OPTO-EDU A14.1065 User manual

A14.1065
Inverted Biological Phase Contrast Microscope
A16.1065
Inverted LED Fluorescent Microscope, B,G,U
Instruction Manual
This instruction manual is intended for users of the Inverted Series Biological Microscope
A16.1065. To ensure safety use, perform the best performance and to familiarize yourself with
the use of this microscope, please read this manual carefully before operating this product.

II
Contents
Before Using ……………………………………………………………………
1
Safety Symbol…………………………………………………………………
1
Safety Precautions ……………………………………………………………
1
Care and Maintenance…………………………………………………………
2
Chapter 1 Introduction …………………………………………………………
4
1.1 Technical Specifications …………………………………………………
4
1.1.1 Main Technical Specifications and Configuration……………………
4
1.1.2 Objective Parameters…………………………………………………
5
1.1.3 Electrical Parameters…………………………………………………
5
1.2 Components ………………………………………………………………
6
Chapter 2 Overview of Agencies ………………………………………………
8
Chapter 3 Regulation and Operation …………………………………………
10
3.1 Turning the Power on ……………………………………………………
10
3.2 Adjusting the Illumination Brightness……………………………………
10
3.3 Specimen Placement and Stage Knob Adjustment………………………
10
3.3.1 Mechanical Stage……………………………………………………
10
3.3.2 Pallet…………………………………………………………………
11
3.3.3 Auxiliary Stage………………………………………………………
11
3.4 Adjusting the Interpupillary Distance………………………………………
11
3.5 Focusing on the Specimens ………………………………………………
12
3.5.1 Focus Knobs…………………………………………………………
12
3.5.2 Focus Knob Tight Adjustment ………………………………………
12
3.6 Adjusting the Diascopic Illumination Aperture Diaphragm………………
13
3.7 Switching the Objective……………………………………………………
13
3.8 Operation the Phase Contrast Microscope…………………………………
14
3.9 Operating the Episcopic Illumination Microscopy………………………………
15
3.9.1 Eprscopic Illumination………………………………………………
15
3.9.2 Fluorescence Filter Cube Turret ……………………………………
15
3.9.3 Filter Cube Switching Unit …………………………………………
15
3.9.4 Fluorescence Filter Cube……………………………………………
16
3.9.5 Fluorescence LED Unit ……………………………………………
16

III
3.10 Operating the LCD ……………………………………………………
17
3.10.1 The status of LCD …………………………………………………
17
3.10.2 Operating the illumination switch ………………………………
17
Chapter 4Assembly………………………………………………………………
18
4.1 Installation diagram ……………………………………………………
18
4.2 Installation Steps ………………………………………………………
19
4.2.1 Installing the Condenser ……………………………………………
19
4.2.2 Installing the Phase Contrast Slider …………………………………
19
4.2.3 Installing the Moving Ruler …………………………………………
19
4.2.4 Installing the Objective ………………………………………………
20
4.2.5 Installing the Eyepiece ………………………………………………
20
4.2.6 Installing the Camera…………………………………………………
20
4.3 Assembly Episcopic Illumination Microscopy…………………………………
21
4.3.1 Installing the Filter Cube ……………………………………………
21
4.3.2 Installing the Fluorescence LED Unit ………………………………
21
4.3.3 Replacing the Fuse …………………………………………………
21
Chapter 5 Maintenance and Storage……………………………………………
22
5.1 Cleaning …………………………………………………………………
22
5.1.1 Cleaning the Lenses …………………………………………………
22
5.1.2 Cleaning Parts other than Lenses ……………………………………
22
5.1.3 Cleaning off Immersion Oil …………………………………………
23
5.1.4 Decontaminating this Product ………………………………………
23
5.2 Storage ……………………………………………………………………
23
Chapter 6 Troubleshooting ……………………………………………………
24
6.1 Optical System ……………………………………………………………
24
6.2 Mechanical Parts …………………………………………………………
24
6.3 electrical Parts………………………………………………………………
25

Before Using A16.1065
Safety Symbol
Symbol
Description
The surface is hot and cannot be touched with bare hands.
Please read this manual carefully before using the product.
Incorrect usage can cause personal injury or property damage.
The main power switch is turned on.
The main power switch is turned off.
Adjustment direction of illumination.
Ground connection.
Up and down light switch.
Light switch.
Through the CE certification.
Lever in，Bino：Trino=100：0
Lever out，Bino：Trino=20：100
Safety Precautions
1. Be careful when opening box, prevent lens glass to stick to fingerprint,
perspiration other factors influence observation, prevent lens and other
attachment drop damaged.
2. Avoid placing the microscope in place with direct sunlight, high temperature
or high humidity, dust, and strong vibration. Ensure that the stage is flat,
horizontal and strong enough.
3. If a bacterial solution or water spatters spill on the stage, objective or
observing tube, immediately unplug the power cord. Then,wipe off any
solution or water with to keep the microscope dry. Otherwise, it could damage
the instrument.
4. To avoid blocking the natural convection air for cooling, make sure the left,
right, top, and rear of the microscope are at least 10 centimeters away from
the wall and other objects.

