OPTO-EDU A12.0910 User manual

A12.0910
Research Scientific Laboratory Microscope,
Semi-APO, Upgrade BF+DF+PL+PH+FL+DIC
Instruction Manual
This instruction manual is for the operation guide, troubleshooting and
maintenance to the A12.0910 series biological microscope. Please study this
manual thoroughly before operating, and keep it with the instrument. The
manufacturer reserves the rights to the modifications by technology
development. On the basis of operation ensured, technical specifications
may be subject to changes without notice.

Contents A12.0910
Before Use
1. Components..................................................................................................................................1
2. Assembling....................................................................................................................................5
2-1 Assembling Scheme ............................................................................................................5
2-2 Assembling Steps................................................................................................................7
3. Operation....................................................................................................................................10
3-1 Set Illumination ................................................................................................................10
3-2 Place the Specimen Slide..................................................................................................10
3-3 Select the Light Path.........................................................................................................11
3-4 Adjust Focusing................................................................................................................11
3-5 Adjust the Focusing Tension ............................................................................................12
3-6 Adjust the Interpupillary Distance ...................................................................................12
3-7 Use the Eye-cap................................................................................................................12
3-8 Adjust Stage......................................................................................................................12
3-9 Center the Condenser.......................................................................................................14
3-10 Adjust the Field Diaphragm...........................................................................................14
3-11 Adjust the Aperture Diaphragm .....................................................................................15
3-12 Use the Color Filter .......................................................................................................15
3-13 Replace the Fuse ............................................................................................................16
3-14 Assemble and Use the TV Device...................................................................................16
4. Assembling and Operation of Fluorescence Illuminator............................................................17
4-1 Assemblage Steps .............................................................................................................17
4-2 Operation .........................................................................................................................21
5. Troubleshooting..........................................................................................................................26
5-1 Solutions of A12.0910 Series Biological Microscope Problem........................................26
5-2 Solutions of Mercury Bulb Fluorescent Device Problem .................................................28

Before Use A12.0910
1. Operation Notice 1. As the microscope is a high precision instrument,
always operate it with care, and avoid physical shake
during the operation.
2. Do not expose the microscope in the sun directly,
either not in the high temperature, damp, dust or acute
shake. Make sure the worktable is flat and horizontal.
Following environment is required when operating:
Indoor temperature: 5 ℃ ～ 40 ℃, Max relative
humidity: 80%.
3. When moving the microscope, use both hands to
hold its back hand-clasping ①and the front base ②,
and lay it down carefully (see Fig. left).
★It will damage the microscope by holding the
stage, focusing knob, head or light source when
moving.
4. When working, the surface of light source will be
very hot. Make sure there is enough room for the heat
dissipating around the light source.
5. Connect the microscope to the ground to avoid
lightning strike.
6. For safety, make sure the power switch is at “O”
(off) and power it off before replacing the bulb or fuse
(see Fig. left).
7. Wide voltage range is supported as 100~240V.
Additional transformer is not necessary. Make sure the
voltage is in this range.
8. Use the special wire supplied by our company.
2. Maintenance
1. Wipe the lens gently with a soft tissue. Carefully wipe off the oil marks and fingerprints on the
lens surfaces with a tissue moistened with a small amount of 3:7 mixture of alcohol and ether or
dimethylbenzene.
★As the alcohol and ether is flammable, don’t place these chemical near to fire or fire
source. For example, when turning on or turning off the electrical device, please use these
chemical in a ventilated place.
2. Don’t use organic solution to wipe the surfaces of the other components. Please use the neutral
detergent if necessary.
3. If the microscope is damped by liquid, please power it off immediately and wipe it dry.
4. Never disassemble the microscope, otherwise the performance will be affected or the
instrument will be damaged.
5. After using, cover the microscope with a dust cover.
3. Safety Sign
Sign
Signification
The surface gets hot and don’t touch it with bare hand.
Study the instructions before use. Unsuitable operation would lead to
person hurt or instrument faulty.
|
Main switch ON
O
Main switch OFF

1
1. Components A12.0910
A12.0910 Series Biological Microscope Components
Objective
Trinocular head
Light Path
Selecting Pole
Inner
Hexagon
Spanner
Body
Voltage
Indicator
Light
Source
Group
Reset Button
For Intensity
Mechanical
Stage
Condenser
Illuminator
Light
Adjustment
Knob
Tension
Adjustment
Knob
Switch Lever
For Filters
Y-axis Moving
Knob
X-axis Moving
Knob

2
A12.0910
Fine
Focusing
Knob
Coarse
Focusing
Knob
Condenser
up-down knob
Dust Cover
Coarse Adjusting
Limit Ring
Nosepiece
Objective

3
A12.0910
A12.0910FL Series Biological Fluorescence Microscope Components
Trinocular
head
Flashboard
knob
XY Body
Vertical centering
Screw
Blinkers
Board
Field Diaphragm
Adjusting Pole
Aperture Diaphragm
Adjusting Pole
Lens Focusing Knob
Diaphragm
Centering Screw
Horizontal
centering Screw
Power Supply Box
Vertical
Fluorescence
Illuminator

4
A12.0910
ND Filter Slot

5
2. Assembling A12.0910
2-1 Assembling Scheme
Following is theAssembling Scheme, and the numbers denote the assembling order.
★Before assembling, make sure there is no dust, dirt or other matters to affect the
assembly. Assemble carefully and do not scrap any part or touch the glass surface.
1. A12.0910 Series Biological Microscope Assembling Scheme

6
A12.0910
2. A12.0910 Series Biological Fluorescence Microscope Assembling Scheme

7
A12.0910
2-2 Assembling Steps
2-2-1 Assemble and Replace the Halogen Bulb
(1) Loose lock-screw ①completely by M4 spanner ,
then take off the cover group. (See Fig. 1)
(2) Open the bulb lock piece②, and take the bulb③
with clean glove or soft tissue, insert the bulb pins④
into the jack on the bulb holder⑤, the bulb should be
vertical after assembly. (See Fig. 2)
(3) Loose the bulb lock piece②, lock the bulb and
install the cover group.
★Don’t touch the bulb with fingers. If there is a
fingerprint left on the bulb, please wipe it with
clean soft cloth.
★Bulb selected only: 12V/100W Halogen bulb
(Philips 7724).
★Before replacing the bulb, make sure to cut off the
main power and wait for both the bulb base and
bulb cooling down.
2-2-2 Assemble the Light Source Group
Push the light source holder①into the body holder,
Make the light source group to be horizontal with the
body group and lock the screw②. (See Fig. 3)
2-2-3 Assemble the Stage
(1) Loosen the lock-screw①on the stage fully. (See
Fig. 4)
(2) From a rear area of the rounded hole center on the
base, carefully ring the two “V”buttons on the bottom
of the stage into the “V”rounded groove②, then screw
down the lock screw①.
Fig.3
Fig.2
Fig.1
Fig.4