OPTO-EDU A16.2615-L User manual

A16 . 26 1 5 - L
Inverted LED Fluorescent Microscope
Instruction Manual
To ensure the safety and obtain satisfactory performance, please study this instruction manual thoroughly be
fore start to use the instrument.

ATTENTIONS
1. Please clean sample-touching part after using.
Following the Fig.1 step to move the instrument, make sure the sample is
taken away. Use one hand holding ①position,the other hand holding②
position.
Once the sample is damaged, must take steps to avoid pollution.
If rising microscope’s height with other parts, must keep it in horizontal
position, avoid incline and sample slip.
Fig.1 Carrying Diagram
Fig.2 Replace Bulb
2. Before replacing the
lamp, first turn off
power switch at off
position O, and
unplug it in case of
electric shock and
burn.If microscope is
in use or after use,
the bulb and lamp
house must be
cooled off completely before proceeding, please see Fig.2 ,Loosening screw and
replace bad bulb with required bulb. The power supply must be cut off before bulb
replacement.
3. Microscope must be placed on stable and horizontal table.
4. Only use our power line, wrong power line can’t guarantee instrument safety and
performance.
5. The microscope ground terminal must be tightly connected with plug ground
terminal.
6. If unexpected situation happens ,please pull the plug out.
7. Disassembly only by the professionals. The microscope has been adjusted before
shipping, Unprofessional-person should not disassemble and remove any other
parts.
If you have any questions, please contact with manufacturer or local distributor.
8. Wide voltage input 100～240V,47～63Hz, if not in this range, it may cause damage
for equipment.

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9. Don’t open microscope lower plate when in use or else exposed electrical element
will lead to electric shock. Before replacing the lamp or fuse, please turn off power
switch and pull out the plug from the socket.
10. Do not use alcohol, gasoline, paper and other combustibles near the instrument, to
prevent fire ! !
MARK
MEANING
Surface will be hot, don’t touch it.
Please read manual instruction carefully at first. Misuse will lead to body
damage or instrument damage.
It is near the fuse socket , means be care of electric leakage.
Main switch “ ON ”
Main switch “ OFF ”
1）PREPARATION
a. Microscope is precise instrument, carefully operation, avoid collisions and shaky.
Using environment should not be in direct sunlight , high temperature or high
humidity and dusty, avoid violent shaky.
b. Tension for coarse focusing knob can adjustable.
c. Please leave enough space (10cm) for ventilation.
d. Please follow Fig.1 to carry microscope.
★Firstly, take away sample, filters and round stage parts avoid damage .
★If the microscope is leaning slightly, not in good position, rubber gasket may be fall
off.
2）MAINTENANCE AND STORE
a. Please use gauze with 70% ether and 30% alcohol mixed liquor to gently wipe the lens,
Alcohol and ether are inflammable material, please take them away from fire. Be
careful for turn on and off power . Please keep indoor air ventilation.
b. Please use soft fabric to clean other parts with neutral detergent besides glass parts.
c. If not in use, please put dust cover on microscope.
d. Please collect packing material for storage and carry after unpack carton.
Safety Mark

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CONTENTS
Attentions....................................................................................................................................... 1
1. Parts Name ......................................................................................................................................... 4
2. Observation Steps............................................................................................................................... 6
3. Operation............................................................................................................................................ 7
3.1 Main Body......................................................................................................................................... 7
3.1.1 Turn On Light Source.................................................................................................................. 7
3.1.2 Aperture Diaphragm.................................................................................................................. 7
3.1.3 Adjustment of Field Diaphragm and Lamp Filament................................................................. 7
3.1.4 Usage of Filter............................................................................................................................ 8
3.2 Focusing Unit .................................................................................................................................... 8
Adjustment of Coaxial Coarse Focusing Knob Tension ....................................................................... 8
3.3 Observation Tube.............................................................................................................................. 8
3.3.1 Adjustment Of Interpupilary Distance....................................................................................... 8
3.3.2 Adjustment Of Diopter .............................................................................................................. 9
3.3.3 Locking Eyepiece........................................................................................................................ 9
3.4 Stage ................................................................................................................................................. 9
3.4.1 Fixed Stage................................................................................................................................. 9
3.4.2 Mechanical Stage..................................................................................................................... 10
3.5 Condenser....................................................................................................................................... 10
3.6 Fluorescence Attachment............................................................................................................... 11
3.6.1 Mechanism of Fluorescence Production ................................................................................. 11
3.6.2 Parts Name .............................................................................................................................. 13
3.6.3 Fluorescence Observation Processes....................................................................................... 13
3.6.4 Mercury Lamp Power House ................................................................................................... 14
3.6.5 Replacement of Mercury Bulb................................................................................................. 15
3.6.6 Replacement of Mercury Lamp Fuse....................................................................................... 16
4. Trouble Shooting................................................................................................................................. 17
5. Installation.......................................................................................................................................... 18
5.1 Installation Diagram........................................................................................................................ 18
5.2 Installation Steps ............................................................................................................................ 19
5.2.1 Installation And Replacement of Bulb ....................................................................................... 19
5.2.2 Replacement of Fuse ................................................................................................................. 19
5.2.3 Connecting Power Line.............................................................................................................. 19
5.2.4 Installation Of Turnable Phase Contrast Condenser .................................................................. 20
5.2.5 Installation Of Photography System........................................................................................... 20
5.2.5.1 Video System (Optional) ................................................................................................... 20
5.2.5.2 DSLR Camera System(Optional) ........................................................................................ 21

