OPTO-EDU A11.1009 Series User manual

A11.1009
Series Student Microscope
Instruction Manual
Please read this manual carefully before use microscope!

Eyepiece
Objective
Monocular Head
Coarse Focusing
Knob
Fine Focusi
ng
Knob
Brightness
Adjustment
Knob
Main
Switch
Stage
Condenser
Collector
Eyepiece
Objective
Stage
Condenser
Collector
Sliding
Binocular
Head
Coarse
Focusing
Knob
Fine
Focusing
Knob
Brightness
Adjustment
Knob
Main
Switch

1 Appliance
N-10 biological microscope is widely used in medical and hygienic
establishments for conventional microscopic examination, general biologic,
pathologic and bacteriological studies, clinical investigations and
classroom demonstrations. Designed in a very modern way what can take
convenient and safe to your operations.
2 Specification
2.1 Mechanical tube length：160mm
2.2 Objective：
Magnification Numerical
Aperture
（NA）
Focus
Distance
(mm)
Working
Distance
(mm)
Remark
4X 0.10 31.05 18
10X 0.25 17.13 7
40X 0.65 4.65 0.53
100X 1.25 2.906 0.13
2.3 Eyepiece
TYPE Magnification Focus
Distance(mm)
Field of view
(mm)
Plan Eyepiece 10X 25 Ø18
2.4Total Magnification
Eyepiece 10X 10X 10X 10X
Objective 4X 10X 40X 100X
Total 40X 100X 400X 1000X
2.5 Conjugate Distance: 195mm
2.6 Stage：
N-10D、E: Double Layers Mechanical Stage 116mmX125mm
N-10A、B: Attachable Mechanical Stage 110mmX120mm
N-10C: Plain Stage with Slide Clips 110mmX120mm
2.7 Coarse Focusing range：10mm
2.8 Condenser：
N-10D、E: Abbe NA=1.2with Iris Diaphragm,Rack and Pinion
Adjustment
N-10A : Single Lens NA 0.65 with Disc Diaphragm
N-10B : Single Lens NA 0.65 with Iris Diaphragm
N-10C : Abbe NA=1.2with Iris Diaphragm,Spiral Adjustment
2.9 Diameter of Color Filter：Ø32mm
2.11 Illumination ：
N-10A、B、C、D:Incandescent Lamp Illumination 15W,
Brightness Adjuseable
N-10E: LED Lamp Illumination 1W, Brightness Adjuseable
3 Outfits
Component
Name
Specification
N-10A N-10B N-10C N-10D N-10E
Viewing
Head
Monocular Head,
inclined at 45° ● ●
● ●
Sliding Binocular
Head,Inclined at 45°
●
Eyepiece
Wide Field Eyepiece
WF10X ● ●
● ● ●
Eyepiece WF5X,P16X ○ ○
○ ○ ○
Eyepiece WF10X with
Pointer ○ ○
○ ○ ○
Objective
(Achromatic)
4X,10X ,40X(S) ● ●
● ● ●
20X,60X ○ ○
○ ○ ○
100X ○ ○
○ ○ ●
Condenser Single Lens NA 0.65 ●

