OPTO-EDU A15.1091 User manual

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A15.1091
Polarizing Microscope, Semi-APO,
Transmit & Reflect Light
Instruction Manual
This manual is applicable to A15.1091 laboratory biological microscopes. In order to ensure safety,
perform optimally with the instrument, and make you fully familiar with the use of this microscope, we
recommend that you read the manual thoroughly and carefully before operating the microscope.

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Contents A15.1091
Before Use ................................................................................................................................ 4
1.Name of each part ................................................................................................................. 6
1.Name of each part................................................................................................................... 7
1. Name of each part.................................................................................................................. 8
2.Overview of each institution .................................................................................................. 9
3. Adjustment and operation .................................................................................................. 15
3-1 Turn on the power (Figure 1)........................................................................................ 15
3-2 Adjust light intensity and switch up and down light source (Figure 2) ........................ 15
3-3 Light socket (Figure 3).................................................................................................. 15
3-4 Specimen placement (figure 4）................................................................................... 16
3-5 Adjusting the pitch (figure 5)........................................................................................ 16
3-6 3rd-gear optical circuit switch (Figure 6)...................................................................... 16
3-7 Focusing (Figure 6 -8）................................................................................................ 17
3-8 Focusing handwheel slacking adjustment (Figure 8).................................................... 17
3-9 Focus upper limit (Figure 9) ........................................................................................ 17
3-10 Swing-out condenser adjustment (Figure10 , 11）..................................................... 18
3-11 Use of color filters (figure 12）.................................................................................. 19
3-12 The use of extended function board (Figure 14)......................................................... 19
3-13 ECO function key (Figure 15).................................................................................... 19
3-14 Fuse replacement(Figure 16)....................................................................................... 20
3-15 “ECO"The device(Figure 18)...................................................................................... 20
3-16 The use of Bertrand lens(Figure 19) ........................................................................... 21
3-17 Bertrand lens focusing(Figure 19) .............................................................................. 21
3-18 Use optical path selection(Figure 20).......................................................................... 21
3-19 Centering the field diaphragm (Figure20 , 21)............................................................ 21
3-20 Use aperture diaphragm(Figure 23) ............................................................................ 22
3-21 Use the polarizer and the analyze (Figure 24)............................................................. 23
3-22 Adjusting the objective light axis and stage center coincide(Figure26 , 27）............ 23
4．Install................................................................................................................................. 24
4-1 Installation illustration .................................................................................................. 24

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4-2 步骤............................................................................................................................... 25
4-2-1 Installation of mechanical carrier bays（Figure 1 , 2）........................................ 25
4-2-2 Install the polarizing platform (figure 3, 4）......................................................... 25
4-2-3Install the polarizing moving ruler (Figure 5)......................................................... 26
4-2-4 Installation converter (Figure 6) ............................................................................ 26
4-2-5Install metallographic device (Figure 7)................................................................. 26
4-2-6 Install Bertrand lens set (Figure 8)......................................................................... 27
4-2-7Install observation head and phot-ography accessories /0.5X C interface (Figure 9)
......................................................................................................................................... 27
4-2-8Installation eyepiece (Figure 10) ............................................................................ 28
4-2-9 Install objective lens (figure 11）.......................................................................... 28
4-2-10 Install swing-out condense (Figure 12）............................................................. 28
4-2-11Replace the camera interface (optional) (Figure 13 and 14）.............................. 29
4-2-12 Install the light box (figure.15）......................................................................... 29
4-2-13 Installation analyzers (Figure 16)........................................................................ 29
5. Technical specifications ...................................................................................................... 30
6．Troubleshooting guide....................................................................................................... 31

