Organomation N-EVAP 11645 User manual

N-EVAP ™
Nitrogen Evaporation System
Models 11634 & 11645
DIGITAL & TIME CONTROL SYSTEM
INSTRUCTION MANUAL
ORGANOMATION ASSOCIATES INC.
266 RIVER ROAD WEST
BERLIN, MA 01503
U. S. A.
Tel: [phone] Fax: [phone]

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TABLE OF CONTENTS
N-EVAPTM
Forward - Letter from the President
Introduction
Instrument Items Shipped
Instrument Description
Instrument Part Identification
Safety Considerations - Read before operation
Installation
Location
Bath Setup
Instrument Setup
Operation
Instrument Control Identification
Planning and Preparation
Bath Operation
Instrument Operation
Optimization
Maintenance and Cleaning
Trouble Shooting
Technical Information
Parts List
Service and Returns
Shipping - Claims for damage or shortage
Specifications
Wiring Diagrams
Contacting Organomation and its Representatives
Product designs are subject to change without notice
V22.5

V22.5 - 1 -
INTRODUCTIONN-EVAP
Items Shipped
Carefully check the contents of all cartons received for damage which may have
occurred in transit. Retain all cartons and packaging materials until all components have been
checked against the packing slip, the component list below, and the equipment has been
assembled and tested. Contact Organomation Associates Inc. immediately if any damage or
discrepancies are found.
Your shipment should contain one or more of the instruments shown below. Option codes are
listed on the next page.
Instrument Size
34 Position N-EVAP Nitrogen evaporation system
45 Position N-EVAP Nitrogen evaporation system
Flow meter Assembly with Mounting Bracket & Connector Tube
0-30 LPM for 34/45 position N-EVAP
19 ga. x 4” Stainless Steel Luer Lock Needles, blunt end
34 or 45 each for 34 / 45 Position N-EVAP
OA-HEAT Water Bath 1400W, for 34 / 45 position N-EVAP
SS Adapter Tube 1/4” (7 mm) OD
T-Handle Hex Key adjustment tools
1 ea. 3/16” x 8” Long, for 30/45 position N-EVAP
Manual for N-EVAP models 11634, 11645
Pasteur Pippet Adapter with flow controller, 1 Dozen per set (Optional)
Cat #
11634
11645
NA1421
NA0603
B1602
V11291
V11437
V10124
NA0636
N-EVAP and OA-HEAT are Trademarks of Organomation Associates Inc.

V22.5 - 2 -
INTRODUCTION
N-EVAP ™
Option Codes and additional items shipped
The following list contains option codes and items which may have been shipped in
conjunction with the standard parts shown on the previous page. Please check your packing
list and order information carefully to determine if these items are included in your shipment.
For a complete list of available accessories, please refer to the Accessories Section.
Your shipment may contain the following optional items:
Description
34 or 45 position N-EVAP Instrument ordered without OA-HEAT water bath,
includes a flow meter mounting bracket (included with flow meter) for direct
mounting to stand base versus to the bath.
Pasteur Pipit Fittings replace SS needles and Luer fittings on the N-EVAP. 34, or
45 pieces are provided with the respective N-EVAP size ordered, reference part #
NA0636
34 or 45 position N-EVAP Instrument and OA-HEAT water bath are coated in
PTFE. Instrument is black in color. The water bath exterior is blue and the pan is
black.
SS Needles 19 gauge x 4” (100mm) Long, are coated in PTFE and are black in
color.
OA-HEAT water bath has been modified for the Type-Z Purge Positive Pressure
bath option. Additional parts include: differential pressure gauge, mounting
bracket, and tubing.
OA-HEAT water bath is wired as a 240 volt unit.
Option
-O
-P
-RT
-T
-Z
-2

V22.5 - 3 -
INTRODUCTION
N-EVAP ™
Instrument Description
The N-EVAP Nitrogen Evaporation System is designed for general evaporation and / or
concentration of analytical or biological samples in a variety of test tube based glassware under
controlled and reproducible conditions.
The complete instrument is shown below.
Figure 1