with Disc Diaphragm
Single Lens NA 0.65
with Iris Diaphragm
●
Abbe NA=1.2with Iris
Diaphragm,Spiral
Adjustment
●
Abbe NA=1.2with Iris
Diaphragm,Rack and
Pinion Adjustment
● ●
Nosepiece Triple Nosepiece ● ●
● ●
Quadruple Nosepiece ●
Stage
Plain Stage with Slide
Clips 110×120mm
● ●
Attachable Mechanical
Stage
110
×
120mm / 60
×
30mm
●
Double Layers
Mechanical Stage
125
×
115mm / 70
×
20mm
● ●
Illumination
Incandescent Lamp 15W
Brightness Adjustment ● ●
● ●
LED Lamp 1W
Brightness Adjustment ●
Mirror ○ ○
○ ○ ○
Focusingg
Systerm
Caaxial Coarse and Fine
Adjustment,Moving
Range 10mm
● ● ● ● ●
Filter Green ●
● ●
Blue ●
● ●
Note: ● Standard Outfit, ○ Optional
4 Configuration
a) Eyepiece, Objective and Nosepiece
The monocular microscope has wide field eyepiece 10X and
achromatic objective 4X, 10X, 40X and 100X. The nosepiece
is small structured, and located stably and exactly. It has a
large and sharp image, with 360º rotatable monocular or
sliding binocular head.
b) Bend arm and Coarse and fine focusing knob
The curved arm use C-shaped ,has sprightly lines.Also,
with upper focus stop.the coarse focusing adjustment
adopts dovetail guide which make focusing adjustment
smooth and comfortable.
c) Base：
The base is designed to the instrument, which having a
streamlined shaped circular,and it harmonized the aim’s
shape slinky，Electrical components and lamp built inside it
as the illuminator.
d) Stage：
Fixed stage or moveable stage
e) Illumination
The system consists of illuminator which contains collector /
mirror, iris aperture diaphragm and condenser. And the
condenser can be lift up and down.
5 Assembly
a) Working environment requirement：
1) Room temperature: 0-40℃.
Maximum comparatively humidity: 85%.
2） High temperature will result in mildew, dew and even
ruinous instrument.

3) Avoid from the dust room. When it is not in use,
please cover the microscope with dust cover.
4） Please place the microscope in a stable situation
without any surging.
b) Check the Input voltage: be sure the power supply voltage
is accordant with the nominal input voltage which is signed
outside the microscope, or it will bring a serious damage to the
microscope.
c) Lamp
1. The lamp has been well adjusted and checked before
the microscope leaves factory.
2 . The lamp housing parts become extremely hot during
operation. Take care not to burn yourself.
3. Lamp replacement：The lamp has its standard service
life. When it has expired, a lamp replacement is
necessary.set the main switch to off state, disconnect
the power cord from the wall outlet, then allow the old
bulb to cool before replacing the bulb with a new of the
designated type .Open the lamp holder on the bottom
of microscope to do the replacement, the surface of
the new lamp bulb should be clean and free of
fingerprint or dirt, which will decrease the brightness or
even explode the bulb .
Note: Must not missing the grounded screw
Donot touch the lamp`s glass part with your
hands,when sitting shoud take gloves or cover the
lamp with protector. Fingerprint or dirt on the surface of
the lamp should be cleaned with the clean cloth with
dipping alcohol,which will decrease the brightness or
even explode the bulb.
d) Eyepiece：
Please insert the eyepieces into the eyepiece
tubes.(There is small screw in the eyepiece ,which can
make the eyepiece can not be pull out arbitrarily when
be screwed down.
e) Objective：
Lower the stage to its lower limit, then screw down the
objectives into the nosepiece (from low to high).
f) Condenser：
he condenser has been adjusted well before delivery.
g) Collector：
The collector has been adjusted well before delivery.
Frame of the collector`s pedestal is screw thread can
taken by contrarotate
Note：It cannot be taken down when the outfit is LED.
h) Color filter：
Lay the filter into the iris diaphragm bracket under the
condenser if necessary.
6 Operation
1、 Insert the eyepiece into the inclined eyepiece tube, then
rotate the objectives in sequence, according to their
magnifications, into the threaded holes of the nosepiece.
Place the specimen in the center of the field of view, First
use low power objective to find the specimen image and
then observe it with high power objective, at the same
time, adjust fine focusing knob until the image is clear.
When using 100X oil-immersion objective, the space
between the front of objective and specimen surface
should be full of cedar oil. Drops of cedar oil must be

