OPTO-EDU A14.1065 User manual

A14.1065
Inverted Biological Phase Contrast Microscope
A16.1065
Inverted LED Fluorescent Microscope, B,G,U
Instruction Manual
This instruction manual is intended for users of the Inverted Series Biological Microscope
A16.1065. To ensure safety use, perform the best performance and to familiarize yourself with
the use of this microscope, please read this manual carefully before operating this product.

II
Contents
Before Using ……………………………………………………………………
1
Safety Symbol…………………………………………………………………
1
Safety Precautions ……………………………………………………………
1
Care and Maintenance…………………………………………………………
2
Chapter 1 Introduction …………………………………………………………
4
1.1 Technical Specifications …………………………………………………
4
1.1.1 Main Technical Specifications and Configuration……………………
4
1.1.2 Objective Parameters…………………………………………………
5
1.1.3 Electrical Parameters…………………………………………………
5
1.2 Components ………………………………………………………………
6
Chapter 2 Overview of Agencies ………………………………………………
8
Chapter 3 Regulation and Operation …………………………………………
10
3.1 Turning the Power on ……………………………………………………
10
3.2 Adjusting the Illumination Brightness……………………………………
10
3.3 Specimen Placement and Stage Knob Adjustment………………………
10
3.3.1 Mechanical Stage……………………………………………………
10
3.3.2 Pallet…………………………………………………………………
11
3.3.3 Auxiliary Stage………………………………………………………
11
3.4 Adjusting the Interpupillary Distance………………………………………
11
3.5 Focusing on the Specimens ………………………………………………
12
3.5.1 Focus Knobs…………………………………………………………
12
3.5.2 Focus Knob Tight Adjustment ………………………………………
12
3.6 Adjusting the Diascopic Illumination Aperture Diaphragm………………
13
3.7 Switching the Objective……………………………………………………
13
3.8 Operation the Phase Contrast Microscope…………………………………
14
3.9 Operating the Episcopic Illumination Microscopy………………………………
15
3.9.1 Eprscopic Illumination………………………………………………
15
3.9.2 Fluorescence Filter Cube Turret ……………………………………
15
3.9.3 Filter Cube Switching Unit …………………………………………
15
3.9.4 Fluorescence Filter Cube……………………………………………
16
3.9.5 Fluorescence LED Unit ……………………………………………
16

III
3.10 Operating the LCD ……………………………………………………
17
3.10.1 The status of LCD …………………………………………………
17
3.10.2 Operating the illumination switch ………………………………
17
Chapter 4Assembly………………………………………………………………
18
4.1 Installation diagram ……………………………………………………
18
4.2 Installation Steps ………………………………………………………
19
4.2.1 Installing the Condenser ……………………………………………
19
4.2.2 Installing the Phase Contrast Slider …………………………………
19
4.2.3 Installing the Moving Ruler …………………………………………
19
4.2.4 Installing the Objective ………………………………………………
20
4.2.5 Installing the Eyepiece ………………………………………………
20
4.2.6 Installing the Camera…………………………………………………
20
4.3 Assembly Episcopic Illumination Microscopy…………………………………
21
4.3.1 Installing the Filter Cube ……………………………………………
21
4.3.2 Installing the Fluorescence LED Unit ………………………………
21
4.3.3 Replacing the Fuse …………………………………………………
21
Chapter 5 Maintenance and Storage……………………………………………
22
5.1 Cleaning …………………………………………………………………
22
5.1.1 Cleaning the Lenses …………………………………………………
22
5.1.2 Cleaning Parts other than Lenses ……………………………………
22
5.1.3 Cleaning off Immersion Oil …………………………………………
23
5.1.4 Decontaminating this Product ………………………………………
23
5.2 Storage ……………………………………………………………………
23
Chapter 6 Troubleshooting ……………………………………………………
24
6.1 Optical System ……………………………………………………………
24
6.2 Mechanical Parts …………………………………………………………
24
6.3 electrical Parts………………………………………………………………
25