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Know Before Use A15.1091
Ⅰ.Safety signs
The following signs are on the microscope. Find out what these symbols mean and always use the
microscope in the safest way.
Ⅱ.Safety precautions
1. Care should be taken when opening the box, to prevent the lens glass stick to fingerprints, sweat
stains and other impact observation, to prevent the lens and other accessories fall damage.
2. Avoid placing the microscope in areas with direct sunlight, high or high humidity, dust, and
vulnerability to strong vibrations, ensuring that the stage is flat, horizontal and strong enough.
3. When a moving microscope is required, one hand hooks into the slot above the rack and one
hand holds the front bottom of the frame.
4. If a bacterial solution or water is splashed on a load station, objective or observation tube,
immediately pull out the power cord and dry the solution or water to ensure that the microscope is
dry. Otherwise, it is possible to damage the instrument.
5. To avoid blocking natural convection air for cooling, make sure that the left, right, top, and
back of the microscope are at least 10 cm from walls and other objects.
6. Ground the unit to avoid lightning strikes.
7. To ensure safety, make sure that the main switch is in an"O"(off) state and that the power is
switched off while waiting for the bulb and light chamber to cool down completely before
replacing the halogen lamp or fuse. Input voltage check: The input voltage indicated on the back
of the microscope is consistent with the supply voltage, otherwise it will cause serious damage to
the microscope.
8. Use the special wires provided by the Company.
Sign
Significance
Indicates that the main power switch is on.
Indicates that the main power switch is off.
Indicates that ground is here.
/
Indicates on/down illumination.
Indicates that the upper/ under light source illumination when the button is
pressed/not pressed.
Represents the trash can.
F.S represents diaphragm, represents the opening direction of the
diaphragm, represents the narrowing direction of the diaphragm.
Indicates that the person walks the light off switch.
Represents a photo symbol.
Indicates use a wrench here.
Indicates the light strong small direction, the small head is the light
weakening, the big head is the light to become stronger.
Indicates CE certification.

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A15.1091
Ⅲ.Maintenance and maintenance
1. All lenses are tuned and do not disassemble themselves.
2. Objective converter and coarse micro-focusing mechanism, the structure is precise, please do
not easily disassemble.
3. The instrument should be kept clean, dust removed every day, clean should pay special
attention not to contaminate optics, objective lenses every other month to ask professionals to
clean.
4. Smudges on the lens, such as fingerprints and grease, can be wiped with a small amount of
ether (70%) and alcohol (30%) mixed solution on the lens paper.
Solvents such as ether and alcohol are highly flammable, do not carry out a variety of
electrical equipment power switch operation, at the same time can not approach the
open fire, please ensure indoor ventilation.
5. Do not use organic solvents to wipe non-optics of the microscope, if cleaning, use a neutral
detergent.
6. When using, if the microscope is wet with liquid, cut off the power supply immediately and
dry it.
7. Never disassemble any part of the microscope, which can affect the function of the
microscope or reduce the performance of the microscope.
8. The instrument should be placed in a cool, dry place, when the microscope is not used, the
use of a dust cover. Be sure to wait for the light chamber to cool down before the cover is on.
9. The environmental requirements for the use of microscopes:
a）Indoor use；
b）Ambient temperature range：10℃~35℃；
c）Maximum relative humidity: 80% when the temperature reaches 31℃,70% When the
temperature is reduced linearly to 34℃,60% the temperature reaches 37℃,5 0%When the
temperature reaches 40℃.
10. Microscope storage and transport environment requirements:
a）Ambient temperature range：-40℃~+70℃
b) Relative humidity range:10% to 100%.
c) Air pressure range:500hpa-1060hp
Warning:
Failure to operate the microscope in the manner specified in this manual may
compromise the safety of the user. In addition, the microscope may be damaged. The
microscope should always be operated in accordance with this instruction.
◎This microscope does not cause radiation, electromagnetic interference, etc. To
the surrounding environment, in accordance with UL certification standards.
This manual practically the following symbols label prominent text:
The indication that the warning stake in this instruction manual will result in
personal injury to the operator and/or damage to the instrument (including objects
near the instrument).
★indicates that failure to follow this instruction will cause damage to the instrument.
S s indicates a comment (to operate and maintain at easy.

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Eyepiece
1.Name of each part A15.1091
Three-eye head
Burgundy
mirror device.
Converter
Object
ive.
Polarizing
platform.
Spotlight.
Host.
A15.1091
Polarizing
bay.

7
A15.1091-TRF
Eyepiece
1.Name of each part A15.1091
Three-eye
tilted head
Burgundy
mirror
device.
metallographic
device
phase device
Converter
.
Objec
tive.
Polarizing
platform.
Spotlight.
Host.
Polarizing
bay.