strictly free from air bubble, and the objective should be
cleaned immediately after using, otherwise it may
solidify and make cleaning difficult.
2、 In order to get a sharp and clear image, you must adjust
the illumination system. When using objectives of
different magnification, please adjust the iris diaphragm
of the condenser to bring its numerical aperture and
objective’s numerical aperture into coincidence. Generally
speaking,the rate of objective increase， the location of the
conderser need to move up..Aperture is design for adjust
NA and not for adjust brightness.Usually,aperture is open to
70~80% of the objective`s exit pupil,until can see clearly
image in the view.
3、 When to watch the same type of specimens ,can adjust limit
screw to proper position and lock it up with nut.Then directly
make coarse focusing knob up to the position where the frist
speciment can focusing clearly.That can simplify the
operation and raduce the working intensity.
Note: The same type of specimen refer to the thickness of
the slice 、the coverslip and the carryslip are uniform.
7 Maintence and storage
1、When you open the carton, please be careful not to make
the lens dropping
2、All the lens has been adjusted by manufacturer already,
please do not disassemble by yourself.
3、Nosepiece, coarse and fine focus is installed precisely,
please do not disassemble by yourself.
4、You should make the instrument clean, and often wipe the
dusts.
5、Place the instrument in a shady, cool and dry place, when
finishing the operation, always use the dust cover for
protection.
8 Troubleshooting Guide
If problems occur during use, please review the following list
and take remedial action as needed. If you cannot solve the
problem after checking the entire list, please contact the
service department for assistance.
1、Optical Part
Problem Cause Solution
the edge of the field
of view has shadow
or the brightness is
uneven
The nosepiece is not
in he located
position(objective is
not in the center of
the optical path)
Turn to the right
position （Turn
objective to the
optical light
center)）
The image of the
filament is not in the
center
Center it
The surface of the
lens is moldy or has
contaminant (
including condenser,
objective, eyepiece
and collector)
Clean it up

Find dust and stain
in the field of view
There are stains on
the lens (include
condenser,objective,
eyepiece and
collector)
Clean it up
There are stains on
the specimen
Clean it up
The position of the
condenser is too low
Loosen the
condenser’s
locking bolt adjust
the
condenser to the
right position, then
screw down.
the image is
defocus(low
resolution/contrast)
There is no
coverslip on the
specimen
add coverslip
The coverslip is too
thick or too thin
Use the standard
coverslip
The specimen is
placed inversely
Reverse it back
There was oil on the
dry objective(easily
happened in 40X
objective)
Clean it up
There are stains on
the lens （including
condenser,
objective, eyepiece
and collector）
Clean it up
The oil objective
didn’t immerse oil
Use immerse oil
There was bubbles
in the oil
Eliminate the
bubbles
Use a unsuitable oil Change to the
specified one
The size of the
aperture diaphragm
is too big
Minify it
There are stains on
the incident lens of
the binocular tube
Clean it up
The size of the
aperture diaphragm
is too small
Open it up
The position of the
condenser is too low
Adjust the position
One side of the
image is dark
The condenser is
not in the center of
the field of view\the
condenser inclines
Install the
condenser again
and adjust the
center carefully by
the centering bolt
The nosepiece is
not in the right
position
Turning it until it
reach the “clicked”
position
The specimen is
floating
Fix it
The image shifts
during focusing
The specimen slips
on the stage
Fix it

The nosepiece is
not in the right
position
Turn it to the
“ clicked ”position
The image is a little
yellow
Not use the blue
color filter
Use the blue filter
The brightness is
not enough
The size of the
aperture diaphragm
is too small
Adjust again
The position of the
condenser is too low
Adjust the position
There are stains on
the lens （include
condenser,
objective, eyepiece
and collector）
Clean it up
2、Mechanical Part
Problem Cause Solution
The image can
not focus when
using high
magnification
objective
The specimen is
placed inversely
The coverslip is too
thick
Turn inversely
Use the standard
coverslip（0.17 mm）
The objective
and the
specimen touch
when change the
low magnification
to the higher
magnification
The specimen is
placed inversely
The coverslip is too
thick
Turn inversely
Use the standard
coverslip（0.17 mm）
The specimen
is not easy to
move
The specimen
holder is not fixed Fix it
The eyes is too
tired
No diopter
adjustment
Adjust the diopter
correctly
The brightness is not
suitable
Adjust the voltage of
the lamp
3、Electrical Part
Problem Cause Solution
The lamp can’t
light when
turning on the
switch
No power
Check the
connection of the
power cord
The bulb is not
inserted Insert it correctly
The bulb burns out Replace it
The lamp burns
out suddenly
Use an unstandard
lamp
The voltage is too
high
Use the specified
lamp to replace, if
the problem is not
solved, contact with
the service
department
The brightness
is not enough
Use a substandard
lamp
Use the specified
lamp
The voltage is too
low Add the voltage
The bulb flickers
or the brightness
is vertiginous
The bulb is going to
burn out Replace it