, and place it in a cool and dry
environment.
2. Objective and eyepiece must be placed in dry container with desiccant.
4.6 Regular checking the microscope for best performance.
▲ To maintain the instrument’s performance, suggest checking instrument
regularly. If any trouble need to repair, please contact with the
anufacturer or local dealer.
5. Troubleshooting
Trouble Causation Remedy
Switch on but bulb dark.
Plug is unreliable Plug in again
Bulb is broken Change bulb
Fuse is broken Change fuse
Bulb is flickering or
brightness is unsteady.
Bulb is unstable Insert it again
Bulb is broken Replacing bulb
Brightness of view field isn’t
enough or uneven.
Bulb specification doesn’t meet the
requirement. Replacing bulb
Brightness is too low. Rotating potentiometer to
adjust.
Objective isn’t in correct position. Make the objective in correct
position.
The size of iris aperture is too small. Adjust the size of iris aperture.

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Trouble Causation Remedy
Lens (objective, eyepiece,
condenser, light
collector)
has dust.
Clean it
Position of condenser is too low. Higher condenser
Image isn’t clear
(contrast or definition isn’t
enough)
Cover glass of specimen doesn’t meet the
requirement.
Use required thickness cover
glass. (0.17mm)
Cover glass of specimen isn’t in up
direction. Place specimen correctly.
Surface of objective lens is dirty
(especially it is easy for the front lens of
40X objective to dip in immersion oil).
Clean it
Immersion oil isn’t used for 100X
objective (oil). Use immersion oil
Immersion oil doesn’t meet the
requirement.
Use immersion oil supplied
by producer.
There is bubble in immersion oil. Clear the bubble way
Size of iris aperture isn’t proper. Adjust the size of iris aperture.
Position of condenser is too low. Readjust the position of
condenser.
One side of image is dark or
image is moving as focusing.
Objective isn’t in correct position. Make the objective in
correct position.
Specimen isn’t placed correctly. Place specimen levelly on
stage and clip it with clamp.
Objective touches specimen
as changing low times
objective to high times
objective.
Cover glass of specimen isn’t in up
direction. Place specimen correctly.
Cover glass doesn’t meet the requirement.
Use required thickness cover
glass. (0.17mm)
Image observed by two eyes
aren’t in superposition
entirely.
Interpupilary distance isn’t adjusted
correctly .
Adjust interpupilary
distance according to two
eyes.
It is easy for eyes to be tired
during observing. Diopter isn’t adjusted correctly. Readjust diopter