V22.5 - 4 -
INTRODUCTION
N-EVAP ™
Item
(1)
(2)
(3)
(4)
(5)
(6)
(7)
(8)
(9)
(10)
(11)
(12)
(13)
(14)
(15)
(16)
(17)
(18)
(19)
(20)
Part Name
Water Bath
Stand
Support Tray
Sample Spring
Sample Holder
Filter
Hoist Assembly
Swivel Fitting
Gas Tube
Silicone Tubing
Needle Valve
Valve Tube Assembly
Luer Fitting
Blunt Needle
PTFE coated
(Optional)
Obsolete
Center Tube Assem.
Flow meter / Bracket
Regulator & Gauge
Control System
Connector Tube
Description
Round water bath, provides heat.
Supports N-EVAP in bath or bench.
Supports test tube samples.
Holds and centers samples.
Holds various sized samples.
Removes particles from gas stream.
Assists lifting of instrument
Connects gas tube for unlimited
rotation.
Delivers gas to rotating instrument.
Delivers gas to each Valve Tube
Assembly.
Adjusts gas flow to each sample.
Delivers gas to each sample.
Connects needle or Pipet Adapter.
Delivers gas into sample tube.
PTFE coated (Optional)
Allows mounted hardware to rotate.
Meters gas flow to samples.
Pressure reduction to instrument.
Digital / Time control system.
Connects gas source to instrument.
11634/45
B1602
- - - - -
V12078
P0614
P1615
NA0403
P1524
P1204
V11291
P1610
P0627
P0607
NA0603
NA0603-T
P1626
NA1421
V11985
11202
NA1101
11645
B1602
--------
V12078
P0614
P1616
NA0403
P1524
P1204
V11291
P1611
P0627
P0607
NA0603
NA0603-T
P1626
NA1421
V11985
11202
NA1101
PARTS LIST

V22.5 - 5 -
INSTALLATION
N-EVAP ™
Location
The N-EVAP Evaporator System should be located on a bench top or in a chemical
fume hood if hazardous or flammable materials and solvents are to be used. The location
should provide the necessary support services for the instrument. These include electrical
power (required for water bath) and a clean inert gas source (Air or Nitrogen). Please review
the Specifications Section for further information.
Bath Setup
1. Position the bath on a stable flat surface such as a lab bench or in a chemical fume hood.
2. Turn the bath rocker switch to the “OFF” position.
3. Turn the heat switch to the center “OFF” position.
4. Plug the bath electrical cord into a 3 wire grounded electrical outlet rated for 110-120
VAC, 50-60 Hz, single phase, 15 amps.
Optional 220 VAC baths are clearly marked and should be plugged into a 3 wire
grounded electrical outlet rated for 220-240 VAC, 50-60 Hz, single phase, 15 amps.
5. Water Bath
Fill the bath with water to within 0.5 inch of the bath rim. The use of de-ionized water
will reduce mineral build up and extend bath operational life and is recommended.
Water Only

V22.5 - 6 -
INSTALLATION
N-EVAP ™
Bath Setup (Continued)
7. Flow meter Assembly - Provided with all N-EVAP Systems. If an OA-HEAT
bath was purchased without an N-EVAP instrument, proceed to the next section.
a See figure 4 for parts identification and position.
A. Attach the flow meter and bracket to the top left rear corner of the Control Box
with the two 10/32 x 1/2” screws provided. The meter should be positioned
with the needle valve facing forward.
B. Connect the connector tube to the bulkhead fitting located in the left rear corner
corner of the control box.
C. Connect the 1/4” plastic tube from the control box to the lower fitting on the
flow meter.
D. Connect the Connector Tube to a clean gas source (Air or Nitrogen). Source
should be capable of being regulated down to 30 psig maximum.
8. Pressure Reducing Regulator is standard on all systems utilizing a side mounted control
box. This item is pre-installed onto the top of the control box immediately in front of
the flow meter. Adjustment range is 0 - 30 psig.
Figure 4