Before Using A16.1065
Safety Symbol
Symbol
Description
The surface is hot and cannot be touched with bare hands.
Please read this manual carefully before using the product.
Incorrect usage can cause personal injury or property damage.
The main power switch is turned on.
The main power switch is turned off.
Adjustment direction of illumination.
Ground connection.
Up and down light switch.
Light switch.
Through the CE certification.
Lever in，Bino：Trino=100：0
Lever out，Bino：Trino=20：100
Safety Precautions
1. Be careful when opening box, prevent lens glass to stick to fingerprint,
perspiration other factors influence observation, prevent lens and other
attachment drop damaged.
2. Avoid placing the microscope in place with direct sunlight, high temperature
or high humidity, dust, and strong vibration. Ensure that the stage is flat,
horizontal and strong enough.
3. If a bacterial solution or water spatters spill on the stage, objective or
observing tube, immediately unplug the power cord. Then,wipe off any
solution or water with to keep the microscope dry. Otherwise, it could damage
the instrument.
4. To avoid blocking the natural convection air for cooling, make sure the left,
right, top, and rear of the microscope are at least 10 centimeters away from
the wall and other objects.

2
A16.1065
5. Grounding the machine to avoid lightning strike.
6. To ensure safety, always make sure the main switch is in "O"
(disconnecting) and cut off the power while waiting for the LED lights and
the lamp room to cool completely. Input voltage check: the input voltage
indicated on the back of the microscope is consistent with the supply voltage,
otherwise the microscope will be seriously damaged.
7. Use the our company dedicated wire.
8. When carrying this product, one hand should firmly hold it by gripping the
recess in the front lower part of the main body and the other holds the recess
in the rear of the main body.
★Do not hold any other parts (such as the upper part of the illumination pillar, focus
knobs, eyepiece tube, or stage) when carrying the microscope. Doing so might result in
dropping or failure of this microscope.
Care and Maintenance
1. All lenses have been calibrated, please do not disassemble by yourself.
2. The structure of objective converter and coarse and fine focus mechanism
are precise, please do not disassemble easily.
3. The instrument should be kept clean, have to regular removal of dust, special
attention should be paid to do not contaminate the optical parts when
cleaning.
4. The stain of the lens, such as fingerprints and grease can be wiped with a
small amount of ethyl ether (70%) and alcohol (30%) mixed solution.
Recess in the rear
Recess in the front lower part
F
F

3
A16.1065
5. Do not use organic solvents to clean the non-optical parts of the microscope.
Please use neutral detergents when cleaning.
6. When useing, if the microscope be wet liquid, should immediately cut off
the power and dry.
7. Do not disassemble any part of the microscope, which will affect the
function or reduce the performance of the microscope.
8. The instrument should be placed indoor with cool and dry, covered the dust
cover when not in use. Cover up, must wait for the lamp room to cool down.
9. The microscope usage environmental requirements:
a) For indoor use;
b) The environment temperature range: 10 ℃~ 35 ℃;
c) Maximum relative humidity: 80% at 31 ℃, linear temperature down to
34 ℃is 70%, 60% at 37 ℃, 50% at 40 ℃.
10. Microscope to store and transport environment requirements:
a) the environment temperature range: - 40 ℃~ + 70 ℃
b) relative humidity range: 10% ~ 100%
c) atmospheric pressure range: 500 hpa ~ 1060 hpa
Because the solvent such as ether and alcohol are extremely flammable, do not
operate power switch of various electrical equipment when using, and keep away
from open fire, please ensure indoor ventilation.