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Objective
Polarizing
platform
Eyepiece
1. Name of each part A15.1091
Three-eye
tilted head
Burgundy
mirror device.
metallographic
device
Converter
Host
A15.1091-RF

9
2.Overview of each institution A15.1091
1
2
3
4
5
6
7
8
9
10
11
12
13
1. Filter switch bar
2. Rough /fine-tuning lift
handwheel
3. Focus the upper bit lock the
handwheel
4. Light up and down the toggle
button
5. Brightness adjustment knob
6. “ECO” Infrared sensing device
7. The height adjustment of the
spotlight handwheel
7. Polarizer
8. Condenser diaphragm
adjusting ring
9. Adjusting screw
10. Platform cursor
11. Extended function plug
fastening screws
12. Expansion function board
13. Burgundy mirror turntable
14

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A15.1091
1. Observe the light path switch
2. Analyzer
3. Condenser fastening screw
4. The height of the spotlight adjusts
the handwheel.
5. Taking pictures
6. The field diaphragm light size
adjustment knob.
7. Rough /fine-tuning handwheel.
8. Rough tension adjustment
handwheel
9. Light chamber fastening screws
10. Ground screw
11. Light room socket
12. Light room plug
13. Universal Wrench
1
2
3
4
5
6
7
8
9
10
11
12
13

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4
11
10
9
8
7
14
A15.1091-TRF
A15.1091
1
2
5
3
6
12
15
13
16
1. Filter switch bar
2. Rough /fine-tuning lift handwheel
3. Focus the upper bit lock the handwheel
4. Light up and down the toggle button
5. Brightness adjustment knob
6. “ECO” Infrared sensing device
7. The height adjustment of the spotlight
handwheel
8. Bias device
9. Condenser diaphragm adjusting ring
9.
10. Adjusting screw
11. Platform cursor
12. Extended function board fastening
screws
13. Extended function board
14. Labels
15. Light and dark field switch turning
wheel
16. Burgundy Mirror Turntable
17. ND filter slot
17

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13
15
1. Observe the light road switch
2. Analyzers
3. The analyzers slot
4. Light gate switch button
5. Condenser fastening screw
6. The height of the spotlight adjusts the
handwheel
7. Taking pictures
8. The field diaphragm light size
adjustment knob
9. Rough fine-tuning handwheel
10. Rough lying loosens the handwheel
A15.1091-TRF
A15.1091
1
2
3
4
5
6
7
8
9
10
11
12
14
16
17
18
19
11. Light chamber fastening screws
12. Ground screw
13. Light room socket
14. Light room plug
15. Universal wrench
16. The upper illumination aperture
diaphragm adjusts lever
17. Diaphragm adjusting screw
18. The upper illumination field
diaphragm adjustment lever
19. Polarizer slot

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1. Rough /fine-tuning lift handwheel
2. Focus the upper gear bit Lock the
handwheel
3. Brightness adjustment knob
4. “ECO" Infrared sensing device
5. Adjusting screw
6. Polarizer
A15.1091-RF
A15.1091
1
2
3
4
5
6
7
8
9
10
11
12
7. Extended function board fastening
screws
8. Extended function board
9. Labels
10. Light / dark field switch
11. Burgundy mirror turntable
12. ND filter slot

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1
3
8
1. Observe the light road switch
2. analyzers
3. analyzer slot
4. Light gate switch button
5. Rough/ fine-tuning handwheel
6. Rough tension adjustment
handwheel
7. Ground screw
6
7
5
A15.1091-RF
A15.1091
2
4
10
11
12
9
8. Light room socket
9. Universal Wrench
10. 16.The upper illumination aperture
diaphragm adjusts lever
11. Diaphragm adjusting screw
12. The upper illumination field
diaphragm adjustment lever
13. The polarizers slot
13