V22.5 - 7 -
INSTALLATION
N-EVAP ™
Instrument Setup
1. Place the instrument and rod into the bath or on a flat level surface. A stand plate
is available for the Model 116 as an accessory. The stand or rod is loose relative to
the instrument and must be held in place while placing the assembly into position.
2. Flow meter Assembly - for N-EVAP Systems purchased without OA-HEAT bath unit.
Please proceed to the next section if you have already set up a water bath.
A. Remove one cap nut from one of the stand feet. Secure the meter and bracket to
the foot screw and replace and tighten the cap nut.
3. Connect the silicone or SS gas line as follows:
A. Connect the SS Gas Tube into the swivel fitting at the top of the instrument.
A Insert the tube into the fitting as far as it will go, approximately 1/2 inch (13mm).
BConnect the lower end of the SS Gas Tube to the fitting on the top of the
flow meter. Secure with a wrench.
4. Loosen the Thumb Screw at the top of the unit. Raise the instrument to its highest
position, then retighten the Thumb Screw to hold the instrument in place.
5. Connect the coiled black connector tube to the fitting located on the back right corner of
the control box. For units without the OA-HEAT bath unit, connect directly to
a the lower fitting on the flow meter Press firmly into place.
6. Adjustments for test tube sizes above or below 100mm length as follows:
A. For test tube lengths between 25 and 100mm length, the lower support tray may
a be raised by turning each of the three thumb screws located on the upper surface
a of the sample holder plate. Rotate clockwise to raise the support plate and a
a counterclockwise to lower it. For mixed test tube sizes, the support plate may
------ be adjusted in a “tilted” position.
C. For test tubes greater than 100 mm length or if Pasteur pipet adapters are used,
a the top plate may be adjusted upwards to its second position on the center tube.
Locate the small hole approximately 35 mm above the manifold in the center
tube. Loosen the set screws in the manifold and slide the top plate assembly a
on upwards until one set screw is over the upper hole. Retighten the set screws.

V22.5 - 8 -
INSTALLATION
N-EVAP ™
Instrument Setup (Continued)
D. Sample Holder Plate Adjustment - This item may need to be adjusted from time
to time as follows:
1. Loosen the set screws located in the white collar above the plate .
2. Raise the sample holder to the desired height, align with the Valve Tubes
to center the needles, and secure in place with the set screws.
7. Needles and Pipets - SS Luer Lock Needles are supplied with the standard N-EVAP
system. Optional Pasteur Pipet Adapters allow the use of glass pipets. These adapters
replace the SS Needles on the N-EVAP. Pasteur Pipet Adapters allow the use of
both needles and pipets.
A. SS Needles - Install into the Luer fitting at the bottom of the Valve Tube
assembly by rotating the needle 1/2 turn. Do not over tighten, finger tight only.
B. Pasteur Pipet “Push-On” Adapters - Push the pipet onto the end of the adapter.
The tubing on the end of the adapter will make a gas seal. Two sizes of tubing
are provided for larger or smaller diameter pipets. Once the pipet is on the
adapter, screw the adapter into the luer fitting on the instrument just like a
needle.
8. Raise all Valve Tubes to their highest position. The plastic tube nuts on the top plate
should be adjusted so that the Valve Tubes slide easily by hand, but do not fall when
released.
8. Turn all needle valves on the Valve Tube Assembly off by rotating clockwise.
9. Close the valve on the flow meter by rotating clockwise.
10. Install needles or pipets as required.

V22.5 - 9 -
SAFETY
N-EVAP ™
Safety Considerations
READ THIS SECTION BEFORE EQUIPMENT OPERATION!
This equipment is designed for use in the Analytical or Environmental Laboratory by
trained laboratory personnel for evaporative applications. Use of this equipment beyond
its stated intended purpose and operating parameters is not recommended and will be the sole
responsibility of the user. This equipment should not be modified or altered. Organomation
Associates, Inc. assumes no liability for any misuse of or modification to this product and such
misuse or modification will immediately void all warranties.
This equipment should be used in accordance with the operating instructions contained in this
manual. For alternative uses not covered in this manual, please contact Organomation
Associates technical department for product suitability, safety, and alternative operating
instructions.
The following are general safety guidelines recommended when using this product. Please
consult your laboratory safety officer for any additional safety steps which may be necessary
for your specific application or material.
1. Thoroughly review your MSDS (Material Safety Data Sheets) for all chemicals to be
used with this equipment.
2. Do not use this equipment with materials with auto ignition points below 210 ºC.
3. Hand and eye protection are required when using this product. Additional protection
may be required with respect to the materials being used. Please consult your
laboratory safety officer.
4. This product should only be used in a chemical fume hood with adequate ventilation.
5. Do not move the product when hot. Scalding from bath water or media may result.
6. Do not open bath enclosure while energized - SHOCK HAZARD!
7. Repairs of electrical components should be conducted by a trained electrical technician.
Incorrect replacement parts or assembly may damage the product and create a serious
safety hazard for the user. Factory repair is highly recommended.
8. The use of samples containing ether based, fuel, munitions, or other extremely
flammable or explosive materials, compounds, or residues should not be used in
this equipment without significant additional safety precautions being taken.
Contact Organomation Associates Inc. prior to any such use.
9. Use of acidic or base materials may damage this product is are not recommended
unless the product was ordered with the optional protective coating in PTFE