4
Chapter 1 Introduction A16.1065
1.1 Technical Specifications
1.1.1 Main technical specifications and configuration
Device
Technical Specifications
A14.1065
A16.1065
Eyepiece
Extra Wide Field Eyepiece 10×/22
●
●
Field Eyepiece 15×/16
○
○
Field Eyepiece 20×/12
○
○
Observation head
Seidentopf Viewing Head, alterable angle,
Interpupillary 48-75mm
●
●
Objective
Infinity plane objective 4×
●
○
Infinity plane objective 10×
○
○
Infinity plane objective 20×
○
○
Infinity plane objective40×
○
○
Phase contrast objective 4×
○
○
Phase contrast objective 10×
●
○
Phase contrast objective 20×
●
○
Phase contrast objective 40×
●
○
Infinity plane semi-apochromatic objective 10×
—
●
Infinity plane semi-apochromatic objective 20×
—
●
Infinity plane semi-apochromatic objective 40×
—
●
Nosepiece
Quintuple Nosepiece
●
●
Focusing mechanism
up 7mm, down 1.5mm，To limit up to 18.5mm
●
●
stage
250mm×170mm
●
●
Moving stage
Y 80mm，X 128mm
●
●
Plain stage
Two plain stage
○
○
Condenser
NA=0.3
●
●
illumination
Transmitted illumination：3WLED
●
●
Fluorescent illumination：（ B、G、U）3WLED
—
●
Fluorescent illumination：V 3WLED
—
○
Emboss contrast slider
contrast slider
○
○
Camera mount
C-Mount 1×
●
●
C-Mount 0.7×
○
○
C-Mount 0.5×
○
○
LCD
●
●
Note：● Standard component, ○ Choose component,―Inapplicability。

5
A16.1065
Objective Choose component
No.
Technical Specifications
1
Infinity plane semi-apochromatic objective（adjustable）20×、40×、60×
2
Infinity plane semi-apochromatic contrast objective（adjustable）20×、40×、60×
3
Infinity plane semi-apochromatic contrast objective 4×、10×、20×、40×、60×
1.1.2 Objective parameters
Type
A14.1065
A16.1065
Magnification
4×
10×
20×
40×
10×
20×
40×
numerical aperture(N.A)
0.10
0.25
0.40
0.60
0.30
0.45
0.60
Work distance (mm）
30
10.2
12
2.2
7.4
8
3.6
Cover-glass thickness
0.17
0.17
0.17
0.17
1.2
1.2
1.2
Conjugate distance(mm)
∞
∞
∞
∞
∞
∞
∞
1.1.3 Electricity parameter
Item
A14.1065
A16.1065
1
Input voltage
AC100-240V; 50/60 Hz
2
Fuse Size
T500 mA; 250 V
3
LED light
3W S-LED
4
Fluorescence LED light (B light）
—
wavelength is 485nm at 3W
5
Fluorescence LED light (G light)
—
wavelength is 520nm at 3W
6
Fluorescence LED light (U light)
—
wavelength is 360nm at 3W

6
A16.1065
1.2 Components
The microscope A16.1065 components ( following diagrams) .
A16.1065
Viewing head
Camera mount
LCD
Mainframe
Nosepiece
Stage
Condenser
Objective
Contrast slider
Moving stage
Eyepiece

7
A16.1065
The microscope A16.1065-FL components ( following diagrams).
A16.1065-FL
Viewing head
Camera mount
LCD
Mainframe
Fluorescence
illumination
Stage
Condenser
Filter Cube turret
Fluorescent baffle
Eyepiece
Nosepiece
Objective
Moving stage
Upper lighting
shading part
Contrast slider

8
Chapter 2 Overview of Agencies A16.1065
1
Condenser aperture adjustment knob
7
Condenser brightness
2
Condenser rotating knob
8
Fine focus knob
3
Condenser adjustment knob
9
Coarse focus knob
4
aperture diaphragm adjusting knob
10
Power indicator
5
Nosepiece
11
Camera mount
6
Power switch
A14.1063
4
1
2
3
5
6
9
7
8
10
11