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Fig.3 Main Parts Name
1）Eyepiece
2）Seidentopf Trinocular Head
3）Main Body
4）Adaptor for DSLR Camera
5）Coaxial Coarse and Fine Focusing Knob
6）Nosepiece
7）Attached Mechanical Stage
8）Fixed Stage
9）Turnable Phase Contrast Condenser
10）Lamphouse
1. Parts Name

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Fig.4 Control Parts
1）Lamp House
2）Turnable Phase Contrast Condenser
3）Liftable Knob for Turnable Phase
Contrast Condenser
4）Fixed Stage
5）Objective
6）Nosepiece
7）Surplus Water Plate
8）Main Body
9）Convertible handle for DSLR Camera
10）Infrared Sensors Indicator
11）Adaptor for DSLR Camera
12）Coarse & Fine Focusing Knobs
13）Fastening Screw for DSLR Camera
Adaptor
14）Culture Dish Holder
15) Trinocular Switch Lever
16) Seidentopf Trinocular Head
17) Aperture Diaphragm Lever
18) Eyepiece
19) Fastening Screw for Lamphouse
20) Field Diaphragm Lever
21) Adjustable Knob for lamp bulb
22) Attachable Mechanical Stage
23) Handle
24) Lamphouse Plug
25) Power Socket (with fuse holder)
26) Power Switch
27) Infrared Sensors Switch
28) Potentiometer Knob

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Please read chapter 6 about assembly steps.
Turn on main power source
Place sample
Put 10X objective in optical path
Focusing sample
Adjusting brightness
Adjusting
interpupilary
distance and diopter
2. Observation Steps
Adjusting lamp filament position
Adjusting field diaphragm
Putting objective in optical path and focusing
Adjusting aperture diaphragm
Turn filter into optical
path
Adjusting brightness
Observation

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3.1.1 Turn On Light Source (Fig.5)
a. Turn potentiometer knob②to the minimum, turn on power switch to①position I
(ON).
b. Rotating potentiometer knob②to increase and decrease brightness for good
illumination .
c. Push button○ 4 to open infrared sensors switch,if you leaving the Infrared sensors
window for 10 minutes , it will be off automatically. If someone closing to it ,the
bulb will turn to bright again.
Fig.5 Power Switch
3.1.2 Aperture Diaphragm (Fig.6)
Gently moving diaphragm lever ①to adjust aperture diaphragm for best image contrast .
Fig.6 Adjustment of Aperture Diaphragm and Placement Fig.7 Adjustment of Field Diaphragm and filament
Of filter
3.1.3 Adjustment of Field Diaphragm and Lamp Filament
a．Adjust field diaphragm lever○ 1to make diaphragm smaller until see the field. (Fig.7)
b．Adjust phase contrast condenser liftable knob○ 3(Fig.4)for for clear field image.
3. Operation
3.1 Main Body

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c．Adjust centring screw○ 3(Fig.6)to make field diaphragm coincide with field , fasten the
thumb screw○ 4(Fig.6),adjust field diaphragm to make image edge
d．Adjust lamphouse knobs○ 2○ 3○ 4(Fig.7)to make lamp filament image in the centre of
aperture diaphragm, keep left right image in symmetry.
3.1.4 Usage of Filter (Fig.6)
a．Take out the filter○ 5 .
b．Place the filer○ 5into the hole which above turnable phase contrast condenser.
Adjustment of Coaxial Coarse Focusing Knob Tension（Fig.8）
a. Coarse focusing knob tension can be adjustable ,hold adjustable ring ①and rotate it.
Anti-clockwise direction is decrease, clockwise direction is increase.
b. If stage is decreasing automatically, the sample is deviating from the focusing point,
means tension too low ,clockwise rotating adjustable ring ①to increase tension.
Fig.8 Tension Adjustment For Coarse Focusing Knob
Don’t rotate coarse and fine focusing knobs with reverse direction at the same time.
3.3.1 Adjustment of Interpupilary Distance
Please adjusting binocular tube to make left and right field coincide completely .
Indication point ●is interpupilary distance.
3.2 Focusing Unit
3.3 Observation Tube