SAFETY
N-EVAP ™
Planning and Preparation
It is important to thoroughly understand the procedures and equipment operation prior to
the use of the equipment. High speed Nitrogen evaporation requires a balance of sample
volume, Nitrogen flow, bath temperature, needle position and adjustment. Improper use can
impair performance, contaminate samples or result in loss of samples. Environmental
conditions are also important, examples include use of dry or wet heating media, hood airborne
contaminates, gas purge purity, and sample handling procedures. If you are unfamiliar with the
use of the N-Evap System or are working with a new procedure, it is recommended that a trial
run be made using a sample blank to determine optimal operating conditions.
The N-EVAP System is designed to handle multiple samples simultaneously up to the capacity
of the equipment. Glass or plastic tubes from 10 to 30mm OD and up to 150mm height may be
accommodated. Centrifuge tubes, scintillation vials, small beakers, and Erlenmeyers (50 ml)
may also be used. Choice of SS needles or disposable glass pipets (fittings) are available.
The N-EVAP System is manufactured utilizing inert materials. The FDA approved tubing
used in the gas distribution system is 100% free of Phthalate presence.
WARNING!!!
The use of samples containing ether based, fuel, munitions, or other extremely flammable
or explosive materials, compounds, or residues should not be used in this equipment.
The use of OAI equipment in any hazardous location or with hazardous materials is not recom-
mended, endorsed, or warranted by OAI and any such use is at the sole discretion and is the
responsibility of the user. A TYPE -Z Purged Positive Pressure Bath Option is available for
additional safety.
Even equipped with this option, either passive or active, extreme care and caution must be
exercised when using these materials. The equipment must be placed in a location with
adequate ventilation and safe guards, recommendations include fire suppression system, shatter
proof glass, and adequate shielding for personnel. No other electronic devices should in the
same location unless they are either Z-Purge protected or are explosion proof. No flammable
solvents should be stored in this location. Materials capable of forming peroxides prior to or
during evaporation must be stabilized with sufficient anti-oxidant or they should not be used.
Under no circumstances should this equipment be used with materials capable of auto ignition
below 210 Degrees Centigrade or with materials containing peroxides.
The Type Z Purged Positive Pressure Bath Option meets the requirements as set forth by the
National Fire Protection Association (NFPA) for electrical equipment in Class 1, Division I and
II locations. OAI equipment has not been tested to any safety standard for use in hazard-
ous locations or with hazardous materials. No endorsements have been made or given by
the NFPA or Underwriters Laboratories (UL) as regards OAI equipment or its use in this
regard. Please Contact Organomation Associates Technical Support if you have any questions
concerning the use of TYPE-Z Purged equipment or questionable materials in OAI equipment.
V22.5 - 10 -