9
A16.1065
1
Bracket horizontal movement knob
2
Bracket vertical movement knob
3
Fluorescence cube turret
A16.1063
3
1
2

10
Chapter 3 Regulation and Operation A16.1065
3.1 Turning the Power on
Turn on the power and turn the main switch on
the back of the microscope body to "-" (on).
3.2Adjusting the Illumination Brightness
As shown in the direction of rotation, the light
source is enhanced, whereas the light source
weakened.
3.3 Specimen Placement and Stage Knob
Adjustment
Load the slide holder into a mechanical stage,
and the cover glass slides downward slowly
into the grooves of the slide plate.
Rotate the stage knob to place the sample to
the desired position.
CAUTION
Be careful when changing the objective. The
objective may collide with the specimen,
because of the short working distance of
specimen observation.
3.3.1 Movving stage
The Movving stage is mounted and used on
the plain stage. The specimen can be moved in
the X-axis and Y-axis directions by operating
the stage knobs.
A 128×80 mm microplate can be placed on
the Movving stage. Attaching the different
specimen holders enables you to observe
various types of specimens.
The upper is the vertical knob, and rotates in
the direction as shown in the figure, the stage
will move forward in the vertical direction.
The lower is the horizontal knob rotating in the
direction shown in the figure. The stage will
move to the left in the horizontal direction.
Power switch

11
A16.1065
3.3.2 Holders
There are a variety of holders to choose from.
NO.
Model
1
Terasaki microplate
2
Universal holder
3
54mm Petri dish holder/ Slide holder
4
60 mm Petri dish holder/ Slide holder
5
90 mm Petri dish holder
3.3.3Auxiliary stage
Connect the auxiliary stage to the left and
right of the stage to expand the stage
width. After connecting the auxiliary
stage, the stage width is 300 mm.
3.4Adjusting the Interpupillary
Distance
By rotating the binocular part while
looking through the eyepieces, adjust the
distance between the eyepieces so that the
right and left view fields overlap to a
single image from eyepieces
Auxiliary stage

12
A16.1065
3.5 Focusing on the Specimens
3.5.1 Focus knob
To adjust the focus，rotate the focus knobs on
the right and left sides of the microscope to
move the objective up and down.
The figure to the right illustrates the
relationship between the rotational direction
of the focus knobs and the vertical notion of
the objective.
Focus travel: from the focal point, up to 7mm
and down to 1.5mm, and the limit can be up
to 18.5mm. (As the right figure, spin out the
limit screw for about 4 mm trip to limit)
The traveling distance of the objective for
each knob is as follows:
Rotation of knob
Distance traveled
by objective
Fine focus knob: 1 rotation
0.2 mm
Coarse focus knob: 1 rotation
37.7 mm
CAUTION
Never attempt the following operations, as
they might result in product malfunction:
◆Rotating the left and right focus knobs in
opposite directions.
◆Rotating the coarse and fine focus knobs
past their limit.
3.5.2 Focus knob tight adjustment
The focus knob become tighter, when
rotating direction of the tightness adjustment
knob is shown as figure 8. Otherwise become
more relaxed
Tightness adjustment knob
Coarse focus knob
Fine focus knob
limit screw

13
A16.1065
3.6Adjusting the Transmitted
Illumination Aperture Diaphragm
Insert a centering telescope in the eyepiece
tube, adjust the focal length, and view the
objective pupil plane (a bright circle) and the
aperture diaphragm image. Adjust the
aperture diaphragm so that the size of the
diaphragm image is 70-80% of the size of
the objective pupil plane.
Aperture diaphragm is designed for the
adjustment of numerical aperture, not the
dimmer. Typically, adjusting the aperture
diaphragm to 70% to 80% of the pupil of the
objective will result in an appropriate
contrast and a favorable image. The iris
aperture adjusting handle is marked with the
aperture of the diaphragm.
3.7 Nosepiece
Turn the nosepiece until it clicks in place,
stays in the specified position, can switch the
objective.
Before rotating the converter, check the
objective height to prevent collision with
specimens or stage.
Normally, the objective is mounted by
rotating the nosepiece clockwise (from
above the nosepiece) increasing
magnification in turn.
CAUTION
Be careful when changing the objective.
The objective may collide with the
specimen, because of the short working
distance of specimen observation.
Aperture diaphragm
open/ close lever
Aperture diaphragm image
Objective pupil plane
70-80%
100%

14
A16.1065
3.8 Operation the Phase Contrast Microscope
Phase contrast microscopy requires phase contrast (PH) objectives and PH sliders.
If PH Slider is used, it is possible to center the annular diaphragm. Turn the centering
screw of the PH slider to merge the annular diaphragm image with the phase ring
image in the objective.
Decentering the phase ring and the PH annular diaphragm
Basically, the phase ring and the PH annular diaphragm should be adjusted so that they
are concentric. However, slightly decentering them will produce a shadowing effect,
resulting in a three-dimensional image.
Annular diaphragm
Centering hold
(on the side)
Hollow position
Centering hold
(on the side)
Hexagonal
screwdriver
Centering screws
Hexagonal
screwdriver