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Fig.9 Adjustment of Interpupilary Distance Fig.10 Adjustment Of Diopter
3.3.2 Adjustment of Diopter（Fig.10）
Please turn adjusting ring scale 0to scale line at the first use.
a．With right eye to observation by right eyepiece, focusing the sample by coarse and fine
focusing knob .
b．With left eye to observation by left eyepiece, focusing the sample by coarse and fine
focusing knob .
3.3.3 Locking Eyepiece
Eyepiece can be tighten by using thumb screw
①(Fig. 11). If want to change eyepiece ,must
unscrew it at first.
Fig.11 Locking Eyepiece
3.4.1 Fixed Stage
a. Insert standard round plate ①in stage.
b. Install the clamps○ 2on the stage by screw joint.
Fig.12 Fixed Stage
3.4 Stage

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3.4.2 Mechanical Stage
a. Mechanical stage can be moved freely at X and Y direction.
b. Insert standard stage round plate○ 1 in stage.（also don’t insert round plate as needed）.
c. Install attached mechanical stage○ 2in stage with knurled screw○ 3 or hexagon socket cap
screws.
d. Put stage plate ○ 4 in stage as needed and fixed with clamps.
Fig. 13 Mechanical Stage
◎According to culture dish height to decide whether to disassemble the condenser.
a. Rotate liftable knob○ 2 to lower phase
contrast condenser.
b. Unscrew thumb screw①to disassemble phase
contrast condenser○ 5 .
c. Whe removing the condenser，please hold the
condenser with hand in case of falling and damage.
d. Knurled screw○ 3 is condenser centring screw，
condenser positioning screw○ 4 should be installed in
relevant hole.
Fig. 14 Condenser Installation
3.5 Condenser

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◎A16.2615 inverted biological microscope can configured with fluorescence
attachment（lamp house, power supply etc.）
3.6.1 Mechanism of Fluorescence Production
Ultraviolet(U), Violet(V), Blue(B), Green(G) transmittance characteristic curve.
UV ( Ultraviolet )Excitation V (Violet) Excitation
B( Blue Light )Excitation G (Green Light )Excitation
Fluorescence is a kind of photoluminescence in natural world.This kind of material will emit
more longer wavelength light than irradiation light wavelength ,if stop light, emitted light will
disappeared. The longer wavelength is fluorescent light. The substance which can launch the
fluorescent is called fluorescence material.
Stimulate the principle:
Now use blue-ray as example (B light, the wavelength of 490nm), when the light is emitted
from the DC mercury lamp, after condenser and stimulated color filter,get the
exciting light wavelength of 490 nm or so, then through color spectroscope reflection into the
objective, color spectroscope has the nature of selective reflection in a certain spectrum area.
It will completely reflect all light which is shorter than a certain wavelengths and all light which
is longer than a certain wavelengths of light will totally through it. According to this feature ,
color spectroscope can be designed corresponds to the excitation light beam splitter, it
reflects the light of wavelength under 490 nm, and the wavelength is more than 490 nm light
can through. So such a wavelength of 490 nm or so exciting light after color spectroscope,
3.6 Fluorescence Attachment

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greater than 490 nm light through color spectroscope to be removed, and the excitation under
490 nm light irradiation by fluorescence objective can converge on the fluorescent specimens,
specimen inspire greater than 490 nm wavelength of fluorescence(effective fluorescence
wavelength of 525nm) and fluorescence imaging by objective gathering, and through the
color spectroscope (with a small amount of under 490 nm exciting light is reflected back to
the light source and not through) and cut-off filter (cutoff wavelength less than 525 nm light),
pure fluorescent light beam for observation and photography.
Fluorescent Attachment Diagram
1) Mercury Lamp
2) Condenser
3) Excitation Filter
4) Specimen
5) Objective
6) Color Spectroscope
7) Filter for Resistance
8) Prisms Group For Inverting Light Path Direction
9) Eyepiece