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Switch levers
from up and down
the light source.
3.Adjustment and operation A15.1091
Figure 1
3-1 Turn on the power (Figure 1)
Turn on the power and dial the main switch (as
shown) on the back of the microscope body to
the "-" (on) state.
3-2 Adjust light intensity and switch up and
down light source (Figure 2)
1. As the direction of the figure 2 arrow rotates, the
light source weakens and the light source is
enhanced in reverse.
2. As in Figure 2,when the upper and lower light
toggle button is pushed in the state of the upper
light source state, as shown in the figure
;when the upper and lower light
toggle button is pushed in the state of the lower
light source state , as shown in the figure
.
3-3 Light socket (Figure 3)
The 3 lamp source socket and the plug have red
dots on the figure 3, and before the plug is
inserted into the socket, the direction should be
adjusted until the red dot aligns in the same
direction.
Note that the silk screen on the left and right
sockets corresponds to the upper and lower light
switch buttons.As shown in the figure:
for the upper and lower light switch button push-
in state corresponding to the upper and lower
light source switch ingest state, for the lower
light circuit socket, with the upper and
lower light source switch button ejection status.
If you find that the upper and lower light
switching levers are not in the same state as the
illustration, check that the upper and lower light
plugs are inserted into the appropriate socket as
shown.
Dim the
handwheel
.
Red
dot.
Figure 2
Figure 3

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A15.1091
3-4 Specimen placement (figure 4）
Slowly push the cover slide up into the moving
ruler and gently clamp the carrier slide.
Move the sample to the desired position by ①on
The Rotating Figure 4.
Be careful when replacing objective lenses. The
objective mirror may touch the specimen when
the objective mirror needs to be replaced after
viewing the specimen with a objective at a short
working distance.
◎Before observation, it is necessary to adjust the
light axis and the core colocation of the rotating
carrier. Refer to sections 3-22 for adjustment.
3-5Adjusting the pitch (figure 5)
The pitch range is 47mm to 78mm. When looking
at the eyes, hold the left and right prism seat
around the axis rotation, to adjust the pitch, until
the eye observation, left and right viewing the
occasion two as one, observe the comfort so far.
3-6 3rd-gear optical circuit switch (Figure 6)
Select the desired light path and light-strength ratio
as shown in Figure 6.
Icon.
Operation.
Eye:Cam
era (%)
The push lever
is fully pushed
in.
100：0
Push lever put in
the middle.
20：80
The push lever
is completely
pulled out.
0：100
Figure 6
Figure 4
Figure 5
①
Light path
switching
signs.
Push lever.

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A15.1091
Figure 7
Figure 8
3-7 Focusing (Figure 6 -8）
1. When the camera device is not used.
Push the light-way switching push lever 6 (shown in
Figure 6) completely for binocular observation. With 10
×objective focus, in order to prevent specimens and
objective spades, the mechanical carrier table should first
rise, so that the specimen and objective close, and then
slowly reverse the rotation of the rough hand wheel, so
that the specimen dropped, while searching for images in
the 10×eyepiece, and finally with fine-tuning the focus
wheel finely focus to the clarity. At this point, switch to
another multiplier objective lens, can achieve focus
without damaging the specimen
2. When a camera device is required.
Push the light-way switching push lever (shown in Figure
6) to the end, double-eye observation, image clearly, and
then pull it out, which can be observed through the
camera system.
The micro-hand wheels on both sides are easy to
remove, and the hand wheels are absorbed with strong
magnetic suction. Simply, as shown by the arrow in
the figure, hold down one end of the wheel to make the
other up, and then gently pan from the end of the
upturn. Assembly also requires only the center of the
handwheel alignment in and in place. This feature
makes the two spinners easy to swap from left to right
to avoid the fine-tuning wheels touching the platform
handle.
3-8 Focusing handwheel slacking adjustment
(Figure 8)
The focusing loose handwheel 8 rotates in the direction
shown by the Figure 8 arrow, and as the more the
rotation, the tighter the focusing handwheel. The
opposite is looser.
3-9 Focus upper limit (Figure 9)
In practical use, if you want to limit the height of the
platform, that is, the upper limit position of focus, if you
want to limit the lock, you can simply turn the locked
handwheel in the direction of the Figure 8 arrow at the
corresponding upper position.
Tighten the handwheel.
Figure 9