15
A16.1065
3.9Operating the Episcopic Illumination Microscopy (for the 410-FL Only)
3.9.1 Episcopic illumination （for
A16.1065-FL）
Changing the illumination switch to switch
between episcopic illumination LED and
diascopic illumination fluorescent LED
mode, see section 4.3.2.
Fluorescent LED illumination will turn
weaker, when rotating direction of
illumination brightness adjuster as right
figure shown, whereas light source will turn
brighter.
3.9.2 Fluorescence cube turret
The fluorescence cube turret reflects the
illumination light from the fluorescence LED
unit into the objective. This turret allows you
to attach up to three filter cubes (each with a
built-in dichroic mirror) and switch the filter
cube by rotating the filter cube switching unit.
3.9.3 Filter cube switching unit
Rotating the filter cube switching unit
switches the filter cube. When the filter cube
is switched, the fluorescence LED unit is
switched automatically.
Fluorescent positions (1 to 3) and a
bright-field position（）are alternately
allocated to the turret. The turret clicks each
time a filter cube is brought to a fluorescent
or a bright-field position. Address of the filter
cube that is in the optical path is indicated on
the right and left of the filter cube switching
unit. When switching the filter cube, check
that the turret clicks and the address
indications match the intended filter cube.
Illumination LED
ON/OFF switch
Illumination
brightness adjuster
Filter cube mounting port
Filter cube switching unit
Address indication
for filter cube
Address indication
for filter cube

16
A16.1065
position, seen from the front of the
microscope: If you select the bright-field
position, the filter cube is removed from the
optical path. The three bright-field positions
have the same function.
Rotate the turret so that the fluorescence
positions indicated by the arrows on the left
and right are the same (all points to “1” in the
right figure), indicating that the filter cube
has been switched into the optical path.
★One-to-one corresponding relationship
between filter cube sign, fluorescent
LED light signs and filter cube should
be paid attention to, when using. (see
section 4.3.2 for the position of
fluorescent LED lights)
3.9.4 Fluorescence Filter Cube
A filter cube consists of three types of optical
components: an excitation filter (BP filter), a
barrier filter (BA filter), and a dichroic mirror
(DM).
Referring to the following items as a guide,
select a desired combination of filter cubes
according to the characteristics of the
specimen and fluorophore.
• You can select a combination of an
excitation filter and a barrier filter even if
you are using the same excitation method.
• Excitation filters, barrier filters, and
dichroic mirrors can be purchased separately.
• Excitation filters are exposed to strong light
during operation and will deteriorate over
time. Replace the filter as appropriate.
3.9.5 Fluorescent LED Unit
The 410-FL allows you to mount up to three
different fluorescence LED units as the light
source for episcopic fluorescence
microscopy.
When the fluorescence cube turret is rotated,
the fluorescence LED unit is switched
automatically. Therefore, mount the
fluorescence LED unit at the same address as
the filter cube to be used.
Dichroic mirror
SIGN

17
A16.1065
3.10 Operating the LCD（for A16.1064 series）
3.10.1 The status of LCD
3.10.2 Operating the illumination switch
⚫Click brightness adjuster：becoming standby mode，it’s“SLEEP”in the screen。
Click again，it becomes working mode。
⚫Press brightness adjuster three seconds：setting time. First minute scale figures
flash, then click the button, hour scale figures become flashing. When the time figures
flash three times, it means setting successful.
⚫Double-click：locking brightness or unlocking. When it is locking, you can not
adjust brightness every objective unless double click again.
⚫rotating：changing brightness。
⚫Press and rotating up meantime：Transmitted illumination。
⚫Pressing and rotating down meantime：Fluorescent illumination。
Start & working mode
Standby
Lock
Time setting
SLEEP
TIME