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3.6.2 Parts Name
Fig. 15 Fluorescence Parts Name
1) Eyepiece 2) Trinocular Switch knob 3) Main Body 4) Adaptor for DSLR Camera
5) Fastening Screw for DSLR Camera Adaptor 6) Infrared Sensors Indicator
7)Coarse & Fine Focusing Knobs 8) Potentiometer Knob 9)Fluorescent Transforming Handle
10) Diaphragm Slider 11) Mechanical Stage Hand Knob 12) Power Switch
13) Infrared Sensors Switch 14) Nosepiece 15) Attached Mechanical Stage For 96-hole Plate
16) 100W Mercury Lamphouse 17) Stage Plate 18) Field Diaphragm Lever
19) 12V50W Halogen Lamphouse 20) Turnable Phase Contrast Condenser
21) Aperture Diaphragm Lever 22) UV Protecting Plate 23) Digital Camera 24) C-mount
3.6.3 Fluorescence Observation Processes
According to bright field observation methods to adjust microscope, take following steps to
make observation:
●Put mercury lamp power supply power plug into the power socket, please firstly check the
power supply voltage whether conform to the requirements of the instrument and supply
voltage.
●Open the mercury lamp power switch, mercury lamp power voltage fluctuation may not
be out of 100～240V ,otherwise affect the start of mercury lamp. It takes about 10 minutes
to reach stable state ,the maximum luminous efficiency.
●Put 10X objective in optical path. Put fluorescent specimen on the slide, with fixed clamp,
regulating stage vertical moving handle to make the objective in the optical path.
●Through push and pull the fluorescent transform handle, fluorescence excitation cube will

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be moved into light path .
●Adjusting coarse and fine focusing knob to get clear image.
●Adjusting the center of the low and high pressure mercury lamp.
After getting clear image by focusing , using condenser axial adjustment knob, mercury
lamp arc light can be seen in the eyepiece . If arc light regiment not in the center of the field,
by adjusting the vertical and horizontal adjustment knob of mercury lamp light box to make
the center of the arc light group in the field of view, adjusting the mirror, make the
brightness to the brightest. When arc light group in the center, adjusting condenser axial
adjustment knob to make arc light group into the whole field ,fluorescence observation can
be made.
Try to avoid frequent open mercury lamp power supply, it will reduce mercury lamp
working life. Once closed, mercury should be restart after five minutes later .
3.6.4 Mercury Lamp Power House
a) Main Parameter
Input Voltage : AC 100 ～240 V 47～63 Hz
Output Voltage: DC18V~ 40V Maximum
Stable Output Current: 3.6A ～ 4.9A
Start Stable time: 2 minutes
Fig.17 Adjustment For Mercury Lamp House
1) Condenser Adjusting Knob
2) Vertical Adjusting Knob for Mercury lamp
3) Horizontal Adjusting Knob for Mercury lamp
b) Use Of Power Supply
●Determine the input voltage is conform to the requirements of the instrument, can
open the main power switch, open the indicator lights on the rear panel is light,
indicating the power switch on.
●Gently press the trigger switch, power box above positive press switch in place can
let go, don't have to hold for a long time. If you don't can trigger mercury lamp, please
check the mercury lamp light boxes closed is complete.
●Mercury lamp light will be stable after 2 minutes commonly, lamp power
consumption between 90 W and 135 W.
●Mercury lamp service life is 100 hours or so, so use for a period of time after the
brightness will decrease.
●After replacing mercury lamp, power box timers need to reset ,pressing the reset
which on the front side of power box.
●Don't often press the power box timer reset button, also can't often time, according
to otherwise it will affect the service life.
●Don't no-load test output voltage, also don't open source box to adjust or modified,
in order to avoid damage to mercury lamp or power box.

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Fig.18 Front Side Fig.19 Back Side
1) Timer 2) Reset 3) Trigger Switch 4) Power Switch 5) Output Socket 6) Cooling Fan
7) Voltage Transfer Switch 8) Input Power Plug 9) Fuse Holder
3.6.5 Replacement of Mercury Bulb
Cut off the power supply, with inner
hexagon screwdriver to adjust the socket
head screw which in the rear of the mercury
lamp light box , then slowly remove the
mercury lamp light box upper portion, upside
down on top of the desktop. Unscrew two
mercury lamp set screw, remove the old
mercury lamp, change new mercury lamp,
then tighten the two fixed screw will recover
lamp holder, open screw tightening the
mercury lamp holder. Then according to the
mentioned methods for mercury lamp in front
of the center.
Fig.20 Replacement Of Mercury Lamp
●Don’t remove mercury lamp under the condition of electricity from inside the box!
Danger!
●Mercury lamp must be replaced after cooling.
●Note mercury lamp installation in negative direction, and the lamp glass shell on uneven
parts from the direction of the condenser.
●With gauze dipped in a little alcohol ether 4:6 mixture to wipe the lamp glass shell surface,
there is no dust, fingerprints, etc contamination tubes.