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A15.1091
Figure 10
Figure 11
5. Turn the two spotlight-to-center screws③with a universal adjustment wrench to move th -e fie
ld of view to the center of the field of view.
6. Gradually turn on the field of view, if the field-of-view light image is in the center an -d conne
cted to the field of view, then the spotlight is correctly paired.
7. In practice, slightly increase the field of view, so that its image just cuts off the field of view.
8. Adjustment of aperture light ④
Aperture light is designed for the adjustment of numerical aperture, not brightness adjustment.
In general, a good image with sufficient contrast is obtained when the aperture light is turned on
to 70 to 80%percent of the objective lens. To observe the aperture image, it is advisable to go
under the eyepiece and look down from the eyepiece barrel to the object lens.
9. The adjustment of the size of the field of view light
During operation, turn the field of view light turning circle ②, reduce the field of view, observe
the field of view, if the light image blur, can turn the spotlight eyepiece focusing handwheel, lift
the spotlight mirror carrier, so that the observation field of view light image clear, and then turn
the field of view light, so that it is just adjusted to the eyepiece field, to reduce the complex,
improve the quality of the image.
3-10 Swing-out condenser adjustment
(Figure10 , 11）
⚫The center of the spotlight should be concentric
with the objective optical axis, the product has
been adjusted at the factory, the user does not
have to adjust itself.
⚫The highest position of the spotlight mirror, has
been adjusted at the factory, the user does not
have to adjust itself.
⚫Turn the focus handwheel ①to move the
spotlight mirror up and down, when using a
high-gloss objective, the spotlight mirror rises,
and when using a low-multiplier objective, the
spotlight mirror can be lowered.
Spotlight pair:
1. Turn the spotlight to focus the handwheel①and
raise the spotlight to the highest position.
2. Focus the sample with a 10×objective lens.
3. Rotate the field-of-view light ring②to reduce
the field-of-view photo pal image so that it is
visible in the field of view.
4. Turn the spotlight to focus the handwheel①to
focus the field-of-view photobook image.
①
④
③
②

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A15.1091
Figure 12
3-11 Use of color filters (figure 12）
As shown in Figure 12, when the push rod is pushed in, it is the
color filter usage state, and when the putter is pushed out, it is
the filter empty state .LBD is a color filter with a color
temperature filter, ND25 is a color filter with a light
transmission rate of 25%, and ND6 is a color filter with a light
transmission rate of 6 %.
When transmitting illumination, insert the filter module as
needed at the filter slot position ①in Figure 13.The first
positioning is empty, pushing until the second position, the color
filter enters the light path.
Color filter.
Application.
Color temperature
Converts illuminated light into
daylight for general observation
and color photography
Green color filter
Increased contrast when viewed in
black and white For black-and-
white photography
Yellow color filter
Increases contrast when viewed on
semiconductor sheets
The sand sheet
both light, but reduces light
strength
ND6
Adjust light strength (transmission
rate 6%)
ND25
Adjust light strength (pass rate
25%)
3-12 The use of extended function board (Figure 14)
As figure 14,a slot for the expansion panel is reserved on the
converter, and the microscope mirror has been inserted into the
slot with a normal light and dark field plate. To use other
expansion function boards, simply spin the serial number ②out
of release, pull out the standard light and dark field board, and
then insert the expansion function board into the slot and tighten
the screws of the serial number ②.
3-13 ECO function key (Figure 15)
After leaving the microscope for 15 minutes, the light will
automatically turn off and the indicator will flash once every three
seconds. When you come back and see the indicator light flashing,
press ECO to re-activate the light and turn it on.
ECO function
key .
push rod .
Figure 13
①
Figure 14
Figure 15

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A15.1091
3-14 Fuse replacement(Figure 16)
The drawer of the fuse box is first6 opened
with a universal adjustment wrench, as shown
in Figure 16. As shown in Figure 17, gently top
the fuse from the bottom to the top of the
square hole under the fuse drawer with a
universal adjustment wrench.
The middle of the fuse is a thin glass, and
carefully and gently operate when opening
the fuse box drawer and ejecting the fuse.
3-15 “ECO"The device(Figure 18)
As shown in Figure 18, the device can sense
objects within 1 meter of its front.
When a person is observed, within 1 meter of
its front, the unit appears as a red light, at
which point the microscope light source does
not go out.
The red light flashes when a person walks
away and there is no other object within 1
meter of his or her front. The light source
automatically goes out after this state lasts for
a period of time. The duration defaults to 15
minutes and can be set according to user
requirements in the companion software
settings.
When the light is not on, the microscope light
source is out. Press the “ECO” function key
to restore the light on state.
Figure 16
Figure 17
Figure 18