V22.5 - 11 -
OPERATION
N-EVAP ™
Instrument Controls - Main Control Panel
Digital Controller - Controls bath temperature
Timer Controller - Controls timed functions when selected. Used for bath preheat
or automated control of services.
Time Switch - Starts or resets timed operation, momentary switch.
Heat Switch - Selects manual or timed control of heat system.
Gas Switch - Selects manual or timed control of gas system.
Time Light - Green, indicates timed operation in progress.
Alert Light - Red, indicates timed operation complete or inactive.
Heat Light - Amber, indicates heat system is active.
Gas Light - Amber, indicates gas system is active.
ON / OFF Switch
Green Lighted Rocker
ALERT Indicator
Red Light
TIME System ON
Green Light
HEAT System ON
Amber Light
GAS System ON
Amber Light
HEAT Switch
ON / OFF / ON
GAS Switch
ON / OFF / ON
Manual Control
Timed Control
TIME Controller
Digital Temperature
Controller
Function Button
Adjustment Buttons
RESET Button
MODE/Settings Menu Button
Directional Buttons
TIME Switch
Start / Null / Reset

V22.5 - 12 -
OPERATION
N-EVAP ™
Bath Operation
1. Press the reset button on the GFCI (if present).
2. Turn the bath rocker switch on.
3. Digital Electronic Control - Adjust the digital controller to the desired temperature
set point.
The controller set point may be adjusted by depressing the “*” on the front panel and
depressing the up or down arrow keys to the desired temperature. Release the “*” key
when the temperature desired is shown on the display. This setting will be retained
even after the system is turned off.
To view the current set point, depress and hold the “*” key. Release when done.
Digital Controllers are programmed by Organomation for maximum operating set point
as follows:
Water Baths 100ºC
Note: The controller set point can be set above the maximum heating capability of the
bath it is mounted in.
Example: Setting water bath temperature above 75°C when floats are not present.
Doing so will cause the bath to operate continually at 100% heat without temperature
control. This type of operation defeats the purpose of the controller and is not
recommended.
Over-Temperature
Temperature is controlled by a Digital Temperature Controller. All Organomation heating
systems utilize a High Temperature Protection Switch to protect against temperature control
failure. In the event of temperature control failure, the heating unit will operate at failsafe tem-
perature levels. Equipment should be monitored periodically during use to ensure user selected
parameters are being maintained. A red blinking light on the digital controller will activate and
blink if the temperature exceeds 10°C above the user set point. Should abnormal temperature
values be observed, turn the unit off. Unplug it from the wall. Contact Organomation Tech-
nical Department prior to any further use of the equipment.
Do not operate the equipment if loss of temperature control is detected.

V22.5 - 13 -
OPERATION
N-EVAP ™
Bath Operation
4. Manual Gas and Heat Control
A) To operate the heat system manually, depress the heat switch to the lower posi
tion. The Amber heat light will glow indicating the heat system is active.
Temperature will be controlled by the digital temperature controller.
B) To operate the gas system manually, depress the gas switch to the lower position.
The Amber gas light will glow indicating the gas system is active. The gas
system is controlled by a solenoid valve located within the control box. When
active the solenoid is open allowing gas flow to the flow meter and subsequently
to the needle valves on the instrument.
C) To turn off either service, simply return their respective switches to the middle
“OFF” position.
5. Timed Gas and Heat Control
The Time control system allows several operating modes which can be set for different
operations. The timer may be used to preheat the bath in the morning prior to use, to
shut down the system after a specific period of time (unattended operation), or for non-
dryness endpoint operations. Only timer functions ond.1 and ofd are functional on
this system.
Operation found on next page.

V22.5 - 14 -
OPERATION
N-EVAP ™
Bath Operation
5. Timed Gas and Heat Control
The time control system allows several operating modes which can be set for different
operations. The timer may be used to preheat the bath in the morning prior to use, to shut
down the system after a specific period of time (unattended operation), or for non- dry
ness endpoint operations.
Preheat Bath Timed Operation
To begin heating the bath automatically prior to use the following morning, please follow
the procedures below.
A) Fill the bath with water to within 0.5 inches of the bath rim or up to the overflow
port if so equipped.
B) Turn the bath on.
C) Hold the MODE button until menu screen appears.
D) Screen to read “out.n” to select output operation mode, use directional arrows
to select program “ond.1” (On Delay). Hit MODE once to continue to select
time range.
E) Select time range under “t.rnG”, use directional arrows to determine the
desired range using h (hour), m (minute), s (second).
Hold MODE to return to standby.
Other menu options are not vital to the Preheat Bath process.
F) In standby, calculate the amount of time that will elapse from the point the bath will
be left until it will be needed the following day less 1 hour for heat up time.
Example: You leave at 5:30 PM and plan to arrive at 7:00 AM and want the
bath heated and ready to go.
Elapsed time is 13.5 hours less 1 hour for heating leaves 12.5
hours.
Use directional arrows to set desired time, for this example: 12h30m.
Hit MODE once to confirm time.
G) Set the heat switch to timed operation.
H) Set the gas switch to the middle position (OFF).
I) Press the start switch to begin the timed countdown operation. At the
completion of the operation the bath heat will be turned on automatically.
NOTE: To stop a timed run at any point simply push the reset switch.