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3.6.6 Replacement of Mercury Lamp Fuse
Turn off the power, pull out the plug and open fuse box . Mercury power supply fuse：
Φ5×20mm，5.0A, 250V（The detail steps are as follows）.
1
.
2
.
3
.
Fig.21 Replacement Of Fuse Tube

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Trouble
Causation
Remedy
1. Optical System
a. Field incomplete or
illumination irregular.
Nosepiece not in right location.
Readjust nosepiece in right
position.
Filter slider not in right place.
Readjust filter slider
Phase Contrast Slider aren’t moved out of optical path.
Moving out the slider.
b. Dirt or dust in objective field.
Lens of collector is dirty.
Thoroughly clean.
Sample is dirty.
Eyepiece is dirty.
c. Dazzling image
Aperture diaphragm isn’t wide enough.
Readjust it.
d. Bad Image quality, for
example, not sharp ,low
picture contrast, image detail
not clear.
Objective isn’t in right position.
Rotate nosepiece to locating
position.
Objective front lens is dirty.
Thoroughly clean .
Culture dish bottom is thick.
Use normal size culture dish.
Culture dish optical performance isn’t good.
Use standard culture dish.
Sample is dirty.
Thoroughly clean
e. Partial image isn’t clear or
unsteady .
Objective isn’t in right position.
Rotate nosepiece to locating
position.
Sample isn’t placed at right stage place.
Correctly placing sample and
fixer it with holder.
f. The effect of phase contrast
observation image is poor.
Annular spot don’t focus with dish holder.
Readjust it.
Culture dish bottom isn’t flat.
Use standard culture dish.
2. Mechanical Focusing Unit
a) Coarse focusing knob is too
tight.
Tension too big.
Loosing tension adjusting ring，
reset again.
b) Nosepiece glide down
automatically.
Tension too small.
Tightening tension adjusting ring,
reset again.
3. Binocular Observation Tube
Field of Binocular observation
tube isn’t inconsistent.
Wrong interpupillary distance.
Correctly set again.
Binocular diopter is incorrect.
Left eye and right eye with different eyepiece.
Change one eyepiece, to make it
same as another eyepiece.
4. Nosepiece
When using high power objective,
it will touch the sample.
Improper sample.
Use right sample.
Culture dish bottom is too thick.
Use standard dish.
5. Power System
a) Bulb don’t work.
No bulb.
Install bulb.
Bulb or fuse is broken.
Change new bulb or fuse.
Don’t plug in power.
Plug in power safely.
b) Bulb is easy broken.
Don’t use specified specification bulb.
Use specified specification bulb.
No bulb.
Install bulb.
加装机械载物台
4. Trouble Shooting

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Fig. 21
○
a Centring Telescope ○ b Eyepiece ○ c C-mount ○ d Filters
○
e Turnable Phase Contrast Condenser ○ f Halogen Lamphouse ○ g Clamps
○
h Stage Round plate ○ i○ j○ k Culture Dish Holder ○ l Blood Cell Counter Holder
○
m Attached Mechanical Stage For 96-hole Plate ○ n Surplus Water Plate
○
0 LWD Infinity Plan Phase Contrast Objectives ○ p LWD Infinity Plan Objectives
○ q Mainbody ○ r Adaptor for DSLR Camera ○ s DSLR Camera
5. Installation
5.1 Installation Diagram

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5.2.1 Installation And Replacement of Bulb (Fig.2 ,Fig.4)
WARNING: Cut off the power line plug before replace bulb.
a. Completely loosening screw on the top of the lamp house, pulling out lamp shade.
b. Loosening bulb pins locking screw , using glove or gauze to hold the bulb and insert fully
into the socket .
c. Install the lamp again.
◎Bulb : 6V 30W Halogen
5.2.2 Replacement of Fuse
WARNING：Turn off the power plug before replacement.
Fuse box is installed below the power socket, fuse rating: Φ5×20mm , 5A/250V.
Fig. 22 Replacement Of Fuse
5.2.3 Connecting Power Line
a.Don’t curve or twine power line .
b. Make sure the main switch at off mark
Owhen connecting power lines.
c. Must use three-phase plug which can
connect ground electrode, Otherwise
can’t use the microscope. Fig. 23 Power Line Connection
If power line is close to lamp house or relevant device, it will melt and cause electric leakage. So
don’t close to lamp house.
5.2 Installation Steps