2
A16.1065
5. Grounding the machine to avoid lightning strike.
6. To ensure safety, always make sure the main switch is in "O"
(disconnecting) and cut off the power while waiting for the LED lights and
the lamp room to cool completely. Input voltage check: the input voltage
indicated on the back of the microscope is consistent with the supply voltage,
otherwise the microscope will be seriously damaged.
7. Use the our company dedicated wire.
8. When carrying this product, one hand should firmly hold it by gripping the
recess in the front lower part of the main body and the other holds the recess
in the rear of the main body.
★Do not hold any other parts (such as the upper part of the illumination pillar, focus
knobs, eyepiece tube, or stage) when carrying the microscope. Doing so might result in
dropping or failure of this microscope.
Care and Maintenance
1. All lenses have been calibrated, please do not disassemble by yourself.
2. The structure of objective converter and coarse and fine focus mechanism
are precise, please do not disassemble easily.
3. The instrument should be kept clean, have to regular removal of dust, special
attention should be paid to do not contaminate the optical parts when
cleaning.
4. The stain of the lens, such as fingerprints and grease can be wiped with a
small amount of ethyl ether (70%) and alcohol (30%) mixed solution.
Recess in the rear
Recess in the front lower part
F
F

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A16.1065
5. Do not use organic solvents to clean the non-optical parts of the microscope.
Please use neutral detergents when cleaning.
6. When useing, if the microscope be wet liquid, should immediately cut off
the power and dry.
7. Do not disassemble any part of the microscope, which will affect the
function or reduce the performance of the microscope.
8. The instrument should be placed indoor with cool and dry, covered the dust
cover when not in use. Cover up, must wait for the lamp room to cool down.
9. The microscope usage environmental requirements:
a) For indoor use;
b) The environment temperature range: 10 ℃~ 35 ℃;
c) Maximum relative humidity: 80% at 31 ℃, linear temperature down to
34 ℃is 70%, 60% at 37 ℃, 50% at 40 ℃.
10. Microscope to store and transport environment requirements:
a) the environment temperature range: - 40 ℃~ + 70 ℃
b) relative humidity range: 10% ~ 100%
c) atmospheric pressure range: 500 hpa ~ 1060 hpa
Because the solvent such as ether and alcohol are extremely flammable, do not
operate power switch of various electrical equipment when using, and keep away
from open fire, please ensure indoor ventilation.

4
Chapter 1 Introduction A16.1065
1.1 Technical Specifications
1.1.1 Main technical specifications and configuration
Device
Technical Specifications
A14.1065
A16.1065
Eyepiece
Extra Wide Field Eyepiece 10×/22
●
●
Field Eyepiece 15×/16
○
○
Field Eyepiece 20×/12
○
○
Observation head
Seidentopf Viewing Head, alterable angle,
Interpupillary 48-75mm
●
●
Objective
Infinity plane objective 4×
●
○
Infinity plane objective 10×
○
○
Infinity plane objective 20×
○
○
Infinity plane objective40×
○
○
Phase contrast objective 4×
○
○
Phase contrast objective 10×
●
○
Phase contrast objective 20×
●
○
Phase contrast objective 40×
●
○
Infinity plane semi-apochromatic objective 10×
—
●
Infinity plane semi-apochromatic objective 20×
—
●
Infinity plane semi-apochromatic objective 40×
—
●
Nosepiece
Quintuple Nosepiece
●
●
Focusing mechanism
up 7mm, down 1.5mm，To limit up to 18.5mm
●
●
stage
250mm×170mm
●
●
Moving stage
Y 80mm，X 128mm
●
●
Plain stage
Two plain stage
○
○
Condenser
NA=0.3
●
●
illumination
Transmitted illumination：3WLED
●
●
Fluorescent illumination：（ B、G、U）3WLED
—
●
Fluorescent illumination：V 3WLED
—
○
Emboss contrast slider
contrast slider
○
○
Camera mount
C-Mount 1×
●
●
C-Mount 0.7×
○
○
C-Mount 0.5×
○
○
LCD
●
●
Note：● Standard component, ○ Choose component,―Inapplicability。

5
A16.1065
Objective Choose component
No.
Technical Specifications
1
Infinity plane semi-apochromatic objective（adjustable）20×、40×、60×
2
Infinity plane semi-apochromatic contrast objective（adjustable）20×、40×、60×
3
Infinity plane semi-apochromatic contrast objective 4×、10×、20×、40×、60×
1.1.2 Objective parameters
Type
A14.1065
A16.1065
Magnification
4×
10×
20×
40×
10×
20×
40×
numerical aperture(N.A)
0.10
0.25
0.40
0.60
0.30
0.45
0.60
Work distance (mm）
30
10.2
12
2.2
7.4
8
3.6
Cover-glass thickness
0.17
0.17
0.17
0.17
1.2
1.2
1.2
Conjugate distance(mm)
∞
∞
∞
∞
∞
∞
∞
1.1.3 Electricity parameter
Item
A14.1065
A16.1065
1
Input voltage
AC100-240V; 50/60 Hz
2
Fuse Size
T500 mA; 250 V
3
LED light
3W S-LED
4
Fluorescence LED light (B light）
—
wavelength is 485nm at 3W
5
Fluorescence LED light (G light)
—
wavelength is 520nm at 3W
6
Fluorescence LED light (U light)
—
wavelength is 360nm at 3W

6
A16.1065
1.2 Components
The microscope A16.1065 components ( following diagrams) .
A16.1065
Viewing head
Camera mount
LCD
Mainframe
Nosepiece
Stage
Condenser
Objective
Contrast slider
Moving stage
Eyepiece

7
A16.1065
The microscope A16.1065-FL components ( following diagrams).
A16.1065-FL
Viewing head
Camera mount
LCD
Mainframe
Fluorescence
illumination
Stage
Condenser
Filter Cube turret
Fluorescent baffle
Eyepiece
Nosepiece
Objective
Moving stage
Upper lighting
shading part
Contrast slider