V22.5 - 15 -
OPERATION
N-EVAP ™
Bath Operation (continued)
5. Timed Gas and Heat Control (Continued)
Automatic shutdown of gas or heat services - unattended operation
To shut down the bath services after a period of time during a sample evaporative run,
ideal when each sample is to be taken to dryness and the operator will not be present at
the completion of the run. Please follow the procedures below.
A) Hold the MODE button to enter menu screen.
B) Screen to read “out.n” to select output operation mode, use directional arrows
to select program “ofd” (Off Delay). Hit MODE once to continue to select time
range.
C) Select time range under “t.rnG”, use directional arrows to determine the
desired range using h (hour), m (minute), s (second).
Hold MODE to return to standby.
Other menu options are not vital to the Preheat Bath process.
D) In standby, use directional arrows to set time that is most ideal for the
period necessary to process the sample. As most samples will vary slightly in their
evaporative times to dryness, an addition period of time should be allowed prior
to shut down. Ten to twenty minutes should be adequate.
Example: You wish to process 18 samples in 16 x 100mm test tubes to
dryness. Experience has shown that this takes approximately 25
minutes to complete. As you are expected in a meeting which
may take an hour or more, you should use the automatic time
system as there is no need to waste unnecessary gas flow:
Evaporation time is 25 minutes plus 10 minutes for variance, this
gives 35 minutes. Set the timer as follows: 35m00s
Hit MODE once to confirm time when set.
E) Set the heat switch to timed operation or manual to bypass timed control.
F) Set the gas switch to the timed position.
G) Follow instrument instruction procedures for flow meter and needle valve
settings.
H) Press the start switch to begin the timed countdown operation. At the
completion of the operation the gas flow will be turned off automatically.
NOTE: To stop a timed run at any point simply push the reset switch.

V22.5 - 16 -
OPERATION
N-EVAP ™
Bath Operation
5. Timed Gas and Heat Control (Continued)
Non-dryness automated endpoint - unattended operation
The N-EVAP system my be used for timed non-dryness endpoint evaporation. In order
for this operation to be successful the following parameters must be maintained.
Test Tube Size - All tubes must be the same size
Sample Volume - All sample volumes must be equal
Gas Flow Rate - Must be the same per sample / per position / per run
Bath temperature - The same set point must be used for each run
A trial run must be made to determine the time required to obtain the specific endpoint
desired. Careful notes should be maintained on all operating conditions. Sample size,
volume, and gas flow are the most critical, with gas flow being the most difficult to
control. Please follow the procedures outlined in the previous section for operation of
the timed system with unattended operation and shut down.
The following procedures will help to improve accuracy for each run.
A) Set the pressure regulator to a position high enough to meet all flow require-
ments 20 - 30 PSIG should be sufficient for most applications.
B) Open each valve tube with a sample 1.5 revolutions.
C) Gas flow to each position should be increased to 1 LPM / Position.
D) Samples should be equally distributed around the bath.
E) Position each needle or pipet at the same distance from the sample surface. This
position should not be altered during the run and should be duplicated on
successive runs.
F) Maintain a log of all operating conditions and positions for future use.
Experimentation has demonstrated that evaporation rate at a given temperature range is largely a
function of gas flow rate, 92%. Minor fluctuation in temperature +/- 2 degrees Celsius have
little impact on rate, less than 8%. At endpoint, evaporative losses by heat alone are
negligible for short periods of time, 10 - 30 minutes, except for very volatile compounds.